Fertility and Iron Bioaccumulation in Drosophila melanogaster Fed with Magnetite Nanoparticles Using a Validated Method

Autores
Pilaquinga, Fernanda; Cardenas Alcoser, Elena Sofia; Vela, Doris; Jara, Eliza; Morey, Jeroni; Gutiérrez Coronado, José Luis; Debut, Alexis; Piña, María de las Nieves
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Research on nanomaterial exposure-related health risks is still quite limited; this includes standardizing methods for measuring metals in living organisms. Thus, this study validated an atomic absorption spectrophotometry method to determine fertility and bioaccumulated iron content in Drosophila melanogaster flies after feeding them magnetite nanoparticles (Fe3O4NPs) dosed in a culture medium (100, 250, 500, and 1000 mg kg−1). Some NPs were also coated with chitosan to compare iron assimilation. Considering both accuracy and precision, results showed the method was optimal for concentrations greater than 20 mg L−1. Recovery values were considered optimum within the 95–105% range. Regarding fertility, offspring for each coated and non-coated NPs concentration decreased in relation to the control group. Flies exposed to 100 mg L−1 of coated NPs presented the lowest fertility level and highest bioaccumulation factor. Despite an association between iron bioaccumulation and NPs concentration, the 500 mg L−1 dose of coated and non-coated NPs showed similar iron concentrations to those of the control group. Thus, Drosophila flies’ fertility decreased after NPs exposure, while iron bioaccumulation was related to NPs concentration and coating. We determined this method can overcome sample limitations and biological matrix-associated heterogeneity, thus allowing for bioaccumulated iron detection regardless of exposure to coated or non-coated magnetite NPs, meaning this protocol could be applicable with any type of iron NPs.
Fil: Pilaquinga, Fernanda. Pontificia Universidad Católica del Ecuador; Ecuador
Fil: Cardenas Alcoser, Elena Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Gobierno de la Ciudad de Buenos Aires. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Fundación de Endocrinología Infantil. Centro de Investigaciones Endocrinológicas "Dr. César Bergada"; Argentina. Pontificia Universidad Católica del Ecuador; Ecuador
Fil: Vela, Doris. Pontificia Universidad Católica del Ecuador; Ecuador
Fil: Jara, Eliza. Pontificia Universidad Católica del Ecuador; Ecuador
Fil: Morey, Jeroni. University of the Balearic Islands; España
Fil: Gutiérrez Coronado, José Luis. Universidad Autónoma de Madrid; España
Fil: Debut, Alexis. Universidad de Las Fuerzas Armadas; Ecuador
Fil: Piña, María de las Nieves. University of the Balearic Islands; España
Materia
CHITOSAN
DROSOPHILA MELANOGASTER
FERTILITY
IRON BIOACCUMULATION
MAGNETITE NANOPARTICLES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/170710
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Fertility and Iron Bioaccumulation in Drosophila melanogaster Fed with Magnetite Nanoparticles Using a Validated MethodPilaquinga, FernandaCardenas Alcoser, Elena SofiaVela, DorisJara, ElizaMorey, JeroniGutiérrez Coronado, José LuisDebut, AlexisPiña, María de las NievesCHITOSANDROSOPHILA MELANOGASTERFERTILITYIRON BIOACCUMULATIONMAGNETITE NANOPARTICLEShttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Research on nanomaterial exposure-related health risks is still quite limited; this includes standardizing methods for measuring metals in living organisms. Thus, this study validated an atomic absorption spectrophotometry method to determine fertility and bioaccumulated iron content in Drosophila melanogaster flies after feeding them magnetite nanoparticles (Fe3O4NPs) dosed in a culture medium (100, 250, 500, and 1000 mg kg−1). Some NPs were also coated with chitosan to compare iron assimilation. Considering both accuracy and precision, results showed the method was optimal for concentrations greater than 20 mg L−1. Recovery values were considered optimum within the 95–105% range. Regarding fertility, offspring for each coated and non-coated NPs concentration decreased in relation to the control group. Flies exposed to 100 mg L−1 of coated NPs presented the lowest fertility level and highest bioaccumulation factor. Despite an association between iron bioaccumulation and NPs concentration, the 500 mg L−1 dose of coated and non-coated NPs showed similar iron concentrations to those of the control group. Thus, Drosophila flies’ fertility decreased after NPs exposure, while iron bioaccumulation was related to NPs concentration and coating. We determined this method can overcome sample limitations and biological matrix-associated heterogeneity, thus allowing for bioaccumulated iron detection regardless of exposure to coated or non-coated magnetite NPs, meaning this protocol could be applicable with any type of iron NPs.Fil: Pilaquinga, Fernanda. Pontificia Universidad Católica del Ecuador; EcuadorFil: Cardenas Alcoser, Elena Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Gobierno de la Ciudad de Buenos Aires. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Fundación de Endocrinología Infantil. Centro de Investigaciones Endocrinológicas "Dr. César Bergada"; Argentina. Pontificia Universidad Católica del Ecuador; EcuadorFil: Vela, Doris. Pontificia Universidad Católica del Ecuador; EcuadorFil: Jara, Eliza. Pontificia Universidad Católica del Ecuador; EcuadorFil: Morey, Jeroni. University of the Balearic Islands; EspañaFil: Gutiérrez Coronado, José Luis. Universidad Autónoma de Madrid; EspañaFil: Debut, Alexis. Universidad de Las Fuerzas Armadas; EcuadorFil: Piña, María de las Nieves. University of the Balearic Islands; EspañaMolecular Diversity Preservation International2021-05-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/170710Pilaquinga, Fernanda; Cardenas Alcoser, Elena Sofia; Vela, Doris; Jara, Eliza; Morey, Jeroni; et al.; Fertility and Iron Bioaccumulation in Drosophila melanogaster Fed with Magnetite Nanoparticles Using a Validated Method; Molecular Diversity Preservation International; Molecules; 26; 9; 10-5-2021; 1-151420-3049CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1420-3049/26/9/2808info:eu-repo/semantics/altIdentifier/doi/10.3390/molecules26092808info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:47:16Zoai:ri.conicet.gov.ar:11336/170710instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:47:17.207CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Fertility and Iron Bioaccumulation in Drosophila melanogaster Fed with Magnetite Nanoparticles Using a Validated Method
title Fertility and Iron Bioaccumulation in Drosophila melanogaster Fed with Magnetite Nanoparticles Using a Validated Method
spellingShingle Fertility and Iron Bioaccumulation in Drosophila melanogaster Fed with Magnetite Nanoparticles Using a Validated Method
Pilaquinga, Fernanda
CHITOSAN
DROSOPHILA MELANOGASTER
FERTILITY
IRON BIOACCUMULATION
MAGNETITE NANOPARTICLES
title_short Fertility and Iron Bioaccumulation in Drosophila melanogaster Fed with Magnetite Nanoparticles Using a Validated Method
title_full Fertility and Iron Bioaccumulation in Drosophila melanogaster Fed with Magnetite Nanoparticles Using a Validated Method
title_fullStr Fertility and Iron Bioaccumulation in Drosophila melanogaster Fed with Magnetite Nanoparticles Using a Validated Method
title_full_unstemmed Fertility and Iron Bioaccumulation in Drosophila melanogaster Fed with Magnetite Nanoparticles Using a Validated Method
title_sort Fertility and Iron Bioaccumulation in Drosophila melanogaster Fed with Magnetite Nanoparticles Using a Validated Method
dc.creator.none.fl_str_mv Pilaquinga, Fernanda
Cardenas Alcoser, Elena Sofia
Vela, Doris
Jara, Eliza
Morey, Jeroni
Gutiérrez Coronado, José Luis
Debut, Alexis
Piña, María de las Nieves
author Pilaquinga, Fernanda
author_facet Pilaquinga, Fernanda
Cardenas Alcoser, Elena Sofia
Vela, Doris
Jara, Eliza
Morey, Jeroni
Gutiérrez Coronado, José Luis
Debut, Alexis
Piña, María de las Nieves
author_role author
author2 Cardenas Alcoser, Elena Sofia
Vela, Doris
Jara, Eliza
Morey, Jeroni
Gutiérrez Coronado, José Luis
Debut, Alexis
Piña, María de las Nieves
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CHITOSAN
DROSOPHILA MELANOGASTER
FERTILITY
IRON BIOACCUMULATION
MAGNETITE NANOPARTICLES
topic CHITOSAN
DROSOPHILA MELANOGASTER
FERTILITY
IRON BIOACCUMULATION
MAGNETITE NANOPARTICLES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Research on nanomaterial exposure-related health risks is still quite limited; this includes standardizing methods for measuring metals in living organisms. Thus, this study validated an atomic absorption spectrophotometry method to determine fertility and bioaccumulated iron content in Drosophila melanogaster flies after feeding them magnetite nanoparticles (Fe3O4NPs) dosed in a culture medium (100, 250, 500, and 1000 mg kg−1). Some NPs were also coated with chitosan to compare iron assimilation. Considering both accuracy and precision, results showed the method was optimal for concentrations greater than 20 mg L−1. Recovery values were considered optimum within the 95–105% range. Regarding fertility, offspring for each coated and non-coated NPs concentration decreased in relation to the control group. Flies exposed to 100 mg L−1 of coated NPs presented the lowest fertility level and highest bioaccumulation factor. Despite an association between iron bioaccumulation and NPs concentration, the 500 mg L−1 dose of coated and non-coated NPs showed similar iron concentrations to those of the control group. Thus, Drosophila flies’ fertility decreased after NPs exposure, while iron bioaccumulation was related to NPs concentration and coating. We determined this method can overcome sample limitations and biological matrix-associated heterogeneity, thus allowing for bioaccumulated iron detection regardless of exposure to coated or non-coated magnetite NPs, meaning this protocol could be applicable with any type of iron NPs.
Fil: Pilaquinga, Fernanda. Pontificia Universidad Católica del Ecuador; Ecuador
Fil: Cardenas Alcoser, Elena Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Gobierno de la Ciudad de Buenos Aires. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Fundación de Endocrinología Infantil. Centro de Investigaciones Endocrinológicas "Dr. César Bergada"; Argentina. Pontificia Universidad Católica del Ecuador; Ecuador
Fil: Vela, Doris. Pontificia Universidad Católica del Ecuador; Ecuador
Fil: Jara, Eliza. Pontificia Universidad Católica del Ecuador; Ecuador
Fil: Morey, Jeroni. University of the Balearic Islands; España
Fil: Gutiérrez Coronado, José Luis. Universidad Autónoma de Madrid; España
Fil: Debut, Alexis. Universidad de Las Fuerzas Armadas; Ecuador
Fil: Piña, María de las Nieves. University of the Balearic Islands; España
description Research on nanomaterial exposure-related health risks is still quite limited; this includes standardizing methods for measuring metals in living organisms. Thus, this study validated an atomic absorption spectrophotometry method to determine fertility and bioaccumulated iron content in Drosophila melanogaster flies after feeding them magnetite nanoparticles (Fe3O4NPs) dosed in a culture medium (100, 250, 500, and 1000 mg kg−1). Some NPs were also coated with chitosan to compare iron assimilation. Considering both accuracy and precision, results showed the method was optimal for concentrations greater than 20 mg L−1. Recovery values were considered optimum within the 95–105% range. Regarding fertility, offspring for each coated and non-coated NPs concentration decreased in relation to the control group. Flies exposed to 100 mg L−1 of coated NPs presented the lowest fertility level and highest bioaccumulation factor. Despite an association between iron bioaccumulation and NPs concentration, the 500 mg L−1 dose of coated and non-coated NPs showed similar iron concentrations to those of the control group. Thus, Drosophila flies’ fertility decreased after NPs exposure, while iron bioaccumulation was related to NPs concentration and coating. We determined this method can overcome sample limitations and biological matrix-associated heterogeneity, thus allowing for bioaccumulated iron detection regardless of exposure to coated or non-coated magnetite NPs, meaning this protocol could be applicable with any type of iron NPs.
publishDate 2021
dc.date.none.fl_str_mv 2021-05-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/170710
Pilaquinga, Fernanda; Cardenas Alcoser, Elena Sofia; Vela, Doris; Jara, Eliza; Morey, Jeroni; et al.; Fertility and Iron Bioaccumulation in Drosophila melanogaster Fed with Magnetite Nanoparticles Using a Validated Method; Molecular Diversity Preservation International; Molecules; 26; 9; 10-5-2021; 1-15
1420-3049
CONICET Digital
CONICET
url http://hdl.handle.net/11336/170710
identifier_str_mv Pilaquinga, Fernanda; Cardenas Alcoser, Elena Sofia; Vela, Doris; Jara, Eliza; Morey, Jeroni; et al.; Fertility and Iron Bioaccumulation in Drosophila melanogaster Fed with Magnetite Nanoparticles Using a Validated Method; Molecular Diversity Preservation International; Molecules; 26; 9; 10-5-2021; 1-15
1420-3049
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1420-3049/26/9/2808
info:eu-repo/semantics/altIdentifier/doi/10.3390/molecules26092808
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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