Application of bacteriocins from Enterococcus hirae on butterhead lettuce seeds inoculated with Escherichia coli O157:H7

Autores
Goñi, María Gabriela; Tomadoni, Bárbara María; Audisio, Marcela Carina; Ibarguren, Carolina; Roura, Sara Ines; Moreira, Maria del Rosario; Ponce, Alejandra Graciela
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Several outbreaks of Escherichia coli O157:H7 infections have been associated with contaminated seeds and sprouts. Effective procedures to control pathogenic bacteria on seeds destined for sprout production should be developed to ensure an acceptable microbiological quality. In this study, the use of bacteriocins produced by Enterococcus hirae (Eh9) was analyzed as a potential method to control the endogenous microbial populations of lettuce seeds and avoid pathogen contamination on seeds destined for sprout production. Seeds decontamination with Eh9 cell-free supernatant (CFS) achieved a significative fungiostatic effect without affecting germination. CFS treatment did not exert a biocontrol effect on E. coli endogenous; however, a significant bacteriocin biocontrol was reached on lettuce seeds inoculated with exogenous E. coli during refrigerated storage (7 days). Germination percentage and potential yield were not affected by the treatments. A better understanding of the behavior of pathogens in preharvest environments will assure the delivery of safe produce to the consumer.
Fil: Goñi, María Gabriela. Universidad Nacional de La Plata. Facultad de Ingenierí­a. Departamento de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tomadoni, Bárbara María. Universidad Nacional de La Plata. Facultad de Ingenierí­a. Departamento de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Audisio, Marcela Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina
Fil: Ibarguren, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina
Fil: Roura, Sara Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ingenierí­a. Departamento de Ingeniería Química; Argentina
Fil: Moreira, Maria del Rosario. Universidad Nacional de La Plata. Facultad de Ingenierí­a. Departamento de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ponce, Alejandra Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ingenierí­a. Departamento de Ingeniería Química; Argentina
Materia
ENTEROCOCCUS HIRAE
BACTERIOCINS
BIOPROTECTORS
FOOD PATHOGENS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/65651
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/65651
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Application of bacteriocins from Enterococcus hirae on butterhead lettuce seeds inoculated with Escherichia coli O157:H7Goñi, María GabrielaTomadoni, Bárbara MaríaAudisio, Marcela CarinaIbarguren, CarolinaRoura, Sara InesMoreira, Maria del RosarioPonce, Alejandra GracielaENTEROCOCCUS HIRAEBACTERIOCINSBIOPROTECTORSFOOD PATHOGENShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Several outbreaks of Escherichia coli O157:H7 infections have been associated with contaminated seeds and sprouts. Effective procedures to control pathogenic bacteria on seeds destined for sprout production should be developed to ensure an acceptable microbiological quality. In this study, the use of bacteriocins produced by Enterococcus hirae (Eh9) was analyzed as a potential method to control the endogenous microbial populations of lettuce seeds and avoid pathogen contamination on seeds destined for sprout production. Seeds decontamination with Eh9 cell-free supernatant (CFS) achieved a significative fungiostatic effect without affecting germination. CFS treatment did not exert a biocontrol effect on E. coli endogenous; however, a significant bacteriocin biocontrol was reached on lettuce seeds inoculated with exogenous E. coli during refrigerated storage (7 days). Germination percentage and potential yield were not affected by the treatments. A better understanding of the behavior of pathogens in preharvest environments will assure the delivery of safe produce to the consumer.Fil: Goñi, María Gabriela. Universidad Nacional de La Plata. Facultad de Ingenierí­a. Departamento de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Tomadoni, Bárbara María. Universidad Nacional de La Plata. Facultad de Ingenierí­a. Departamento de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Audisio, Marcela Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; ArgentinaFil: Ibarguren, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; ArgentinaFil: Roura, Sara Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ingenierí­a. Departamento de Ingeniería Química; ArgentinaFil: Moreira, Maria del Rosario. Universidad Nacional de La Plata. Facultad de Ingenierí­a. Departamento de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ponce, Alejandra Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ingenierí­a. Departamento de Ingeniería Química; ArgentinaUniversiti Putra Malaysia2016-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/65651Goñi, María Gabriela; Tomadoni, Bárbara María; Audisio, Marcela Carina; Ibarguren, Carolina; Roura, Sara Ines; et al.; Application of bacteriocins from Enterococcus hirae on butterhead lettuce seeds inoculated with Escherichia coli O157:H7; Universiti Putra Malaysia; International Food Research Journal; 23; 6; 12-2016; 2653-26601985-46682231 754CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.ifrj.upm.edu.my/23%20(06)%202016/(48).pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:40Zoai:ri.conicet.gov.ar:11336/65651instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:40.363CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Application of bacteriocins from Enterococcus hirae on butterhead lettuce seeds inoculated with Escherichia coli O157:H7
title Application of bacteriocins from Enterococcus hirae on butterhead lettuce seeds inoculated with Escherichia coli O157:H7
spellingShingle Application of bacteriocins from Enterococcus hirae on butterhead lettuce seeds inoculated with Escherichia coli O157:H7
Goñi, María Gabriela
ENTEROCOCCUS HIRAE
BACTERIOCINS
BIOPROTECTORS
FOOD PATHOGENS
title_short Application of bacteriocins from Enterococcus hirae on butterhead lettuce seeds inoculated with Escherichia coli O157:H7
title_full Application of bacteriocins from Enterococcus hirae on butterhead lettuce seeds inoculated with Escherichia coli O157:H7
title_fullStr Application of bacteriocins from Enterococcus hirae on butterhead lettuce seeds inoculated with Escherichia coli O157:H7
title_full_unstemmed Application of bacteriocins from Enterococcus hirae on butterhead lettuce seeds inoculated with Escherichia coli O157:H7
title_sort Application of bacteriocins from Enterococcus hirae on butterhead lettuce seeds inoculated with Escherichia coli O157:H7
dc.creator.none.fl_str_mv Goñi, María Gabriela
Tomadoni, Bárbara María
Audisio, Marcela Carina
Ibarguren, Carolina
Roura, Sara Ines
Moreira, Maria del Rosario
Ponce, Alejandra Graciela
author Goñi, María Gabriela
author_facet Goñi, María Gabriela
Tomadoni, Bárbara María
Audisio, Marcela Carina
Ibarguren, Carolina
Roura, Sara Ines
Moreira, Maria del Rosario
Ponce, Alejandra Graciela
author_role author
author2 Tomadoni, Bárbara María
Audisio, Marcela Carina
Ibarguren, Carolina
Roura, Sara Ines
Moreira, Maria del Rosario
Ponce, Alejandra Graciela
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ENTEROCOCCUS HIRAE
BACTERIOCINS
BIOPROTECTORS
FOOD PATHOGENS
topic ENTEROCOCCUS HIRAE
BACTERIOCINS
BIOPROTECTORS
FOOD PATHOGENS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Several outbreaks of Escherichia coli O157:H7 infections have been associated with contaminated seeds and sprouts. Effective procedures to control pathogenic bacteria on seeds destined for sprout production should be developed to ensure an acceptable microbiological quality. In this study, the use of bacteriocins produced by Enterococcus hirae (Eh9) was analyzed as a potential method to control the endogenous microbial populations of lettuce seeds and avoid pathogen contamination on seeds destined for sprout production. Seeds decontamination with Eh9 cell-free supernatant (CFS) achieved a significative fungiostatic effect without affecting germination. CFS treatment did not exert a biocontrol effect on E. coli endogenous; however, a significant bacteriocin biocontrol was reached on lettuce seeds inoculated with exogenous E. coli during refrigerated storage (7 days). Germination percentage and potential yield were not affected by the treatments. A better understanding of the behavior of pathogens in preharvest environments will assure the delivery of safe produce to the consumer.
Fil: Goñi, María Gabriela. Universidad Nacional de La Plata. Facultad de Ingenierí­a. Departamento de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tomadoni, Bárbara María. Universidad Nacional de La Plata. Facultad de Ingenierí­a. Departamento de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Audisio, Marcela Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina
Fil: Ibarguren, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina
Fil: Roura, Sara Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ingenierí­a. Departamento de Ingeniería Química; Argentina
Fil: Moreira, Maria del Rosario. Universidad Nacional de La Plata. Facultad de Ingenierí­a. Departamento de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ponce, Alejandra Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ingenierí­a. Departamento de Ingeniería Química; Argentina
description Several outbreaks of Escherichia coli O157:H7 infections have been associated with contaminated seeds and sprouts. Effective procedures to control pathogenic bacteria on seeds destined for sprout production should be developed to ensure an acceptable microbiological quality. In this study, the use of bacteriocins produced by Enterococcus hirae (Eh9) was analyzed as a potential method to control the endogenous microbial populations of lettuce seeds and avoid pathogen contamination on seeds destined for sprout production. Seeds decontamination with Eh9 cell-free supernatant (CFS) achieved a significative fungiostatic effect without affecting germination. CFS treatment did not exert a biocontrol effect on E. coli endogenous; however, a significant bacteriocin biocontrol was reached on lettuce seeds inoculated with exogenous E. coli during refrigerated storage (7 days). Germination percentage and potential yield were not affected by the treatments. A better understanding of the behavior of pathogens in preharvest environments will assure the delivery of safe produce to the consumer.
publishDate 2016
dc.date.none.fl_str_mv 2016-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/65651
Goñi, María Gabriela; Tomadoni, Bárbara María; Audisio, Marcela Carina; Ibarguren, Carolina; Roura, Sara Ines; et al.; Application of bacteriocins from Enterococcus hirae on butterhead lettuce seeds inoculated with Escherichia coli O157:H7; Universiti Putra Malaysia; International Food Research Journal; 23; 6; 12-2016; 2653-2660
1985-4668
2231 754
CONICET Digital
CONICET
url http://hdl.handle.net/11336/65651
identifier_str_mv Goñi, María Gabriela; Tomadoni, Bárbara María; Audisio, Marcela Carina; Ibarguren, Carolina; Roura, Sara Ines; et al.; Application of bacteriocins from Enterococcus hirae on butterhead lettuce seeds inoculated with Escherichia coli O157:H7; Universiti Putra Malaysia; International Food Research Journal; 23; 6; 12-2016; 2653-2660
1985-4668
2231 754
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.ifrj.upm.edu.my/23%20(06)%202016/(48).pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universiti Putra Malaysia
publisher.none.fl_str_mv Universiti Putra Malaysia
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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