Genome mining and transcriptional analysis of bacteriocin genes in enterococcus faecium CRL1879

Autores
Suárez, Nadia Elina; Bonacina, Julieta; Hebert, Elvira Maria; Saavedra, Maria Lucila
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Among 151 bacterial isolates from nine artisanal cheeses, Enterococcus faecium CRL 1879 showed antibacterialactivity against the food-borne pathogen Listeria monocytogenes. The isolate produced a proteinase K-sensitivecompound in the cell free supernatant. Genome analysis demonstrated the presence of enterocin A, enterocin B,enterocin P, enterocin SE-K4-like and enterocin X biosynthetic gene clusters. Nucleotide sequences encoding for aputative two-component bacteriocin were detected using bioinformatics tools, here named enterocin CRL1879αβ.A transcriptional analysis of all bacteriocin genes by quantitative real time PCR analysis (qRT-PCR) revealed thetranscription of each enterocin gene at different levels. Finally, analysis of bacteriocin genes distribution in 251 E.faecium bioprojects was performed and compared to those identify in E. faecium CRL1879. The discriminative analysisdemonstrated that bacteriocin genes are widely distributed among Enterococcus, independently of the origin of thestrain.The results presented in this paper represent a unique finding since this is the first demonstration of an E. faeciumstrain isolated from an artisanal cheese with the complete genetic machinery to produce six classes II and one class III bacteriocins.
Fil: Suárez, Nadia Elina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Bonacina, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Hebert, Elvira Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Saavedra, Maria Lucila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Materia
BACTERIOCINS
ENTEROCOCCUS FAECIUM
GENOMES
TRANSCRIPTIONAL ANALYSIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/28258
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/28258
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genome mining and transcriptional analysis of bacteriocin genes in enterococcus faecium CRL1879Suárez, Nadia ElinaBonacina, JulietaHebert, Elvira MariaSaavedra, Maria LucilaBACTERIOCINSENTEROCOCCUS FAECIUMGENOMESTRANSCRIPTIONAL ANALYSIShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Among 151 bacterial isolates from nine artisanal cheeses, Enterococcus faecium CRL 1879 showed antibacterialactivity against the food-borne pathogen Listeria monocytogenes. The isolate produced a proteinase K-sensitivecompound in the cell free supernatant. Genome analysis demonstrated the presence of enterocin A, enterocin B,enterocin P, enterocin SE-K4-like and enterocin X biosynthetic gene clusters. Nucleotide sequences encoding for aputative two-component bacteriocin were detected using bioinformatics tools, here named enterocin CRL1879αβ.A transcriptional analysis of all bacteriocin genes by quantitative real time PCR analysis (qRT-PCR) revealed thetranscription of each enterocin gene at different levels. Finally, analysis of bacteriocin genes distribution in 251 E.faecium bioprojects was performed and compared to those identify in E. faecium CRL1879. The discriminative analysisdemonstrated that bacteriocin genes are widely distributed among Enterococcus, independently of the origin of thestrain.The results presented in this paper represent a unique finding since this is the first demonstration of an E. faeciumstrain isolated from an artisanal cheese with the complete genetic machinery to produce six classes II and one class III bacteriocins.Fil: Suárez, Nadia Elina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Bonacina, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Hebert, Elvira Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Saavedra, Maria Lucila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaOMICS International2015-10-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/28258Suárez, Nadia Elina; Bonacina, Julieta; Hebert, Elvira Maria; Saavedra, Maria Lucila; Genome mining and transcriptional analysis of bacteriocin genes in enterococcus faecium CRL1879; OMICS International; Journal of Data Mining in Genomics & Proteomics; 6; 3; 18-10-2015; 1-8;1000180-10001802153-0602CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4172/2153-0602.1000180info:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/openAccessAtribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)https://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:49Zoai:ri.conicet.gov.ar:11336/28258instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:49.758CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genome mining and transcriptional analysis of bacteriocin genes in enterococcus faecium CRL1879
title Genome mining and transcriptional analysis of bacteriocin genes in enterococcus faecium CRL1879
spellingShingle Genome mining and transcriptional analysis of bacteriocin genes in enterococcus faecium CRL1879
Suárez, Nadia Elina
BACTERIOCINS
ENTEROCOCCUS FAECIUM
GENOMES
TRANSCRIPTIONAL ANALYSIS
title_short Genome mining and transcriptional analysis of bacteriocin genes in enterococcus faecium CRL1879
title_full Genome mining and transcriptional analysis of bacteriocin genes in enterococcus faecium CRL1879
title_fullStr Genome mining and transcriptional analysis of bacteriocin genes in enterococcus faecium CRL1879
title_full_unstemmed Genome mining and transcriptional analysis of bacteriocin genes in enterococcus faecium CRL1879
title_sort Genome mining and transcriptional analysis of bacteriocin genes in enterococcus faecium CRL1879
dc.creator.none.fl_str_mv Suárez, Nadia Elina
Bonacina, Julieta
Hebert, Elvira Maria
Saavedra, Maria Lucila
author Suárez, Nadia Elina
author_facet Suárez, Nadia Elina
Bonacina, Julieta
Hebert, Elvira Maria
Saavedra, Maria Lucila
author_role author
author2 Bonacina, Julieta
Hebert, Elvira Maria
Saavedra, Maria Lucila
author2_role author
author
author
dc.subject.none.fl_str_mv BACTERIOCINS
ENTEROCOCCUS FAECIUM
GENOMES
TRANSCRIPTIONAL ANALYSIS
topic BACTERIOCINS
ENTEROCOCCUS FAECIUM
GENOMES
TRANSCRIPTIONAL ANALYSIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Among 151 bacterial isolates from nine artisanal cheeses, Enterococcus faecium CRL 1879 showed antibacterialactivity against the food-borne pathogen Listeria monocytogenes. The isolate produced a proteinase K-sensitivecompound in the cell free supernatant. Genome analysis demonstrated the presence of enterocin A, enterocin B,enterocin P, enterocin SE-K4-like and enterocin X biosynthetic gene clusters. Nucleotide sequences encoding for aputative two-component bacteriocin were detected using bioinformatics tools, here named enterocin CRL1879αβ.A transcriptional analysis of all bacteriocin genes by quantitative real time PCR analysis (qRT-PCR) revealed thetranscription of each enterocin gene at different levels. Finally, analysis of bacteriocin genes distribution in 251 E.faecium bioprojects was performed and compared to those identify in E. faecium CRL1879. The discriminative analysisdemonstrated that bacteriocin genes are widely distributed among Enterococcus, independently of the origin of thestrain.The results presented in this paper represent a unique finding since this is the first demonstration of an E. faeciumstrain isolated from an artisanal cheese with the complete genetic machinery to produce six classes II and one class III bacteriocins.
Fil: Suárez, Nadia Elina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Bonacina, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Hebert, Elvira Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Saavedra, Maria Lucila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
description Among 151 bacterial isolates from nine artisanal cheeses, Enterococcus faecium CRL 1879 showed antibacterialactivity against the food-borne pathogen Listeria monocytogenes. The isolate produced a proteinase K-sensitivecompound in the cell free supernatant. Genome analysis demonstrated the presence of enterocin A, enterocin B,enterocin P, enterocin SE-K4-like and enterocin X biosynthetic gene clusters. Nucleotide sequences encoding for aputative two-component bacteriocin were detected using bioinformatics tools, here named enterocin CRL1879αβ.A transcriptional analysis of all bacteriocin genes by quantitative real time PCR analysis (qRT-PCR) revealed thetranscription of each enterocin gene at different levels. Finally, analysis of bacteriocin genes distribution in 251 E.faecium bioprojects was performed and compared to those identify in E. faecium CRL1879. The discriminative analysisdemonstrated that bacteriocin genes are widely distributed among Enterococcus, independently of the origin of thestrain.The results presented in this paper represent a unique finding since this is the first demonstration of an E. faeciumstrain isolated from an artisanal cheese with the complete genetic machinery to produce six classes II and one class III bacteriocins.
publishDate 2015
dc.date.none.fl_str_mv 2015-10-18
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/28258
Suárez, Nadia Elina; Bonacina, Julieta; Hebert, Elvira Maria; Saavedra, Maria Lucila; Genome mining and transcriptional analysis of bacteriocin genes in enterococcus faecium CRL1879; OMICS International; Journal of Data Mining in Genomics & Proteomics; 6; 3; 18-10-2015; 1-8;1000180-1000180
2153-0602
CONICET Digital
CONICET
url http://hdl.handle.net/11336/28258
identifier_str_mv Suárez, Nadia Elina; Bonacina, Julieta; Hebert, Elvira Maria; Saavedra, Maria Lucila; Genome mining and transcriptional analysis of bacteriocin genes in enterococcus faecium CRL1879; OMICS International; Journal of Data Mining in Genomics & Proteomics; 6; 3; 18-10-2015; 1-8;1000180-1000180
2153-0602
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4172/2153-0602.1000180
info:eu-repo/semantics/altIdentifier/doi/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv OMICS International
publisher.none.fl_str_mv OMICS International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268691065995264
score 13.13397

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