Quaternized chitosan as support for the assembly of gold nanoparticles and glucose oxidase: Physicochemical characterization of the platform and evaluation of its biocatalytic acti...

Autores: Bracamonte, Maria Victoria; Bollo, Soledad; Labbé, Pierre; Rivas, Gustavo Adolfo; Ferreyra, Nancy Fabiana
Año de publicación: 2011
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: We report for the first time the use of quaternized chitosan (QCHI) for the immobilization of gold nanoparticles (NP) and glucose oxidase (GOD), the characterization of the resulting platform and its biocatalytic activity using glucose as substrate. The chemical substitution of chitosan has allowed us to work at physiologic pH to build up self-assembled layers of QCHI-NP as platform for the enzyme immobilization. The adsorption of GOD was analyzed by surface plasmon resonance (SPR) to compare the surface coverage of GOD in absence and presence of the QCHI-NP platform. The results obtained with cyclic voltammetry and scanning electrochemical microscopy (SECM) revealed that the adsorption of NP improves the conductivity of the structure and its electrochemical reactivity, facilitating the oxidation of the hydrogen peroxide produced by GOD. The electrodes modified with NP present higher amperometric response demonstrating the efficient transduction of the enzymatic activity in this structure. © 2010 Elsevier Ltd. All rights reserved.
Fil: Bracamonte, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Bollo, Soledad. Universidad de Chile; Chile
Fil: Labbé, Pierre. Universite Joseph Fourier; Francia
Fil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Ferreyra, Nancy Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Materia: Glucose Oxidase
Gold Nanoparticles
Quaternized Chitosan
Scanning Electrochemical Microscopy
Surface Plasmon Resonance
Nivel de accesibilidad: acceso abierto
Condiciones de uso: https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
Institución: Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador: oai:ri.conicet.gov.ar:11336/61612

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spelling	Quaternized chitosan as support for the assembly of gold nanoparticles and glucose oxidase: Physicochemical characterization of the platform and evaluation of its biocatalytic activityBracamonte, Maria VictoriaBollo, SoledadLabbé, PierreRivas, Gustavo AdolfoFerreyra, Nancy FabianaGlucose OxidaseGold NanoparticlesQuaternized ChitosanScanning Electrochemical MicroscopySurface Plasmon Resonancehttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1We report for the first time the use of quaternized chitosan (QCHI) for the immobilization of gold nanoparticles (NP) and glucose oxidase (GOD), the characterization of the resulting platform and its biocatalytic activity using glucose as substrate. The chemical substitution of chitosan has allowed us to work at physiologic pH to build up self-assembled layers of QCHI-NP as platform for the enzyme immobilization. The adsorption of GOD was analyzed by surface plasmon resonance (SPR) to compare the surface coverage of GOD in absence and presence of the QCHI-NP platform. The results obtained with cyclic voltammetry and scanning electrochemical microscopy (SECM) revealed that the adsorption of NP improves the conductivity of the structure and its electrochemical reactivity, facilitating the oxidation of the hydrogen peroxide produced by GOD. The electrodes modified with NP present higher amperometric response demonstrating the efficient transduction of the enzymatic activity in this structure. © 2010 Elsevier Ltd. All rights reserved.Fil: Bracamonte, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Bollo, Soledad. Universidad de Chile; ChileFil: Labbé, Pierre. Universite Joseph Fourier; FranciaFil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Ferreyra, Nancy Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaPergamon-Elsevier Science Ltd2011-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/61612Bracamonte, Maria Victoria; Bollo, Soledad; Labbé, Pierre; Rivas, Gustavo Adolfo; Ferreyra, Nancy Fabiana; Quaternized chitosan as support for the assembly of gold nanoparticles and glucose oxidase: Physicochemical characterization of the platform and evaluation of its biocatalytic activity; Pergamon-Elsevier Science Ltd; Electrochimica Acta; 56; 3; 1-2011; 1316-13220013-4686CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.electacta.2010.10.022info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0013468610013885info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:45:49Zoai:ri.conicet.gov.ar:11336/61612instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:45:49.556CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	Quaternized chitosan as support for the assembly of gold nanoparticles and glucose oxidase: Physicochemical characterization of the platform and evaluation of its biocatalytic activity
title	Quaternized chitosan as support for the assembly of gold nanoparticles and glucose oxidase: Physicochemical characterization of the platform and evaluation of its biocatalytic activity
spellingShingle	Quaternized chitosan as support for the assembly of gold nanoparticles and glucose oxidase: Physicochemical characterization of the platform and evaluation of its biocatalytic activity Bracamonte, Maria Victoria Glucose Oxidase Gold Nanoparticles Quaternized Chitosan Scanning Electrochemical Microscopy Surface Plasmon Resonance
title_short	Quaternized chitosan as support for the assembly of gold nanoparticles and glucose oxidase: Physicochemical characterization of the platform and evaluation of its biocatalytic activity
title_full	Quaternized chitosan as support for the assembly of gold nanoparticles and glucose oxidase: Physicochemical characterization of the platform and evaluation of its biocatalytic activity
title_fullStr	Quaternized chitosan as support for the assembly of gold nanoparticles and glucose oxidase: Physicochemical characterization of the platform and evaluation of its biocatalytic activity
title_full_unstemmed	Quaternized chitosan as support for the assembly of gold nanoparticles and glucose oxidase: Physicochemical characterization of the platform and evaluation of its biocatalytic activity
title_sort	Quaternized chitosan as support for the assembly of gold nanoparticles and glucose oxidase: Physicochemical characterization of the platform and evaluation of its biocatalytic activity
dc.creator.none.fl_str_mv	Bracamonte, Maria Victoria Bollo, Soledad Labbé, Pierre Rivas, Gustavo Adolfo Ferreyra, Nancy Fabiana
author	Bracamonte, Maria Victoria
author_facet	Bracamonte, Maria Victoria Bollo, Soledad Labbé, Pierre Rivas, Gustavo Adolfo Ferreyra, Nancy Fabiana
author_role	author
author2	Bollo, Soledad Labbé, Pierre Rivas, Gustavo Adolfo Ferreyra, Nancy Fabiana
author2_role	author author author author
dc.subject.none.fl_str_mv	Glucose Oxidase Gold Nanoparticles Quaternized Chitosan Scanning Electrochemical Microscopy Surface Plasmon Resonance
topic	Glucose Oxidase Gold Nanoparticles Quaternized Chitosan Scanning Electrochemical Microscopy Surface Plasmon Resonance
purl_subject.fl_str_mv	https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv	We report for the first time the use of quaternized chitosan (QCHI) for the immobilization of gold nanoparticles (NP) and glucose oxidase (GOD), the characterization of the resulting platform and its biocatalytic activity using glucose as substrate. The chemical substitution of chitosan has allowed us to work at physiologic pH to build up self-assembled layers of QCHI-NP as platform for the enzyme immobilization. The adsorption of GOD was analyzed by surface plasmon resonance (SPR) to compare the surface coverage of GOD in absence and presence of the QCHI-NP platform. The results obtained with cyclic voltammetry and scanning electrochemical microscopy (SECM) revealed that the adsorption of NP improves the conductivity of the structure and its electrochemical reactivity, facilitating the oxidation of the hydrogen peroxide produced by GOD. The electrodes modified with NP present higher amperometric response demonstrating the efficient transduction of the enzymatic activity in this structure. © 2010 Elsevier Ltd. All rights reserved. Fil: Bracamonte, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina Fil: Bollo, Soledad. Universidad de Chile; Chile Fil: Labbé, Pierre. Universite Joseph Fourier; Francia Fil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina Fil: Ferreyra, Nancy Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
description	We report for the first time the use of quaternized chitosan (QCHI) for the immobilization of gold nanoparticles (NP) and glucose oxidase (GOD), the characterization of the resulting platform and its biocatalytic activity using glucose as substrate. The chemical substitution of chitosan has allowed us to work at physiologic pH to build up self-assembled layers of QCHI-NP as platform for the enzyme immobilization. The adsorption of GOD was analyzed by surface plasmon resonance (SPR) to compare the surface coverage of GOD in absence and presence of the QCHI-NP platform. The results obtained with cyclic voltammetry and scanning electrochemical microscopy (SECM) revealed that the adsorption of NP improves the conductivity of the structure and its electrochemical reactivity, facilitating the oxidation of the hydrogen peroxide produced by GOD. The electrodes modified with NP present higher amperometric response demonstrating the efficient transduction of the enzymatic activity in this structure. © 2010 Elsevier Ltd. All rights reserved.
publishDate	2011
dc.date.none.fl_str_mv	2011-01
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/61612 Bracamonte, Maria Victoria; Bollo, Soledad; Labbé, Pierre; Rivas, Gustavo Adolfo; Ferreyra, Nancy Fabiana; Quaternized chitosan as support for the assembly of gold nanoparticles and glucose oxidase: Physicochemical characterization of the platform and evaluation of its biocatalytic activity; Pergamon-Elsevier Science Ltd; Electrochimica Acta; 56; 3; 1-2011; 1316-1322 0013-4686 CONICET Digital CONICET
url	http://hdl.handle.net/11336/61612
identifier_str_mv	Bracamonte, Maria Victoria; Bollo, Soledad; Labbé, Pierre; Rivas, Gustavo Adolfo; Ferreyra, Nancy Fabiana; Quaternized chitosan as support for the assembly of gold nanoparticles and glucose oxidase: Physicochemical characterization of the platform and evaluation of its biocatalytic activity; Pergamon-Elsevier Science Ltd; Electrochimica Acta; 56; 3; 1-2011; 1316-1322 0013-4686 CONICET Digital CONICET
dc.language.none.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	info:eu-repo/semantics/altIdentifier/doi/10.1016/j.electacta.2010.10.022 info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0013468610013885
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv	openAccess
rights_invalid_str_mv	https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv	application/pdf application/pdf application/pdf application/pdf
dc.publisher.none.fl_str_mv	Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv	Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv	reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str	CONICET Digital (CONICET)
collection	CONICET Digital (CONICET)
instname_str	Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv	CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv	dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score	13.22299

Quaternized chitosan as support for the assembly of gold nanoparticles and glucose oxidase: Physicochemical characterization of the platform and evaluation of its biocatalytic acti...

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