Expanding the Benefits of Tnt1 for the Identification of Dominant Mutations in Polyploid Crops: A Single Allelic Mutation in the MsNAC39 Gene Produces Multifoliated Alfalfa

Autores
Jozefkowicz, Cintia; Gómez, Cristina; Odorizzi, Ariel Sebastian; Iantcheva, Anelia; Ratet, Pascal; Ayub, Nicolás; Soto, Gabriela Cynthia
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Most major crops are polyploid species and the production of genetically engineered cultivars normally requires the introgression of transgenic or gene-edited traits into elite germplasm. Thus, a main goal of plant research is the search of systems to identify dominant mutations. In this article, we show that the Tnt1 element can be used to identify dominant mutations in allogamous tetraploid cultivated alfalfa. Specifically, we show that a single allelic mutation in the MsNAC39 gene produces multifoliate leaves (mfl) alfalfa plants, a pivot trait of breeding programs of this forage species. Finally, we discuss the potential application of a combination of preliminary screening of beneficial dominant mutants using Tnt1 mutant libraries and genome editing via the CRISPR/Cas9 system to identify target genes and to rapidly improve both autogamous and allogamous polyploid crops.
Fil: Jozefkowicz, Cintia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Gómez, Cristina. Agencia de Extension Rural Villa Maria (aer Villa Maria) ; Estacion Experimental Agropecuaria Manfredi ; Centro Regional Cordoba ; Instituto Nacional de Tecnologia Agropecuaria;
Fil: Odorizzi, Ariel Sebastian. Agencia de Extension Rural Villa Maria (aer Villa Maria) ; Estacion Experimental Agropecuaria Manfredi ; Centro Regional Cordoba ; Instituto Nacional de Tecnologia Agropecuaria;
Fil: Iantcheva, Anelia. AgroBioInstitute Agricultural Academy; Bulgaria
Fil: Ratet, Pascal. Université D'evry Val D'essonne; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Ayub, Nicolás. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Soto, Gabriela Cynthia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Materia
ALFALFA
DOMINANT MUTATION
POLYPLOIDY
TNT1
TRANSFORMATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/182277
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Expanding the Benefits of Tnt1 for the Identification of Dominant Mutations in Polyploid Crops: A Single Allelic Mutation in the MsNAC39 Gene Produces Multifoliated AlfalfaJozefkowicz, CintiaGómez, CristinaOdorizzi, Ariel SebastianIantcheva, AneliaRatet, PascalAyub, NicolásSoto, Gabriela CynthiaALFALFADOMINANT MUTATIONPOLYPLOIDYTNT1TRANSFORMATIONhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Most major crops are polyploid species and the production of genetically engineered cultivars normally requires the introgression of transgenic or gene-edited traits into elite germplasm. Thus, a main goal of plant research is the search of systems to identify dominant mutations. In this article, we show that the Tnt1 element can be used to identify dominant mutations in allogamous tetraploid cultivated alfalfa. Specifically, we show that a single allelic mutation in the MsNAC39 gene produces multifoliate leaves (mfl) alfalfa plants, a pivot trait of breeding programs of this forage species. Finally, we discuss the potential application of a combination of preliminary screening of beneficial dominant mutants using Tnt1 mutant libraries and genome editing via the CRISPR/Cas9 system to identify target genes and to rapidly improve both autogamous and allogamous polyploid crops.Fil: Jozefkowicz, Cintia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Gómez, Cristina. Agencia de Extension Rural Villa Maria (aer Villa Maria) ; Estacion Experimental Agropecuaria Manfredi ; Centro Regional Cordoba ; Instituto Nacional de Tecnologia Agropecuaria;Fil: Odorizzi, Ariel Sebastian. Agencia de Extension Rural Villa Maria (aer Villa Maria) ; Estacion Experimental Agropecuaria Manfredi ; Centro Regional Cordoba ; Instituto Nacional de Tecnologia Agropecuaria;Fil: Iantcheva, Anelia. AgroBioInstitute Agricultural Academy; BulgariaFil: Ratet, Pascal. Université D'evry Val D'essonne; Francia. Centre National de la Recherche Scientifique; FranciaFil: Ayub, Nicolás. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; ArgentinaFil: Soto, Gabriela Cynthia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFrontiers Media2021-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/182277Jozefkowicz, Cintia; Gómez, Cristina; Odorizzi, Ariel Sebastian; Iantcheva, Anelia; Ratet, Pascal; et al.; Expanding the Benefits of Tnt1 for the Identification of Dominant Mutations in Polyploid Crops: A Single Allelic Mutation in the MsNAC39 Gene Produces Multifoliated Alfalfa; Frontiers Media; Frontiers in Plant Science; 12; 805032; 12-2021; 1-61664-462XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fpls.2021.805032/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2021.805032info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:09:51Zoai:ri.conicet.gov.ar:11336/182277instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:09:51.783CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Expanding the Benefits of Tnt1 for the Identification of Dominant Mutations in Polyploid Crops: A Single Allelic Mutation in the MsNAC39 Gene Produces Multifoliated Alfalfa
title Expanding the Benefits of Tnt1 for the Identification of Dominant Mutations in Polyploid Crops: A Single Allelic Mutation in the MsNAC39 Gene Produces Multifoliated Alfalfa
spellingShingle Expanding the Benefits of Tnt1 for the Identification of Dominant Mutations in Polyploid Crops: A Single Allelic Mutation in the MsNAC39 Gene Produces Multifoliated Alfalfa
Jozefkowicz, Cintia
ALFALFA
DOMINANT MUTATION
POLYPLOIDY
TNT1
TRANSFORMATION
title_short Expanding the Benefits of Tnt1 for the Identification of Dominant Mutations in Polyploid Crops: A Single Allelic Mutation in the MsNAC39 Gene Produces Multifoliated Alfalfa
title_full Expanding the Benefits of Tnt1 for the Identification of Dominant Mutations in Polyploid Crops: A Single Allelic Mutation in the MsNAC39 Gene Produces Multifoliated Alfalfa
title_fullStr Expanding the Benefits of Tnt1 for the Identification of Dominant Mutations in Polyploid Crops: A Single Allelic Mutation in the MsNAC39 Gene Produces Multifoliated Alfalfa
title_full_unstemmed Expanding the Benefits of Tnt1 for the Identification of Dominant Mutations in Polyploid Crops: A Single Allelic Mutation in the MsNAC39 Gene Produces Multifoliated Alfalfa
title_sort Expanding the Benefits of Tnt1 for the Identification of Dominant Mutations in Polyploid Crops: A Single Allelic Mutation in the MsNAC39 Gene Produces Multifoliated Alfalfa
dc.creator.none.fl_str_mv Jozefkowicz, Cintia
Gómez, Cristina
Odorizzi, Ariel Sebastian
Iantcheva, Anelia
Ratet, Pascal
Ayub, Nicolás
Soto, Gabriela Cynthia
author Jozefkowicz, Cintia
author_facet Jozefkowicz, Cintia
Gómez, Cristina
Odorizzi, Ariel Sebastian
Iantcheva, Anelia
Ratet, Pascal
Ayub, Nicolás
Soto, Gabriela Cynthia
author_role author
author2 Gómez, Cristina
Odorizzi, Ariel Sebastian
Iantcheva, Anelia
Ratet, Pascal
Ayub, Nicolás
Soto, Gabriela Cynthia
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ALFALFA
DOMINANT MUTATION
POLYPLOIDY
TNT1
TRANSFORMATION
topic ALFALFA
DOMINANT MUTATION
POLYPLOIDY
TNT1
TRANSFORMATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Most major crops are polyploid species and the production of genetically engineered cultivars normally requires the introgression of transgenic or gene-edited traits into elite germplasm. Thus, a main goal of plant research is the search of systems to identify dominant mutations. In this article, we show that the Tnt1 element can be used to identify dominant mutations in allogamous tetraploid cultivated alfalfa. Specifically, we show that a single allelic mutation in the MsNAC39 gene produces multifoliate leaves (mfl) alfalfa plants, a pivot trait of breeding programs of this forage species. Finally, we discuss the potential application of a combination of preliminary screening of beneficial dominant mutants using Tnt1 mutant libraries and genome editing via the CRISPR/Cas9 system to identify target genes and to rapidly improve both autogamous and allogamous polyploid crops.
Fil: Jozefkowicz, Cintia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Gómez, Cristina. Agencia de Extension Rural Villa Maria (aer Villa Maria) ; Estacion Experimental Agropecuaria Manfredi ; Centro Regional Cordoba ; Instituto Nacional de Tecnologia Agropecuaria;
Fil: Odorizzi, Ariel Sebastian. Agencia de Extension Rural Villa Maria (aer Villa Maria) ; Estacion Experimental Agropecuaria Manfredi ; Centro Regional Cordoba ; Instituto Nacional de Tecnologia Agropecuaria;
Fil: Iantcheva, Anelia. AgroBioInstitute Agricultural Academy; Bulgaria
Fil: Ratet, Pascal. Université D'evry Val D'essonne; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Ayub, Nicolás. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Soto, Gabriela Cynthia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
description Most major crops are polyploid species and the production of genetically engineered cultivars normally requires the introgression of transgenic or gene-edited traits into elite germplasm. Thus, a main goal of plant research is the search of systems to identify dominant mutations. In this article, we show that the Tnt1 element can be used to identify dominant mutations in allogamous tetraploid cultivated alfalfa. Specifically, we show that a single allelic mutation in the MsNAC39 gene produces multifoliate leaves (mfl) alfalfa plants, a pivot trait of breeding programs of this forage species. Finally, we discuss the potential application of a combination of preliminary screening of beneficial dominant mutants using Tnt1 mutant libraries and genome editing via the CRISPR/Cas9 system to identify target genes and to rapidly improve both autogamous and allogamous polyploid crops.
publishDate 2021
dc.date.none.fl_str_mv 2021-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/182277
Jozefkowicz, Cintia; Gómez, Cristina; Odorizzi, Ariel Sebastian; Iantcheva, Anelia; Ratet, Pascal; et al.; Expanding the Benefits of Tnt1 for the Identification of Dominant Mutations in Polyploid Crops: A Single Allelic Mutation in the MsNAC39 Gene Produces Multifoliated Alfalfa; Frontiers Media; Frontiers in Plant Science; 12; 805032; 12-2021; 1-6
1664-462X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/182277
identifier_str_mv Jozefkowicz, Cintia; Gómez, Cristina; Odorizzi, Ariel Sebastian; Iantcheva, Anelia; Ratet, Pascal; et al.; Expanding the Benefits of Tnt1 for the Identification of Dominant Mutations in Polyploid Crops: A Single Allelic Mutation in the MsNAC39 Gene Produces Multifoliated Alfalfa; Frontiers Media; Frontiers in Plant Science; 12; 805032; 12-2021; 1-6
1664-462X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fpls.2021.805032/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2021.805032
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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