Variation of rDNA loci of polyploid Solanum elaeagnifolium (Solanaceae)

Autores
Chiarini, Franco Ezequiel
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fluorescence in situ hybridization (FISH) was applied to detect gene rearrangements and genome downsizing in Solanum elaeagnifolium, an invasive weed with 2n = 24, 48 and 72 populations. The probes employed were pTa71 containing the 18S5.8S26S gene, and a 5S rDNA probe obtained from S. stuckertii. As a result, diploids presented a pair of 18S5.8S26S signals and a pair of 5S, whereas tetra- and hexaploids increased their FISH marks proportional to the ploidy level. In all cases, rDNA loci were non-syntenic, and position was conserved along the polyploid series. The cytotypes would have formed recently and drastic rearrangements have not yet occurred. However, in each cell of the 4x and 6x individuals, signal intensity in one locus of the 18S5.8S26S was stronger, suggesting a potential loss of copies in the remaining loci. In addition, one hexaploid showed an extra 5S signal in heterozygosis. The data obtained suggest a recent, recurrent autopolyploidy.
Fil: Chiarini, Franco Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina
Materia
Solanum Elaeagnifolium
Polyploidy
Rdna Loci
Genome Downsizing
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/9318
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/9318
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Variation of rDNA loci of polyploid Solanum elaeagnifolium (Solanaceae)Chiarini, Franco EzequielSolanum ElaeagnifoliumPolyploidyRdna LociGenome Downsizinghttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Fluorescence in situ hybridization (FISH) was applied to detect gene rearrangements and genome downsizing in Solanum elaeagnifolium, an invasive weed with 2n = 24, 48 and 72 populations. The probes employed were pTa71 containing the 18S5.8S26S gene, and a 5S rDNA probe obtained from S. stuckertii. As a result, diploids presented a pair of 18S5.8S26S signals and a pair of 5S, whereas tetra- and hexaploids increased their FISH marks proportional to the ploidy level. In all cases, rDNA loci were non-syntenic, and position was conserved along the polyploid series. The cytotypes would have formed recently and drastic rearrangements have not yet occurred. However, in each cell of the 4x and 6x individuals, signal intensity in one locus of the 18S5.8S26S was stronger, suggesting a potential loss of copies in the remaining loci. In addition, one hexaploid showed an extra 5S signal in heterozygosis. The data obtained suggest a recent, recurrent autopolyploidy.Fil: Chiarini, Franco Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); ArgentinaRsnz Publishing2014-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/9318Chiarini, Franco Ezequiel; Variation of rDNA loci of polyploid Solanum elaeagnifolium (Solanaceae); Rsnz Publishing; New Zealand Journal Of Botany; 52; 3; 5-2014; 277-2840028-825Xenginfo:eu-repo/semantics/altIdentifier/doi/10.1080/0028825X.2014.888087info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.1080/0028825X.2014.888087info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:28Zoai:ri.conicet.gov.ar:11336/9318instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:29.353CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Variation of rDNA loci of polyploid Solanum elaeagnifolium (Solanaceae)
title Variation of rDNA loci of polyploid Solanum elaeagnifolium (Solanaceae)
spellingShingle Variation of rDNA loci of polyploid Solanum elaeagnifolium (Solanaceae)
Chiarini, Franco Ezequiel
Solanum Elaeagnifolium
Polyploidy
Rdna Loci
Genome Downsizing
title_short Variation of rDNA loci of polyploid Solanum elaeagnifolium (Solanaceae)
title_full Variation of rDNA loci of polyploid Solanum elaeagnifolium (Solanaceae)
title_fullStr Variation of rDNA loci of polyploid Solanum elaeagnifolium (Solanaceae)
title_full_unstemmed Variation of rDNA loci of polyploid Solanum elaeagnifolium (Solanaceae)
title_sort Variation of rDNA loci of polyploid Solanum elaeagnifolium (Solanaceae)
dc.creator.none.fl_str_mv Chiarini, Franco Ezequiel
author Chiarini, Franco Ezequiel
author_facet Chiarini, Franco Ezequiel
author_role author
dc.subject.none.fl_str_mv Solanum Elaeagnifolium
Polyploidy
Rdna Loci
Genome Downsizing
topic Solanum Elaeagnifolium
Polyploidy
Rdna Loci
Genome Downsizing
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Fluorescence in situ hybridization (FISH) was applied to detect gene rearrangements and genome downsizing in Solanum elaeagnifolium, an invasive weed with 2n = 24, 48 and 72 populations. The probes employed were pTa71 containing the 18S5.8S26S gene, and a 5S rDNA probe obtained from S. stuckertii. As a result, diploids presented a pair of 18S5.8S26S signals and a pair of 5S, whereas tetra- and hexaploids increased their FISH marks proportional to the ploidy level. In all cases, rDNA loci were non-syntenic, and position was conserved along the polyploid series. The cytotypes would have formed recently and drastic rearrangements have not yet occurred. However, in each cell of the 4x and 6x individuals, signal intensity in one locus of the 18S5.8S26S was stronger, suggesting a potential loss of copies in the remaining loci. In addition, one hexaploid showed an extra 5S signal in heterozygosis. The data obtained suggest a recent, recurrent autopolyploidy.
Fil: Chiarini, Franco Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina
description Fluorescence in situ hybridization (FISH) was applied to detect gene rearrangements and genome downsizing in Solanum elaeagnifolium, an invasive weed with 2n = 24, 48 and 72 populations. The probes employed were pTa71 containing the 18S5.8S26S gene, and a 5S rDNA probe obtained from S. stuckertii. As a result, diploids presented a pair of 18S5.8S26S signals and a pair of 5S, whereas tetra- and hexaploids increased their FISH marks proportional to the ploidy level. In all cases, rDNA loci were non-syntenic, and position was conserved along the polyploid series. The cytotypes would have formed recently and drastic rearrangements have not yet occurred. However, in each cell of the 4x and 6x individuals, signal intensity in one locus of the 18S5.8S26S was stronger, suggesting a potential loss of copies in the remaining loci. In addition, one hexaploid showed an extra 5S signal in heterozygosis. The data obtained suggest a recent, recurrent autopolyploidy.
publishDate 2014
dc.date.none.fl_str_mv 2014-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/9318
Chiarini, Franco Ezequiel; Variation of rDNA loci of polyploid Solanum elaeagnifolium (Solanaceae); Rsnz Publishing; New Zealand Journal Of Botany; 52; 3; 5-2014; 277-284
0028-825X
url http://hdl.handle.net/11336/9318
identifier_str_mv Chiarini, Franco Ezequiel; Variation of rDNA loci of polyploid Solanum elaeagnifolium (Solanaceae); Rsnz Publishing; New Zealand Journal Of Botany; 52; 3; 5-2014; 277-284
0028-825X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1080/0028825X.2014.888087
info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.1080/0028825X.2014.888087
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Rsnz Publishing
publisher.none.fl_str_mv Rsnz Publishing
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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