Agonist and cholesterol modulate the alpha7 acetylcholine receptor in non-neural endothelial cells

Autores
Ayala Peña, Victoria Belen; Bonini, Ida Clara; Barrantes, Francisco Jose
Año de publicación
2010
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The "neuronal" α7-type nicotinic acetylcholine receptor (α7AChR) is found in various non-neural tissues, including vascular 2+ endothelium, where its peculiar ionotropic (high Ca permeability) 2+ and metabotropic (Ca -mediated intracellular cascades) properties may play important roles in angiogenesis, inflammation and atherogenesis. Molecular properties of the α7AChR, its response to nicotine stimulation, and its cellular distribution were studied here using a combination of pharmacological, biochemical and fluorescence microscopy tools. α7AChRs in rat arterial endothelial cells (RAEC) were found to undergo agonist (nicotine)-induced upregulation (from 53±16 to 385.2±46.8 fmol/mg protein with 50 µM nicotine), increasing their cell-surface exposure. α7AChRs occurred predominantly in the “non-raft" subcellular fractions, yet cholesterol depletion mediated by cyclodextrin treatment reduced the number of cell-surface α7AChRs. Nicotine was found to increase the affinity of the α7AChR for crystal violet, an openchannel blocker. Under basal conditions, α7AChRs in endothelial cells displayed a high-affinity, presumably desensitized conformation (Kd ~0.76 nM), and both nicotine and cyclodextrin affected their cell-surface expression.
Fil: Ayala Peña, Victoria Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Unesco; Argentina
Fil: Bonini, Ida Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Unesco; Argentina
Fil: Barrantes, Francisco Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Unesco; Argentina
XLVI Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Puerto Madryn
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Materia
alpha-7 Acetylcholine Receptor
Endothelial cells
Cholesterol
Agonist
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/222692
Acceder
id CONICETDig_ae62cb2fc362e103723588e93fb4c6a2
oai_identifier_str oai:ri.conicet.gov.ar:11336/222692
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Agonist and cholesterol modulate the alpha7 acetylcholine receptor in non-neural endothelial cellsAyala Peña, Victoria BelenBonini, Ida ClaraBarrantes, Francisco Josealpha-7 Acetylcholine ReceptorEndothelial cellsCholesterolAgonisthttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The "neuronal" α7-type nicotinic acetylcholine receptor (α7AChR) is found in various non-neural tissues, including vascular 2+ endothelium, where its peculiar ionotropic (high Ca permeability) 2+ and metabotropic (Ca -mediated intracellular cascades) properties may play important roles in angiogenesis, inflammation and atherogenesis. Molecular properties of the α7AChR, its response to nicotine stimulation, and its cellular distribution were studied here using a combination of pharmacological, biochemical and fluorescence microscopy tools. α7AChRs in rat arterial endothelial cells (RAEC) were found to undergo agonist (nicotine)-induced upregulation (from 53±16 to 385.2±46.8 fmol/mg protein with 50 µM nicotine), increasing their cell-surface exposure. α7AChRs occurred predominantly in the “non-raft" subcellular fractions, yet cholesterol depletion mediated by cyclodextrin treatment reduced the number of cell-surface α7AChRs. Nicotine was found to increase the affinity of the α7AChR for crystal violet, an openchannel blocker. Under basal conditions, α7AChRs in endothelial cells displayed a high-affinity, presumably desensitized conformation (Kd ~0.76 nM), and both nicotine and cyclodextrin affected their cell-surface expression.Fil: Ayala Peña, Victoria Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Unesco; ArgentinaFil: Bonini, Ida Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Unesco; ArgentinaFil: Barrantes, Francisco Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Unesco; ArgentinaXLVI Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología MolecularPuerto MadrynArgentinaSociedad Argentina de Investigación Bioquímica y Biología MolecularBiocell2010info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/222692Agonist and cholesterol modulate the alpha7 acetylcholine receptor in non-neural endothelial cells; XLVI Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular; Puerto Madryn; Argentina; 2010; 123-1230327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/biocell-34.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:06:14Zoai:ri.conicet.gov.ar:11336/222692instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:06:14.793CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Agonist and cholesterol modulate the alpha7 acetylcholine receptor in non-neural endothelial cells
title Agonist and cholesterol modulate the alpha7 acetylcholine receptor in non-neural endothelial cells
spellingShingle Agonist and cholesterol modulate the alpha7 acetylcholine receptor in non-neural endothelial cells
Ayala Peña, Victoria Belen
alpha-7 Acetylcholine Receptor
Endothelial cells
Cholesterol
Agonist
title_short Agonist and cholesterol modulate the alpha7 acetylcholine receptor in non-neural endothelial cells
title_full Agonist and cholesterol modulate the alpha7 acetylcholine receptor in non-neural endothelial cells
title_fullStr Agonist and cholesterol modulate the alpha7 acetylcholine receptor in non-neural endothelial cells
title_full_unstemmed Agonist and cholesterol modulate the alpha7 acetylcholine receptor in non-neural endothelial cells
title_sort Agonist and cholesterol modulate the alpha7 acetylcholine receptor in non-neural endothelial cells
dc.creator.none.fl_str_mv Ayala Peña, Victoria Belen
Bonini, Ida Clara
Barrantes, Francisco Jose
author Ayala Peña, Victoria Belen
author_facet Ayala Peña, Victoria Belen
Bonini, Ida Clara
Barrantes, Francisco Jose
author_role author
author2 Bonini, Ida Clara
Barrantes, Francisco Jose
author2_role author
author
dc.subject.none.fl_str_mv alpha-7 Acetylcholine Receptor
Endothelial cells
Cholesterol
Agonist
topic alpha-7 Acetylcholine Receptor
Endothelial cells
Cholesterol
Agonist
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The "neuronal" α7-type nicotinic acetylcholine receptor (α7AChR) is found in various non-neural tissues, including vascular 2+ endothelium, where its peculiar ionotropic (high Ca permeability) 2+ and metabotropic (Ca -mediated intracellular cascades) properties may play important roles in angiogenesis, inflammation and atherogenesis. Molecular properties of the α7AChR, its response to nicotine stimulation, and its cellular distribution were studied here using a combination of pharmacological, biochemical and fluorescence microscopy tools. α7AChRs in rat arterial endothelial cells (RAEC) were found to undergo agonist (nicotine)-induced upregulation (from 53±16 to 385.2±46.8 fmol/mg protein with 50 µM nicotine), increasing their cell-surface exposure. α7AChRs occurred predominantly in the “non-raft" subcellular fractions, yet cholesterol depletion mediated by cyclodextrin treatment reduced the number of cell-surface α7AChRs. Nicotine was found to increase the affinity of the α7AChR for crystal violet, an openchannel blocker. Under basal conditions, α7AChRs in endothelial cells displayed a high-affinity, presumably desensitized conformation (Kd ~0.76 nM), and both nicotine and cyclodextrin affected their cell-surface expression.
Fil: Ayala Peña, Victoria Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Unesco; Argentina
Fil: Bonini, Ida Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Unesco; Argentina
Fil: Barrantes, Francisco Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Unesco; Argentina
XLVI Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Puerto Madryn
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
description The "neuronal" α7-type nicotinic acetylcholine receptor (α7AChR) is found in various non-neural tissues, including vascular 2+ endothelium, where its peculiar ionotropic (high Ca permeability) 2+ and metabotropic (Ca -mediated intracellular cascades) properties may play important roles in angiogenesis, inflammation and atherogenesis. Molecular properties of the α7AChR, its response to nicotine stimulation, and its cellular distribution were studied here using a combination of pharmacological, biochemical and fluorescence microscopy tools. α7AChRs in rat arterial endothelial cells (RAEC) were found to undergo agonist (nicotine)-induced upregulation (from 53±16 to 385.2±46.8 fmol/mg protein with 50 µM nicotine), increasing their cell-surface exposure. α7AChRs occurred predominantly in the “non-raft" subcellular fractions, yet cholesterol depletion mediated by cyclodextrin treatment reduced the number of cell-surface α7AChRs. Nicotine was found to increase the affinity of the α7AChR for crystal violet, an openchannel blocker. Under basal conditions, α7AChRs in endothelial cells displayed a high-affinity, presumably desensitized conformation (Kd ~0.76 nM), and both nicotine and cyclodextrin affected their cell-surface expression.
publishDate 2010
dc.date.none.fl_str_mv 2010
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/222692
Agonist and cholesterol modulate the alpha7 acetylcholine receptor in non-neural endothelial cells; XLVI Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular; Puerto Madryn; Argentina; 2010; 123-123
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/222692
identifier_str_mv Agonist and cholesterol modulate the alpha7 acetylcholine receptor in non-neural endothelial cells; XLVI Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular; Puerto Madryn; Argentina; 2010; 123-123
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/biocell-34.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Biocell
publisher.none.fl_str_mv Biocell
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782418239881216
score 12.982451

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