Agonist and cholesterol modulate the alpha7 acetylcholine receptor in non-neural endothelial cells
- Autores
- Ayala Peña, Victoria Belen; Bonini, Ida Clara; Barrantes, Francisco Jose
- Año de publicación
- 2010
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- The "neuronal" α7-type nicotinic acetylcholine receptor (α7AChR) is found in various non-neural tissues, including vascular 2+ endothelium, where its peculiar ionotropic (high Ca permeability) 2+ and metabotropic (Ca -mediated intracellular cascades) properties may play important roles in angiogenesis, inflammation and atherogenesis. Molecular properties of the α7AChR, its response to nicotine stimulation, and its cellular distribution were studied here using a combination of pharmacological, biochemical and fluorescence microscopy tools. α7AChRs in rat arterial endothelial cells (RAEC) were found to undergo agonist (nicotine)-induced upregulation (from 53±16 to 385.2±46.8 fmol/mg protein with 50 µM nicotine), increasing their cell-surface exposure. α7AChRs occurred predominantly in the “non-raft" subcellular fractions, yet cholesterol depletion mediated by cyclodextrin treatment reduced the number of cell-surface α7AChRs. Nicotine was found to increase the affinity of the α7AChR for crystal violet, an openchannel blocker. Under basal conditions, α7AChRs in endothelial cells displayed a high-affinity, presumably desensitized conformation (Kd ~0.76 nM), and both nicotine and cyclodextrin affected their cell-surface expression.
Fil: Ayala Peña, Victoria Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Unesco; Argentina
Fil: Bonini, Ida Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Unesco; Argentina
Fil: Barrantes, Francisco Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Unesco; Argentina
XLVI Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Puerto Madryn
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología Molecular - Materia
-
alpha-7 Acetylcholine Receptor
Endothelial cells
Cholesterol
Agonist - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/222692
Ver los metadatos del registro completo
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Agonist and cholesterol modulate the alpha7 acetylcholine receptor in non-neural endothelial cellsAyala Peña, Victoria BelenBonini, Ida ClaraBarrantes, Francisco Josealpha-7 Acetylcholine ReceptorEndothelial cellsCholesterolAgonisthttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The "neuronal" α7-type nicotinic acetylcholine receptor (α7AChR) is found in various non-neural tissues, including vascular 2+ endothelium, where its peculiar ionotropic (high Ca permeability) 2+ and metabotropic (Ca -mediated intracellular cascades) properties may play important roles in angiogenesis, inflammation and atherogenesis. Molecular properties of the α7AChR, its response to nicotine stimulation, and its cellular distribution were studied here using a combination of pharmacological, biochemical and fluorescence microscopy tools. α7AChRs in rat arterial endothelial cells (RAEC) were found to undergo agonist (nicotine)-induced upregulation (from 53±16 to 385.2±46.8 fmol/mg protein with 50 µM nicotine), increasing their cell-surface exposure. α7AChRs occurred predominantly in the “non-raft" subcellular fractions, yet cholesterol depletion mediated by cyclodextrin treatment reduced the number of cell-surface α7AChRs. Nicotine was found to increase the affinity of the α7AChR for crystal violet, an openchannel blocker. Under basal conditions, α7AChRs in endothelial cells displayed a high-affinity, presumably desensitized conformation (Kd ~0.76 nM), and both nicotine and cyclodextrin affected their cell-surface expression.Fil: Ayala Peña, Victoria Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Unesco; ArgentinaFil: Bonini, Ida Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Unesco; ArgentinaFil: Barrantes, Francisco Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Unesco; ArgentinaXLVI Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología MolecularPuerto MadrynArgentinaSociedad Argentina de Investigación Bioquímica y Biología MolecularBiocell2010info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/222692Agonist and cholesterol modulate the alpha7 acetylcholine receptor in non-neural endothelial cells; XLVI Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular; Puerto Madryn; Argentina; 2010; 123-1230327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/biocell-34.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:33:43Zoai:ri.conicet.gov.ar:11336/222692instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:33:43.369CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Agonist and cholesterol modulate the alpha7 acetylcholine receptor in non-neural endothelial cells |
title |
Agonist and cholesterol modulate the alpha7 acetylcholine receptor in non-neural endothelial cells |
spellingShingle |
Agonist and cholesterol modulate the alpha7 acetylcholine receptor in non-neural endothelial cells Ayala Peña, Victoria Belen alpha-7 Acetylcholine Receptor Endothelial cells Cholesterol Agonist |
title_short |
Agonist and cholesterol modulate the alpha7 acetylcholine receptor in non-neural endothelial cells |
title_full |
Agonist and cholesterol modulate the alpha7 acetylcholine receptor in non-neural endothelial cells |
title_fullStr |
Agonist and cholesterol modulate the alpha7 acetylcholine receptor in non-neural endothelial cells |
title_full_unstemmed |
Agonist and cholesterol modulate the alpha7 acetylcholine receptor in non-neural endothelial cells |
title_sort |
Agonist and cholesterol modulate the alpha7 acetylcholine receptor in non-neural endothelial cells |
dc.creator.none.fl_str_mv |
Ayala Peña, Victoria Belen Bonini, Ida Clara Barrantes, Francisco Jose |
author |
Ayala Peña, Victoria Belen |
author_facet |
Ayala Peña, Victoria Belen Bonini, Ida Clara Barrantes, Francisco Jose |
author_role |
author |
author2 |
Bonini, Ida Clara Barrantes, Francisco Jose |
author2_role |
author author |
dc.subject.none.fl_str_mv |
alpha-7 Acetylcholine Receptor Endothelial cells Cholesterol Agonist |
topic |
alpha-7 Acetylcholine Receptor Endothelial cells Cholesterol Agonist |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The "neuronal" α7-type nicotinic acetylcholine receptor (α7AChR) is found in various non-neural tissues, including vascular 2+ endothelium, where its peculiar ionotropic (high Ca permeability) 2+ and metabotropic (Ca -mediated intracellular cascades) properties may play important roles in angiogenesis, inflammation and atherogenesis. Molecular properties of the α7AChR, its response to nicotine stimulation, and its cellular distribution were studied here using a combination of pharmacological, biochemical and fluorescence microscopy tools. α7AChRs in rat arterial endothelial cells (RAEC) were found to undergo agonist (nicotine)-induced upregulation (from 53±16 to 385.2±46.8 fmol/mg protein with 50 µM nicotine), increasing their cell-surface exposure. α7AChRs occurred predominantly in the “non-raft" subcellular fractions, yet cholesterol depletion mediated by cyclodextrin treatment reduced the number of cell-surface α7AChRs. Nicotine was found to increase the affinity of the α7AChR for crystal violet, an openchannel blocker. Under basal conditions, α7AChRs in endothelial cells displayed a high-affinity, presumably desensitized conformation (Kd ~0.76 nM), and both nicotine and cyclodextrin affected their cell-surface expression. Fil: Ayala Peña, Victoria Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Unesco; Argentina Fil: Bonini, Ida Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Unesco; Argentina Fil: Barrantes, Francisco Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Unesco; Argentina XLVI Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular Puerto Madryn Argentina Sociedad Argentina de Investigación Bioquímica y Biología Molecular |
description |
The "neuronal" α7-type nicotinic acetylcholine receptor (α7AChR) is found in various non-neural tissues, including vascular 2+ endothelium, where its peculiar ionotropic (high Ca permeability) 2+ and metabotropic (Ca -mediated intracellular cascades) properties may play important roles in angiogenesis, inflammation and atherogenesis. Molecular properties of the α7AChR, its response to nicotine stimulation, and its cellular distribution were studied here using a combination of pharmacological, biochemical and fluorescence microscopy tools. α7AChRs in rat arterial endothelial cells (RAEC) were found to undergo agonist (nicotine)-induced upregulation (from 53±16 to 385.2±46.8 fmol/mg protein with 50 µM nicotine), increasing their cell-surface exposure. α7AChRs occurred predominantly in the “non-raft" subcellular fractions, yet cholesterol depletion mediated by cyclodextrin treatment reduced the number of cell-surface α7AChRs. Nicotine was found to increase the affinity of the α7AChR for crystal violet, an openchannel blocker. Under basal conditions, α7AChRs in endothelial cells displayed a high-affinity, presumably desensitized conformation (Kd ~0.76 nM), and both nicotine and cyclodextrin affected their cell-surface expression. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Congreso Journal http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
status_str |
publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/222692 Agonist and cholesterol modulate the alpha7 acetylcholine receptor in non-neural endothelial cells; XLVI Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular; Puerto Madryn; Argentina; 2010; 123-123 0327-9545 1667-5746 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/222692 |
identifier_str_mv |
Agonist and cholesterol modulate the alpha7 acetylcholine receptor in non-neural endothelial cells; XLVI Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular; Puerto Madryn; Argentina; 2010; 123-123 0327-9545 1667-5746 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/biocell-34.pdf |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.coverage.none.fl_str_mv |
Nacional |
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Biocell |
publisher.none.fl_str_mv |
Biocell |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |