Alpha 7 nicotinic acetylcholine receptor modulates lymphocyte activation
- Autores
- de Rosa, Maria Jose; Dionisio, Leonardo Raul; Agriello, Evangelina; Bouzat, Cecilia Beatriz; Esandi, María del Carmen
- Año de publicación
- 2009
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Aims: Even though the presence of α7 nicotinic receptor (nAChR) in lymphocytes has been demonstrated, its functional role still remains elusive. The aim of our study was to characterize α7 nAChRs in human lymphocytes upon phytohemagglutinin (PHA) stimulation. Main methods: Lymphocytes were activated with the mitogen PHA. α7 nAChRs were studied by reverse transcription-polymerase chain reaction (RT-PCR), real time PCR, flow cytometry and confocal laser scanning microscopy. The effects of nicotinic drugs on PHA-induced proliferation was evaluated by the [3H]-thymidine incorporation assay. Key findings: We show that the expression of functional α7 receptors increases after PHA stimulation. The activation of peripheral lymphocytes by PHA increases 2.2-fold the α7 subunit mRNA expression and 4-fold the binding of the antagonist α-bungarotoxin (α-BTX) with respect to non activated lymphocytes. By measuring the increase of intracellular calcium in response to nicotine we determine that α7 receptors in lymphocytes are functional. Nicotinic drugs differentially modulate T cell activation. While nicotine tends to inhibit proliferative responses, specific α7 antagonists, such as α-BTX and methyllycaconitine, enhance cell division. Significance: This study reveals that the α7 receptor modulates lymphocyte activation and contributes to clarifying the role of the non neuronal cholinergic system in the immune response.
Fil: de Rosa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Dionisio, Leonardo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Agriello, Evangelina. Hospital Municipal General de Agudos Doctor José Penna; Argentina
Fil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Esandi, María del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina - Materia
-
Acetylcholine
Activation
Lymphocytes
Nicotinic Α7 Receptor - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/76597
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Alpha 7 nicotinic acetylcholine receptor modulates lymphocyte activationde Rosa, Maria JoseDionisio, Leonardo RaulAgriello, EvangelinaBouzat, Cecilia BeatrizEsandi, María del CarmenAcetylcholineActivationLymphocytesNicotinic Α7 Receptorhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Aims: Even though the presence of α7 nicotinic receptor (nAChR) in lymphocytes has been demonstrated, its functional role still remains elusive. The aim of our study was to characterize α7 nAChRs in human lymphocytes upon phytohemagglutinin (PHA) stimulation. Main methods: Lymphocytes were activated with the mitogen PHA. α7 nAChRs were studied by reverse transcription-polymerase chain reaction (RT-PCR), real time PCR, flow cytometry and confocal laser scanning microscopy. The effects of nicotinic drugs on PHA-induced proliferation was evaluated by the [3H]-thymidine incorporation assay. Key findings: We show that the expression of functional α7 receptors increases after PHA stimulation. The activation of peripheral lymphocytes by PHA increases 2.2-fold the α7 subunit mRNA expression and 4-fold the binding of the antagonist α-bungarotoxin (α-BTX) with respect to non activated lymphocytes. By measuring the increase of intracellular calcium in response to nicotine we determine that α7 receptors in lymphocytes are functional. Nicotinic drugs differentially modulate T cell activation. While nicotine tends to inhibit proliferative responses, specific α7 antagonists, such as α-BTX and methyllycaconitine, enhance cell division. Significance: This study reveals that the α7 receptor modulates lymphocyte activation and contributes to clarifying the role of the non neuronal cholinergic system in the immune response.Fil: de Rosa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Dionisio, Leonardo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Agriello, Evangelina. Hospital Municipal General de Agudos Doctor José Penna; ArgentinaFil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Esandi, María del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaPergamon-Elsevier Science Ltd2009-09-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/76597de Rosa, Maria Jose; Dionisio, Leonardo Raul; Agriello, Evangelina; Bouzat, Cecilia Beatriz; Esandi, María del Carmen; Alpha 7 nicotinic acetylcholine receptor modulates lymphocyte activation; Pergamon-Elsevier Science Ltd; Life Sciences; 85; 11-12; 9-9-2009; 444-4490024-3205CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0024320509003129info:eu-repo/semantics/altIdentifier/doi/10.1016/j.lfs.2009.07.010info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:02:11Zoai:ri.conicet.gov.ar:11336/76597instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:02:11.422CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Alpha 7 nicotinic acetylcholine receptor modulates lymphocyte activation |
title |
Alpha 7 nicotinic acetylcholine receptor modulates lymphocyte activation |
spellingShingle |
Alpha 7 nicotinic acetylcholine receptor modulates lymphocyte activation de Rosa, Maria Jose Acetylcholine Activation Lymphocytes Nicotinic Α7 Receptor |
title_short |
Alpha 7 nicotinic acetylcholine receptor modulates lymphocyte activation |
title_full |
Alpha 7 nicotinic acetylcholine receptor modulates lymphocyte activation |
title_fullStr |
Alpha 7 nicotinic acetylcholine receptor modulates lymphocyte activation |
title_full_unstemmed |
Alpha 7 nicotinic acetylcholine receptor modulates lymphocyte activation |
title_sort |
Alpha 7 nicotinic acetylcholine receptor modulates lymphocyte activation |
dc.creator.none.fl_str_mv |
de Rosa, Maria Jose Dionisio, Leonardo Raul Agriello, Evangelina Bouzat, Cecilia Beatriz Esandi, María del Carmen |
author |
de Rosa, Maria Jose |
author_facet |
de Rosa, Maria Jose Dionisio, Leonardo Raul Agriello, Evangelina Bouzat, Cecilia Beatriz Esandi, María del Carmen |
author_role |
author |
author2 |
Dionisio, Leonardo Raul Agriello, Evangelina Bouzat, Cecilia Beatriz Esandi, María del Carmen |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Acetylcholine Activation Lymphocytes Nicotinic Α7 Receptor |
topic |
Acetylcholine Activation Lymphocytes Nicotinic Α7 Receptor |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Aims: Even though the presence of α7 nicotinic receptor (nAChR) in lymphocytes has been demonstrated, its functional role still remains elusive. The aim of our study was to characterize α7 nAChRs in human lymphocytes upon phytohemagglutinin (PHA) stimulation. Main methods: Lymphocytes were activated with the mitogen PHA. α7 nAChRs were studied by reverse transcription-polymerase chain reaction (RT-PCR), real time PCR, flow cytometry and confocal laser scanning microscopy. The effects of nicotinic drugs on PHA-induced proliferation was evaluated by the [3H]-thymidine incorporation assay. Key findings: We show that the expression of functional α7 receptors increases after PHA stimulation. The activation of peripheral lymphocytes by PHA increases 2.2-fold the α7 subunit mRNA expression and 4-fold the binding of the antagonist α-bungarotoxin (α-BTX) with respect to non activated lymphocytes. By measuring the increase of intracellular calcium in response to nicotine we determine that α7 receptors in lymphocytes are functional. Nicotinic drugs differentially modulate T cell activation. While nicotine tends to inhibit proliferative responses, specific α7 antagonists, such as α-BTX and methyllycaconitine, enhance cell division. Significance: This study reveals that the α7 receptor modulates lymphocyte activation and contributes to clarifying the role of the non neuronal cholinergic system in the immune response. Fil: de Rosa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina Fil: Dionisio, Leonardo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina Fil: Agriello, Evangelina. Hospital Municipal General de Agudos Doctor José Penna; Argentina Fil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina Fil: Esandi, María del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina |
description |
Aims: Even though the presence of α7 nicotinic receptor (nAChR) in lymphocytes has been demonstrated, its functional role still remains elusive. The aim of our study was to characterize α7 nAChRs in human lymphocytes upon phytohemagglutinin (PHA) stimulation. Main methods: Lymphocytes were activated with the mitogen PHA. α7 nAChRs were studied by reverse transcription-polymerase chain reaction (RT-PCR), real time PCR, flow cytometry and confocal laser scanning microscopy. The effects of nicotinic drugs on PHA-induced proliferation was evaluated by the [3H]-thymidine incorporation assay. Key findings: We show that the expression of functional α7 receptors increases after PHA stimulation. The activation of peripheral lymphocytes by PHA increases 2.2-fold the α7 subunit mRNA expression and 4-fold the binding of the antagonist α-bungarotoxin (α-BTX) with respect to non activated lymphocytes. By measuring the increase of intracellular calcium in response to nicotine we determine that α7 receptors in lymphocytes are functional. Nicotinic drugs differentially modulate T cell activation. While nicotine tends to inhibit proliferative responses, specific α7 antagonists, such as α-BTX and methyllycaconitine, enhance cell division. Significance: This study reveals that the α7 receptor modulates lymphocyte activation and contributes to clarifying the role of the non neuronal cholinergic system in the immune response. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-09-09 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/76597 de Rosa, Maria Jose; Dionisio, Leonardo Raul; Agriello, Evangelina; Bouzat, Cecilia Beatriz; Esandi, María del Carmen; Alpha 7 nicotinic acetylcholine receptor modulates lymphocyte activation; Pergamon-Elsevier Science Ltd; Life Sciences; 85; 11-12; 9-9-2009; 444-449 0024-3205 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/76597 |
identifier_str_mv |
de Rosa, Maria Jose; Dionisio, Leonardo Raul; Agriello, Evangelina; Bouzat, Cecilia Beatriz; Esandi, María del Carmen; Alpha 7 nicotinic acetylcholine receptor modulates lymphocyte activation; Pergamon-Elsevier Science Ltd; Life Sciences; 85; 11-12; 9-9-2009; 444-449 0024-3205 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0024320509003129 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.lfs.2009.07.010 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Pergamon-Elsevier Science Ltd |
publisher.none.fl_str_mv |
Pergamon-Elsevier Science Ltd |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842269742264483840 |
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13.13397 |