Neuronal cytoskeletal alterations in an experimental model of depression

Autores
Reines, Analia Gabriela; Cereseto, Marina; Ferrero, Alejandro Javier; Bonavita, C.; Wikinski, Silvia Ines
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
It has been proposed that depression is associated with hippocampal morphological changes. The apical dendrite atrophy of hippocampal CA3 pyramidal neurons has been described in experimental models of depression. The aim of the present study was to determine which cytoskeletal components are involved in the morphological changes previously described in the hippocampus of depressed animals. The expression of different neuronal cytoskeletal markers was analyzed by immunohistochemistry in rats exposed to a learned helplessness paradigm, an experimental model of depression. Rats were trained with 60 inescapable foot shocks (0.6 mA/15 s) and escape latencies and failures were tested 4 days after training. Animals in which learned helplessness behavior persisted for 21 days were included in the depressed group. No foot shocks were delivered to control rats. Microtubule-associated protein 2 (MAP-2) and light (NFL; 68 kDa), medium (NFM; 160 kDa) and heavy (NFH; 200 kDa) neurofilament subunit immunostainings were analyzed employing morphometric parameters. In the depressed group, NFL immunostaining decreased 55% (P<0.05) and 60% (P<0.001) in CA3 and dentate gyrus, respectively. In the same areas, MAP-2, NFM and NFH immunostainings did not differ between depressed and control animals. Since NFL is present in the core of mature neurofilament, it is proposed that hippocampal depression-associated plastic alterations may be due to changes in the dynamics of the neurofilament assembly.
Fil: Reines, Analia Gabriela.
Fil: Marina Cereseto. ININFA; Argentina
Fil: Ferrero, Alejandro Javier. ININFA; Argentina
Fil: Carla Bonavita. ININFA; Argentina
Fil: Wikinski, Silvia Ines. ININFA; Argentina
Materia
HIPPOCAMPAL ATROPHY
HIPPOCAMPAL PLASTICITY
INTERMEDIATE FILAMENTS
LEARNED HELPLESSNESS PARADIGM
LIGHT NEUROFILAMENT SUBUNIT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/94569
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/94569
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Neuronal cytoskeletal alterations in an experimental model of depressionReines, Analia GabrielaCereseto, MarinaFerrero, Alejandro JavierBonavita, C.Wikinski, Silvia InesHIPPOCAMPAL ATROPHYHIPPOCAMPAL PLASTICITYINTERMEDIATE FILAMENTSLEARNED HELPLESSNESS PARADIGMLIGHT NEUROFILAMENT SUBUNIThttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3It has been proposed that depression is associated with hippocampal morphological changes. The apical dendrite atrophy of hippocampal CA3 pyramidal neurons has been described in experimental models of depression. The aim of the present study was to determine which cytoskeletal components are involved in the morphological changes previously described in the hippocampus of depressed animals. The expression of different neuronal cytoskeletal markers was analyzed by immunohistochemistry in rats exposed to a learned helplessness paradigm, an experimental model of depression. Rats were trained with 60 inescapable foot shocks (0.6 mA/15 s) and escape latencies and failures were tested 4 days after training. Animals in which learned helplessness behavior persisted for 21 days were included in the depressed group. No foot shocks were delivered to control rats. Microtubule-associated protein 2 (MAP-2) and light (NFL; 68 kDa), medium (NFM; 160 kDa) and heavy (NFH; 200 kDa) neurofilament subunit immunostainings were analyzed employing morphometric parameters. In the depressed group, NFL immunostaining decreased 55% (P<0.05) and 60% (P<0.001) in CA3 and dentate gyrus, respectively. In the same areas, MAP-2, NFM and NFH immunostainings did not differ between depressed and control animals. Since NFL is present in the core of mature neurofilament, it is proposed that hippocampal depression-associated plastic alterations may be due to changes in the dynamics of the neurofilament assembly.Fil: Reines, Analia Gabriela.Fil: Marina Cereseto. ININFA; ArgentinaFil: Ferrero, Alejandro Javier. ININFA; ArgentinaFil: Carla Bonavita. ININFA; ArgentinaFil: Wikinski, Silvia Ines. ININFA; ArgentinaPergamon-Elsevier Science Ltd2004-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/94569Reines, Analia Gabriela; Cereseto, Marina; Ferrero, Alejandro Javier; Bonavita, C.; Wikinski, Silvia Ines; Neuronal cytoskeletal alterations in an experimental model of depression; Pergamon-Elsevier Science Ltd; Neuroscience; 129; 3; 12-2004; 529-5380306-4522CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0306452204007365info:eu-repo/semantics/altIdentifier/doi/10.1016/j.neuroscience.2004.08.026info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:50:40Zoai:ri.conicet.gov.ar:11336/94569instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:50:40.339CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Neuronal cytoskeletal alterations in an experimental model of depression
title Neuronal cytoskeletal alterations in an experimental model of depression
spellingShingle Neuronal cytoskeletal alterations in an experimental model of depression
Reines, Analia Gabriela
HIPPOCAMPAL ATROPHY
HIPPOCAMPAL PLASTICITY
INTERMEDIATE FILAMENTS
LEARNED HELPLESSNESS PARADIGM
LIGHT NEUROFILAMENT SUBUNIT
title_short Neuronal cytoskeletal alterations in an experimental model of depression
title_full Neuronal cytoskeletal alterations in an experimental model of depression
title_fullStr Neuronal cytoskeletal alterations in an experimental model of depression
title_full_unstemmed Neuronal cytoskeletal alterations in an experimental model of depression
title_sort Neuronal cytoskeletal alterations in an experimental model of depression
dc.creator.none.fl_str_mv Reines, Analia Gabriela
Cereseto, Marina
Ferrero, Alejandro Javier
Bonavita, C.
Wikinski, Silvia Ines
author Reines, Analia Gabriela
author_facet Reines, Analia Gabriela
Cereseto, Marina
Ferrero, Alejandro Javier
Bonavita, C.
Wikinski, Silvia Ines
author_role author
author2 Cereseto, Marina
Ferrero, Alejandro Javier
Bonavita, C.
Wikinski, Silvia Ines
author2_role author
author
author
author
dc.subject.none.fl_str_mv HIPPOCAMPAL ATROPHY
HIPPOCAMPAL PLASTICITY
INTERMEDIATE FILAMENTS
LEARNED HELPLESSNESS PARADIGM
LIGHT NEUROFILAMENT SUBUNIT
topic HIPPOCAMPAL ATROPHY
HIPPOCAMPAL PLASTICITY
INTERMEDIATE FILAMENTS
LEARNED HELPLESSNESS PARADIGM
LIGHT NEUROFILAMENT SUBUNIT
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv It has been proposed that depression is associated with hippocampal morphological changes. The apical dendrite atrophy of hippocampal CA3 pyramidal neurons has been described in experimental models of depression. The aim of the present study was to determine which cytoskeletal components are involved in the morphological changes previously described in the hippocampus of depressed animals. The expression of different neuronal cytoskeletal markers was analyzed by immunohistochemistry in rats exposed to a learned helplessness paradigm, an experimental model of depression. Rats were trained with 60 inescapable foot shocks (0.6 mA/15 s) and escape latencies and failures were tested 4 days after training. Animals in which learned helplessness behavior persisted for 21 days were included in the depressed group. No foot shocks were delivered to control rats. Microtubule-associated protein 2 (MAP-2) and light (NFL; 68 kDa), medium (NFM; 160 kDa) and heavy (NFH; 200 kDa) neurofilament subunit immunostainings were analyzed employing morphometric parameters. In the depressed group, NFL immunostaining decreased 55% (P<0.05) and 60% (P<0.001) in CA3 and dentate gyrus, respectively. In the same areas, MAP-2, NFM and NFH immunostainings did not differ between depressed and control animals. Since NFL is present in the core of mature neurofilament, it is proposed that hippocampal depression-associated plastic alterations may be due to changes in the dynamics of the neurofilament assembly.
Fil: Reines, Analia Gabriela.
Fil: Marina Cereseto. ININFA; Argentina
Fil: Ferrero, Alejandro Javier. ININFA; Argentina
Fil: Carla Bonavita. ININFA; Argentina
Fil: Wikinski, Silvia Ines. ININFA; Argentina
description It has been proposed that depression is associated with hippocampal morphological changes. The apical dendrite atrophy of hippocampal CA3 pyramidal neurons has been described in experimental models of depression. The aim of the present study was to determine which cytoskeletal components are involved in the morphological changes previously described in the hippocampus of depressed animals. The expression of different neuronal cytoskeletal markers was analyzed by immunohistochemistry in rats exposed to a learned helplessness paradigm, an experimental model of depression. Rats were trained with 60 inescapable foot shocks (0.6 mA/15 s) and escape latencies and failures were tested 4 days after training. Animals in which learned helplessness behavior persisted for 21 days were included in the depressed group. No foot shocks were delivered to control rats. Microtubule-associated protein 2 (MAP-2) and light (NFL; 68 kDa), medium (NFM; 160 kDa) and heavy (NFH; 200 kDa) neurofilament subunit immunostainings were analyzed employing morphometric parameters. In the depressed group, NFL immunostaining decreased 55% (P<0.05) and 60% (P<0.001) in CA3 and dentate gyrus, respectively. In the same areas, MAP-2, NFM and NFH immunostainings did not differ between depressed and control animals. Since NFL is present in the core of mature neurofilament, it is proposed that hippocampal depression-associated plastic alterations may be due to changes in the dynamics of the neurofilament assembly.
publishDate 2004
dc.date.none.fl_str_mv 2004-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/94569
Reines, Analia Gabriela; Cereseto, Marina; Ferrero, Alejandro Javier; Bonavita, C.; Wikinski, Silvia Ines; Neuronal cytoskeletal alterations in an experimental model of depression; Pergamon-Elsevier Science Ltd; Neuroscience; 129; 3; 12-2004; 529-538
0306-4522
CONICET Digital
CONICET
url http://hdl.handle.net/11336/94569
identifier_str_mv Reines, Analia Gabriela; Cereseto, Marina; Ferrero, Alejandro Javier; Bonavita, C.; Wikinski, Silvia Ines; Neuronal cytoskeletal alterations in an experimental model of depression; Pergamon-Elsevier Science Ltd; Neuroscience; 129; 3; 12-2004; 529-538
0306-4522
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0306452204007365
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.neuroscience.2004.08.026
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269045757313024
score 13.13397

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