Cholesterol exerts different modulatory effects at the cell surface of muscle and neuronal nicotinic acetylcholine receptors
- Autores
- Borroni, Maria Virginia; Barrantes, Francisco Jose
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Communication at synapses requires the location and maintenanceof receptors at specific sites. Factors controlling the distribution ofreceptors are critical determinants of the cell response to externalsignals. The nicotinic acetylcholine receptor (AChR) is the bestcharacterized ligand-gated ion channel, found at neuromuscularjunctions and central nervous system synapses. We have reportedthat cholesterol contributes to the homeostasis of muscle-typereceptor levels at the plasmalemma (Kumari et al., J. Cell Biol.,2008; Borroni and Barrantes, J. Biol. Chem., 2011). Cyclodextrin-mediated acute cholesterol depletion lowered the number of cell-surface muscle AChRs in CHO-K1/A5 cells and C2C12 musclecells. In contrast, the same treatment, performed on culturedhippocampal neurons, increased the amount ofa7 AChR at theplasmalemma. In addition, chronic cholesterol reduction mediatedby Mevinolin, an inhibitor of cholesterol biosynthesis, augmentedthe cell-surface levels ofa7 AChR anda4b2 neuronal AChRsubtypes measured by fluorescence microscopy and radioactiveligand binding assays. The results suggest that cholesterol modu-lates surface AChR levels differentially in peripheral and centralcholinergic synapses
Fil: Borroni, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Barrantes, Francisco Jose. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
5th Special Conference of the International Society for Neurochemistry, and dendritic spines in health and disease
Buenos Aires
Argentina
International Society for Neurochemistry - Materia
-
NICOTINIC ACETYLCHOLINE RECEPTOR
CHOLESTEROL
HIPPOCAMPAL NEURONS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/232877
Ver los metadatos del registro completo
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Cholesterol exerts different modulatory effects at the cell surface of muscle and neuronal nicotinic acetylcholine receptorsBorroni, Maria VirginiaBarrantes, Francisco JoseNICOTINIC ACETYLCHOLINE RECEPTORCHOLESTEROLHIPPOCAMPAL NEURONShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Communication at synapses requires the location and maintenanceof receptors at specific sites. Factors controlling the distribution ofreceptors are critical determinants of the cell response to externalsignals. The nicotinic acetylcholine receptor (AChR) is the bestcharacterized ligand-gated ion channel, found at neuromuscularjunctions and central nervous system synapses. We have reportedthat cholesterol contributes to the homeostasis of muscle-typereceptor levels at the plasmalemma (Kumari et al., J. Cell Biol.,2008; Borroni and Barrantes, J. Biol. Chem., 2011). Cyclodextrin-mediated acute cholesterol depletion lowered the number of cell-surface muscle AChRs in CHO-K1/A5 cells and C2C12 musclecells. In contrast, the same treatment, performed on culturedhippocampal neurons, increased the amount ofa7 AChR at theplasmalemma. In addition, chronic cholesterol reduction mediatedby Mevinolin, an inhibitor of cholesterol biosynthesis, augmentedthe cell-surface levels ofa7 AChR anda4b2 neuronal AChRsubtypes measured by fluorescence microscopy and radioactiveligand binding assays. The results suggest that cholesterol modu-lates surface AChR levels differentially in peripheral and centralcholinergic synapsesFil: Borroni, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Barrantes, Francisco Jose. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina5th Special Conference of the International Society for Neurochemistry, and dendritic spines in health and diseaseBuenos AiresArgentinaInternational Society for NeurochemistryWiley Blackwell Publishing, Inc2012info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/232877Cholesterol exerts different modulatory effects at the cell surface of muscle and neuronal nicotinic acetylcholine receptors; 5th Special Conference of the International Society for Neurochemistry, and dendritic spines in health and disease; Buenos Aires; Argentina; 2012; 13-131471-4159CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/j.1471-4159.2012.07848_1.xinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1471-4159.2012.07848_1.xInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:53Zoai:ri.conicet.gov.ar:11336/232877instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:54.213CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Cholesterol exerts different modulatory effects at the cell surface of muscle and neuronal nicotinic acetylcholine receptors |
title |
Cholesterol exerts different modulatory effects at the cell surface of muscle and neuronal nicotinic acetylcholine receptors |
spellingShingle |
Cholesterol exerts different modulatory effects at the cell surface of muscle and neuronal nicotinic acetylcholine receptors Borroni, Maria Virginia NICOTINIC ACETYLCHOLINE RECEPTOR CHOLESTEROL HIPPOCAMPAL NEURONS |
title_short |
Cholesterol exerts different modulatory effects at the cell surface of muscle and neuronal nicotinic acetylcholine receptors |
title_full |
Cholesterol exerts different modulatory effects at the cell surface of muscle and neuronal nicotinic acetylcholine receptors |
title_fullStr |
Cholesterol exerts different modulatory effects at the cell surface of muscle and neuronal nicotinic acetylcholine receptors |
title_full_unstemmed |
Cholesterol exerts different modulatory effects at the cell surface of muscle and neuronal nicotinic acetylcholine receptors |
title_sort |
Cholesterol exerts different modulatory effects at the cell surface of muscle and neuronal nicotinic acetylcholine receptors |
dc.creator.none.fl_str_mv |
Borroni, Maria Virginia Barrantes, Francisco Jose |
author |
Borroni, Maria Virginia |
author_facet |
Borroni, Maria Virginia Barrantes, Francisco Jose |
author_role |
author |
author2 |
Barrantes, Francisco Jose |
author2_role |
author |
dc.subject.none.fl_str_mv |
NICOTINIC ACETYLCHOLINE RECEPTOR CHOLESTEROL HIPPOCAMPAL NEURONS |
topic |
NICOTINIC ACETYLCHOLINE RECEPTOR CHOLESTEROL HIPPOCAMPAL NEURONS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Communication at synapses requires the location and maintenanceof receptors at specific sites. Factors controlling the distribution ofreceptors are critical determinants of the cell response to externalsignals. The nicotinic acetylcholine receptor (AChR) is the bestcharacterized ligand-gated ion channel, found at neuromuscularjunctions and central nervous system synapses. We have reportedthat cholesterol contributes to the homeostasis of muscle-typereceptor levels at the plasmalemma (Kumari et al., J. Cell Biol.,2008; Borroni and Barrantes, J. Biol. Chem., 2011). Cyclodextrin-mediated acute cholesterol depletion lowered the number of cell-surface muscle AChRs in CHO-K1/A5 cells and C2C12 musclecells. In contrast, the same treatment, performed on culturedhippocampal neurons, increased the amount ofa7 AChR at theplasmalemma. In addition, chronic cholesterol reduction mediatedby Mevinolin, an inhibitor of cholesterol biosynthesis, augmentedthe cell-surface levels ofa7 AChR anda4b2 neuronal AChRsubtypes measured by fluorescence microscopy and radioactiveligand binding assays. The results suggest that cholesterol modu-lates surface AChR levels differentially in peripheral and centralcholinergic synapses Fil: Borroni, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina Fil: Barrantes, Francisco Jose. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 5th Special Conference of the International Society for Neurochemistry, and dendritic spines in health and disease Buenos Aires Argentina International Society for Neurochemistry |
description |
Communication at synapses requires the location and maintenanceof receptors at specific sites. Factors controlling the distribution ofreceptors are critical determinants of the cell response to externalsignals. The nicotinic acetylcholine receptor (AChR) is the bestcharacterized ligand-gated ion channel, found at neuromuscularjunctions and central nervous system synapses. We have reportedthat cholesterol contributes to the homeostasis of muscle-typereceptor levels at the plasmalemma (Kumari et al., J. Cell Biol.,2008; Borroni and Barrantes, J. Biol. Chem., 2011). Cyclodextrin-mediated acute cholesterol depletion lowered the number of cell-surface muscle AChRs in CHO-K1/A5 cells and C2C12 musclecells. In contrast, the same treatment, performed on culturedhippocampal neurons, increased the amount ofa7 AChR at theplasmalemma. In addition, chronic cholesterol reduction mediatedby Mevinolin, an inhibitor of cholesterol biosynthesis, augmentedthe cell-surface levels ofa7 AChR anda4b2 neuronal AChRsubtypes measured by fluorescence microscopy and radioactiveligand binding assays. The results suggest that cholesterol modu-lates surface AChR levels differentially in peripheral and centralcholinergic synapses |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Congreso Journal http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
status_str |
publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/232877 Cholesterol exerts different modulatory effects at the cell surface of muscle and neuronal nicotinic acetylcholine receptors; 5th Special Conference of the International Society for Neurochemistry, and dendritic spines in health and disease; Buenos Aires; Argentina; 2012; 13-13 1471-4159 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/232877 |
identifier_str_mv |
Cholesterol exerts different modulatory effects at the cell surface of muscle and neuronal nicotinic acetylcholine receptors; 5th Special Conference of the International Society for Neurochemistry, and dendritic spines in health and disease; Buenos Aires; Argentina; 2012; 13-13 1471-4159 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/j.1471-4159.2012.07848_1.x info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1471-4159.2012.07848_1.x |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.coverage.none.fl_str_mv |
Internacional |
dc.publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.13397 |