Cholesterol exerts different modulatory effects at the cell surface of muscle and neuronal nicotinic acetylcholine receptors

Autores
Borroni, Maria Virginia; Barrantes, Francisco Jose
Año de publicación
2012
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Communication at synapses requires the location and maintenanceof receptors at specific sites. Factors controlling the distribution ofreceptors are critical determinants of the cell response to externalsignals. The nicotinic acetylcholine receptor (AChR) is the bestcharacterized ligand-gated ion channel, found at neuromuscularjunctions and central nervous system synapses. We have reportedthat cholesterol contributes to the homeostasis of muscle-typereceptor levels at the plasmalemma (Kumari et al., J. Cell Biol.,2008; Borroni and Barrantes, J. Biol. Chem., 2011). Cyclodextrin-mediated acute cholesterol depletion lowered the number of cell-surface muscle AChRs in CHO-K1/A5 cells and C2C12 musclecells. In contrast, the same treatment, performed on culturedhippocampal neurons, increased the amount ofa7 AChR at theplasmalemma. In addition, chronic cholesterol reduction mediatedby Mevinolin, an inhibitor of cholesterol biosynthesis, augmentedthe cell-surface levels ofa7 AChR anda4b2 neuronal AChRsubtypes measured by fluorescence microscopy and radioactiveligand binding assays. The results suggest that cholesterol modu-lates surface AChR levels differentially in peripheral and centralcholinergic synapses
Fil: Borroni, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Barrantes, Francisco Jose. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
5th Special Conference of the International Society for Neurochemistry, and dendritic spines in health and disease
Buenos Aires
Argentina
International Society for Neurochemistry
Materia
NICOTINIC ACETYLCHOLINE RECEPTOR
CHOLESTEROL
HIPPOCAMPAL NEURONS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/232877
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Cholesterol exerts different modulatory effects at the cell surface of muscle and neuronal nicotinic acetylcholine receptorsBorroni, Maria VirginiaBarrantes, Francisco JoseNICOTINIC ACETYLCHOLINE RECEPTORCHOLESTEROLHIPPOCAMPAL NEURONShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Communication at synapses requires the location and maintenanceof receptors at specific sites. Factors controlling the distribution ofreceptors are critical determinants of the cell response to externalsignals. The nicotinic acetylcholine receptor (AChR) is the bestcharacterized ligand-gated ion channel, found at neuromuscularjunctions and central nervous system synapses. We have reportedthat cholesterol contributes to the homeostasis of muscle-typereceptor levels at the plasmalemma (Kumari et al., J. Cell Biol.,2008; Borroni and Barrantes, J. Biol. Chem., 2011). Cyclodextrin-mediated acute cholesterol depletion lowered the number of cell-surface muscle AChRs in CHO-K1/A5 cells and C2C12 musclecells. In contrast, the same treatment, performed on culturedhippocampal neurons, increased the amount ofa7 AChR at theplasmalemma. In addition, chronic cholesterol reduction mediatedby Mevinolin, an inhibitor of cholesterol biosynthesis, augmentedthe cell-surface levels ofa7 AChR anda4b2 neuronal AChRsubtypes measured by fluorescence microscopy and radioactiveligand binding assays. The results suggest that cholesterol modu-lates surface AChR levels differentially in peripheral and centralcholinergic synapsesFil: Borroni, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Barrantes, Francisco Jose. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina5th Special Conference of the International Society for Neurochemistry, and dendritic spines in health and diseaseBuenos AiresArgentinaInternational Society for NeurochemistryWiley Blackwell Publishing, Inc2012info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/232877Cholesterol exerts different modulatory effects at the cell surface of muscle and neuronal nicotinic acetylcholine receptors; 5th Special Conference of the International Society for Neurochemistry, and dendritic spines in health and disease; Buenos Aires; Argentina; 2012; 13-131471-4159CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/j.1471-4159.2012.07848_1.xinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1471-4159.2012.07848_1.xInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:40:27Zoai:ri.conicet.gov.ar:11336/232877instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:40:27.709CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cholesterol exerts different modulatory effects at the cell surface of muscle and neuronal nicotinic acetylcholine receptors
title Cholesterol exerts different modulatory effects at the cell surface of muscle and neuronal nicotinic acetylcholine receptors
spellingShingle Cholesterol exerts different modulatory effects at the cell surface of muscle and neuronal nicotinic acetylcholine receptors
Borroni, Maria Virginia
NICOTINIC ACETYLCHOLINE RECEPTOR
CHOLESTEROL
HIPPOCAMPAL NEURONS
title_short Cholesterol exerts different modulatory effects at the cell surface of muscle and neuronal nicotinic acetylcholine receptors
title_full Cholesterol exerts different modulatory effects at the cell surface of muscle and neuronal nicotinic acetylcholine receptors
title_fullStr Cholesterol exerts different modulatory effects at the cell surface of muscle and neuronal nicotinic acetylcholine receptors
title_full_unstemmed Cholesterol exerts different modulatory effects at the cell surface of muscle and neuronal nicotinic acetylcholine receptors
title_sort Cholesterol exerts different modulatory effects at the cell surface of muscle and neuronal nicotinic acetylcholine receptors
dc.creator.none.fl_str_mv Borroni, Maria Virginia
Barrantes, Francisco Jose
author Borroni, Maria Virginia
author_facet Borroni, Maria Virginia
Barrantes, Francisco Jose
author_role author
author2 Barrantes, Francisco Jose
author2_role author
dc.subject.none.fl_str_mv NICOTINIC ACETYLCHOLINE RECEPTOR
CHOLESTEROL
HIPPOCAMPAL NEURONS
topic NICOTINIC ACETYLCHOLINE RECEPTOR
CHOLESTEROL
HIPPOCAMPAL NEURONS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Communication at synapses requires the location and maintenanceof receptors at specific sites. Factors controlling the distribution ofreceptors are critical determinants of the cell response to externalsignals. The nicotinic acetylcholine receptor (AChR) is the bestcharacterized ligand-gated ion channel, found at neuromuscularjunctions and central nervous system synapses. We have reportedthat cholesterol contributes to the homeostasis of muscle-typereceptor levels at the plasmalemma (Kumari et al., J. Cell Biol.,2008; Borroni and Barrantes, J. Biol. Chem., 2011). Cyclodextrin-mediated acute cholesterol depletion lowered the number of cell-surface muscle AChRs in CHO-K1/A5 cells and C2C12 musclecells. In contrast, the same treatment, performed on culturedhippocampal neurons, increased the amount ofa7 AChR at theplasmalemma. In addition, chronic cholesterol reduction mediatedby Mevinolin, an inhibitor of cholesterol biosynthesis, augmentedthe cell-surface levels ofa7 AChR anda4b2 neuronal AChRsubtypes measured by fluorescence microscopy and radioactiveligand binding assays. The results suggest that cholesterol modu-lates surface AChR levels differentially in peripheral and centralcholinergic synapses
Fil: Borroni, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Barrantes, Francisco Jose. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
5th Special Conference of the International Society for Neurochemistry, and dendritic spines in health and disease
Buenos Aires
Argentina
International Society for Neurochemistry
description Communication at synapses requires the location and maintenanceof receptors at specific sites. Factors controlling the distribution ofreceptors are critical determinants of the cell response to externalsignals. The nicotinic acetylcholine receptor (AChR) is the bestcharacterized ligand-gated ion channel, found at neuromuscularjunctions and central nervous system synapses. We have reportedthat cholesterol contributes to the homeostasis of muscle-typereceptor levels at the plasmalemma (Kumari et al., J. Cell Biol.,2008; Borroni and Barrantes, J. Biol. Chem., 2011). Cyclodextrin-mediated acute cholesterol depletion lowered the number of cell-surface muscle AChRs in CHO-K1/A5 cells and C2C12 musclecells. In contrast, the same treatment, performed on culturedhippocampal neurons, increased the amount ofa7 AChR at theplasmalemma. In addition, chronic cholesterol reduction mediatedby Mevinolin, an inhibitor of cholesterol biosynthesis, augmentedthe cell-surface levels ofa7 AChR anda4b2 neuronal AChRsubtypes measured by fluorescence microscopy and radioactiveligand binding assays. The results suggest that cholesterol modu-lates surface AChR levels differentially in peripheral and centralcholinergic synapses
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/232877
Cholesterol exerts different modulatory effects at the cell surface of muscle and neuronal nicotinic acetylcholine receptors; 5th Special Conference of the International Society for Neurochemistry, and dendritic spines in health and disease; Buenos Aires; Argentina; 2012; 13-13
1471-4159
CONICET Digital
CONICET
url http://hdl.handle.net/11336/232877
identifier_str_mv Cholesterol exerts different modulatory effects at the cell surface of muscle and neuronal nicotinic acetylcholine receptors; 5th Special Conference of the International Society for Neurochemistry, and dendritic spines in health and disease; Buenos Aires; Argentina; 2012; 13-13
1471-4159
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/j.1471-4159.2012.07848_1.x
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1471-4159.2012.07848_1.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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