Acetylcholinesterase inhibition interacts with training to reverse spatial learning deficits after cortical impact injury
- Autores
- Scremin, Oscar Umberto; Norman, Keith M.; Roch, Margareth; Holschneider, Daniel P.; Scremin, A. M. Erika
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Cholinergic mechanisms are known to play a key role in cognitive functions that are profoundly altered in traumatic brain injury (TBI). The present investigation was designed to test the ability of continuous administration, starting at the time of injury, of physostigmine (PHY), an acetylcholinesterase (AChE) inhibitor that crosses the blood-brain barrier (BBB), to ameliorate the alterations of learning and memory induced by cerebral cortex impact injury in rats under isoflurane anesthesia. Learning and memory were assessed with the Morris water maze implemented during days 7-11 (WM1), and days 21-25 post-TBI (WM2), with four trials per day for 3 days, followed by target reversal and 2 additional days of training. These groups of Sprague-Dawley male rats were used: TBI treated with PHY at 3.2 μmol/kg/day (TBI-PHY3.2), or 6.4 μmol/kg/day (TBI-PHY6.4), by subcutaneous osmotic pumps, or TBI and no injury (Sham) treated with saline. AChE activity was measured in brain tissue samples of non-traumatized animals that received PHY at the doses used in the TBI animals. In WM1 tests, PHY3.2 improved learning within sessions, but not between sessions, in the recall of the target position, while PHY6.4 had no significant effects. In WM2 tests, PHY improved within- and between-sessions performance at both dose levels. We found that continuous AChE inhibition interacted with repeated training on the water maze task to completely reverse the deficits seen in learning and memory induced by TBI. The PHY treatment also reduced the amount of brain tissue loss as measured using cresyl violet staining.
Fil: Scremin, Oscar Umberto. University of California at Los Angeles. School of Medicine; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Norman, Keith M.. No especifíca;
Fil: Roch, Margareth. No especifíca;
Fil: Holschneider, Daniel P.. No especifíca;
Fil: Scremin, A. M. Erika. University of California at Los Angeles. School of Medicine; Estados Unidos - Materia
-
ACETYLCHOLINESTERASE
CHOLINERGIC
NEUROREHABILITATION
SPATIAL LEARNING
TRAUMATIC BRAIN INJURY - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/197514
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Acetylcholinesterase inhibition interacts with training to reverse spatial learning deficits after cortical impact injuryScremin, Oscar UmbertoNorman, Keith M.Roch, MargarethHolschneider, Daniel P.Scremin, A. M. ErikaACETYLCHOLINESTERASECHOLINERGICNEUROREHABILITATIONSPATIAL LEARNINGTRAUMATIC BRAIN INJURYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Cholinergic mechanisms are known to play a key role in cognitive functions that are profoundly altered in traumatic brain injury (TBI). The present investigation was designed to test the ability of continuous administration, starting at the time of injury, of physostigmine (PHY), an acetylcholinesterase (AChE) inhibitor that crosses the blood-brain barrier (BBB), to ameliorate the alterations of learning and memory induced by cerebral cortex impact injury in rats under isoflurane anesthesia. Learning and memory were assessed with the Morris water maze implemented during days 7-11 (WM1), and days 21-25 post-TBI (WM2), with four trials per day for 3 days, followed by target reversal and 2 additional days of training. These groups of Sprague-Dawley male rats were used: TBI treated with PHY at 3.2 μmol/kg/day (TBI-PHY3.2), or 6.4 μmol/kg/day (TBI-PHY6.4), by subcutaneous osmotic pumps, or TBI and no injury (Sham) treated with saline. AChE activity was measured in brain tissue samples of non-traumatized animals that received PHY at the doses used in the TBI animals. In WM1 tests, PHY3.2 improved learning within sessions, but not between sessions, in the recall of the target position, while PHY6.4 had no significant effects. In WM2 tests, PHY improved within- and between-sessions performance at both dose levels. We found that continuous AChE inhibition interacted with repeated training on the water maze task to completely reverse the deficits seen in learning and memory induced by TBI. The PHY treatment also reduced the amount of brain tissue loss as measured using cresyl violet staining.Fil: Scremin, Oscar Umberto. University of California at Los Angeles. School of Medicine; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFil: Norman, Keith M.. No especifíca;Fil: Roch, Margareth. No especifíca;Fil: Holschneider, Daniel P.. No especifíca;Fil: Scremin, A. M. Erika. University of California at Los Angeles. School of Medicine; Estados UnidosMary Ann Liebert2012-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/197514Scremin, Oscar Umberto; Norman, Keith M.; Roch, Margareth; Holschneider, Daniel P.; Scremin, A. M. Erika; Acetylcholinesterase inhibition interacts with training to reverse spatial learning deficits after cortical impact injury; Mary Ann Liebert; Journal of Neurotrauma; 29; 15; 10-2012; 2457-24640897-7151CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.liebertpub.com/doi/10.1089/neu.2012.2465info:eu-repo/semantics/altIdentifier/doi/10.1089/neu.2012.2465info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:40:27Zoai:ri.conicet.gov.ar:11336/197514instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:40:27.944CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Acetylcholinesterase inhibition interacts with training to reverse spatial learning deficits after cortical impact injury |
title |
Acetylcholinesterase inhibition interacts with training to reverse spatial learning deficits after cortical impact injury |
spellingShingle |
Acetylcholinesterase inhibition interacts with training to reverse spatial learning deficits after cortical impact injury Scremin, Oscar Umberto ACETYLCHOLINESTERASE CHOLINERGIC NEUROREHABILITATION SPATIAL LEARNING TRAUMATIC BRAIN INJURY |
title_short |
Acetylcholinesterase inhibition interacts with training to reverse spatial learning deficits after cortical impact injury |
title_full |
Acetylcholinesterase inhibition interacts with training to reverse spatial learning deficits after cortical impact injury |
title_fullStr |
Acetylcholinesterase inhibition interacts with training to reverse spatial learning deficits after cortical impact injury |
title_full_unstemmed |
Acetylcholinesterase inhibition interacts with training to reverse spatial learning deficits after cortical impact injury |
title_sort |
Acetylcholinesterase inhibition interacts with training to reverse spatial learning deficits after cortical impact injury |
dc.creator.none.fl_str_mv |
Scremin, Oscar Umberto Norman, Keith M. Roch, Margareth Holschneider, Daniel P. Scremin, A. M. Erika |
author |
Scremin, Oscar Umberto |
author_facet |
Scremin, Oscar Umberto Norman, Keith M. Roch, Margareth Holschneider, Daniel P. Scremin, A. M. Erika |
author_role |
author |
author2 |
Norman, Keith M. Roch, Margareth Holschneider, Daniel P. Scremin, A. M. Erika |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
ACETYLCHOLINESTERASE CHOLINERGIC NEUROREHABILITATION SPATIAL LEARNING TRAUMATIC BRAIN INJURY |
topic |
ACETYLCHOLINESTERASE CHOLINERGIC NEUROREHABILITATION SPATIAL LEARNING TRAUMATIC BRAIN INJURY |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Cholinergic mechanisms are known to play a key role in cognitive functions that are profoundly altered in traumatic brain injury (TBI). The present investigation was designed to test the ability of continuous administration, starting at the time of injury, of physostigmine (PHY), an acetylcholinesterase (AChE) inhibitor that crosses the blood-brain barrier (BBB), to ameliorate the alterations of learning and memory induced by cerebral cortex impact injury in rats under isoflurane anesthesia. Learning and memory were assessed with the Morris water maze implemented during days 7-11 (WM1), and days 21-25 post-TBI (WM2), with four trials per day for 3 days, followed by target reversal and 2 additional days of training. These groups of Sprague-Dawley male rats were used: TBI treated with PHY at 3.2 μmol/kg/day (TBI-PHY3.2), or 6.4 μmol/kg/day (TBI-PHY6.4), by subcutaneous osmotic pumps, or TBI and no injury (Sham) treated with saline. AChE activity was measured in brain tissue samples of non-traumatized animals that received PHY at the doses used in the TBI animals. In WM1 tests, PHY3.2 improved learning within sessions, but not between sessions, in the recall of the target position, while PHY6.4 had no significant effects. In WM2 tests, PHY improved within- and between-sessions performance at both dose levels. We found that continuous AChE inhibition interacted with repeated training on the water maze task to completely reverse the deficits seen in learning and memory induced by TBI. The PHY treatment also reduced the amount of brain tissue loss as measured using cresyl violet staining. Fil: Scremin, Oscar Umberto. University of California at Los Angeles. School of Medicine; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina Fil: Norman, Keith M.. No especifíca; Fil: Roch, Margareth. No especifíca; Fil: Holschneider, Daniel P.. No especifíca; Fil: Scremin, A. M. Erika. University of California at Los Angeles. School of Medicine; Estados Unidos |
description |
Cholinergic mechanisms are known to play a key role in cognitive functions that are profoundly altered in traumatic brain injury (TBI). The present investigation was designed to test the ability of continuous administration, starting at the time of injury, of physostigmine (PHY), an acetylcholinesterase (AChE) inhibitor that crosses the blood-brain barrier (BBB), to ameliorate the alterations of learning and memory induced by cerebral cortex impact injury in rats under isoflurane anesthesia. Learning and memory were assessed with the Morris water maze implemented during days 7-11 (WM1), and days 21-25 post-TBI (WM2), with four trials per day for 3 days, followed by target reversal and 2 additional days of training. These groups of Sprague-Dawley male rats were used: TBI treated with PHY at 3.2 μmol/kg/day (TBI-PHY3.2), or 6.4 μmol/kg/day (TBI-PHY6.4), by subcutaneous osmotic pumps, or TBI and no injury (Sham) treated with saline. AChE activity was measured in brain tissue samples of non-traumatized animals that received PHY at the doses used in the TBI animals. In WM1 tests, PHY3.2 improved learning within sessions, but not between sessions, in the recall of the target position, while PHY6.4 had no significant effects. In WM2 tests, PHY improved within- and between-sessions performance at both dose levels. We found that continuous AChE inhibition interacted with repeated training on the water maze task to completely reverse the deficits seen in learning and memory induced by TBI. The PHY treatment also reduced the amount of brain tissue loss as measured using cresyl violet staining. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/197514 Scremin, Oscar Umberto; Norman, Keith M.; Roch, Margareth; Holschneider, Daniel P.; Scremin, A. M. Erika; Acetylcholinesterase inhibition interacts with training to reverse spatial learning deficits after cortical impact injury; Mary Ann Liebert; Journal of Neurotrauma; 29; 15; 10-2012; 2457-2464 0897-7151 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/197514 |
identifier_str_mv |
Scremin, Oscar Umberto; Norman, Keith M.; Roch, Margareth; Holschneider, Daniel P.; Scremin, A. M. Erika; Acetylcholinesterase inhibition interacts with training to reverse spatial learning deficits after cortical impact injury; Mary Ann Liebert; Journal of Neurotrauma; 29; 15; 10-2012; 2457-2464 0897-7151 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.liebertpub.com/doi/10.1089/neu.2012.2465 info:eu-repo/semantics/altIdentifier/doi/10.1089/neu.2012.2465 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Mary Ann Liebert |
publisher.none.fl_str_mv |
Mary Ann Liebert |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614432434421760 |
score |
13.070432 |