Acetylcholinesterase inhibition interacts with training to reverse spatial learning deficits after cortical impact injury

Autores
Scremin, Oscar Umberto; Norman, Keith M.; Roch, Margareth; Holschneider, Daniel P.; Scremin, A. M. Erika
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cholinergic mechanisms are known to play a key role in cognitive functions that are profoundly altered in traumatic brain injury (TBI). The present investigation was designed to test the ability of continuous administration, starting at the time of injury, of physostigmine (PHY), an acetylcholinesterase (AChE) inhibitor that crosses the blood-brain barrier (BBB), to ameliorate the alterations of learning and memory induced by cerebral cortex impact injury in rats under isoflurane anesthesia. Learning and memory were assessed with the Morris water maze implemented during days 7-11 (WM1), and days 21-25 post-TBI (WM2), with four trials per day for 3 days, followed by target reversal and 2 additional days of training. These groups of Sprague-Dawley male rats were used: TBI treated with PHY at 3.2 μmol/kg/day (TBI-PHY3.2), or 6.4 μmol/kg/day (TBI-PHY6.4), by subcutaneous osmotic pumps, or TBI and no injury (Sham) treated with saline. AChE activity was measured in brain tissue samples of non-traumatized animals that received PHY at the doses used in the TBI animals. In WM1 tests, PHY3.2 improved learning within sessions, but not between sessions, in the recall of the target position, while PHY6.4 had no significant effects. In WM2 tests, PHY improved within- and between-sessions performance at both dose levels. We found that continuous AChE inhibition interacted with repeated training on the water maze task to completely reverse the deficits seen in learning and memory induced by TBI. The PHY treatment also reduced the amount of brain tissue loss as measured using cresyl violet staining.
Fil: Scremin, Oscar Umberto. University of California at Los Angeles. School of Medicine; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Norman, Keith M.. No especifíca;
Fil: Roch, Margareth. No especifíca;
Fil: Holschneider, Daniel P.. No especifíca;
Fil: Scremin, A. M. Erika. University of California at Los Angeles. School of Medicine; Estados Unidos
Materia
ACETYLCHOLINESTERASE
CHOLINERGIC
NEUROREHABILITATION
SPATIAL LEARNING
TRAUMATIC BRAIN INJURY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/197514

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network_name_str CONICET Digital (CONICET)
spelling Acetylcholinesterase inhibition interacts with training to reverse spatial learning deficits after cortical impact injuryScremin, Oscar UmbertoNorman, Keith M.Roch, MargarethHolschneider, Daniel P.Scremin, A. M. ErikaACETYLCHOLINESTERASECHOLINERGICNEUROREHABILITATIONSPATIAL LEARNINGTRAUMATIC BRAIN INJURYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Cholinergic mechanisms are known to play a key role in cognitive functions that are profoundly altered in traumatic brain injury (TBI). The present investigation was designed to test the ability of continuous administration, starting at the time of injury, of physostigmine (PHY), an acetylcholinesterase (AChE) inhibitor that crosses the blood-brain barrier (BBB), to ameliorate the alterations of learning and memory induced by cerebral cortex impact injury in rats under isoflurane anesthesia. Learning and memory were assessed with the Morris water maze implemented during days 7-11 (WM1), and days 21-25 post-TBI (WM2), with four trials per day for 3 days, followed by target reversal and 2 additional days of training. These groups of Sprague-Dawley male rats were used: TBI treated with PHY at 3.2 μmol/kg/day (TBI-PHY3.2), or 6.4 μmol/kg/day (TBI-PHY6.4), by subcutaneous osmotic pumps, or TBI and no injury (Sham) treated with saline. AChE activity was measured in brain tissue samples of non-traumatized animals that received PHY at the doses used in the TBI animals. In WM1 tests, PHY3.2 improved learning within sessions, but not between sessions, in the recall of the target position, while PHY6.4 had no significant effects. In WM2 tests, PHY improved within- and between-sessions performance at both dose levels. We found that continuous AChE inhibition interacted with repeated training on the water maze task to completely reverse the deficits seen in learning and memory induced by TBI. The PHY treatment also reduced the amount of brain tissue loss as measured using cresyl violet staining.Fil: Scremin, Oscar Umberto. University of California at Los Angeles. School of Medicine; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFil: Norman, Keith M.. No especifíca;Fil: Roch, Margareth. No especifíca;Fil: Holschneider, Daniel P.. No especifíca;Fil: Scremin, A. M. Erika. University of California at Los Angeles. School of Medicine; Estados UnidosMary Ann Liebert2012-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/197514Scremin, Oscar Umberto; Norman, Keith M.; Roch, Margareth; Holschneider, Daniel P.; Scremin, A. M. Erika; Acetylcholinesterase inhibition interacts with training to reverse spatial learning deficits after cortical impact injury; Mary Ann Liebert; Journal of Neurotrauma; 29; 15; 10-2012; 2457-24640897-7151CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.liebertpub.com/doi/10.1089/neu.2012.2465info:eu-repo/semantics/altIdentifier/doi/10.1089/neu.2012.2465info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:40:27Zoai:ri.conicet.gov.ar:11336/197514instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:40:27.944CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Acetylcholinesterase inhibition interacts with training to reverse spatial learning deficits after cortical impact injury
title Acetylcholinesterase inhibition interacts with training to reverse spatial learning deficits after cortical impact injury
spellingShingle Acetylcholinesterase inhibition interacts with training to reverse spatial learning deficits after cortical impact injury
Scremin, Oscar Umberto
ACETYLCHOLINESTERASE
CHOLINERGIC
NEUROREHABILITATION
SPATIAL LEARNING
TRAUMATIC BRAIN INJURY
title_short Acetylcholinesterase inhibition interacts with training to reverse spatial learning deficits after cortical impact injury
title_full Acetylcholinesterase inhibition interacts with training to reverse spatial learning deficits after cortical impact injury
title_fullStr Acetylcholinesterase inhibition interacts with training to reverse spatial learning deficits after cortical impact injury
title_full_unstemmed Acetylcholinesterase inhibition interacts with training to reverse spatial learning deficits after cortical impact injury
title_sort Acetylcholinesterase inhibition interacts with training to reverse spatial learning deficits after cortical impact injury
dc.creator.none.fl_str_mv Scremin, Oscar Umberto
Norman, Keith M.
Roch, Margareth
Holschneider, Daniel P.
Scremin, A. M. Erika
author Scremin, Oscar Umberto
author_facet Scremin, Oscar Umberto
Norman, Keith M.
Roch, Margareth
Holschneider, Daniel P.
Scremin, A. M. Erika
author_role author
author2 Norman, Keith M.
Roch, Margareth
Holschneider, Daniel P.
Scremin, A. M. Erika
author2_role author
author
author
author
dc.subject.none.fl_str_mv ACETYLCHOLINESTERASE
CHOLINERGIC
NEUROREHABILITATION
SPATIAL LEARNING
TRAUMATIC BRAIN INJURY
topic ACETYLCHOLINESTERASE
CHOLINERGIC
NEUROREHABILITATION
SPATIAL LEARNING
TRAUMATIC BRAIN INJURY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Cholinergic mechanisms are known to play a key role in cognitive functions that are profoundly altered in traumatic brain injury (TBI). The present investigation was designed to test the ability of continuous administration, starting at the time of injury, of physostigmine (PHY), an acetylcholinesterase (AChE) inhibitor that crosses the blood-brain barrier (BBB), to ameliorate the alterations of learning and memory induced by cerebral cortex impact injury in rats under isoflurane anesthesia. Learning and memory were assessed with the Morris water maze implemented during days 7-11 (WM1), and days 21-25 post-TBI (WM2), with four trials per day for 3 days, followed by target reversal and 2 additional days of training. These groups of Sprague-Dawley male rats were used: TBI treated with PHY at 3.2 μmol/kg/day (TBI-PHY3.2), or 6.4 μmol/kg/day (TBI-PHY6.4), by subcutaneous osmotic pumps, or TBI and no injury (Sham) treated with saline. AChE activity was measured in brain tissue samples of non-traumatized animals that received PHY at the doses used in the TBI animals. In WM1 tests, PHY3.2 improved learning within sessions, but not between sessions, in the recall of the target position, while PHY6.4 had no significant effects. In WM2 tests, PHY improved within- and between-sessions performance at both dose levels. We found that continuous AChE inhibition interacted with repeated training on the water maze task to completely reverse the deficits seen in learning and memory induced by TBI. The PHY treatment also reduced the amount of brain tissue loss as measured using cresyl violet staining.
Fil: Scremin, Oscar Umberto. University of California at Los Angeles. School of Medicine; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Norman, Keith M.. No especifíca;
Fil: Roch, Margareth. No especifíca;
Fil: Holschneider, Daniel P.. No especifíca;
Fil: Scremin, A. M. Erika. University of California at Los Angeles. School of Medicine; Estados Unidos
description Cholinergic mechanisms are known to play a key role in cognitive functions that are profoundly altered in traumatic brain injury (TBI). The present investigation was designed to test the ability of continuous administration, starting at the time of injury, of physostigmine (PHY), an acetylcholinesterase (AChE) inhibitor that crosses the blood-brain barrier (BBB), to ameliorate the alterations of learning and memory induced by cerebral cortex impact injury in rats under isoflurane anesthesia. Learning and memory were assessed with the Morris water maze implemented during days 7-11 (WM1), and days 21-25 post-TBI (WM2), with four trials per day for 3 days, followed by target reversal and 2 additional days of training. These groups of Sprague-Dawley male rats were used: TBI treated with PHY at 3.2 μmol/kg/day (TBI-PHY3.2), or 6.4 μmol/kg/day (TBI-PHY6.4), by subcutaneous osmotic pumps, or TBI and no injury (Sham) treated with saline. AChE activity was measured in brain tissue samples of non-traumatized animals that received PHY at the doses used in the TBI animals. In WM1 tests, PHY3.2 improved learning within sessions, but not between sessions, in the recall of the target position, while PHY6.4 had no significant effects. In WM2 tests, PHY improved within- and between-sessions performance at both dose levels. We found that continuous AChE inhibition interacted with repeated training on the water maze task to completely reverse the deficits seen in learning and memory induced by TBI. The PHY treatment also reduced the amount of brain tissue loss as measured using cresyl violet staining.
publishDate 2012
dc.date.none.fl_str_mv 2012-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/197514
Scremin, Oscar Umberto; Norman, Keith M.; Roch, Margareth; Holschneider, Daniel P.; Scremin, A. M. Erika; Acetylcholinesterase inhibition interacts with training to reverse spatial learning deficits after cortical impact injury; Mary Ann Liebert; Journal of Neurotrauma; 29; 15; 10-2012; 2457-2464
0897-7151
CONICET Digital
CONICET
url http://hdl.handle.net/11336/197514
identifier_str_mv Scremin, Oscar Umberto; Norman, Keith M.; Roch, Margareth; Holschneider, Daniel P.; Scremin, A. M. Erika; Acetylcholinesterase inhibition interacts with training to reverse spatial learning deficits after cortical impact injury; Mary Ann Liebert; Journal of Neurotrauma; 29; 15; 10-2012; 2457-2464
0897-7151
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.liebertpub.com/doi/10.1089/neu.2012.2465
info:eu-repo/semantics/altIdentifier/doi/10.1089/neu.2012.2465
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Mary Ann Liebert
publisher.none.fl_str_mv Mary Ann Liebert
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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