Fabrication of a novel naltrexone biosensor based on a computationally engineered nanobiocomposite

Autores
Gholivand, Mohammad Bagher; Jalalvand, Ali R.; Paimard, Giti; Goicoechea, Hector Casimiro; Skov, Thomas; Farhadi, Reza; Ghobadi, Sirous; Moradi, Nozar; Nasirian, Vahid
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A computationally engineered impedimetric naltrexone (NLT) biosensor based on immobilization of bovine serum albumin (BSA) onto fullerene-C60/glassy carbon electrode (FLR/GCE) has been developed using initial characterization by computational methods and complementing them by experimental ones. Computational results showed that BSA hydrophobically binds to FLR which is energetically favorable and leads to the spontaneous formation of the stable nanobiocomposite and also showed that interaction of NLT with BSA is mainly driven by hydrogen bonding and hydrophobic interactions. Besides complementing the computational studies, experimental results showed that addition of FLR to the surface of the electrode facilitated electron transfer reactions, and also showed that the presence of BSA inhibits the interfacial electron transfer in some extent due to the nonconductive properties of BSA. The presence of NLT may form a negatively charged electroactive complex with BSA which repels the negatively charged redox probe and decelerates interfacial electron transfer leading to obvious faradaic impedance change. The faradaic impedance responses were linearly related to naltrexone concentration between 0.1 nM and 80 nM and limit of detection (LOD) was calculated to be 0.01 nM (3Sb/b). Finally, the proposed biosensor was successfully applied to determination of NLT in urine samples of both healthy and addict volunteers.
Fil: Gholivand, Mohammad Bagher. Razi University. Faculty of Chemistry; Irán
Fil: Jalalvand, Ali R.. Razi University. Faculty of Chemistry; Irán. Universidad Nacional del Litoral; Argentina
Fil: Paimard, Giti. Razi University. Faculty of Chemistry; Irán
Fil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral; Argentina
Fil: Skov, Thomas. Universidad de Copenhagen; Dinamarca
Fil: Farhadi, Reza. Razi University. Faculty of Chemistry; Irán
Fil: Ghobadi, Sirous. Razi University. Faculty of Science. Department of Biology; Irán
Fil: Moradi, Nozar. Razi University. Faculty of Chemistry; Irán
Fil: Nasirian, Vahid. Razi University. Faculty of Chemistry; Irán
Materia
Biosensor
Bovine Serum Albumin
Naltrexone
Fullerene-C60
Computational And Experimental Characterizations
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/16035
Acceder
id CONICETDig_adaed22ade3548cc5fd876e88eea48f6
oai_identifier_str oai:ri.conicet.gov.ar:11336/16035
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Fabrication of a novel naltrexone biosensor based on a computationally engineered nanobiocompositeGholivand, Mohammad BagherJalalvand, Ali R.Paimard, GitiGoicoechea, Hector CasimiroSkov, ThomasFarhadi, RezaGhobadi, SirousMoradi, NozarNasirian, VahidBiosensorBovine Serum AlbuminNaltrexoneFullerene-C60Computational And Experimental Characterizationshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A computationally engineered impedimetric naltrexone (NLT) biosensor based on immobilization of bovine serum albumin (BSA) onto fullerene-C60/glassy carbon electrode (FLR/GCE) has been developed using initial characterization by computational methods and complementing them by experimental ones. Computational results showed that BSA hydrophobically binds to FLR which is energetically favorable and leads to the spontaneous formation of the stable nanobiocomposite and also showed that interaction of NLT with BSA is mainly driven by hydrogen bonding and hydrophobic interactions. Besides complementing the computational studies, experimental results showed that addition of FLR to the surface of the electrode facilitated electron transfer reactions, and also showed that the presence of BSA inhibits the interfacial electron transfer in some extent due to the nonconductive properties of BSA. The presence of NLT may form a negatively charged electroactive complex with BSA which repels the negatively charged redox probe and decelerates interfacial electron transfer leading to obvious faradaic impedance change. The faradaic impedance responses were linearly related to naltrexone concentration between 0.1 nM and 80 nM and limit of detection (LOD) was calculated to be 0.01 nM (3Sb/b). Finally, the proposed biosensor was successfully applied to determination of NLT in urine samples of both healthy and addict volunteers.Fil: Gholivand, Mohammad Bagher. Razi University. Faculty of Chemistry; IránFil: Jalalvand, Ali R.. Razi University. Faculty of Chemistry; Irán. Universidad Nacional del Litoral; ArgentinaFil: Paimard, Giti. Razi University. Faculty of Chemistry; IránFil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral; ArgentinaFil: Skov, Thomas. Universidad de Copenhagen; DinamarcaFil: Farhadi, Reza. Razi University. Faculty of Chemistry; IránFil: Ghobadi, Sirous. Razi University. Faculty of Science. Department of Biology; IránFil: Moradi, Nozar. Razi University. Faculty of Chemistry; IránFil: Nasirian, Vahid. Razi University. Faculty of Chemistry; IránElsevier Science2014-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16035Gholivand, Mohammad Bagher; Jalalvand, Ali R.; Paimard, Giti; Goicoechea, Hector Casimiro; Skov, Thomas; et al.; Fabrication of a novel naltrexone biosensor based on a computationally engineered nanobiocomposite; Elsevier Science; International Journal of Biological Macromolecules; 70; 9-2014; 596-6050141-8130enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijbiomac.2014.07.028info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0141813014005030info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:22:31Zoai:ri.conicet.gov.ar:11336/16035instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:22:31.391CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Fabrication of a novel naltrexone biosensor based on a computationally engineered nanobiocomposite
title Fabrication of a novel naltrexone biosensor based on a computationally engineered nanobiocomposite
spellingShingle Fabrication of a novel naltrexone biosensor based on a computationally engineered nanobiocomposite
Gholivand, Mohammad Bagher
Biosensor
Bovine Serum Albumin
Naltrexone
Fullerene-C60
Computational And Experimental Characterizations
title_short Fabrication of a novel naltrexone biosensor based on a computationally engineered nanobiocomposite
title_full Fabrication of a novel naltrexone biosensor based on a computationally engineered nanobiocomposite
title_fullStr Fabrication of a novel naltrexone biosensor based on a computationally engineered nanobiocomposite
title_full_unstemmed Fabrication of a novel naltrexone biosensor based on a computationally engineered nanobiocomposite
title_sort Fabrication of a novel naltrexone biosensor based on a computationally engineered nanobiocomposite
dc.creator.none.fl_str_mv Gholivand, Mohammad Bagher
Jalalvand, Ali R.
Paimard, Giti
Goicoechea, Hector Casimiro
Skov, Thomas
Farhadi, Reza
Ghobadi, Sirous
Moradi, Nozar
Nasirian, Vahid
author Gholivand, Mohammad Bagher
author_facet Gholivand, Mohammad Bagher
Jalalvand, Ali R.
Paimard, Giti
Goicoechea, Hector Casimiro
Skov, Thomas
Farhadi, Reza
Ghobadi, Sirous
Moradi, Nozar
Nasirian, Vahid
author_role author
author2 Jalalvand, Ali R.
Paimard, Giti
Goicoechea, Hector Casimiro
Skov, Thomas
Farhadi, Reza
Ghobadi, Sirous
Moradi, Nozar
Nasirian, Vahid
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Biosensor
Bovine Serum Albumin
Naltrexone
Fullerene-C60
Computational And Experimental Characterizations
topic Biosensor
Bovine Serum Albumin
Naltrexone
Fullerene-C60
Computational And Experimental Characterizations
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A computationally engineered impedimetric naltrexone (NLT) biosensor based on immobilization of bovine serum albumin (BSA) onto fullerene-C60/glassy carbon electrode (FLR/GCE) has been developed using initial characterization by computational methods and complementing them by experimental ones. Computational results showed that BSA hydrophobically binds to FLR which is energetically favorable and leads to the spontaneous formation of the stable nanobiocomposite and also showed that interaction of NLT with BSA is mainly driven by hydrogen bonding and hydrophobic interactions. Besides complementing the computational studies, experimental results showed that addition of FLR to the surface of the electrode facilitated electron transfer reactions, and also showed that the presence of BSA inhibits the interfacial electron transfer in some extent due to the nonconductive properties of BSA. The presence of NLT may form a negatively charged electroactive complex with BSA which repels the negatively charged redox probe and decelerates interfacial electron transfer leading to obvious faradaic impedance change. The faradaic impedance responses were linearly related to naltrexone concentration between 0.1 nM and 80 nM and limit of detection (LOD) was calculated to be 0.01 nM (3Sb/b). Finally, the proposed biosensor was successfully applied to determination of NLT in urine samples of both healthy and addict volunteers.
Fil: Gholivand, Mohammad Bagher. Razi University. Faculty of Chemistry; Irán
Fil: Jalalvand, Ali R.. Razi University. Faculty of Chemistry; Irán. Universidad Nacional del Litoral; Argentina
Fil: Paimard, Giti. Razi University. Faculty of Chemistry; Irán
Fil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral; Argentina
Fil: Skov, Thomas. Universidad de Copenhagen; Dinamarca
Fil: Farhadi, Reza. Razi University. Faculty of Chemistry; Irán
Fil: Ghobadi, Sirous. Razi University. Faculty of Science. Department of Biology; Irán
Fil: Moradi, Nozar. Razi University. Faculty of Chemistry; Irán
Fil: Nasirian, Vahid. Razi University. Faculty of Chemistry; Irán
description A computationally engineered impedimetric naltrexone (NLT) biosensor based on immobilization of bovine serum albumin (BSA) onto fullerene-C60/glassy carbon electrode (FLR/GCE) has been developed using initial characterization by computational methods and complementing them by experimental ones. Computational results showed that BSA hydrophobically binds to FLR which is energetically favorable and leads to the spontaneous formation of the stable nanobiocomposite and also showed that interaction of NLT with BSA is mainly driven by hydrogen bonding and hydrophobic interactions. Besides complementing the computational studies, experimental results showed that addition of FLR to the surface of the electrode facilitated electron transfer reactions, and also showed that the presence of BSA inhibits the interfacial electron transfer in some extent due to the nonconductive properties of BSA. The presence of NLT may form a negatively charged electroactive complex with BSA which repels the negatively charged redox probe and decelerates interfacial electron transfer leading to obvious faradaic impedance change. The faradaic impedance responses were linearly related to naltrexone concentration between 0.1 nM and 80 nM and limit of detection (LOD) was calculated to be 0.01 nM (3Sb/b). Finally, the proposed biosensor was successfully applied to determination of NLT in urine samples of both healthy and addict volunteers.
publishDate 2014
dc.date.none.fl_str_mv 2014-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/16035
Gholivand, Mohammad Bagher; Jalalvand, Ali R.; Paimard, Giti; Goicoechea, Hector Casimiro; Skov, Thomas; et al.; Fabrication of a novel naltrexone biosensor based on a computationally engineered nanobiocomposite; Elsevier Science; International Journal of Biological Macromolecules; 70; 9-2014; 596-605
0141-8130
url http://hdl.handle.net/11336/16035
identifier_str_mv Gholivand, Mohammad Bagher; Jalalvand, Ali R.; Paimard, Giti; Goicoechea, Hector Casimiro; Skov, Thomas; et al.; Fabrication of a novel naltrexone biosensor based on a computationally engineered nanobiocomposite; Elsevier Science; International Journal of Biological Macromolecules; 70; 9-2014; 596-605
0141-8130
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijbiomac.2014.07.028
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0141813014005030
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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