Fabrication of a novel naltrexone biosensor based on a computationally engineered nanobiocomposite
- Autores
- Gholivand, Mohammad Bagher; Jalalvand, Ali R.; Paimard, Giti; Goicoechea, Hector Casimiro; Skov, Thomas; Farhadi, Reza; Ghobadi, Sirous; Moradi, Nozar; Nasirian, Vahid
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- A computationally engineered impedimetric naltrexone (NLT) biosensor based on immobilization of bovine serum albumin (BSA) onto fullerene-C60/glassy carbon electrode (FLR/GCE) has been developed using initial characterization by computational methods and complementing them by experimental ones. Computational results showed that BSA hydrophobically binds to FLR which is energetically favorable and leads to the spontaneous formation of the stable nanobiocomposite and also showed that interaction of NLT with BSA is mainly driven by hydrogen bonding and hydrophobic interactions. Besides complementing the computational studies, experimental results showed that addition of FLR to the surface of the electrode facilitated electron transfer reactions, and also showed that the presence of BSA inhibits the interfacial electron transfer in some extent due to the nonconductive properties of BSA. The presence of NLT may form a negatively charged electroactive complex with BSA which repels the negatively charged redox probe and decelerates interfacial electron transfer leading to obvious faradaic impedance change. The faradaic impedance responses were linearly related to naltrexone concentration between 0.1 nM and 80 nM and limit of detection (LOD) was calculated to be 0.01 nM (3Sb/b). Finally, the proposed biosensor was successfully applied to determination of NLT in urine samples of both healthy and addict volunteers.
Fil: Gholivand, Mohammad Bagher. Razi University. Faculty of Chemistry; Irán
Fil: Jalalvand, Ali R.. Razi University. Faculty of Chemistry; Irán. Universidad Nacional del Litoral; Argentina
Fil: Paimard, Giti. Razi University. Faculty of Chemistry; Irán
Fil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral; Argentina
Fil: Skov, Thomas. Universidad de Copenhagen; Dinamarca
Fil: Farhadi, Reza. Razi University. Faculty of Chemistry; Irán
Fil: Ghobadi, Sirous. Razi University. Faculty of Science. Department of Biology; Irán
Fil: Moradi, Nozar. Razi University. Faculty of Chemistry; Irán
Fil: Nasirian, Vahid. Razi University. Faculty of Chemistry; Irán - Materia
-
Biosensor
Bovine Serum Albumin
Naltrexone
Fullerene-C60
Computational And Experimental Characterizations - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/16035
Ver los metadatos del registro completo
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Fabrication of a novel naltrexone biosensor based on a computationally engineered nanobiocompositeGholivand, Mohammad BagherJalalvand, Ali R.Paimard, GitiGoicoechea, Hector CasimiroSkov, ThomasFarhadi, RezaGhobadi, SirousMoradi, NozarNasirian, VahidBiosensorBovine Serum AlbuminNaltrexoneFullerene-C60Computational And Experimental Characterizationshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A computationally engineered impedimetric naltrexone (NLT) biosensor based on immobilization of bovine serum albumin (BSA) onto fullerene-C60/glassy carbon electrode (FLR/GCE) has been developed using initial characterization by computational methods and complementing them by experimental ones. Computational results showed that BSA hydrophobically binds to FLR which is energetically favorable and leads to the spontaneous formation of the stable nanobiocomposite and also showed that interaction of NLT with BSA is mainly driven by hydrogen bonding and hydrophobic interactions. Besides complementing the computational studies, experimental results showed that addition of FLR to the surface of the electrode facilitated electron transfer reactions, and also showed that the presence of BSA inhibits the interfacial electron transfer in some extent due to the nonconductive properties of BSA. The presence of NLT may form a negatively charged electroactive complex with BSA which repels the negatively charged redox probe and decelerates interfacial electron transfer leading to obvious faradaic impedance change. The faradaic impedance responses were linearly related to naltrexone concentration between 0.1 nM and 80 nM and limit of detection (LOD) was calculated to be 0.01 nM (3Sb/b). Finally, the proposed biosensor was successfully applied to determination of NLT in urine samples of both healthy and addict volunteers.Fil: Gholivand, Mohammad Bagher. Razi University. Faculty of Chemistry; IránFil: Jalalvand, Ali R.. Razi University. Faculty of Chemistry; Irán. Universidad Nacional del Litoral; ArgentinaFil: Paimard, Giti. Razi University. Faculty of Chemistry; IránFil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral; ArgentinaFil: Skov, Thomas. Universidad de Copenhagen; DinamarcaFil: Farhadi, Reza. Razi University. Faculty of Chemistry; IránFil: Ghobadi, Sirous. Razi University. Faculty of Science. Department of Biology; IránFil: Moradi, Nozar. Razi University. Faculty of Chemistry; IránFil: Nasirian, Vahid. Razi University. Faculty of Chemistry; IránElsevier Science2014-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16035Gholivand, Mohammad Bagher; Jalalvand, Ali R.; Paimard, Giti; Goicoechea, Hector Casimiro; Skov, Thomas; et al.; Fabrication of a novel naltrexone biosensor based on a computationally engineered nanobiocomposite; Elsevier Science; International Journal of Biological Macromolecules; 70; 9-2014; 596-6050141-8130enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijbiomac.2014.07.028info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0141813014005030info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:34:15Zoai:ri.conicet.gov.ar:11336/16035instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:34:15.343CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Fabrication of a novel naltrexone biosensor based on a computationally engineered nanobiocomposite |
title |
Fabrication of a novel naltrexone biosensor based on a computationally engineered nanobiocomposite |
spellingShingle |
Fabrication of a novel naltrexone biosensor based on a computationally engineered nanobiocomposite Gholivand, Mohammad Bagher Biosensor Bovine Serum Albumin Naltrexone Fullerene-C60 Computational And Experimental Characterizations |
title_short |
Fabrication of a novel naltrexone biosensor based on a computationally engineered nanobiocomposite |
title_full |
Fabrication of a novel naltrexone biosensor based on a computationally engineered nanobiocomposite |
title_fullStr |
Fabrication of a novel naltrexone biosensor based on a computationally engineered nanobiocomposite |
title_full_unstemmed |
Fabrication of a novel naltrexone biosensor based on a computationally engineered nanobiocomposite |
title_sort |
Fabrication of a novel naltrexone biosensor based on a computationally engineered nanobiocomposite |
dc.creator.none.fl_str_mv |
Gholivand, Mohammad Bagher Jalalvand, Ali R. Paimard, Giti Goicoechea, Hector Casimiro Skov, Thomas Farhadi, Reza Ghobadi, Sirous Moradi, Nozar Nasirian, Vahid |
author |
Gholivand, Mohammad Bagher |
author_facet |
Gholivand, Mohammad Bagher Jalalvand, Ali R. Paimard, Giti Goicoechea, Hector Casimiro Skov, Thomas Farhadi, Reza Ghobadi, Sirous Moradi, Nozar Nasirian, Vahid |
author_role |
author |
author2 |
Jalalvand, Ali R. Paimard, Giti Goicoechea, Hector Casimiro Skov, Thomas Farhadi, Reza Ghobadi, Sirous Moradi, Nozar Nasirian, Vahid |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
Biosensor Bovine Serum Albumin Naltrexone Fullerene-C60 Computational And Experimental Characterizations |
topic |
Biosensor Bovine Serum Albumin Naltrexone Fullerene-C60 Computational And Experimental Characterizations |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
A computationally engineered impedimetric naltrexone (NLT) biosensor based on immobilization of bovine serum albumin (BSA) onto fullerene-C60/glassy carbon electrode (FLR/GCE) has been developed using initial characterization by computational methods and complementing them by experimental ones. Computational results showed that BSA hydrophobically binds to FLR which is energetically favorable and leads to the spontaneous formation of the stable nanobiocomposite and also showed that interaction of NLT with BSA is mainly driven by hydrogen bonding and hydrophobic interactions. Besides complementing the computational studies, experimental results showed that addition of FLR to the surface of the electrode facilitated electron transfer reactions, and also showed that the presence of BSA inhibits the interfacial electron transfer in some extent due to the nonconductive properties of BSA. The presence of NLT may form a negatively charged electroactive complex with BSA which repels the negatively charged redox probe and decelerates interfacial electron transfer leading to obvious faradaic impedance change. The faradaic impedance responses were linearly related to naltrexone concentration between 0.1 nM and 80 nM and limit of detection (LOD) was calculated to be 0.01 nM (3Sb/b). Finally, the proposed biosensor was successfully applied to determination of NLT in urine samples of both healthy and addict volunteers. Fil: Gholivand, Mohammad Bagher. Razi University. Faculty of Chemistry; Irán Fil: Jalalvand, Ali R.. Razi University. Faculty of Chemistry; Irán. Universidad Nacional del Litoral; Argentina Fil: Paimard, Giti. Razi University. Faculty of Chemistry; Irán Fil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral; Argentina Fil: Skov, Thomas. Universidad de Copenhagen; Dinamarca Fil: Farhadi, Reza. Razi University. Faculty of Chemistry; Irán Fil: Ghobadi, Sirous. Razi University. Faculty of Science. Department of Biology; Irán Fil: Moradi, Nozar. Razi University. Faculty of Chemistry; Irán Fil: Nasirian, Vahid. Razi University. Faculty of Chemistry; Irán |
description |
A computationally engineered impedimetric naltrexone (NLT) biosensor based on immobilization of bovine serum albumin (BSA) onto fullerene-C60/glassy carbon electrode (FLR/GCE) has been developed using initial characterization by computational methods and complementing them by experimental ones. Computational results showed that BSA hydrophobically binds to FLR which is energetically favorable and leads to the spontaneous formation of the stable nanobiocomposite and also showed that interaction of NLT with BSA is mainly driven by hydrogen bonding and hydrophobic interactions. Besides complementing the computational studies, experimental results showed that addition of FLR to the surface of the electrode facilitated electron transfer reactions, and also showed that the presence of BSA inhibits the interfacial electron transfer in some extent due to the nonconductive properties of BSA. The presence of NLT may form a negatively charged electroactive complex with BSA which repels the negatively charged redox probe and decelerates interfacial electron transfer leading to obvious faradaic impedance change. The faradaic impedance responses were linearly related to naltrexone concentration between 0.1 nM and 80 nM and limit of detection (LOD) was calculated to be 0.01 nM (3Sb/b). Finally, the proposed biosensor was successfully applied to determination of NLT in urine samples of both healthy and addict volunteers. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-09 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/16035 Gholivand, Mohammad Bagher; Jalalvand, Ali R.; Paimard, Giti; Goicoechea, Hector Casimiro; Skov, Thomas; et al.; Fabrication of a novel naltrexone biosensor based on a computationally engineered nanobiocomposite; Elsevier Science; International Journal of Biological Macromolecules; 70; 9-2014; 596-605 0141-8130 |
url |
http://hdl.handle.net/11336/16035 |
identifier_str_mv |
Gholivand, Mohammad Bagher; Jalalvand, Ali R.; Paimard, Giti; Goicoechea, Hector Casimiro; Skov, Thomas; et al.; Fabrication of a novel naltrexone biosensor based on a computationally engineered nanobiocomposite; Elsevier Science; International Journal of Biological Macromolecules; 70; 9-2014; 596-605 0141-8130 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijbiomac.2014.07.028 info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0141813014005030 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science |
publisher.none.fl_str_mv |
Elsevier Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613058724364288 |
score |
13.070432 |