Developing a novel computationally designed impedimetric pregabalin biosensor

Autores
Gholivand, Mohammad Bagher; Jalalvand, Ali R.; Goicoechea, Hector Casimiro
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A computationally designed impedimetric pregabalin (PGB) biosensor based on immobilization of bovine serum albumin (BSA) onto graphene/glassy carbon electrode (BSA/Gr/GCE) has been developed using initial characterization by computational methods and complementing them by experimental observations. Computational results showed that the BSA hydrophobically binds to Gr which is energetically favorable and leads to the spontaneous formation of the stable nanobiocomposite (BSA/Gr), and also showed that the interaction of PGB with BSA is mainly driven by hydrogen bonding and hydrophobic interactions. The interactions of BSA with Gr and PGB with BSA were also monitored by fluorescence and UVvis spectroscopic techniques, and their results were consistent with the computational results. The electrochemical properties of the fabricated composite electrodes were examined by cyclic voltammetry (CV) scanning electron microscopy (SEM), and electrochemical impedance spectroscopy (EIS) techniques. Besides complementing the computational studies, experimental results showed that the addition of Gr to the surface of the electrode facilitated the electron transfer reactions, and also showed that the presence of BSA inhibits the interfacial electron transfer in some extent due to the non-conductive properties of BSA. The presence of the PGB may form an electroinactive complex with BSA which decelerates the interfacial electron transfer leading to obvious faradaic impedance changes. The faradaic impedance responses were linearly related to PGB concentration between 10.0 nM and 280.0 nM and the limit of detection (LOD) was calculated to be 3.0 nM (3Sb/b). Finally, the proposed biosensor was successfully applied to determination of PGB in human serum samples. The results were satisfactory and comparable to those obtained by applying the reference method based on high performance liquid chromatography (HPLC). The results confirmed that the proposed biosensor has good sensitivity, selectivity, stability, repeatability, reproducibility, and regeneration ability for PGB determination.
Fil: Gholivand, Mohammad Bagher. Razi University. Faculty of Chemistry; Irán
Fil: Jalalvand, Ali R.. Razi University. Faculty of Chemistry; Irán. Universidad Nacional del Litoral. Cátedra de Química Analítica I. Laboratorio de Desarrollo Analítico y Quimiometría; Argentina
Fil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral. Cátedra de Química Analítica I. Laboratorio de Desarrollo Analítico y Quimiometría; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe; Argentina
Materia
Pregabalin
Bovine Serum Albumin
Graphene
Computationally Designed Biosensor
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/15365

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spelling Developing a novel computationally designed impedimetric pregabalin biosensorGholivand, Mohammad BagherJalalvand, Ali R.Goicoechea, Hector CasimiroPregabalinBovine Serum AlbuminGrapheneComputationally Designed Biosensorhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A computationally designed impedimetric pregabalin (PGB) biosensor based on immobilization of bovine serum albumin (BSA) onto graphene/glassy carbon electrode (BSA/Gr/GCE) has been developed using initial characterization by computational methods and complementing them by experimental observations. Computational results showed that the BSA hydrophobically binds to Gr which is energetically favorable and leads to the spontaneous formation of the stable nanobiocomposite (BSA/Gr), and also showed that the interaction of PGB with BSA is mainly driven by hydrogen bonding and hydrophobic interactions. The interactions of BSA with Gr and PGB with BSA were also monitored by fluorescence and UVvis spectroscopic techniques, and their results were consistent with the computational results. The electrochemical properties of the fabricated composite electrodes were examined by cyclic voltammetry (CV) scanning electron microscopy (SEM), and electrochemical impedance spectroscopy (EIS) techniques. Besides complementing the computational studies, experimental results showed that the addition of Gr to the surface of the electrode facilitated the electron transfer reactions, and also showed that the presence of BSA inhibits the interfacial electron transfer in some extent due to the non-conductive properties of BSA. The presence of the PGB may form an electroinactive complex with BSA which decelerates the interfacial electron transfer leading to obvious faradaic impedance changes. The faradaic impedance responses were linearly related to PGB concentration between 10.0 nM and 280.0 nM and the limit of detection (LOD) was calculated to be 3.0 nM (3Sb/b). Finally, the proposed biosensor was successfully applied to determination of PGB in human serum samples. The results were satisfactory and comparable to those obtained by applying the reference method based on high performance liquid chromatography (HPLC). The results confirmed that the proposed biosensor has good sensitivity, selectivity, stability, repeatability, reproducibility, and regeneration ability for PGB determination.Fil: Gholivand, Mohammad Bagher. Razi University. Faculty of Chemistry; IránFil: Jalalvand, Ali R.. Razi University. Faculty of Chemistry; Irán. Universidad Nacional del Litoral. Cátedra de Química Analítica I. Laboratorio de Desarrollo Analítico y Quimiometría; ArgentinaFil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral. Cátedra de Química Analítica I. Laboratorio de Desarrollo Analítico y Quimiometría; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe; ArgentinaElsevier2014-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/15365Gholivand, Mohammad Bagher; Jalalvand, Ali R.; Goicoechea, Hector Casimiro; Developing a novel computationally designed impedimetric pregabalin biosensor; Elsevier; Electrochimica Acta; 133; 6-2014; 123-1310013-4686enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.electacta.2014.04.017info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S001346861400752Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:41:41Zoai:ri.conicet.gov.ar:11336/15365instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:41:42.105CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Developing a novel computationally designed impedimetric pregabalin biosensor
title Developing a novel computationally designed impedimetric pregabalin biosensor
spellingShingle Developing a novel computationally designed impedimetric pregabalin biosensor
Gholivand, Mohammad Bagher
Pregabalin
Bovine Serum Albumin
Graphene
Computationally Designed Biosensor
title_short Developing a novel computationally designed impedimetric pregabalin biosensor
title_full Developing a novel computationally designed impedimetric pregabalin biosensor
title_fullStr Developing a novel computationally designed impedimetric pregabalin biosensor
title_full_unstemmed Developing a novel computationally designed impedimetric pregabalin biosensor
title_sort Developing a novel computationally designed impedimetric pregabalin biosensor
dc.creator.none.fl_str_mv Gholivand, Mohammad Bagher
Jalalvand, Ali R.
Goicoechea, Hector Casimiro
author Gholivand, Mohammad Bagher
author_facet Gholivand, Mohammad Bagher
Jalalvand, Ali R.
Goicoechea, Hector Casimiro
author_role author
author2 Jalalvand, Ali R.
Goicoechea, Hector Casimiro
author2_role author
author
dc.subject.none.fl_str_mv Pregabalin
Bovine Serum Albumin
Graphene
Computationally Designed Biosensor
topic Pregabalin
Bovine Serum Albumin
Graphene
Computationally Designed Biosensor
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A computationally designed impedimetric pregabalin (PGB) biosensor based on immobilization of bovine serum albumin (BSA) onto graphene/glassy carbon electrode (BSA/Gr/GCE) has been developed using initial characterization by computational methods and complementing them by experimental observations. Computational results showed that the BSA hydrophobically binds to Gr which is energetically favorable and leads to the spontaneous formation of the stable nanobiocomposite (BSA/Gr), and also showed that the interaction of PGB with BSA is mainly driven by hydrogen bonding and hydrophobic interactions. The interactions of BSA with Gr and PGB with BSA were also monitored by fluorescence and UVvis spectroscopic techniques, and their results were consistent with the computational results. The electrochemical properties of the fabricated composite electrodes were examined by cyclic voltammetry (CV) scanning electron microscopy (SEM), and electrochemical impedance spectroscopy (EIS) techniques. Besides complementing the computational studies, experimental results showed that the addition of Gr to the surface of the electrode facilitated the electron transfer reactions, and also showed that the presence of BSA inhibits the interfacial electron transfer in some extent due to the non-conductive properties of BSA. The presence of the PGB may form an electroinactive complex with BSA which decelerates the interfacial electron transfer leading to obvious faradaic impedance changes. The faradaic impedance responses were linearly related to PGB concentration between 10.0 nM and 280.0 nM and the limit of detection (LOD) was calculated to be 3.0 nM (3Sb/b). Finally, the proposed biosensor was successfully applied to determination of PGB in human serum samples. The results were satisfactory and comparable to those obtained by applying the reference method based on high performance liquid chromatography (HPLC). The results confirmed that the proposed biosensor has good sensitivity, selectivity, stability, repeatability, reproducibility, and regeneration ability for PGB determination.
Fil: Gholivand, Mohammad Bagher. Razi University. Faculty of Chemistry; Irán
Fil: Jalalvand, Ali R.. Razi University. Faculty of Chemistry; Irán. Universidad Nacional del Litoral. Cátedra de Química Analítica I. Laboratorio de Desarrollo Analítico y Quimiometría; Argentina
Fil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral. Cátedra de Química Analítica I. Laboratorio de Desarrollo Analítico y Quimiometría; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe; Argentina
description A computationally designed impedimetric pregabalin (PGB) biosensor based on immobilization of bovine serum albumin (BSA) onto graphene/glassy carbon electrode (BSA/Gr/GCE) has been developed using initial characterization by computational methods and complementing them by experimental observations. Computational results showed that the BSA hydrophobically binds to Gr which is energetically favorable and leads to the spontaneous formation of the stable nanobiocomposite (BSA/Gr), and also showed that the interaction of PGB with BSA is mainly driven by hydrogen bonding and hydrophobic interactions. The interactions of BSA with Gr and PGB with BSA were also monitored by fluorescence and UVvis spectroscopic techniques, and their results were consistent with the computational results. The electrochemical properties of the fabricated composite electrodes were examined by cyclic voltammetry (CV) scanning electron microscopy (SEM), and electrochemical impedance spectroscopy (EIS) techniques. Besides complementing the computational studies, experimental results showed that the addition of Gr to the surface of the electrode facilitated the electron transfer reactions, and also showed that the presence of BSA inhibits the interfacial electron transfer in some extent due to the non-conductive properties of BSA. The presence of the PGB may form an electroinactive complex with BSA which decelerates the interfacial electron transfer leading to obvious faradaic impedance changes. The faradaic impedance responses were linearly related to PGB concentration between 10.0 nM and 280.0 nM and the limit of detection (LOD) was calculated to be 3.0 nM (3Sb/b). Finally, the proposed biosensor was successfully applied to determination of PGB in human serum samples. The results were satisfactory and comparable to those obtained by applying the reference method based on high performance liquid chromatography (HPLC). The results confirmed that the proposed biosensor has good sensitivity, selectivity, stability, repeatability, reproducibility, and regeneration ability for PGB determination.
publishDate 2014
dc.date.none.fl_str_mv 2014-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/15365
Gholivand, Mohammad Bagher; Jalalvand, Ali R.; Goicoechea, Hector Casimiro; Developing a novel computationally designed impedimetric pregabalin biosensor; Elsevier; Electrochimica Acta; 133; 6-2014; 123-131
0013-4686
url http://hdl.handle.net/11336/15365
identifier_str_mv Gholivand, Mohammad Bagher; Jalalvand, Ali R.; Goicoechea, Hector Casimiro; Developing a novel computationally designed impedimetric pregabalin biosensor; Elsevier; Electrochimica Acta; 133; 6-2014; 123-131
0013-4686
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.electacta.2014.04.017
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S001346861400752X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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