Developing a novel computationally designed impedimetric pregabalin biosensor
- Autores
- Gholivand, Mohammad Bagher; Jalalvand, Ali R.; Goicoechea, Hector Casimiro
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- A computationally designed impedimetric pregabalin (PGB) biosensor based on immobilization of bovine serum albumin (BSA) onto graphene/glassy carbon electrode (BSA/Gr/GCE) has been developed using initial characterization by computational methods and complementing them by experimental observations. Computational results showed that the BSA hydrophobically binds to Gr which is energetically favorable and leads to the spontaneous formation of the stable nanobiocomposite (BSA/Gr), and also showed that the interaction of PGB with BSA is mainly driven by hydrogen bonding and hydrophobic interactions. The interactions of BSA with Gr and PGB with BSA were also monitored by fluorescence and UVvis spectroscopic techniques, and their results were consistent with the computational results. The electrochemical properties of the fabricated composite electrodes were examined by cyclic voltammetry (CV) scanning electron microscopy (SEM), and electrochemical impedance spectroscopy (EIS) techniques. Besides complementing the computational studies, experimental results showed that the addition of Gr to the surface of the electrode facilitated the electron transfer reactions, and also showed that the presence of BSA inhibits the interfacial electron transfer in some extent due to the non-conductive properties of BSA. The presence of the PGB may form an electroinactive complex with BSA which decelerates the interfacial electron transfer leading to obvious faradaic impedance changes. The faradaic impedance responses were linearly related to PGB concentration between 10.0 nM and 280.0 nM and the limit of detection (LOD) was calculated to be 3.0 nM (3Sb/b). Finally, the proposed biosensor was successfully applied to determination of PGB in human serum samples. The results were satisfactory and comparable to those obtained by applying the reference method based on high performance liquid chromatography (HPLC). The results confirmed that the proposed biosensor has good sensitivity, selectivity, stability, repeatability, reproducibility, and regeneration ability for PGB determination.
Fil: Gholivand, Mohammad Bagher. Razi University. Faculty of Chemistry; Irán
Fil: Jalalvand, Ali R.. Razi University. Faculty of Chemistry; Irán. Universidad Nacional del Litoral. Cátedra de Química Analítica I. Laboratorio de Desarrollo Analítico y Quimiometría; Argentina
Fil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral. Cátedra de Química Analítica I. Laboratorio de Desarrollo Analítico y Quimiometría; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe; Argentina - Materia
-
Pregabalin
Bovine Serum Albumin
Graphene
Computationally Designed Biosensor - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/15365
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Developing a novel computationally designed impedimetric pregabalin biosensorGholivand, Mohammad BagherJalalvand, Ali R.Goicoechea, Hector CasimiroPregabalinBovine Serum AlbuminGrapheneComputationally Designed Biosensorhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A computationally designed impedimetric pregabalin (PGB) biosensor based on immobilization of bovine serum albumin (BSA) onto graphene/glassy carbon electrode (BSA/Gr/GCE) has been developed using initial characterization by computational methods and complementing them by experimental observations. Computational results showed that the BSA hydrophobically binds to Gr which is energetically favorable and leads to the spontaneous formation of the stable nanobiocomposite (BSA/Gr), and also showed that the interaction of PGB with BSA is mainly driven by hydrogen bonding and hydrophobic interactions. The interactions of BSA with Gr and PGB with BSA were also monitored by fluorescence and UVvis spectroscopic techniques, and their results were consistent with the computational results. The electrochemical properties of the fabricated composite electrodes were examined by cyclic voltammetry (CV) scanning electron microscopy (SEM), and electrochemical impedance spectroscopy (EIS) techniques. Besides complementing the computational studies, experimental results showed that the addition of Gr to the surface of the electrode facilitated the electron transfer reactions, and also showed that the presence of BSA inhibits the interfacial electron transfer in some extent due to the non-conductive properties of BSA. The presence of the PGB may form an electroinactive complex with BSA which decelerates the interfacial electron transfer leading to obvious faradaic impedance changes. The faradaic impedance responses were linearly related to PGB concentration between 10.0 nM and 280.0 nM and the limit of detection (LOD) was calculated to be 3.0 nM (3Sb/b). Finally, the proposed biosensor was successfully applied to determination of PGB in human serum samples. The results were satisfactory and comparable to those obtained by applying the reference method based on high performance liquid chromatography (HPLC). The results confirmed that the proposed biosensor has good sensitivity, selectivity, stability, repeatability, reproducibility, and regeneration ability for PGB determination.Fil: Gholivand, Mohammad Bagher. Razi University. Faculty of Chemistry; IránFil: Jalalvand, Ali R.. Razi University. Faculty of Chemistry; Irán. Universidad Nacional del Litoral. Cátedra de Química Analítica I. Laboratorio de Desarrollo Analítico y Quimiometría; ArgentinaFil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral. Cátedra de Química Analítica I. Laboratorio de Desarrollo Analítico y Quimiometría; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe; ArgentinaElsevier2014-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/15365Gholivand, Mohammad Bagher; Jalalvand, Ali R.; Goicoechea, Hector Casimiro; Developing a novel computationally designed impedimetric pregabalin biosensor; Elsevier; Electrochimica Acta; 133; 6-2014; 123-1310013-4686enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.electacta.2014.04.017info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S001346861400752Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:41:41Zoai:ri.conicet.gov.ar:11336/15365instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:41:42.105CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Developing a novel computationally designed impedimetric pregabalin biosensor |
title |
Developing a novel computationally designed impedimetric pregabalin biosensor |
spellingShingle |
Developing a novel computationally designed impedimetric pregabalin biosensor Gholivand, Mohammad Bagher Pregabalin Bovine Serum Albumin Graphene Computationally Designed Biosensor |
title_short |
Developing a novel computationally designed impedimetric pregabalin biosensor |
title_full |
Developing a novel computationally designed impedimetric pregabalin biosensor |
title_fullStr |
Developing a novel computationally designed impedimetric pregabalin biosensor |
title_full_unstemmed |
Developing a novel computationally designed impedimetric pregabalin biosensor |
title_sort |
Developing a novel computationally designed impedimetric pregabalin biosensor |
dc.creator.none.fl_str_mv |
Gholivand, Mohammad Bagher Jalalvand, Ali R. Goicoechea, Hector Casimiro |
author |
Gholivand, Mohammad Bagher |
author_facet |
Gholivand, Mohammad Bagher Jalalvand, Ali R. Goicoechea, Hector Casimiro |
author_role |
author |
author2 |
Jalalvand, Ali R. Goicoechea, Hector Casimiro |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Pregabalin Bovine Serum Albumin Graphene Computationally Designed Biosensor |
topic |
Pregabalin Bovine Serum Albumin Graphene Computationally Designed Biosensor |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
A computationally designed impedimetric pregabalin (PGB) biosensor based on immobilization of bovine serum albumin (BSA) onto graphene/glassy carbon electrode (BSA/Gr/GCE) has been developed using initial characterization by computational methods and complementing them by experimental observations. Computational results showed that the BSA hydrophobically binds to Gr which is energetically favorable and leads to the spontaneous formation of the stable nanobiocomposite (BSA/Gr), and also showed that the interaction of PGB with BSA is mainly driven by hydrogen bonding and hydrophobic interactions. The interactions of BSA with Gr and PGB with BSA were also monitored by fluorescence and UVvis spectroscopic techniques, and their results were consistent with the computational results. The electrochemical properties of the fabricated composite electrodes were examined by cyclic voltammetry (CV) scanning electron microscopy (SEM), and electrochemical impedance spectroscopy (EIS) techniques. Besides complementing the computational studies, experimental results showed that the addition of Gr to the surface of the electrode facilitated the electron transfer reactions, and also showed that the presence of BSA inhibits the interfacial electron transfer in some extent due to the non-conductive properties of BSA. The presence of the PGB may form an electroinactive complex with BSA which decelerates the interfacial electron transfer leading to obvious faradaic impedance changes. The faradaic impedance responses were linearly related to PGB concentration between 10.0 nM and 280.0 nM and the limit of detection (LOD) was calculated to be 3.0 nM (3Sb/b). Finally, the proposed biosensor was successfully applied to determination of PGB in human serum samples. The results were satisfactory and comparable to those obtained by applying the reference method based on high performance liquid chromatography (HPLC). The results confirmed that the proposed biosensor has good sensitivity, selectivity, stability, repeatability, reproducibility, and regeneration ability for PGB determination. Fil: Gholivand, Mohammad Bagher. Razi University. Faculty of Chemistry; Irán Fil: Jalalvand, Ali R.. Razi University. Faculty of Chemistry; Irán. Universidad Nacional del Litoral. Cátedra de Química Analítica I. Laboratorio de Desarrollo Analítico y Quimiometría; Argentina Fil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral. Cátedra de Química Analítica I. Laboratorio de Desarrollo Analítico y Quimiometría; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe; Argentina |
description |
A computationally designed impedimetric pregabalin (PGB) biosensor based on immobilization of bovine serum albumin (BSA) onto graphene/glassy carbon electrode (BSA/Gr/GCE) has been developed using initial characterization by computational methods and complementing them by experimental observations. Computational results showed that the BSA hydrophobically binds to Gr which is energetically favorable and leads to the spontaneous formation of the stable nanobiocomposite (BSA/Gr), and also showed that the interaction of PGB with BSA is mainly driven by hydrogen bonding and hydrophobic interactions. The interactions of BSA with Gr and PGB with BSA were also monitored by fluorescence and UVvis spectroscopic techniques, and their results were consistent with the computational results. The electrochemical properties of the fabricated composite electrodes were examined by cyclic voltammetry (CV) scanning electron microscopy (SEM), and electrochemical impedance spectroscopy (EIS) techniques. Besides complementing the computational studies, experimental results showed that the addition of Gr to the surface of the electrode facilitated the electron transfer reactions, and also showed that the presence of BSA inhibits the interfacial electron transfer in some extent due to the non-conductive properties of BSA. The presence of the PGB may form an electroinactive complex with BSA which decelerates the interfacial electron transfer leading to obvious faradaic impedance changes. The faradaic impedance responses were linearly related to PGB concentration between 10.0 nM and 280.0 nM and the limit of detection (LOD) was calculated to be 3.0 nM (3Sb/b). Finally, the proposed biosensor was successfully applied to determination of PGB in human serum samples. The results were satisfactory and comparable to those obtained by applying the reference method based on high performance liquid chromatography (HPLC). The results confirmed that the proposed biosensor has good sensitivity, selectivity, stability, repeatability, reproducibility, and regeneration ability for PGB determination. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-06 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/15365 Gholivand, Mohammad Bagher; Jalalvand, Ali R.; Goicoechea, Hector Casimiro; Developing a novel computationally designed impedimetric pregabalin biosensor; Elsevier; Electrochimica Acta; 133; 6-2014; 123-131 0013-4686 |
url |
http://hdl.handle.net/11336/15365 |
identifier_str_mv |
Gholivand, Mohammad Bagher; Jalalvand, Ali R.; Goicoechea, Hector Casimiro; Developing a novel computationally designed impedimetric pregabalin biosensor; Elsevier; Electrochimica Acta; 133; 6-2014; 123-131 0013-4686 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.electacta.2014.04.017 info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S001346861400752X |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614448350756864 |
score |
13.070432 |