Seminal plasma proteins interacting with sperm surface revert capacitation indicators in frozen-thawed ram sperm

Autores
Ledesma, Alba; Fernández Alegre, Estela; Cano, Adriana; Hozbor, Federico Andrés; Martínez Pastor, Felipe; Cesari, Andreina
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study was conducted to evaluate the effects of interacting seminal plasma proteins (iSPP) obtained by AV or EE on frozen-thawed ram sperm in order to test the hypothesis whether this fraction could be sufficient to emulate the effect of complete seminal plasma (SP). Additionally, we evaluated whether these proteins have a differential effect between spermatozoa from high and low fertility rams and between breeding and non-breeding seasons. We assessed sperm motility, quality parameters (intracellular reactive oxygen species, membrane fluidity, plasma membrane permeability and mitochondrial activity) and capacitation status. The main findings from this work were: i) iSPP had no effect on sperm motility, whereas SP (AV or EE) addition produced the highest values of total motility (74.13 ± 2.99 and 72.27 ± 2.99 for AV and EE, respectively) and progressive motility (64.97 ± 2.64 and 63.73 ± 2.64 for AV and EE, respectively); ii) iSPP had no effect on sperm quality parameters (p > 0.05), but whole SP improved all parameters evaluated. Moreover, SP collected by AV yielded significantly higher viability (44.60 ± 2.87) and sperm with stable plasma membrane (44.56 ± 2.49) comparing with the addition of SP collected by EE (35.80 ± 2.47 and 36.67 ± 1.71, respectively); iii) iSPP and SP collected by EE, but not by AV, reverted molecular signals of capacitation as protein tyrosine phosphorylation caused by freezing temperatures; iv) there were no effects of fertility or season in sperm quality parameters evaluated. This study demonstrated that, although the iSPP have a clear decapacitating effect, including the ability to revert cryo-capacitation indicators, they are not sufficient to emulate the effects of complete SP regarding sperm functional parameters.
Fil: Ledesma, Alba. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernández Alegre, Estela. Universidad de León; España
Fil: Cano, Adriana. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Laboratorio de Biotecnología de la Reproduccion; Argentina
Fil: Hozbor, Federico Andrés. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Laboratorio de Biotecnología de la Reproduccion; Argentina
Fil: Martínez Pastor, Felipe. Universidad de León; España
Fil: Cesari, Andreina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Materia
CAPACITATION
POST-THAW SPERM QUALITY
SEMINAL PLASMA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/62604
Acceder
id CONICETDig_ada1555181d7b017420113d605e85930
oai_identifier_str oai:ri.conicet.gov.ar:11336/62604
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Seminal plasma proteins interacting with sperm surface revert capacitation indicators in frozen-thawed ram spermLedesma, AlbaFernández Alegre, EstelaCano, AdrianaHozbor, Federico AndrésMartínez Pastor, FelipeCesari, AndreinaCAPACITATIONPOST-THAW SPERM QUALITYSEMINAL PLASMAhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4This study was conducted to evaluate the effects of interacting seminal plasma proteins (iSPP) obtained by AV or EE on frozen-thawed ram sperm in order to test the hypothesis whether this fraction could be sufficient to emulate the effect of complete seminal plasma (SP). Additionally, we evaluated whether these proteins have a differential effect between spermatozoa from high and low fertility rams and between breeding and non-breeding seasons. We assessed sperm motility, quality parameters (intracellular reactive oxygen species, membrane fluidity, plasma membrane permeability and mitochondrial activity) and capacitation status. The main findings from this work were: i) iSPP had no effect on sperm motility, whereas SP (AV or EE) addition produced the highest values of total motility (74.13 ± 2.99 and 72.27 ± 2.99 for AV and EE, respectively) and progressive motility (64.97 ± 2.64 and 63.73 ± 2.64 for AV and EE, respectively); ii) iSPP had no effect on sperm quality parameters (p > 0.05), but whole SP improved all parameters evaluated. Moreover, SP collected by AV yielded significantly higher viability (44.60 ± 2.87) and sperm with stable plasma membrane (44.56 ± 2.49) comparing with the addition of SP collected by EE (35.80 ± 2.47 and 36.67 ± 1.71, respectively); iii) iSPP and SP collected by EE, but not by AV, reverted molecular signals of capacitation as protein tyrosine phosphorylation caused by freezing temperatures; iv) there were no effects of fertility or season in sperm quality parameters evaluated. This study demonstrated that, although the iSPP have a clear decapacitating effect, including the ability to revert cryo-capacitation indicators, they are not sufficient to emulate the effects of complete SP regarding sperm functional parameters.Fil: Ledesma, Alba. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fernández Alegre, Estela. Universidad de León; EspañaFil: Cano, Adriana. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Laboratorio de Biotecnología de la Reproduccion; ArgentinaFil: Hozbor, Federico Andrés. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Laboratorio de Biotecnología de la Reproduccion; ArgentinaFil: Martínez Pastor, Felipe. Universidad de León; EspañaFil: Cesari, Andreina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaElsevier Science2016-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/62604Ledesma, Alba; Fernández Alegre, Estela; Cano, Adriana; Hozbor, Federico Andrés; Martínez Pastor, Felipe; et al.; Seminal plasma proteins interacting with sperm surface revert capacitation indicators in frozen-thawed ram sperm; Elsevier Science; Animal Reproduction Science; 173; 10-2016; 35-410378-4320CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.anireprosci.2016.08.007info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0378432016303463info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:55:35Zoai:ri.conicet.gov.ar:11336/62604instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:55:35.881CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Seminal plasma proteins interacting with sperm surface revert capacitation indicators in frozen-thawed ram sperm
title Seminal plasma proteins interacting with sperm surface revert capacitation indicators in frozen-thawed ram sperm
spellingShingle Seminal plasma proteins interacting with sperm surface revert capacitation indicators in frozen-thawed ram sperm
Ledesma, Alba
CAPACITATION
POST-THAW SPERM QUALITY
SEMINAL PLASMA
title_short Seminal plasma proteins interacting with sperm surface revert capacitation indicators in frozen-thawed ram sperm
title_full Seminal plasma proteins interacting with sperm surface revert capacitation indicators in frozen-thawed ram sperm
title_fullStr Seminal plasma proteins interacting with sperm surface revert capacitation indicators in frozen-thawed ram sperm
title_full_unstemmed Seminal plasma proteins interacting with sperm surface revert capacitation indicators in frozen-thawed ram sperm
title_sort Seminal plasma proteins interacting with sperm surface revert capacitation indicators in frozen-thawed ram sperm
dc.creator.none.fl_str_mv Ledesma, Alba
Fernández Alegre, Estela
Cano, Adriana
Hozbor, Federico Andrés
Martínez Pastor, Felipe
Cesari, Andreina
author Ledesma, Alba
author_facet Ledesma, Alba
Fernández Alegre, Estela
Cano, Adriana
Hozbor, Federico Andrés
Martínez Pastor, Felipe
Cesari, Andreina
author_role author
author2 Fernández Alegre, Estela
Cano, Adriana
Hozbor, Federico Andrés
Martínez Pastor, Felipe
Cesari, Andreina
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv CAPACITATION
POST-THAW SPERM QUALITY
SEMINAL PLASMA
topic CAPACITATION
POST-THAW SPERM QUALITY
SEMINAL PLASMA
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv This study was conducted to evaluate the effects of interacting seminal plasma proteins (iSPP) obtained by AV or EE on frozen-thawed ram sperm in order to test the hypothesis whether this fraction could be sufficient to emulate the effect of complete seminal plasma (SP). Additionally, we evaluated whether these proteins have a differential effect between spermatozoa from high and low fertility rams and between breeding and non-breeding seasons. We assessed sperm motility, quality parameters (intracellular reactive oxygen species, membrane fluidity, plasma membrane permeability and mitochondrial activity) and capacitation status. The main findings from this work were: i) iSPP had no effect on sperm motility, whereas SP (AV or EE) addition produced the highest values of total motility (74.13 ± 2.99 and 72.27 ± 2.99 for AV and EE, respectively) and progressive motility (64.97 ± 2.64 and 63.73 ± 2.64 for AV and EE, respectively); ii) iSPP had no effect on sperm quality parameters (p > 0.05), but whole SP improved all parameters evaluated. Moreover, SP collected by AV yielded significantly higher viability (44.60 ± 2.87) and sperm with stable plasma membrane (44.56 ± 2.49) comparing with the addition of SP collected by EE (35.80 ± 2.47 and 36.67 ± 1.71, respectively); iii) iSPP and SP collected by EE, but not by AV, reverted molecular signals of capacitation as protein tyrosine phosphorylation caused by freezing temperatures; iv) there were no effects of fertility or season in sperm quality parameters evaluated. This study demonstrated that, although the iSPP have a clear decapacitating effect, including the ability to revert cryo-capacitation indicators, they are not sufficient to emulate the effects of complete SP regarding sperm functional parameters.
Fil: Ledesma, Alba. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernández Alegre, Estela. Universidad de León; España
Fil: Cano, Adriana. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Laboratorio de Biotecnología de la Reproduccion; Argentina
Fil: Hozbor, Federico Andrés. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Laboratorio de Biotecnología de la Reproduccion; Argentina
Fil: Martínez Pastor, Felipe. Universidad de León; España
Fil: Cesari, Andreina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
description This study was conducted to evaluate the effects of interacting seminal plasma proteins (iSPP) obtained by AV or EE on frozen-thawed ram sperm in order to test the hypothesis whether this fraction could be sufficient to emulate the effect of complete seminal plasma (SP). Additionally, we evaluated whether these proteins have a differential effect between spermatozoa from high and low fertility rams and between breeding and non-breeding seasons. We assessed sperm motility, quality parameters (intracellular reactive oxygen species, membrane fluidity, plasma membrane permeability and mitochondrial activity) and capacitation status. The main findings from this work were: i) iSPP had no effect on sperm motility, whereas SP (AV or EE) addition produced the highest values of total motility (74.13 ± 2.99 and 72.27 ± 2.99 for AV and EE, respectively) and progressive motility (64.97 ± 2.64 and 63.73 ± 2.64 for AV and EE, respectively); ii) iSPP had no effect on sperm quality parameters (p > 0.05), but whole SP improved all parameters evaluated. Moreover, SP collected by AV yielded significantly higher viability (44.60 ± 2.87) and sperm with stable plasma membrane (44.56 ± 2.49) comparing with the addition of SP collected by EE (35.80 ± 2.47 and 36.67 ± 1.71, respectively); iii) iSPP and SP collected by EE, but not by AV, reverted molecular signals of capacitation as protein tyrosine phosphorylation caused by freezing temperatures; iv) there were no effects of fertility or season in sperm quality parameters evaluated. This study demonstrated that, although the iSPP have a clear decapacitating effect, including the ability to revert cryo-capacitation indicators, they are not sufficient to emulate the effects of complete SP regarding sperm functional parameters.
publishDate 2016
dc.date.none.fl_str_mv 2016-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/62604
Ledesma, Alba; Fernández Alegre, Estela; Cano, Adriana; Hozbor, Federico Andrés; Martínez Pastor, Felipe; et al.; Seminal plasma proteins interacting with sperm surface revert capacitation indicators in frozen-thawed ram sperm; Elsevier Science; Animal Reproduction Science; 173; 10-2016; 35-41
0378-4320
CONICET Digital
CONICET
url http://hdl.handle.net/11336/62604
identifier_str_mv Ledesma, Alba; Fernández Alegre, Estela; Cano, Adriana; Hozbor, Federico Andrés; Martínez Pastor, Felipe; et al.; Seminal plasma proteins interacting with sperm surface revert capacitation indicators in frozen-thawed ram sperm; Elsevier Science; Animal Reproduction Science; 173; 10-2016; 35-41
0378-4320
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.anireprosci.2016.08.007
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0378432016303463
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269354524147712
score 13.13397

Publicaciones similares