Sequestration of 9-Hydroxystearic Acid in FAHFA (Fatty Acid Esters of Hydroxy Fatty Acids) as a Protective Mechanism for Colon Carcinoma Cells to Avoid Apoptotic Cell Death

Autores
Rodríguez, Juan Pablo; Guijas, Carlos; Astudillo, Alma M.; Rubio, Julio M.; Balboa, María A.; Balsinde Rodríguez, Jesús
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Hydroxy fatty acids are known to cause cell cycle arrest and apoptosis. The best studied of them, 9-hydroxystearic acid (9-HSA), induces apoptosis in cell lines by acting through mechanisms involving different targets. Using mass spectrometry-based lipidomic approaches, we show in this study that 9-HSA levels in human colorectal tumors are diminished when compared with normal adjacent tissue. Since this decrease could be compatible with an escape mechanism of tumors from 9-HSA-induced apoptosis, we investigated different features of the utilization of this hydroxyfatty acid in colon. We show that in colorectal tumors and related cell lines such as HT-29 and HCT-116, 9-HSA is the only hydroxyfatty acid constituent of branched fatty acid esters of hydroxyfatty acids (FAHFA), a novel family of lipids with anti-inflammatory properties. Importantly, FAHFA levels in tumors are elevated compared with normal tissue and, unlike 9-HSA, they do not induce apoptosis of colorectal cell lines over a wide range of concentrations. Further, the addition of 9-HSA to colon cancer cell lines augments the synthesis of different FAHFA before the cells commit to apoptosis, suggesting that FAHFA formation may function as a buffer system that sequesters the hydroxyacid into an inactive form, thereby restricting apoptosis.
Fil: Rodríguez, Juan Pablo. Consejo Superior de Investigaciones Científicas; España. Universidad de Valladolid; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
Fil: Guijas, Carlos. Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas; España. Universidad de Valladolid; España
Fil: Astudillo, Alma M.. Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas; España. Universidad de Valladolid; España
Fil: Rubio, Julio M.. Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas; España. Universidad de Valladolid; España
Fil: Balboa, María A.. Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas; España. Universidad de Valladolid; España
Fil: Balsinde Rodríguez, Jesús. Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas; España. Universidad de Valladolid; España
Materia
APOPTOSIS
COLORECTAL CANCER
FATTY ACID ESTERS OF HYDROXY FATTY ACIDS
HYDROXYSTEARIC ACID
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/108583
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Sequestration of 9-Hydroxystearic Acid in FAHFA (Fatty Acid Esters of Hydroxy Fatty Acids) as a Protective Mechanism for Colon Carcinoma Cells to Avoid Apoptotic Cell DeathRodríguez, Juan PabloGuijas, CarlosAstudillo, Alma M.Rubio, Julio M.Balboa, María A.Balsinde Rodríguez, JesúsAPOPTOSISCOLORECTAL CANCERFATTY ACID ESTERS OF HYDROXY FATTY ACIDSHYDROXYSTEARIC ACIDhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Hydroxy fatty acids are known to cause cell cycle arrest and apoptosis. The best studied of them, 9-hydroxystearic acid (9-HSA), induces apoptosis in cell lines by acting through mechanisms involving different targets. Using mass spectrometry-based lipidomic approaches, we show in this study that 9-HSA levels in human colorectal tumors are diminished when compared with normal adjacent tissue. Since this decrease could be compatible with an escape mechanism of tumors from 9-HSA-induced apoptosis, we investigated different features of the utilization of this hydroxyfatty acid in colon. We show that in colorectal tumors and related cell lines such as HT-29 and HCT-116, 9-HSA is the only hydroxyfatty acid constituent of branched fatty acid esters of hydroxyfatty acids (FAHFA), a novel family of lipids with anti-inflammatory properties. Importantly, FAHFA levels in tumors are elevated compared with normal tissue and, unlike 9-HSA, they do not induce apoptosis of colorectal cell lines over a wide range of concentrations. Further, the addition of 9-HSA to colon cancer cell lines augments the synthesis of different FAHFA before the cells commit to apoptosis, suggesting that FAHFA formation may function as a buffer system that sequesters the hydroxyacid into an inactive form, thereby restricting apoptosis.Fil: Rodríguez, Juan Pablo. Consejo Superior de Investigaciones Científicas; España. Universidad de Valladolid; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; ArgentinaFil: Guijas, Carlos. Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas; España. Universidad de Valladolid; EspañaFil: Astudillo, Alma M.. Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas; España. Universidad de Valladolid; EspañaFil: Rubio, Julio M.. Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas; España. Universidad de Valladolid; EspañaFil: Balboa, María A.. Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas; España. Universidad de Valladolid; EspañaFil: Balsinde Rodríguez, Jesús. Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas; España. Universidad de Valladolid; EspañaMDPI2019-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/108583Rodríguez, Juan Pablo; Guijas, Carlos; Astudillo, Alma M.; Rubio, Julio M.; Balboa, María A.; et al.; Sequestration of 9-Hydroxystearic Acid in FAHFA (Fatty Acid Esters of Hydroxy Fatty Acids) as a Protective Mechanism for Colon Carcinoma Cells to Avoid Apoptotic Cell Death; MDPI; Cancers; 11; 4; 4-20192072-6694CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2072-6694/11/4/524info:eu-repo/semantics/altIdentifier/doi/10.3390/cancers11040524info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:55:49Zoai:ri.conicet.gov.ar:11336/108583instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:55:49.567CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Sequestration of 9-Hydroxystearic Acid in FAHFA (Fatty Acid Esters of Hydroxy Fatty Acids) as a Protective Mechanism for Colon Carcinoma Cells to Avoid Apoptotic Cell Death
title Sequestration of 9-Hydroxystearic Acid in FAHFA (Fatty Acid Esters of Hydroxy Fatty Acids) as a Protective Mechanism for Colon Carcinoma Cells to Avoid Apoptotic Cell Death
spellingShingle Sequestration of 9-Hydroxystearic Acid in FAHFA (Fatty Acid Esters of Hydroxy Fatty Acids) as a Protective Mechanism for Colon Carcinoma Cells to Avoid Apoptotic Cell Death
Rodríguez, Juan Pablo
APOPTOSIS
COLORECTAL CANCER
FATTY ACID ESTERS OF HYDROXY FATTY ACIDS
HYDROXYSTEARIC ACID
title_short Sequestration of 9-Hydroxystearic Acid in FAHFA (Fatty Acid Esters of Hydroxy Fatty Acids) as a Protective Mechanism for Colon Carcinoma Cells to Avoid Apoptotic Cell Death
title_full Sequestration of 9-Hydroxystearic Acid in FAHFA (Fatty Acid Esters of Hydroxy Fatty Acids) as a Protective Mechanism for Colon Carcinoma Cells to Avoid Apoptotic Cell Death
title_fullStr Sequestration of 9-Hydroxystearic Acid in FAHFA (Fatty Acid Esters of Hydroxy Fatty Acids) as a Protective Mechanism for Colon Carcinoma Cells to Avoid Apoptotic Cell Death
title_full_unstemmed Sequestration of 9-Hydroxystearic Acid in FAHFA (Fatty Acid Esters of Hydroxy Fatty Acids) as a Protective Mechanism for Colon Carcinoma Cells to Avoid Apoptotic Cell Death
title_sort Sequestration of 9-Hydroxystearic Acid in FAHFA (Fatty Acid Esters of Hydroxy Fatty Acids) as a Protective Mechanism for Colon Carcinoma Cells to Avoid Apoptotic Cell Death
dc.creator.none.fl_str_mv Rodríguez, Juan Pablo
Guijas, Carlos
Astudillo, Alma M.
Rubio, Julio M.
Balboa, María A.
Balsinde Rodríguez, Jesús
author Rodríguez, Juan Pablo
author_facet Rodríguez, Juan Pablo
Guijas, Carlos
Astudillo, Alma M.
Rubio, Julio M.
Balboa, María A.
Balsinde Rodríguez, Jesús
author_role author
author2 Guijas, Carlos
Astudillo, Alma M.
Rubio, Julio M.
Balboa, María A.
Balsinde Rodríguez, Jesús
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv APOPTOSIS
COLORECTAL CANCER
FATTY ACID ESTERS OF HYDROXY FATTY ACIDS
HYDROXYSTEARIC ACID
topic APOPTOSIS
COLORECTAL CANCER
FATTY ACID ESTERS OF HYDROXY FATTY ACIDS
HYDROXYSTEARIC ACID
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Hydroxy fatty acids are known to cause cell cycle arrest and apoptosis. The best studied of them, 9-hydroxystearic acid (9-HSA), induces apoptosis in cell lines by acting through mechanisms involving different targets. Using mass spectrometry-based lipidomic approaches, we show in this study that 9-HSA levels in human colorectal tumors are diminished when compared with normal adjacent tissue. Since this decrease could be compatible with an escape mechanism of tumors from 9-HSA-induced apoptosis, we investigated different features of the utilization of this hydroxyfatty acid in colon. We show that in colorectal tumors and related cell lines such as HT-29 and HCT-116, 9-HSA is the only hydroxyfatty acid constituent of branched fatty acid esters of hydroxyfatty acids (FAHFA), a novel family of lipids with anti-inflammatory properties. Importantly, FAHFA levels in tumors are elevated compared with normal tissue and, unlike 9-HSA, they do not induce apoptosis of colorectal cell lines over a wide range of concentrations. Further, the addition of 9-HSA to colon cancer cell lines augments the synthesis of different FAHFA before the cells commit to apoptosis, suggesting that FAHFA formation may function as a buffer system that sequesters the hydroxyacid into an inactive form, thereby restricting apoptosis.
Fil: Rodríguez, Juan Pablo. Consejo Superior de Investigaciones Científicas; España. Universidad de Valladolid; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
Fil: Guijas, Carlos. Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas; España. Universidad de Valladolid; España
Fil: Astudillo, Alma M.. Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas; España. Universidad de Valladolid; España
Fil: Rubio, Julio M.. Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas; España. Universidad de Valladolid; España
Fil: Balboa, María A.. Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas; España. Universidad de Valladolid; España
Fil: Balsinde Rodríguez, Jesús. Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas; España. Universidad de Valladolid; España
description Hydroxy fatty acids are known to cause cell cycle arrest and apoptosis. The best studied of them, 9-hydroxystearic acid (9-HSA), induces apoptosis in cell lines by acting through mechanisms involving different targets. Using mass spectrometry-based lipidomic approaches, we show in this study that 9-HSA levels in human colorectal tumors are diminished when compared with normal adjacent tissue. Since this decrease could be compatible with an escape mechanism of tumors from 9-HSA-induced apoptosis, we investigated different features of the utilization of this hydroxyfatty acid in colon. We show that in colorectal tumors and related cell lines such as HT-29 and HCT-116, 9-HSA is the only hydroxyfatty acid constituent of branched fatty acid esters of hydroxyfatty acids (FAHFA), a novel family of lipids with anti-inflammatory properties. Importantly, FAHFA levels in tumors are elevated compared with normal tissue and, unlike 9-HSA, they do not induce apoptosis of colorectal cell lines over a wide range of concentrations. Further, the addition of 9-HSA to colon cancer cell lines augments the synthesis of different FAHFA before the cells commit to apoptosis, suggesting that FAHFA formation may function as a buffer system that sequesters the hydroxyacid into an inactive form, thereby restricting apoptosis.
publishDate 2019
dc.date.none.fl_str_mv 2019-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/108583
Rodríguez, Juan Pablo; Guijas, Carlos; Astudillo, Alma M.; Rubio, Julio M.; Balboa, María A.; et al.; Sequestration of 9-Hydroxystearic Acid in FAHFA (Fatty Acid Esters of Hydroxy Fatty Acids) as a Protective Mechanism for Colon Carcinoma Cells to Avoid Apoptotic Cell Death; MDPI; Cancers; 11; 4; 4-2019
2072-6694
CONICET Digital
CONICET
url http://hdl.handle.net/11336/108583
identifier_str_mv Rodríguez, Juan Pablo; Guijas, Carlos; Astudillo, Alma M.; Rubio, Julio M.; Balboa, María A.; et al.; Sequestration of 9-Hydroxystearic Acid in FAHFA (Fatty Acid Esters of Hydroxy Fatty Acids) as a Protective Mechanism for Colon Carcinoma Cells to Avoid Apoptotic Cell Death; MDPI; Cancers; 11; 4; 4-2019
2072-6694
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2072-6694/11/4/524
info:eu-repo/semantics/altIdentifier/doi/10.3390/cancers11040524
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.976206

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