Ultra sensitive microfluidic immunosensor for determination of clenbuterol in bovine hair samples using electrodeposited gold nanoparticles and magnetic microparticles as bio-affin...

Autores
Regiart, Daniel Matias Gaston; Fernández Baldo, Martín Alejandro; Spotorno, Viviana G.; Bertolino, Franco Adrián; Raba, Julio
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this article, we report the first integrated microfluidic immunosensor coupled to a screen-printed carbon electrode (SPCE) applied to determination of clenbuterol (CLB) in bovine hair samples. CLB is a member of the β2-agonist drugs which is used in animal production and is banned in Argentine and the European Union. It represents a potential risk and has to be carefully monitored to avoid the illegal use of high amounts of this compound that could result in human food poisoning. In order to perform the CLB detection, the SPCE was modified by gold nanoparticles (AuNPs) electrodeposition. Quantitative determination of CLB was carried out using a competitive indirect immunoassay, method based on the use of anti-CLB antibodies immobilized on magnetic micro particles. The CLB present in bovine hair samples competes immunologically with alkaline phosphatase (AP) enzyme-labeled CLB conjugate for the anti-CLB specific antibodies. Later, p-aminophenyl phosphate was converted to p-aminophenol by AP, and the electroactive product was quantified on AuNPs/SPCE at +0.1 V. The limit of detection for electrochemical method was 0.008 ng mL−1 and the intra- and inter-assay coefficients of variation were below 6%. This being a veterinary control tool very useful for rapid, sensitive and selective detection of CLB in an “in vitro” technique.
Fil: Regiart, Daniel Matias Gaston. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Spotorno, Viviana G.. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Recursos Naturales. Instituto de Recursos Biológicos; Argentina
Fil: Bertolino, Franco Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Materia
Microfluidic Immunosensor
Clenbuterol
Bovine Hair Samples
Gold Nanoparticles
Magnetic Micro Particles
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/5756
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/5756
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Ultra sensitive microfluidic immunosensor for determination of clenbuterol in bovine hair samples using electrodeposited gold nanoparticles and magnetic microparticles as bio-affinity platformRegiart, Daniel Matias GastonFernández Baldo, Martín AlejandroSpotorno, Viviana G.Bertolino, Franco AdriánRaba, JulioMicrofluidic ImmunosensorClenbuterolBovine Hair SamplesGold NanoparticlesMagnetic Micro Particleshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1In this article, we report the first integrated microfluidic immunosensor coupled to a screen-printed carbon electrode (SPCE) applied to determination of clenbuterol (CLB) in bovine hair samples. CLB is a member of the β2-agonist drugs which is used in animal production and is banned in Argentine and the European Union. It represents a potential risk and has to be carefully monitored to avoid the illegal use of high amounts of this compound that could result in human food poisoning. In order to perform the CLB detection, the SPCE was modified by gold nanoparticles (AuNPs) electrodeposition. Quantitative determination of CLB was carried out using a competitive indirect immunoassay, method based on the use of anti-CLB antibodies immobilized on magnetic micro particles. The CLB present in bovine hair samples competes immunologically with alkaline phosphatase (AP) enzyme-labeled CLB conjugate for the anti-CLB specific antibodies. Later, p-aminophenyl phosphate was converted to p-aminophenol by AP, and the electroactive product was quantified on AuNPs/SPCE at +0.1 V. The limit of detection for electrochemical method was 0.008 ng mL−1 and the intra- and inter-assay coefficients of variation were below 6%. This being a veterinary control tool very useful for rapid, sensitive and selective detection of CLB in an “in vitro” technique.Fil: Regiart, Daniel Matias Gaston. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; ArgentinaFil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; ArgentinaFil: Spotorno, Viviana G.. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Recursos Naturales. Instituto de Recursos Biológicos; ArgentinaFil: Bertolino, Franco Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; ArgentinaFil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; ArgentinaElsevier2013-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/5756Regiart, Daniel Matias Gaston; Fernández Baldo, Martín Alejandro; Spotorno, Viviana G.; Bertolino, Franco Adrián; Raba, Julio; Ultra sensitive microfluidic immunosensor for determination of clenbuterol in bovine hair samples using electrodeposited gold nanoparticles and magnetic microparticles as bio-affinity platform; Elsevier; Biosensors & Bioelectronics; 41; 3-2013; 211-2170956-5663enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0956566312005441info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bios.2012.08.020info:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:37Zoai:ri.conicet.gov.ar:11336/5756instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:37.297CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Ultra sensitive microfluidic immunosensor for determination of clenbuterol in bovine hair samples using electrodeposited gold nanoparticles and magnetic microparticles as bio-affinity platform
title Ultra sensitive microfluidic immunosensor for determination of clenbuterol in bovine hair samples using electrodeposited gold nanoparticles and magnetic microparticles as bio-affinity platform
spellingShingle Ultra sensitive microfluidic immunosensor for determination of clenbuterol in bovine hair samples using electrodeposited gold nanoparticles and magnetic microparticles as bio-affinity platform
Regiart, Daniel Matias Gaston
Microfluidic Immunosensor
Clenbuterol
Bovine Hair Samples
Gold Nanoparticles
Magnetic Micro Particles
title_short Ultra sensitive microfluidic immunosensor for determination of clenbuterol in bovine hair samples using electrodeposited gold nanoparticles and magnetic microparticles as bio-affinity platform
title_full Ultra sensitive microfluidic immunosensor for determination of clenbuterol in bovine hair samples using electrodeposited gold nanoparticles and magnetic microparticles as bio-affinity platform
title_fullStr Ultra sensitive microfluidic immunosensor for determination of clenbuterol in bovine hair samples using electrodeposited gold nanoparticles and magnetic microparticles as bio-affinity platform
title_full_unstemmed Ultra sensitive microfluidic immunosensor for determination of clenbuterol in bovine hair samples using electrodeposited gold nanoparticles and magnetic microparticles as bio-affinity platform
title_sort Ultra sensitive microfluidic immunosensor for determination of clenbuterol in bovine hair samples using electrodeposited gold nanoparticles and magnetic microparticles as bio-affinity platform
dc.creator.none.fl_str_mv Regiart, Daniel Matias Gaston
Fernández Baldo, Martín Alejandro
Spotorno, Viviana G.
Bertolino, Franco Adrián
Raba, Julio
author Regiart, Daniel Matias Gaston
author_facet Regiart, Daniel Matias Gaston
Fernández Baldo, Martín Alejandro
Spotorno, Viviana G.
Bertolino, Franco Adrián
Raba, Julio
author_role author
author2 Fernández Baldo, Martín Alejandro
Spotorno, Viviana G.
Bertolino, Franco Adrián
Raba, Julio
author2_role author
author
author
author
dc.subject.none.fl_str_mv Microfluidic Immunosensor
Clenbuterol
Bovine Hair Samples
Gold Nanoparticles
Magnetic Micro Particles
topic Microfluidic Immunosensor
Clenbuterol
Bovine Hair Samples
Gold Nanoparticles
Magnetic Micro Particles
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In this article, we report the first integrated microfluidic immunosensor coupled to a screen-printed carbon electrode (SPCE) applied to determination of clenbuterol (CLB) in bovine hair samples. CLB is a member of the β2-agonist drugs which is used in animal production and is banned in Argentine and the European Union. It represents a potential risk and has to be carefully monitored to avoid the illegal use of high amounts of this compound that could result in human food poisoning. In order to perform the CLB detection, the SPCE was modified by gold nanoparticles (AuNPs) electrodeposition. Quantitative determination of CLB was carried out using a competitive indirect immunoassay, method based on the use of anti-CLB antibodies immobilized on magnetic micro particles. The CLB present in bovine hair samples competes immunologically with alkaline phosphatase (AP) enzyme-labeled CLB conjugate for the anti-CLB specific antibodies. Later, p-aminophenyl phosphate was converted to p-aminophenol by AP, and the electroactive product was quantified on AuNPs/SPCE at +0.1 V. The limit of detection for electrochemical method was 0.008 ng mL−1 and the intra- and inter-assay coefficients of variation were below 6%. This being a veterinary control tool very useful for rapid, sensitive and selective detection of CLB in an “in vitro” technique.
Fil: Regiart, Daniel Matias Gaston. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Spotorno, Viviana G.. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Recursos Naturales. Instituto de Recursos Biológicos; Argentina
Fil: Bertolino, Franco Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
description In this article, we report the first integrated microfluidic immunosensor coupled to a screen-printed carbon electrode (SPCE) applied to determination of clenbuterol (CLB) in bovine hair samples. CLB is a member of the β2-agonist drugs which is used in animal production and is banned in Argentine and the European Union. It represents a potential risk and has to be carefully monitored to avoid the illegal use of high amounts of this compound that could result in human food poisoning. In order to perform the CLB detection, the SPCE was modified by gold nanoparticles (AuNPs) electrodeposition. Quantitative determination of CLB was carried out using a competitive indirect immunoassay, method based on the use of anti-CLB antibodies immobilized on magnetic micro particles. The CLB present in bovine hair samples competes immunologically with alkaline phosphatase (AP) enzyme-labeled CLB conjugate for the anti-CLB specific antibodies. Later, p-aminophenyl phosphate was converted to p-aminophenol by AP, and the electroactive product was quantified on AuNPs/SPCE at +0.1 V. The limit of detection for electrochemical method was 0.008 ng mL−1 and the intra- and inter-assay coefficients of variation were below 6%. This being a veterinary control tool very useful for rapid, sensitive and selective detection of CLB in an “in vitro” technique.
publishDate 2013
dc.date.none.fl_str_mv 2013-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/5756
Regiart, Daniel Matias Gaston; Fernández Baldo, Martín Alejandro; Spotorno, Viviana G.; Bertolino, Franco Adrián; Raba, Julio; Ultra sensitive microfluidic immunosensor for determination of clenbuterol in bovine hair samples using electrodeposited gold nanoparticles and magnetic microparticles as bio-affinity platform; Elsevier; Biosensors & Bioelectronics; 41; 3-2013; 211-217
0956-5663
url http://hdl.handle.net/11336/5756
identifier_str_mv Regiart, Daniel Matias Gaston; Fernández Baldo, Martín Alejandro; Spotorno, Viviana G.; Bertolino, Franco Adrián; Raba, Julio; Ultra sensitive microfluidic immunosensor for determination of clenbuterol in bovine hair samples using electrodeposited gold nanoparticles and magnetic microparticles as bio-affinity platform; Elsevier; Biosensors & Bioelectronics; 41; 3-2013; 211-217
0956-5663
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0956566312005441
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bios.2012.08.020
info:eu-repo/semantics/altIdentifier/doi/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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