Activity of free peroxidases, hematin, magnetite-supported peroxidases and magnetite-supported hematin in the aniline elimination from water-UV-vis analysis

Autores
Saidman, Silvana Beatriz; Rueda, Elsa Haydee; Ferreira, María Luján
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This manuscript presents the UV-vis study of the aniline elimination by polymerization using peroxidases from different sources and hematin, free and supported. The use of magnetite-supported hematin has potential in the implementation of aniline elimination by polymerization in acidic media at pH 4. The temperature must be selected between 45 and 65°C. Crude preparations of Pleurotus sajor caju (PSC) are an alternative to purified Horseradish peroxidase (HRP) as the catalyst of the reaction. Pernigraniline is the main species found in the reaction media after aniline elimination procedure. Supported oxidoreductases are active biocatalysts and conversion of aniline in the 30-90% range can be obtained, depending on the catalyst. The UV-vis spectra show bands corresponding to base and protoned (blue and purple-deep red) pernigraniline, a contribution of quinoid species at 480 nm and a minor contribution of base blue emeraldine and another oxidation intermediate states between pernigraniline and emeraldine. At selected conditions, the recovered solid showed greenish/reddish coloration. The goal is the precipitation of the aniline as polyaniline. Supported biocatalysts were less active than free enzymes.
Fil: Saidman, Silvana Beatriz. Universidad Nacional del Sur; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rueda, Elsa Haydee. Universidad Nacional del Sur; Argentina
Fil: Ferreira, María Luján. Universidad Nacional del Sur; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
Materia
Enzymatic
Hematin
Magnetite
Peroxidase
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/37701
Acceder
id CONICETDig_acceb85468760b6960d3b76f1ae33a88
oai_identifier_str oai:ri.conicet.gov.ar:11336/37701
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Activity of free peroxidases, hematin, magnetite-supported peroxidases and magnetite-supported hematin in the aniline elimination from water-UV-vis analysisSaidman, Silvana BeatrizRueda, Elsa HaydeeFerreira, María LujánEnzymaticHematinMagnetitePeroxidasehttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1This manuscript presents the UV-vis study of the aniline elimination by polymerization using peroxidases from different sources and hematin, free and supported. The use of magnetite-supported hematin has potential in the implementation of aniline elimination by polymerization in acidic media at pH 4. The temperature must be selected between 45 and 65°C. Crude preparations of Pleurotus sajor caju (PSC) are an alternative to purified Horseradish peroxidase (HRP) as the catalyst of the reaction. Pernigraniline is the main species found in the reaction media after aniline elimination procedure. Supported oxidoreductases are active biocatalysts and conversion of aniline in the 30-90% range can be obtained, depending on the catalyst. The UV-vis spectra show bands corresponding to base and protoned (blue and purple-deep red) pernigraniline, a contribution of quinoid species at 480 nm and a minor contribution of base blue emeraldine and another oxidation intermediate states between pernigraniline and emeraldine. At selected conditions, the recovered solid showed greenish/reddish coloration. The goal is the precipitation of the aniline as polyaniline. Supported biocatalysts were less active than free enzymes.Fil: Saidman, Silvana Beatriz. Universidad Nacional del Sur; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rueda, Elsa Haydee. Universidad Nacional del Sur; ArgentinaFil: Ferreira, María Luján. Universidad Nacional del Sur; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; ArgentinaElsevier Science Sa2006-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/37701Saidman, Silvana Beatriz; Rueda, Elsa Haydee; Ferreira, María Luján; Activity of free peroxidases, hematin, magnetite-supported peroxidases and magnetite-supported hematin in the aniline elimination from water-UV-vis analysis; Elsevier Science Sa; Biochemical Engineering Journal; 28; 2; 2-2006; 177-1861369-703XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.bej.2005.11.001info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1369703X05003505info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:58:03Zoai:ri.conicet.gov.ar:11336/37701instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:58:03.804CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Activity of free peroxidases, hematin, magnetite-supported peroxidases and magnetite-supported hematin in the aniline elimination from water-UV-vis analysis
title Activity of free peroxidases, hematin, magnetite-supported peroxidases and magnetite-supported hematin in the aniline elimination from water-UV-vis analysis
spellingShingle Activity of free peroxidases, hematin, magnetite-supported peroxidases and magnetite-supported hematin in the aniline elimination from water-UV-vis analysis
Saidman, Silvana Beatriz
Enzymatic
Hematin
Magnetite
Peroxidase
title_short Activity of free peroxidases, hematin, magnetite-supported peroxidases and magnetite-supported hematin in the aniline elimination from water-UV-vis analysis
title_full Activity of free peroxidases, hematin, magnetite-supported peroxidases and magnetite-supported hematin in the aniline elimination from water-UV-vis analysis
title_fullStr Activity of free peroxidases, hematin, magnetite-supported peroxidases and magnetite-supported hematin in the aniline elimination from water-UV-vis analysis
title_full_unstemmed Activity of free peroxidases, hematin, magnetite-supported peroxidases and magnetite-supported hematin in the aniline elimination from water-UV-vis analysis
title_sort Activity of free peroxidases, hematin, magnetite-supported peroxidases and magnetite-supported hematin in the aniline elimination from water-UV-vis analysis
dc.creator.none.fl_str_mv Saidman, Silvana Beatriz
Rueda, Elsa Haydee
Ferreira, María Luján
author Saidman, Silvana Beatriz
author_facet Saidman, Silvana Beatriz
Rueda, Elsa Haydee
Ferreira, María Luján
author_role author
author2 Rueda, Elsa Haydee
Ferreira, María Luján
author2_role author
author
dc.subject.none.fl_str_mv Enzymatic
Hematin
Magnetite
Peroxidase
topic Enzymatic
Hematin
Magnetite
Peroxidase
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This manuscript presents the UV-vis study of the aniline elimination by polymerization using peroxidases from different sources and hematin, free and supported. The use of magnetite-supported hematin has potential in the implementation of aniline elimination by polymerization in acidic media at pH 4. The temperature must be selected between 45 and 65°C. Crude preparations of Pleurotus sajor caju (PSC) are an alternative to purified Horseradish peroxidase (HRP) as the catalyst of the reaction. Pernigraniline is the main species found in the reaction media after aniline elimination procedure. Supported oxidoreductases are active biocatalysts and conversion of aniline in the 30-90% range can be obtained, depending on the catalyst. The UV-vis spectra show bands corresponding to base and protoned (blue and purple-deep red) pernigraniline, a contribution of quinoid species at 480 nm and a minor contribution of base blue emeraldine and another oxidation intermediate states between pernigraniline and emeraldine. At selected conditions, the recovered solid showed greenish/reddish coloration. The goal is the precipitation of the aniline as polyaniline. Supported biocatalysts were less active than free enzymes.
Fil: Saidman, Silvana Beatriz. Universidad Nacional del Sur; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rueda, Elsa Haydee. Universidad Nacional del Sur; Argentina
Fil: Ferreira, María Luján. Universidad Nacional del Sur; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
description This manuscript presents the UV-vis study of the aniline elimination by polymerization using peroxidases from different sources and hematin, free and supported. The use of magnetite-supported hematin has potential in the implementation of aniline elimination by polymerization in acidic media at pH 4. The temperature must be selected between 45 and 65°C. Crude preparations of Pleurotus sajor caju (PSC) are an alternative to purified Horseradish peroxidase (HRP) as the catalyst of the reaction. Pernigraniline is the main species found in the reaction media after aniline elimination procedure. Supported oxidoreductases are active biocatalysts and conversion of aniline in the 30-90% range can be obtained, depending on the catalyst. The UV-vis spectra show bands corresponding to base and protoned (blue and purple-deep red) pernigraniline, a contribution of quinoid species at 480 nm and a minor contribution of base blue emeraldine and another oxidation intermediate states between pernigraniline and emeraldine. At selected conditions, the recovered solid showed greenish/reddish coloration. The goal is the precipitation of the aniline as polyaniline. Supported biocatalysts were less active than free enzymes.
publishDate 2006
dc.date.none.fl_str_mv 2006-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/37701
Saidman, Silvana Beatriz; Rueda, Elsa Haydee; Ferreira, María Luján; Activity of free peroxidases, hematin, magnetite-supported peroxidases and magnetite-supported hematin in the aniline elimination from water-UV-vis analysis; Elsevier Science Sa; Biochemical Engineering Journal; 28; 2; 2-2006; 177-186
1369-703X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/37701
identifier_str_mv Saidman, Silvana Beatriz; Rueda, Elsa Haydee; Ferreira, María Luján; Activity of free peroxidases, hematin, magnetite-supported peroxidases and magnetite-supported hematin in the aniline elimination from water-UV-vis analysis; Elsevier Science Sa; Biochemical Engineering Journal; 28; 2; 2-2006; 177-186
1369-703X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bej.2005.11.001
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1369703X05003505
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Sa
publisher.none.fl_str_mv Elsevier Science Sa
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613732294983680
score 13.070432

Publicaciones similares