Modified chitosan as an economic support for hematin: Application in the decolorization of anthraquinone and azo dyes

Autores
Córdoba, Agostina; Magario, Ivana; Ferreira, María Luján
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Azo and anthraquinone dyes have been successfully decolorizedusing enzymatic and biomimetic homogeneoussystems.1,2 Hematin, a horseradish peroxidase (HRP) biomimetic, immobilized on chitosan via glutaraldehyde couplingwas ableto decolorize Alizarin Red S and Orange II solutions. A Doehlert experimental design and response surface analysis was appliedto determine conditions for optimalmass of catalyst and catalytic efficiency of the immobilization procedure.RESULTS: The catalysts with supported hematin showed 33% activity (relative to homogeneous hematin as 100%) in decolorizationreactions. After six reuses the catalytic activity was maintained at 60% of the initial one. Hematin anchoring to chitosanwithout alteration of the iron-porphyrin ring was confirmed by ICP, FTIR and UV/visible spectrophotometric methods. Inaddition, the effect on hematin activity in the decolorization of aminopropyltriethoxysilane (APTS) as a ?spacer arm? betweenhematin?glutaraldehyde and chitosan was studied.CONCLUSIONS: An economical heterogeneous catalyst alternative to HRP has been obtained. The spacer arm improves thecatalyst ́s performance with activities as high as 57% relative to homogeneous hematin. These results support the notion thatthe activity loss of supported hematin is caused by the restricted access to Fe of the bulky phenolic dyes.
Fil: Córdoba, Agostina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Planta Piloto de Ingeniería Química (I). Grupo Vinculado al Plapiqui - Investigación y Desarrollo en Tecnología Química; Argentina
Fil: Magario, Ivana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Planta Piloto de Ingeniería Química (I). Grupo Vinculado al Plapiqui - Investigación y Desarrollo en Tecnología Química; Argentina
Fil: Ferreira, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
Materia
SUPPORTED HEMATIN
PEROXIDASE
CHITOSAN
ALIZARIN RED S
ACID ORAGEN II
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/102486
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/102486
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Modified chitosan as an economic support for hematin: Application in the decolorization of anthraquinone and azo dyesCórdoba, AgostinaMagario, IvanaFerreira, María LujánSUPPORTED HEMATINPEROXIDASECHITOSANALIZARIN RED SACID ORAGEN IIhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Azo and anthraquinone dyes have been successfully decolorizedusing enzymatic and biomimetic homogeneoussystems.1,2 Hematin, a horseradish peroxidase (HRP) biomimetic, immobilized on chitosan via glutaraldehyde couplingwas ableto decolorize Alizarin Red S and Orange II solutions. A Doehlert experimental design and response surface analysis was appliedto determine conditions for optimalmass of catalyst and catalytic efficiency of the immobilization procedure.RESULTS: The catalysts with supported hematin showed 33% activity (relative to homogeneous hematin as 100%) in decolorizationreactions. After six reuses the catalytic activity was maintained at 60% of the initial one. Hematin anchoring to chitosanwithout alteration of the iron-porphyrin ring was confirmed by ICP, FTIR and UV/visible spectrophotometric methods. Inaddition, the effect on hematin activity in the decolorization of aminopropyltriethoxysilane (APTS) as a ?spacer arm? betweenhematin?glutaraldehyde and chitosan was studied.CONCLUSIONS: An economical heterogeneous catalyst alternative to HRP has been obtained. The spacer arm improves thecatalyst ́s performance with activities as high as 57% relative to homogeneous hematin. These results support the notion thatthe activity loss of supported hematin is caused by the restricted access to Fe of the bulky phenolic dyes.Fil: Córdoba, Agostina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Planta Piloto de Ingeniería Química (I). Grupo Vinculado al Plapiqui - Investigación y Desarrollo en Tecnología Química; ArgentinaFil: Magario, Ivana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Planta Piloto de Ingeniería Química (I). Grupo Vinculado al Plapiqui - Investigación y Desarrollo en Tecnología Química; ArgentinaFil: Ferreira, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; ArgentinaJohn Wiley & Sons Ltd2014-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/102486Córdoba, Agostina; Magario, Ivana; Ferreira, María Luján; Modified chitosan as an economic support for hematin: Application in the decolorization of anthraquinone and azo dyes; John Wiley & Sons Ltd; Journal of Chemical Technology and Biotechnology; 90; 9; 9-2014; 1665-16760268-2575CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jctb.4475info:eu-repo/semantics/altIdentifier/doi/10.1002/jctb.4475info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-26T08:57:52Zoai:ri.conicet.gov.ar:11336/102486instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-26 08:57:52.869CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Modified chitosan as an economic support for hematin: Application in the decolorization of anthraquinone and azo dyes
title Modified chitosan as an economic support for hematin: Application in the decolorization of anthraquinone and azo dyes
spellingShingle Modified chitosan as an economic support for hematin: Application in the decolorization of anthraquinone and azo dyes
Córdoba, Agostina
SUPPORTED HEMATIN
PEROXIDASE
CHITOSAN
ALIZARIN RED S
ACID ORAGEN II
title_short Modified chitosan as an economic support for hematin: Application in the decolorization of anthraquinone and azo dyes
title_full Modified chitosan as an economic support for hematin: Application in the decolorization of anthraquinone and azo dyes
title_fullStr Modified chitosan as an economic support for hematin: Application in the decolorization of anthraquinone and azo dyes
title_full_unstemmed Modified chitosan as an economic support for hematin: Application in the decolorization of anthraquinone and azo dyes
title_sort Modified chitosan as an economic support for hematin: Application in the decolorization of anthraquinone and azo dyes
dc.creator.none.fl_str_mv Córdoba, Agostina
Magario, Ivana
Ferreira, María Luján
author Córdoba, Agostina
author_facet Córdoba, Agostina
Magario, Ivana
Ferreira, María Luján
author_role author
author2 Magario, Ivana
Ferreira, María Luján
author2_role author
author
dc.subject.none.fl_str_mv SUPPORTED HEMATIN
PEROXIDASE
CHITOSAN
ALIZARIN RED S
ACID ORAGEN II
topic SUPPORTED HEMATIN
PEROXIDASE
CHITOSAN
ALIZARIN RED S
ACID ORAGEN II
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Azo and anthraquinone dyes have been successfully decolorizedusing enzymatic and biomimetic homogeneoussystems.1,2 Hematin, a horseradish peroxidase (HRP) biomimetic, immobilized on chitosan via glutaraldehyde couplingwas ableto decolorize Alizarin Red S and Orange II solutions. A Doehlert experimental design and response surface analysis was appliedto determine conditions for optimalmass of catalyst and catalytic efficiency of the immobilization procedure.RESULTS: The catalysts with supported hematin showed 33% activity (relative to homogeneous hematin as 100%) in decolorizationreactions. After six reuses the catalytic activity was maintained at 60% of the initial one. Hematin anchoring to chitosanwithout alteration of the iron-porphyrin ring was confirmed by ICP, FTIR and UV/visible spectrophotometric methods. Inaddition, the effect on hematin activity in the decolorization of aminopropyltriethoxysilane (APTS) as a ?spacer arm? betweenhematin?glutaraldehyde and chitosan was studied.CONCLUSIONS: An economical heterogeneous catalyst alternative to HRP has been obtained. The spacer arm improves thecatalyst ́s performance with activities as high as 57% relative to homogeneous hematin. These results support the notion thatthe activity loss of supported hematin is caused by the restricted access to Fe of the bulky phenolic dyes.
Fil: Córdoba, Agostina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Planta Piloto de Ingeniería Química (I). Grupo Vinculado al Plapiqui - Investigación y Desarrollo en Tecnología Química; Argentina
Fil: Magario, Ivana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Planta Piloto de Ingeniería Química (I). Grupo Vinculado al Plapiqui - Investigación y Desarrollo en Tecnología Química; Argentina
Fil: Ferreira, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
description Azo and anthraquinone dyes have been successfully decolorizedusing enzymatic and biomimetic homogeneoussystems.1,2 Hematin, a horseradish peroxidase (HRP) biomimetic, immobilized on chitosan via glutaraldehyde couplingwas ableto decolorize Alizarin Red S and Orange II solutions. A Doehlert experimental design and response surface analysis was appliedto determine conditions for optimalmass of catalyst and catalytic efficiency of the immobilization procedure.RESULTS: The catalysts with supported hematin showed 33% activity (relative to homogeneous hematin as 100%) in decolorizationreactions. After six reuses the catalytic activity was maintained at 60% of the initial one. Hematin anchoring to chitosanwithout alteration of the iron-porphyrin ring was confirmed by ICP, FTIR and UV/visible spectrophotometric methods. Inaddition, the effect on hematin activity in the decolorization of aminopropyltriethoxysilane (APTS) as a ?spacer arm? betweenhematin?glutaraldehyde and chitosan was studied.CONCLUSIONS: An economical heterogeneous catalyst alternative to HRP has been obtained. The spacer arm improves thecatalyst ́s performance with activities as high as 57% relative to homogeneous hematin. These results support the notion thatthe activity loss of supported hematin is caused by the restricted access to Fe of the bulky phenolic dyes.
publishDate 2014
dc.date.none.fl_str_mv 2014-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/102486
Córdoba, Agostina; Magario, Ivana; Ferreira, María Luján; Modified chitosan as an economic support for hematin: Application in the decolorization of anthraquinone and azo dyes; John Wiley & Sons Ltd; Journal of Chemical Technology and Biotechnology; 90; 9; 9-2014; 1665-1676
0268-2575
CONICET Digital
CONICET
url http://hdl.handle.net/11336/102486
identifier_str_mv Córdoba, Agostina; Magario, Ivana; Ferreira, María Luján; Modified chitosan as an economic support for hematin: Application in the decolorization of anthraquinone and azo dyes; John Wiley & Sons Ltd; Journal of Chemical Technology and Biotechnology; 90; 9; 9-2014; 1665-1676
0268-2575
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jctb.4475
info:eu-repo/semantics/altIdentifier/doi/10.1002/jctb.4475
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons Ltd
publisher.none.fl_str_mv John Wiley & Sons Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.011256

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