Supported biocatalysts for Alizarin and Eriochrome Blue Black R degradation using hydrogen peroxide

Autores
Pirillo, Silvina; Rueda, Elsa Haydee; Ferreira, María Luján
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The goal of this manuscript was to study the immobilization of horseradish peroxidase (HRP) and hematin on different supports at selected conditions to be used in the removal of two synthetic dyes: Alizarin Red (AR) and Eriochrome Blue Black R (EBBR). Reuse in the target reactions was tested for the best biocatalysts. Three supports were selected: magnetite-activated carbon Fe 3O 4/C, activated carbon C and chitosan CS. Several reasons led to discard the use of Fe 3O 4/C and C as supports for hematin and HRP. CS-glutaraldehyde (GA)-HRP not only has low ability to remove EBBR, but also the enzymatic activity of HRP could not be separated from the dye adsorption on CS. However, some adsorptive functions of CS-GA were lost when HRP was supported on it. In the case of CS-GA-hematin, the most important factors for EBBR removal are dye concentration and mass of catalyst, whereas the temperature has almost no effect on the conversion of dye, considering in all cases the H 2O 2 concentration (0.021M) constant. There was not a marked decrease in the removal of AR even after the ninth reuse in the case of CS-GA-hematin. For EBBR removal, with CS-GA-HRP, in the second reuse the catalyst was completely deactivated, whereas with CS-GA-hematin this was found only in the fourth reuse.
Fil: Pirillo, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Rueda, Elsa Haydee. Universidad Nacional del Sur. Departamento de Química; Argentina
Fil: Ferreira, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina. Universidad Nacional del Sur. Departamento de Química; Argentina
Materia
Decolorization
Dyes
Hematin
Horseradish Peroxidase
Immobilized Catalysts
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/54367
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/54367
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Supported biocatalysts for Alizarin and Eriochrome Blue Black R degradation using hydrogen peroxidePirillo, SilvinaRueda, Elsa HaydeeFerreira, María LujánDecolorizationDyesHematinHorseradish PeroxidaseImmobilized Catalystshttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2The goal of this manuscript was to study the immobilization of horseradish peroxidase (HRP) and hematin on different supports at selected conditions to be used in the removal of two synthetic dyes: Alizarin Red (AR) and Eriochrome Blue Black R (EBBR). Reuse in the target reactions was tested for the best biocatalysts. Three supports were selected: magnetite-activated carbon Fe 3O 4/C, activated carbon C and chitosan CS. Several reasons led to discard the use of Fe 3O 4/C and C as supports for hematin and HRP. CS-glutaraldehyde (GA)-HRP not only has low ability to remove EBBR, but also the enzymatic activity of HRP could not be separated from the dye adsorption on CS. However, some adsorptive functions of CS-GA were lost when HRP was supported on it. In the case of CS-GA-hematin, the most important factors for EBBR removal are dye concentration and mass of catalyst, whereas the temperature has almost no effect on the conversion of dye, considering in all cases the H 2O 2 concentration (0.021M) constant. There was not a marked decrease in the removal of AR even after the ninth reuse in the case of CS-GA-hematin. For EBBR removal, with CS-GA-HRP, in the second reuse the catalyst was completely deactivated, whereas with CS-GA-hematin this was found only in the fourth reuse.Fil: Pirillo, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Rueda, Elsa Haydee. Universidad Nacional del Sur. Departamento de Química; ArgentinaFil: Ferreira, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina. Universidad Nacional del Sur. Departamento de Química; ArgentinaElsevier Science Sa2012-09-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/54367Pirillo, Silvina; Rueda, Elsa Haydee; Ferreira, María Luján; Supported biocatalysts for Alizarin and Eriochrome Blue Black R degradation using hydrogen peroxide; Elsevier Science Sa; Chemical Engineering Journal; 204-206; 15-9-2012; 65-711385-8947CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1385894712009485info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cej.2012.07.057info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:09:52Zoai:ri.conicet.gov.ar:11336/54367instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:09:53.2CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Supported biocatalysts for Alizarin and Eriochrome Blue Black R degradation using hydrogen peroxide
title Supported biocatalysts for Alizarin and Eriochrome Blue Black R degradation using hydrogen peroxide
spellingShingle Supported biocatalysts for Alizarin and Eriochrome Blue Black R degradation using hydrogen peroxide
Pirillo, Silvina
Decolorization
Dyes
Hematin
Horseradish Peroxidase
Immobilized Catalysts
title_short Supported biocatalysts for Alizarin and Eriochrome Blue Black R degradation using hydrogen peroxide
title_full Supported biocatalysts for Alizarin and Eriochrome Blue Black R degradation using hydrogen peroxide
title_fullStr Supported biocatalysts for Alizarin and Eriochrome Blue Black R degradation using hydrogen peroxide
title_full_unstemmed Supported biocatalysts for Alizarin and Eriochrome Blue Black R degradation using hydrogen peroxide
title_sort Supported biocatalysts for Alizarin and Eriochrome Blue Black R degradation using hydrogen peroxide
dc.creator.none.fl_str_mv Pirillo, Silvina
Rueda, Elsa Haydee
Ferreira, María Luján
author Pirillo, Silvina
author_facet Pirillo, Silvina
Rueda, Elsa Haydee
Ferreira, María Luján
author_role author
author2 Rueda, Elsa Haydee
Ferreira, María Luján
author2_role author
author
dc.subject.none.fl_str_mv Decolorization
Dyes
Hematin
Horseradish Peroxidase
Immobilized Catalysts
topic Decolorization
Dyes
Hematin
Horseradish Peroxidase
Immobilized Catalysts
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The goal of this manuscript was to study the immobilization of horseradish peroxidase (HRP) and hematin on different supports at selected conditions to be used in the removal of two synthetic dyes: Alizarin Red (AR) and Eriochrome Blue Black R (EBBR). Reuse in the target reactions was tested for the best biocatalysts. Three supports were selected: magnetite-activated carbon Fe 3O 4/C, activated carbon C and chitosan CS. Several reasons led to discard the use of Fe 3O 4/C and C as supports for hematin and HRP. CS-glutaraldehyde (GA)-HRP not only has low ability to remove EBBR, but also the enzymatic activity of HRP could not be separated from the dye adsorption on CS. However, some adsorptive functions of CS-GA were lost when HRP was supported on it. In the case of CS-GA-hematin, the most important factors for EBBR removal are dye concentration and mass of catalyst, whereas the temperature has almost no effect on the conversion of dye, considering in all cases the H 2O 2 concentration (0.021M) constant. There was not a marked decrease in the removal of AR even after the ninth reuse in the case of CS-GA-hematin. For EBBR removal, with CS-GA-HRP, in the second reuse the catalyst was completely deactivated, whereas with CS-GA-hematin this was found only in the fourth reuse.
Fil: Pirillo, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Rueda, Elsa Haydee. Universidad Nacional del Sur. Departamento de Química; Argentina
Fil: Ferreira, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina. Universidad Nacional del Sur. Departamento de Química; Argentina
description The goal of this manuscript was to study the immobilization of horseradish peroxidase (HRP) and hematin on different supports at selected conditions to be used in the removal of two synthetic dyes: Alizarin Red (AR) and Eriochrome Blue Black R (EBBR). Reuse in the target reactions was tested for the best biocatalysts. Three supports were selected: magnetite-activated carbon Fe 3O 4/C, activated carbon C and chitosan CS. Several reasons led to discard the use of Fe 3O 4/C and C as supports for hematin and HRP. CS-glutaraldehyde (GA)-HRP not only has low ability to remove EBBR, but also the enzymatic activity of HRP could not be separated from the dye adsorption on CS. However, some adsorptive functions of CS-GA were lost when HRP was supported on it. In the case of CS-GA-hematin, the most important factors for EBBR removal are dye concentration and mass of catalyst, whereas the temperature has almost no effect on the conversion of dye, considering in all cases the H 2O 2 concentration (0.021M) constant. There was not a marked decrease in the removal of AR even after the ninth reuse in the case of CS-GA-hematin. For EBBR removal, with CS-GA-HRP, in the second reuse the catalyst was completely deactivated, whereas with CS-GA-hematin this was found only in the fourth reuse.
publishDate 2012
dc.date.none.fl_str_mv 2012-09-15
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/54367
Pirillo, Silvina; Rueda, Elsa Haydee; Ferreira, María Luján; Supported biocatalysts for Alizarin and Eriochrome Blue Black R degradation using hydrogen peroxide; Elsevier Science Sa; Chemical Engineering Journal; 204-206; 15-9-2012; 65-71
1385-8947
CONICET Digital
CONICET
url http://hdl.handle.net/11336/54367
identifier_str_mv Pirillo, Silvina; Rueda, Elsa Haydee; Ferreira, María Luján; Supported biocatalysts for Alizarin and Eriochrome Blue Black R degradation using hydrogen peroxide; Elsevier Science Sa; Chemical Engineering Journal; 204-206; 15-9-2012; 65-71
1385-8947
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1385894712009485
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cej.2012.07.057
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Sa
publisher.none.fl_str_mv Elsevier Science Sa
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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