Supported biocatalysts for Alizarin and Eriochrome Blue Black R degradation using hydrogen peroxide
- Autores
- Pirillo, Silvina; Rueda, Elsa Haydee; Ferreira, María Luján
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The goal of this manuscript was to study the immobilization of horseradish peroxidase (HRP) and hematin on different supports at selected conditions to be used in the removal of two synthetic dyes: Alizarin Red (AR) and Eriochrome Blue Black R (EBBR). Reuse in the target reactions was tested for the best biocatalysts. Three supports were selected: magnetite-activated carbon Fe 3O 4/C, activated carbon C and chitosan CS. Several reasons led to discard the use of Fe 3O 4/C and C as supports for hematin and HRP. CS-glutaraldehyde (GA)-HRP not only has low ability to remove EBBR, but also the enzymatic activity of HRP could not be separated from the dye adsorption on CS. However, some adsorptive functions of CS-GA were lost when HRP was supported on it. In the case of CS-GA-hematin, the most important factors for EBBR removal are dye concentration and mass of catalyst, whereas the temperature has almost no effect on the conversion of dye, considering in all cases the H 2O 2 concentration (0.021M) constant. There was not a marked decrease in the removal of AR even after the ninth reuse in the case of CS-GA-hematin. For EBBR removal, with CS-GA-HRP, in the second reuse the catalyst was completely deactivated, whereas with CS-GA-hematin this was found only in the fourth reuse.
Fil: Pirillo, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Rueda, Elsa Haydee. Universidad Nacional del Sur. Departamento de Química; Argentina
Fil: Ferreira, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina. Universidad Nacional del Sur. Departamento de Química; Argentina - Materia
-
Decolorization
Dyes
Hematin
Horseradish Peroxidase
Immobilized Catalysts - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/54367
Ver los metadatos del registro completo
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Supported biocatalysts for Alizarin and Eriochrome Blue Black R degradation using hydrogen peroxidePirillo, SilvinaRueda, Elsa HaydeeFerreira, María LujánDecolorizationDyesHematinHorseradish PeroxidaseImmobilized Catalystshttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2The goal of this manuscript was to study the immobilization of horseradish peroxidase (HRP) and hematin on different supports at selected conditions to be used in the removal of two synthetic dyes: Alizarin Red (AR) and Eriochrome Blue Black R (EBBR). Reuse in the target reactions was tested for the best biocatalysts. Three supports were selected: magnetite-activated carbon Fe 3O 4/C, activated carbon C and chitosan CS. Several reasons led to discard the use of Fe 3O 4/C and C as supports for hematin and HRP. CS-glutaraldehyde (GA)-HRP not only has low ability to remove EBBR, but also the enzymatic activity of HRP could not be separated from the dye adsorption on CS. However, some adsorptive functions of CS-GA were lost when HRP was supported on it. In the case of CS-GA-hematin, the most important factors for EBBR removal are dye concentration and mass of catalyst, whereas the temperature has almost no effect on the conversion of dye, considering in all cases the H 2O 2 concentration (0.021M) constant. There was not a marked decrease in the removal of AR even after the ninth reuse in the case of CS-GA-hematin. For EBBR removal, with CS-GA-HRP, in the second reuse the catalyst was completely deactivated, whereas with CS-GA-hematin this was found only in the fourth reuse.Fil: Pirillo, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Rueda, Elsa Haydee. Universidad Nacional del Sur. Departamento de Química; ArgentinaFil: Ferreira, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina. Universidad Nacional del Sur. Departamento de Química; ArgentinaElsevier Science Sa2012-09-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/54367Pirillo, Silvina; Rueda, Elsa Haydee; Ferreira, María Luján; Supported biocatalysts for Alizarin and Eriochrome Blue Black R degradation using hydrogen peroxide; Elsevier Science Sa; Chemical Engineering Journal; 204-206; 15-9-2012; 65-711385-8947CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1385894712009485info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cej.2012.07.057info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:37:44Zoai:ri.conicet.gov.ar:11336/54367instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:37:45.008CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Supported biocatalysts for Alizarin and Eriochrome Blue Black R degradation using hydrogen peroxide |
title |
Supported biocatalysts for Alizarin and Eriochrome Blue Black R degradation using hydrogen peroxide |
spellingShingle |
Supported biocatalysts for Alizarin and Eriochrome Blue Black R degradation using hydrogen peroxide Pirillo, Silvina Decolorization Dyes Hematin Horseradish Peroxidase Immobilized Catalysts |
title_short |
Supported biocatalysts for Alizarin and Eriochrome Blue Black R degradation using hydrogen peroxide |
title_full |
Supported biocatalysts for Alizarin and Eriochrome Blue Black R degradation using hydrogen peroxide |
title_fullStr |
Supported biocatalysts for Alizarin and Eriochrome Blue Black R degradation using hydrogen peroxide |
title_full_unstemmed |
Supported biocatalysts for Alizarin and Eriochrome Blue Black R degradation using hydrogen peroxide |
title_sort |
Supported biocatalysts for Alizarin and Eriochrome Blue Black R degradation using hydrogen peroxide |
dc.creator.none.fl_str_mv |
Pirillo, Silvina Rueda, Elsa Haydee Ferreira, María Luján |
author |
Pirillo, Silvina |
author_facet |
Pirillo, Silvina Rueda, Elsa Haydee Ferreira, María Luján |
author_role |
author |
author2 |
Rueda, Elsa Haydee Ferreira, María Luján |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Decolorization Dyes Hematin Horseradish Peroxidase Immobilized Catalysts |
topic |
Decolorization Dyes Hematin Horseradish Peroxidase Immobilized Catalysts |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.8 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
The goal of this manuscript was to study the immobilization of horseradish peroxidase (HRP) and hematin on different supports at selected conditions to be used in the removal of two synthetic dyes: Alizarin Red (AR) and Eriochrome Blue Black R (EBBR). Reuse in the target reactions was tested for the best biocatalysts. Three supports were selected: magnetite-activated carbon Fe 3O 4/C, activated carbon C and chitosan CS. Several reasons led to discard the use of Fe 3O 4/C and C as supports for hematin and HRP. CS-glutaraldehyde (GA)-HRP not only has low ability to remove EBBR, but also the enzymatic activity of HRP could not be separated from the dye adsorption on CS. However, some adsorptive functions of CS-GA were lost when HRP was supported on it. In the case of CS-GA-hematin, the most important factors for EBBR removal are dye concentration and mass of catalyst, whereas the temperature has almost no effect on the conversion of dye, considering in all cases the H 2O 2 concentration (0.021M) constant. There was not a marked decrease in the removal of AR even after the ninth reuse in the case of CS-GA-hematin. For EBBR removal, with CS-GA-HRP, in the second reuse the catalyst was completely deactivated, whereas with CS-GA-hematin this was found only in the fourth reuse. Fil: Pirillo, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina Fil: Rueda, Elsa Haydee. Universidad Nacional del Sur. Departamento de Química; Argentina Fil: Ferreira, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina. Universidad Nacional del Sur. Departamento de Química; Argentina |
description |
The goal of this manuscript was to study the immobilization of horseradish peroxidase (HRP) and hematin on different supports at selected conditions to be used in the removal of two synthetic dyes: Alizarin Red (AR) and Eriochrome Blue Black R (EBBR). Reuse in the target reactions was tested for the best biocatalysts. Three supports were selected: magnetite-activated carbon Fe 3O 4/C, activated carbon C and chitosan CS. Several reasons led to discard the use of Fe 3O 4/C and C as supports for hematin and HRP. CS-glutaraldehyde (GA)-HRP not only has low ability to remove EBBR, but also the enzymatic activity of HRP could not be separated from the dye adsorption on CS. However, some adsorptive functions of CS-GA were lost when HRP was supported on it. In the case of CS-GA-hematin, the most important factors for EBBR removal are dye concentration and mass of catalyst, whereas the temperature has almost no effect on the conversion of dye, considering in all cases the H 2O 2 concentration (0.021M) constant. There was not a marked decrease in the removal of AR even after the ninth reuse in the case of CS-GA-hematin. For EBBR removal, with CS-GA-HRP, in the second reuse the catalyst was completely deactivated, whereas with CS-GA-hematin this was found only in the fourth reuse. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-09-15 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/54367 Pirillo, Silvina; Rueda, Elsa Haydee; Ferreira, María Luján; Supported biocatalysts for Alizarin and Eriochrome Blue Black R degradation using hydrogen peroxide; Elsevier Science Sa; Chemical Engineering Journal; 204-206; 15-9-2012; 65-71 1385-8947 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/54367 |
identifier_str_mv |
Pirillo, Silvina; Rueda, Elsa Haydee; Ferreira, María Luján; Supported biocatalysts for Alizarin and Eriochrome Blue Black R degradation using hydrogen peroxide; Elsevier Science Sa; Chemical Engineering Journal; 204-206; 15-9-2012; 65-71 1385-8947 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1385894712009485 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cej.2012.07.057 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science Sa |
publisher.none.fl_str_mv |
Elsevier Science Sa |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614398380867584 |
score |
13.070432 |