Effect of different orgaphosphates on the hepatic oxidative metabolism by mixed function oxidases in cattle

Autores
Larsen, Karen Elizabeth; Lifschitz, Adrian Luis; Virkel, Guillermo Leon
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Organophosphates (OPs) are widely used for crop protection in agriculture and for the control of ectoparasites in animal husbandry. The sustained use of these chemical compounds increases the risk of environmental contamination and/or alteration of different physiological cellular functions in farm animals. A number of OPs are substrates of hepatic mixed function oxidases, such as those belonging to the cytochrome P450 (CYP) and flavin-containing monooxygenase (FMO) families of enzymes. In addition, these xenobiotics may also affect enzyme function by induction or inhibition of their catalytic activities. This work aimed to evaluate in vitro the effect of the following OPs: chlorpyrifos (CPF), ethion (ETN), diazinon (DZN) and dichlorvos (DCV) on CYP- and FMO-dependent enzyme activities in cattle liver. Bovine (n=4) liver microsomes were incubated (10 min at 37°C in aerobiosis) in the absence (control assays) and in presence of each OP compound under study at 1, 10 and 100 µM (final concentrations). Five CYP- or FMO-dependent catalytic activities were assayed by spectrofluorimetric or HPLC methods: 7-ethoxyresorufin O-deethylase (EROD, for CYP1A1), methoxyresorufin O-demethylase (MROD, for CYP1A2), benzyloxyresorufin O-debenzylase (BROD, for CYP2B), testosterone 6-beta hydroxylase (for CYP3A) and benzydamine N-oxidase (for FMO). Only the CYP3A-dependent hepatic metabolism was significantly affected by the presence of ETN and DZN. ETN, at 10 µM and 100 µM, inhibited (p<0.01) testosterone 6-beta hydroxylase activity (76% and 81%, respectively) in cattle liver microsomes. Similar results were obtained in presence of equimolar concentrations of DZN (74% and 93% at 10 uM and 100 µM, respectively; p<0.01). Both ETN and DZN would potentially interfere with the pattern of the hepatic metabolism of relevant CYP3A substrates pharmacologically relevant in bovine medicine, such as tiamulin, macrolide antibiotics and the ionophore monensin.
Fil: Larsen, Karen Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Ecotoxicología; Argentina
Fil: Lifschitz, Adrian Luis. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Virkel, Guillermo Leon. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina
LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC); LXIX Reunión Anual de la Sociedad Argentina de Inmunología (SAI); LIII Reunión Anual de la Asociación Argentina de Farmacología Experimental (AAFE) y XI Reunión Anual de la Asociación Argentina de Nanomedicinas (NANOMED-AR)
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Asociación Argentina de Farmacología Experimental
Asociación Argentina de Nanomedicinas
Materia
ORGANOPHOSPHATES
CYTOCHROME P450
FLAVIN-CONTAINING MONOOXYGENASE
LIVER
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/156074
Acceder
id CONICETDig_ac87a8f5a2c258a2d52f2f7308b348ae
oai_identifier_str oai:ri.conicet.gov.ar:11336/156074
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of different orgaphosphates on the hepatic oxidative metabolism by mixed function oxidases in cattleLarsen, Karen ElizabethLifschitz, Adrian LuisVirkel, Guillermo LeonORGANOPHOSPHATESCYTOCHROME P450FLAVIN-CONTAINING MONOOXYGENASELIVERhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Organophosphates (OPs) are widely used for crop protection in agriculture and for the control of ectoparasites in animal husbandry. The sustained use of these chemical compounds increases the risk of environmental contamination and/or alteration of different physiological cellular functions in farm animals. A number of OPs are substrates of hepatic mixed function oxidases, such as those belonging to the cytochrome P450 (CYP) and flavin-containing monooxygenase (FMO) families of enzymes. In addition, these xenobiotics may also affect enzyme function by induction or inhibition of their catalytic activities. This work aimed to evaluate in vitro the effect of the following OPs: chlorpyrifos (CPF), ethion (ETN), diazinon (DZN) and dichlorvos (DCV) on CYP- and FMO-dependent enzyme activities in cattle liver. Bovine (n=4) liver microsomes were incubated (10 min at 37°C in aerobiosis) in the absence (control assays) and in presence of each OP compound under study at 1, 10 and 100 µM (final concentrations). Five CYP- or FMO-dependent catalytic activities were assayed by spectrofluorimetric or HPLC methods: 7-ethoxyresorufin O-deethylase (EROD, for CYP1A1), methoxyresorufin O-demethylase (MROD, for CYP1A2), benzyloxyresorufin O-debenzylase (BROD, for CYP2B), testosterone 6-beta hydroxylase (for CYP3A) and benzydamine N-oxidase (for FMO). Only the CYP3A-dependent hepatic metabolism was significantly affected by the presence of ETN and DZN. ETN, at 10 µM and 100 µM, inhibited (p<0.01) testosterone 6-beta hydroxylase activity (76% and 81%, respectively) in cattle liver microsomes. Similar results were obtained in presence of equimolar concentrations of DZN (74% and 93% at 10 uM and 100 µM, respectively; p<0.01). Both ETN and DZN would potentially interfere with the pattern of the hepatic metabolism of relevant CYP3A substrates pharmacologically relevant in bovine medicine, such as tiamulin, macrolide antibiotics and the ionophore monensin.Fil: Larsen, Karen Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Ecotoxicología; ArgentinaFil: Lifschitz, Adrian Luis. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Virkel, Guillermo Leon. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; ArgentinaLXVI Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC); LXIX Reunión Anual de la Sociedad Argentina de Inmunología (SAI); LIII Reunión Anual de la Asociación Argentina de Farmacología Experimental (AAFE) y XI Reunión Anual de la Asociación Argentina de Nanomedicinas (NANOMED-AR)ArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de InmunologíaAsociación Argentina de Farmacología ExperimentalAsociación Argentina de NanomedicinasFundación Revista Medicina2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/156074Effect of different orgaphosphates on the hepatic oxidative metabolism by mixed function oxidases in cattle; LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC); LXIX Reunión Anual de la Sociedad Argentina de Inmunología (SAI); LIII Reunión Anual de la Asociación Argentina de Farmacología Experimental (AAFE) y XI Reunión Anual de la Asociación Argentina de Nanomedicinas (NANOMED-AR); Argentina; 2021; 94-951669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol81-21/s3/Mv81s3.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:10:14Zoai:ri.conicet.gov.ar:11336/156074instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:10:15.048CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of different orgaphosphates on the hepatic oxidative metabolism by mixed function oxidases in cattle
title Effect of different orgaphosphates on the hepatic oxidative metabolism by mixed function oxidases in cattle
spellingShingle Effect of different orgaphosphates on the hepatic oxidative metabolism by mixed function oxidases in cattle
Larsen, Karen Elizabeth
ORGANOPHOSPHATES
CYTOCHROME P450
FLAVIN-CONTAINING MONOOXYGENASE
LIVER
title_short Effect of different orgaphosphates on the hepatic oxidative metabolism by mixed function oxidases in cattle
title_full Effect of different orgaphosphates on the hepatic oxidative metabolism by mixed function oxidases in cattle
title_fullStr Effect of different orgaphosphates on the hepatic oxidative metabolism by mixed function oxidases in cattle
title_full_unstemmed Effect of different orgaphosphates on the hepatic oxidative metabolism by mixed function oxidases in cattle
title_sort Effect of different orgaphosphates on the hepatic oxidative metabolism by mixed function oxidases in cattle
dc.creator.none.fl_str_mv Larsen, Karen Elizabeth
Lifschitz, Adrian Luis
Virkel, Guillermo Leon
author Larsen, Karen Elizabeth
author_facet Larsen, Karen Elizabeth
Lifschitz, Adrian Luis
Virkel, Guillermo Leon
author_role author
author2 Lifschitz, Adrian Luis
Virkel, Guillermo Leon
author2_role author
author
dc.subject.none.fl_str_mv ORGANOPHOSPHATES
CYTOCHROME P450
FLAVIN-CONTAINING MONOOXYGENASE
LIVER
topic ORGANOPHOSPHATES
CYTOCHROME P450
FLAVIN-CONTAINING MONOOXYGENASE
LIVER
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Organophosphates (OPs) are widely used for crop protection in agriculture and for the control of ectoparasites in animal husbandry. The sustained use of these chemical compounds increases the risk of environmental contamination and/or alteration of different physiological cellular functions in farm animals. A number of OPs are substrates of hepatic mixed function oxidases, such as those belonging to the cytochrome P450 (CYP) and flavin-containing monooxygenase (FMO) families of enzymes. In addition, these xenobiotics may also affect enzyme function by induction or inhibition of their catalytic activities. This work aimed to evaluate in vitro the effect of the following OPs: chlorpyrifos (CPF), ethion (ETN), diazinon (DZN) and dichlorvos (DCV) on CYP- and FMO-dependent enzyme activities in cattle liver. Bovine (n=4) liver microsomes were incubated (10 min at 37°C in aerobiosis) in the absence (control assays) and in presence of each OP compound under study at 1, 10 and 100 µM (final concentrations). Five CYP- or FMO-dependent catalytic activities were assayed by spectrofluorimetric or HPLC methods: 7-ethoxyresorufin O-deethylase (EROD, for CYP1A1), methoxyresorufin O-demethylase (MROD, for CYP1A2), benzyloxyresorufin O-debenzylase (BROD, for CYP2B), testosterone 6-beta hydroxylase (for CYP3A) and benzydamine N-oxidase (for FMO). Only the CYP3A-dependent hepatic metabolism was significantly affected by the presence of ETN and DZN. ETN, at 10 µM and 100 µM, inhibited (p<0.01) testosterone 6-beta hydroxylase activity (76% and 81%, respectively) in cattle liver microsomes. Similar results were obtained in presence of equimolar concentrations of DZN (74% and 93% at 10 uM and 100 µM, respectively; p<0.01). Both ETN and DZN would potentially interfere with the pattern of the hepatic metabolism of relevant CYP3A substrates pharmacologically relevant in bovine medicine, such as tiamulin, macrolide antibiotics and the ionophore monensin.
Fil: Larsen, Karen Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Ecotoxicología; Argentina
Fil: Lifschitz, Adrian Luis. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Virkel, Guillermo Leon. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina
LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC); LXIX Reunión Anual de la Sociedad Argentina de Inmunología (SAI); LIII Reunión Anual de la Asociación Argentina de Farmacología Experimental (AAFE) y XI Reunión Anual de la Asociación Argentina de Nanomedicinas (NANOMED-AR)
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Asociación Argentina de Farmacología Experimental
Asociación Argentina de Nanomedicinas
description Organophosphates (OPs) are widely used for crop protection in agriculture and for the control of ectoparasites in animal husbandry. The sustained use of these chemical compounds increases the risk of environmental contamination and/or alteration of different physiological cellular functions in farm animals. A number of OPs are substrates of hepatic mixed function oxidases, such as those belonging to the cytochrome P450 (CYP) and flavin-containing monooxygenase (FMO) families of enzymes. In addition, these xenobiotics may also affect enzyme function by induction or inhibition of their catalytic activities. This work aimed to evaluate in vitro the effect of the following OPs: chlorpyrifos (CPF), ethion (ETN), diazinon (DZN) and dichlorvos (DCV) on CYP- and FMO-dependent enzyme activities in cattle liver. Bovine (n=4) liver microsomes were incubated (10 min at 37°C in aerobiosis) in the absence (control assays) and in presence of each OP compound under study at 1, 10 and 100 µM (final concentrations). Five CYP- or FMO-dependent catalytic activities were assayed by spectrofluorimetric or HPLC methods: 7-ethoxyresorufin O-deethylase (EROD, for CYP1A1), methoxyresorufin O-demethylase (MROD, for CYP1A2), benzyloxyresorufin O-debenzylase (BROD, for CYP2B), testosterone 6-beta hydroxylase (for CYP3A) and benzydamine N-oxidase (for FMO). Only the CYP3A-dependent hepatic metabolism was significantly affected by the presence of ETN and DZN. ETN, at 10 µM and 100 µM, inhibited (p<0.01) testosterone 6-beta hydroxylase activity (76% and 81%, respectively) in cattle liver microsomes. Similar results were obtained in presence of equimolar concentrations of DZN (74% and 93% at 10 uM and 100 µM, respectively; p<0.01). Both ETN and DZN would potentially interfere with the pattern of the hepatic metabolism of relevant CYP3A substrates pharmacologically relevant in bovine medicine, such as tiamulin, macrolide antibiotics and the ionophore monensin.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/156074
Effect of different orgaphosphates on the hepatic oxidative metabolism by mixed function oxidases in cattle; LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC); LXIX Reunión Anual de la Sociedad Argentina de Inmunología (SAI); LIII Reunión Anual de la Asociación Argentina de Farmacología Experimental (AAFE) y XI Reunión Anual de la Asociación Argentina de Nanomedicinas (NANOMED-AR); Argentina; 2021; 94-95
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/156074
identifier_str_mv Effect of different orgaphosphates on the hepatic oxidative metabolism by mixed function oxidases in cattle; LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC); LXIX Reunión Anual de la Sociedad Argentina de Inmunología (SAI); LIII Reunión Anual de la Asociación Argentina de Farmacología Experimental (AAFE) y XI Reunión Anual de la Asociación Argentina de Nanomedicinas (NANOMED-AR); Argentina; 2021; 94-95
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol81-21/s3/Mv81s3.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842980513545977856
score 12.993085

Publicaciones similares