Molecular Mechanisms of Resistance to Ceftazidime/Avibactam in Clinical Isolates of Enterobacterales and Pseudomonas aeruginosa in Latin American Hospitals
- Autores
- Mojica, María Fernanda; De La Cadena, Elsa; García Betancur, Juan Carlos; Porras, Jessica; Novoa Caicedo, Isabella; Páez Zamora, Laura; Pallares, Christian; Appel, Tobias Manuel; Radice, Marcela Alejandra; Castañeda Méndez, Paulo; Gales, Ana C.; Munita, José M.; Villegas, María Virginia
- Año de publicación
- 2023
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Ceftazidime-avibactam (CZA) is the combination of a third-generation cephalosporin and a new non-b-lactam b-lactamase inhibitor capable of inactivating class A, C, and some D b-lactamases. From a collection of 2,727 clinical isolates of Enterobacterales (n = 2,235) and P. aeruginosa (n = 492) that were collected between 2016 and 2017 from five Latin American countries, we investigated the molecular resistance mechanisms to CZA of 127 (18/2,235 [0.8%] Enterobacterales and 109/492 [22.1%] P. aeruginosa). First, by qPCR for the presence of genes encoding KPC, NDM, VIM, IMP, OXA-48-like, and SPM-1 carbapenemases, and second, by whole-genome sequencing (WGS). From the CZA-resistant isolates, MBL-encoding genes were detected in all 18 Enterobacterales and 42/109 P. aeruginosa isolates, explaining their resistant phenotype. Resistant isolates that yielded a negative qPCR result for any of the MBL encoding genes were subjected to WGS. The WGS analysis of the 67 remaining P. aeruginosa isolates showed mutations in genes previously associated with reduced susceptibility to CZA, such as those involved in the MexAB-OprM efflux pump and AmpC (PDC) hyperproduction, PoxB (blaOXA-50-like), FtsI (PBP3), DacB (PBP4), and OprD. The results presented here offer a snapshot of the molecular epidemiological landscape for CZA resistance before the introduction of this antibiotic into the Latin American market. Therefore, these results serve as a valuable comparison tool to trace the evolution of the resistance to CZA in this carbapenemase-endemic geographical region.
Fil: Mojica, María Fernanda. Case Western Reserve University; Estados Unidos. Va Northeast Ohio Healthcare System; Estados Unidos. Universidad El Bosque;
Fil: De La Cadena, Elsa. Universidad El Bosque;
Fil: García Betancur, Juan Carlos. Universidad El Bosque;
Fil: Porras, Jessica. Universidad El Bosque;
Fil: Novoa Caicedo, Isabella. Universidad El Bosque;
Fil: Páez Zamora, Laura. Universidad El Bosque;
Fil: Pallares, Christian. Universidad El Bosque;
Fil: Appel, Tobias Manuel. Universidad El Bosque;
Fil: Radice, Marcela Alejandra. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Castañeda Méndez, Paulo. Hospital San Ángel Inn Universidad; México. Fundacion Clinica Medica Sur; México
Fil: Gales, Ana C.. Universidade Federal de Sao Paulo; Brasil
Fil: Munita, José M.. Millennium Initiative for Collaborative Research on Bacterial Resistance; Chile
Fil: Villegas, María Virginia. Universidad El Bosque; - Materia
-
ANTIMICROBIAL RESISTANCE
CEFTAZIDIME/AVIBACTAM
ENTEROBACTERALES
LATIN AMERICA
PSEUDOMONAS AERUGINOSA - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/227554
Ver los metadatos del registro completo
id |
CONICETDig_ac77ce2f9e93dbf198bc7296286c1096 |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/227554 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Molecular Mechanisms of Resistance to Ceftazidime/Avibactam in Clinical Isolates of Enterobacterales and Pseudomonas aeruginosa in Latin American HospitalsMojica, María FernandaDe La Cadena, ElsaGarcía Betancur, Juan CarlosPorras, JessicaNovoa Caicedo, IsabellaPáez Zamora, LauraPallares, ChristianAppel, Tobias ManuelRadice, Marcela AlejandraCastañeda Méndez, PauloGales, Ana C.Munita, José M.Villegas, María VirginiaANTIMICROBIAL RESISTANCECEFTAZIDIME/AVIBACTAMENTEROBACTERALESLATIN AMERICAPSEUDOMONAS AERUGINOSAhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Ceftazidime-avibactam (CZA) is the combination of a third-generation cephalosporin and a new non-b-lactam b-lactamase inhibitor capable of inactivating class A, C, and some D b-lactamases. From a collection of 2,727 clinical isolates of Enterobacterales (n = 2,235) and P. aeruginosa (n = 492) that were collected between 2016 and 2017 from five Latin American countries, we investigated the molecular resistance mechanisms to CZA of 127 (18/2,235 [0.8%] Enterobacterales and 109/492 [22.1%] P. aeruginosa). First, by qPCR for the presence of genes encoding KPC, NDM, VIM, IMP, OXA-48-like, and SPM-1 carbapenemases, and second, by whole-genome sequencing (WGS). From the CZA-resistant isolates, MBL-encoding genes were detected in all 18 Enterobacterales and 42/109 P. aeruginosa isolates, explaining their resistant phenotype. Resistant isolates that yielded a negative qPCR result for any of the MBL encoding genes were subjected to WGS. The WGS analysis of the 67 remaining P. aeruginosa isolates showed mutations in genes previously associated with reduced susceptibility to CZA, such as those involved in the MexAB-OprM efflux pump and AmpC (PDC) hyperproduction, PoxB (blaOXA-50-like), FtsI (PBP3), DacB (PBP4), and OprD. The results presented here offer a snapshot of the molecular epidemiological landscape for CZA resistance before the introduction of this antibiotic into the Latin American market. Therefore, these results serve as a valuable comparison tool to trace the evolution of the resistance to CZA in this carbapenemase-endemic geographical region.Fil: Mojica, María Fernanda. Case Western Reserve University; Estados Unidos. Va Northeast Ohio Healthcare System; Estados Unidos. Universidad El Bosque;Fil: De La Cadena, Elsa. Universidad El Bosque;Fil: García Betancur, Juan Carlos. Universidad El Bosque;Fil: Porras, Jessica. Universidad El Bosque;Fil: Novoa Caicedo, Isabella. Universidad El Bosque;Fil: Páez Zamora, Laura. Universidad El Bosque;Fil: Pallares, Christian. Universidad El Bosque;Fil: Appel, Tobias Manuel. Universidad El Bosque;Fil: Radice, Marcela Alejandra. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Castañeda Méndez, Paulo. Hospital San Ángel Inn Universidad; México. Fundacion Clinica Medica Sur; MéxicoFil: Gales, Ana C.. Universidade Federal de Sao Paulo; BrasilFil: Munita, José M.. Millennium Initiative for Collaborative Research on Bacterial Resistance; ChileFil: Villegas, María Virginia. Universidad El Bosque;American Society for Microbiology2023-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/227554Mojica, María Fernanda; De La Cadena, Elsa; García Betancur, Juan Carlos; Porras, Jessica; Novoa Caicedo, Isabella; et al.; Molecular Mechanisms of Resistance to Ceftazidime/Avibactam in Clinical Isolates of Enterobacterales and Pseudomonas aeruginosa in Latin American Hospitals; American Society for Microbiology; mSphere; 8; 2; 4-2023; 1-102379-5042CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1128/msphere.00651-22info:eu-repo/semantics/altIdentifier/url/https://journals.asm.org/doi/10.1128/msphere.00651-22info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:31:10Zoai:ri.conicet.gov.ar:11336/227554instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:31:11.13CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Molecular Mechanisms of Resistance to Ceftazidime/Avibactam in Clinical Isolates of Enterobacterales and Pseudomonas aeruginosa in Latin American Hospitals |
title |
Molecular Mechanisms of Resistance to Ceftazidime/Avibactam in Clinical Isolates of Enterobacterales and Pseudomonas aeruginosa in Latin American Hospitals |
spellingShingle |
Molecular Mechanisms of Resistance to Ceftazidime/Avibactam in Clinical Isolates of Enterobacterales and Pseudomonas aeruginosa in Latin American Hospitals Mojica, María Fernanda ANTIMICROBIAL RESISTANCE CEFTAZIDIME/AVIBACTAM ENTEROBACTERALES LATIN AMERICA PSEUDOMONAS AERUGINOSA |
title_short |
Molecular Mechanisms of Resistance to Ceftazidime/Avibactam in Clinical Isolates of Enterobacterales and Pseudomonas aeruginosa in Latin American Hospitals |
title_full |
Molecular Mechanisms of Resistance to Ceftazidime/Avibactam in Clinical Isolates of Enterobacterales and Pseudomonas aeruginosa in Latin American Hospitals |
title_fullStr |
Molecular Mechanisms of Resistance to Ceftazidime/Avibactam in Clinical Isolates of Enterobacterales and Pseudomonas aeruginosa in Latin American Hospitals |
title_full_unstemmed |
Molecular Mechanisms of Resistance to Ceftazidime/Avibactam in Clinical Isolates of Enterobacterales and Pseudomonas aeruginosa in Latin American Hospitals |
title_sort |
Molecular Mechanisms of Resistance to Ceftazidime/Avibactam in Clinical Isolates of Enterobacterales and Pseudomonas aeruginosa in Latin American Hospitals |
dc.creator.none.fl_str_mv |
Mojica, María Fernanda De La Cadena, Elsa García Betancur, Juan Carlos Porras, Jessica Novoa Caicedo, Isabella Páez Zamora, Laura Pallares, Christian Appel, Tobias Manuel Radice, Marcela Alejandra Castañeda Méndez, Paulo Gales, Ana C. Munita, José M. Villegas, María Virginia |
author |
Mojica, María Fernanda |
author_facet |
Mojica, María Fernanda De La Cadena, Elsa García Betancur, Juan Carlos Porras, Jessica Novoa Caicedo, Isabella Páez Zamora, Laura Pallares, Christian Appel, Tobias Manuel Radice, Marcela Alejandra Castañeda Méndez, Paulo Gales, Ana C. Munita, José M. Villegas, María Virginia |
author_role |
author |
author2 |
De La Cadena, Elsa García Betancur, Juan Carlos Porras, Jessica Novoa Caicedo, Isabella Páez Zamora, Laura Pallares, Christian Appel, Tobias Manuel Radice, Marcela Alejandra Castañeda Méndez, Paulo Gales, Ana C. Munita, José M. Villegas, María Virginia |
author2_role |
author author author author author author author author author author author author |
dc.subject.none.fl_str_mv |
ANTIMICROBIAL RESISTANCE CEFTAZIDIME/AVIBACTAM ENTEROBACTERALES LATIN AMERICA PSEUDOMONAS AERUGINOSA |
topic |
ANTIMICROBIAL RESISTANCE CEFTAZIDIME/AVIBACTAM ENTEROBACTERALES LATIN AMERICA PSEUDOMONAS AERUGINOSA |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Ceftazidime-avibactam (CZA) is the combination of a third-generation cephalosporin and a new non-b-lactam b-lactamase inhibitor capable of inactivating class A, C, and some D b-lactamases. From a collection of 2,727 clinical isolates of Enterobacterales (n = 2,235) and P. aeruginosa (n = 492) that were collected between 2016 and 2017 from five Latin American countries, we investigated the molecular resistance mechanisms to CZA of 127 (18/2,235 [0.8%] Enterobacterales and 109/492 [22.1%] P. aeruginosa). First, by qPCR for the presence of genes encoding KPC, NDM, VIM, IMP, OXA-48-like, and SPM-1 carbapenemases, and second, by whole-genome sequencing (WGS). From the CZA-resistant isolates, MBL-encoding genes were detected in all 18 Enterobacterales and 42/109 P. aeruginosa isolates, explaining their resistant phenotype. Resistant isolates that yielded a negative qPCR result for any of the MBL encoding genes were subjected to WGS. The WGS analysis of the 67 remaining P. aeruginosa isolates showed mutations in genes previously associated with reduced susceptibility to CZA, such as those involved in the MexAB-OprM efflux pump and AmpC (PDC) hyperproduction, PoxB (blaOXA-50-like), FtsI (PBP3), DacB (PBP4), and OprD. The results presented here offer a snapshot of the molecular epidemiological landscape for CZA resistance before the introduction of this antibiotic into the Latin American market. Therefore, these results serve as a valuable comparison tool to trace the evolution of the resistance to CZA in this carbapenemase-endemic geographical region. Fil: Mojica, María Fernanda. Case Western Reserve University; Estados Unidos. Va Northeast Ohio Healthcare System; Estados Unidos. Universidad El Bosque; Fil: De La Cadena, Elsa. Universidad El Bosque; Fil: García Betancur, Juan Carlos. Universidad El Bosque; Fil: Porras, Jessica. Universidad El Bosque; Fil: Novoa Caicedo, Isabella. Universidad El Bosque; Fil: Páez Zamora, Laura. Universidad El Bosque; Fil: Pallares, Christian. Universidad El Bosque; Fil: Appel, Tobias Manuel. Universidad El Bosque; Fil: Radice, Marcela Alejandra. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina Fil: Castañeda Méndez, Paulo. Hospital San Ángel Inn Universidad; México. Fundacion Clinica Medica Sur; México Fil: Gales, Ana C.. Universidade Federal de Sao Paulo; Brasil Fil: Munita, José M.. Millennium Initiative for Collaborative Research on Bacterial Resistance; Chile Fil: Villegas, María Virginia. Universidad El Bosque; |
description |
Ceftazidime-avibactam (CZA) is the combination of a third-generation cephalosporin and a new non-b-lactam b-lactamase inhibitor capable of inactivating class A, C, and some D b-lactamases. From a collection of 2,727 clinical isolates of Enterobacterales (n = 2,235) and P. aeruginosa (n = 492) that were collected between 2016 and 2017 from five Latin American countries, we investigated the molecular resistance mechanisms to CZA of 127 (18/2,235 [0.8%] Enterobacterales and 109/492 [22.1%] P. aeruginosa). First, by qPCR for the presence of genes encoding KPC, NDM, VIM, IMP, OXA-48-like, and SPM-1 carbapenemases, and second, by whole-genome sequencing (WGS). From the CZA-resistant isolates, MBL-encoding genes were detected in all 18 Enterobacterales and 42/109 P. aeruginosa isolates, explaining their resistant phenotype. Resistant isolates that yielded a negative qPCR result for any of the MBL encoding genes were subjected to WGS. The WGS analysis of the 67 remaining P. aeruginosa isolates showed mutations in genes previously associated with reduced susceptibility to CZA, such as those involved in the MexAB-OprM efflux pump and AmpC (PDC) hyperproduction, PoxB (blaOXA-50-like), FtsI (PBP3), DacB (PBP4), and OprD. The results presented here offer a snapshot of the molecular epidemiological landscape for CZA resistance before the introduction of this antibiotic into the Latin American market. Therefore, these results serve as a valuable comparison tool to trace the evolution of the resistance to CZA in this carbapenemase-endemic geographical region. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-04 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/227554 Mojica, María Fernanda; De La Cadena, Elsa; García Betancur, Juan Carlos; Porras, Jessica; Novoa Caicedo, Isabella; et al.; Molecular Mechanisms of Resistance to Ceftazidime/Avibactam in Clinical Isolates of Enterobacterales and Pseudomonas aeruginosa in Latin American Hospitals; American Society for Microbiology; mSphere; 8; 2; 4-2023; 1-10 2379-5042 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/227554 |
identifier_str_mv |
Mojica, María Fernanda; De La Cadena, Elsa; García Betancur, Juan Carlos; Porras, Jessica; Novoa Caicedo, Isabella; et al.; Molecular Mechanisms of Resistance to Ceftazidime/Avibactam in Clinical Isolates of Enterobacterales and Pseudomonas aeruginosa in Latin American Hospitals; American Society for Microbiology; mSphere; 8; 2; 4-2023; 1-10 2379-5042 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1128/msphere.00651-22 info:eu-repo/semantics/altIdentifier/url/https://journals.asm.org/doi/10.1128/msphere.00651-22 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
American Society for Microbiology |
publisher.none.fl_str_mv |
American Society for Microbiology |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844614321617764352 |
score |
13.070432 |