Emergence of KPC-113 and KPC-114 variants in ceftazidime-avibactam-resistant Klebsiella pneumoniae belonging to high-risk clones ST11 and ST16 in South America

Autores
Vásquez Ponce, Felipe; Bispo, Jessica; Becerra, Johana; Fontana, Herrison; Pariona, Jesus G. M.; Esposito, Fernanda; Fuga, Bruna; Oliveira, Flavio A.; Brunetti, Florencia Lourdes; Power, Pablo; Gutkind, Gabriel Osvaldo; Schreiber, Angelica Zaninelli; Lincopan, Nilton
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Two novel variants of Klebsiella pneumoniae carbapenemase (KPC) associated with resistance to ceftazidime-avibactam (CZA) and designated as KPC-113 and KPC-114 by NCBI were identified in 2020, in clinical isolates of Klebsiella pneumoniae in Brazil. While K. pneumoniae of ST16 harbored the blaKPC-113 variant on an IncFII-IncFIB plasmid, K. pneumoniae of ST11 carried the blaKPC-114 variant on an IncN plasmid. Both isolates displayed resistance to broad-spectrum cephalosporins, β-lactam inhibitors, and ertapenem and doripenem, whereas K. pneumoniae producing KPC-114 showed susceptibility to imipenem and meropenem. Whole-genome sequencing and in silico analysis revealed that KPC-113 presented a Gly insertion between Ambler positions 264 and 265 (R264_A265insG), whereas KPC-114 displayed two amino acid insertions (Ser-Ser) between Ambler positions 181 and 182 (S181_P182insSS) in KPC-2, responsible for CZA resistance profiles. Our results confirm the emergence of novel KPC variants associated with resistance to CZA in international clones of K. pneumoniae circulating in South America. IMPORTANCE KPC-2 carbapenemases are endemic in Latin America. In this regard, in 2018, ceftazidime-avibactam (CZA) was authorized for clinical use in Brazil due to its significant activity against KPC-2 producers. In recent years, reports of resistance to CZA have increased in this country, limiting its clinical application. In this study, we report the emergence of two novel KPC-2 variants, named KPC-113 and KPC-114, associated with CZA resistance in Klebsiella pneumoniae strains belonging to high-risk clones ST11 and ST16. Our finding suggests that novel mutations in KPC-2 are increasing in South America, which is a critical issue deserving active surveillance.
Fil: Vásquez Ponce, Felipe. Universidade de Sao Paulo; Brasil. One Health Brazilian Resistance Project; Brasil
Fil: Bispo, Jessica. Universidade de Sao Paulo; Brasil. One Health Brazilian Resistance Project; Brasil
Fil: Becerra, Johana. Universidade de Sao Paulo; Brasil. One Health Brazilian Resistance Project; Brasil
Fil: Fontana, Herrison. One Health Brazilian Resistance Project; Brasil. Universidade de Sao Paulo; Brasil
Fil: Pariona, Jesus G. M.. One Health Brazilian Resistance Project; Brasil. Universidade de Sao Paulo; Brasil
Fil: Esposito, Fernanda. One Health Brazilian Resistance Project; Brasil. Universidade de Sao Paulo; Brasil
Fil: Fuga, Bruna. One Health Brazilian Resistance Project; Brasil. Universidade de Sao Paulo; Brasil
Fil: Oliveira, Flavio A.. Universidade Estadual de Campinas; Brasil
Fil: Brunetti, Florencia Lourdes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones en Bacteriología y Virología Molecular; Argentina
Fil: Power, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones en Bacteriología y Virología Molecular; Argentina
Fil: Gutkind, Gabriel Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones en Bacteriología y Virología Molecular; Argentina
Fil: Schreiber, Angelica Zaninelli. Universidade Estadual de Campinas; Brasil
Fil: Lincopan, Nilton. Universidade de Sao Paulo; Brasil. One Health Brazilian Resistance Project; Brasil
Materia
ANTIMICROBIAL RESISTANCE
CEFTAZIDIME-AVIBACTAM
ENTEROBACTERALES
GENOMIC SURVEILLANCE
INTERNATIONAL CLONES
KPC VARIANTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/225095
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/225095
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Emergence of KPC-113 and KPC-114 variants in ceftazidime-avibactam-resistant Klebsiella pneumoniae belonging to high-risk clones ST11 and ST16 in South AmericaVásquez Ponce, FelipeBispo, JessicaBecerra, JohanaFontana, HerrisonPariona, Jesus G. M.Esposito, FernandaFuga, BrunaOliveira, Flavio A.Brunetti, Florencia LourdesPower, PabloGutkind, Gabriel OsvaldoSchreiber, Angelica ZaninelliLincopan, NiltonANTIMICROBIAL RESISTANCECEFTAZIDIME-AVIBACTAMENTEROBACTERALESGENOMIC SURVEILLANCEINTERNATIONAL CLONESKPC VARIANTShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Two novel variants of Klebsiella pneumoniae carbapenemase (KPC) associated with resistance to ceftazidime-avibactam (CZA) and designated as KPC-113 and KPC-114 by NCBI were identified in 2020, in clinical isolates of Klebsiella pneumoniae in Brazil. While K. pneumoniae of ST16 harbored the blaKPC-113 variant on an IncFII-IncFIB plasmid, K. pneumoniae of ST11 carried the blaKPC-114 variant on an IncN plasmid. Both isolates displayed resistance to broad-spectrum cephalosporins, β-lactam inhibitors, and ertapenem and doripenem, whereas K. pneumoniae producing KPC-114 showed susceptibility to imipenem and meropenem. Whole-genome sequencing and in silico analysis revealed that KPC-113 presented a Gly insertion between Ambler positions 264 and 265 (R264_A265insG), whereas KPC-114 displayed two amino acid insertions (Ser-Ser) between Ambler positions 181 and 182 (S181_P182insSS) in KPC-2, responsible for CZA resistance profiles. Our results confirm the emergence of novel KPC variants associated with resistance to CZA in international clones of K. pneumoniae circulating in South America. IMPORTANCE KPC-2 carbapenemases are endemic in Latin America. In this regard, in 2018, ceftazidime-avibactam (CZA) was authorized for clinical use in Brazil due to its significant activity against KPC-2 producers. In recent years, reports of resistance to CZA have increased in this country, limiting its clinical application. In this study, we report the emergence of two novel KPC-2 variants, named KPC-113 and KPC-114, associated with CZA resistance in Klebsiella pneumoniae strains belonging to high-risk clones ST11 and ST16. Our finding suggests that novel mutations in KPC-2 are increasing in South America, which is a critical issue deserving active surveillance.Fil: Vásquez Ponce, Felipe. Universidade de Sao Paulo; Brasil. One Health Brazilian Resistance Project; BrasilFil: Bispo, Jessica. Universidade de Sao Paulo; Brasil. One Health Brazilian Resistance Project; BrasilFil: Becerra, Johana. Universidade de Sao Paulo; Brasil. One Health Brazilian Resistance Project; BrasilFil: Fontana, Herrison. One Health Brazilian Resistance Project; Brasil. Universidade de Sao Paulo; BrasilFil: Pariona, Jesus G. M.. One Health Brazilian Resistance Project; Brasil. Universidade de Sao Paulo; BrasilFil: Esposito, Fernanda. One Health Brazilian Resistance Project; Brasil. Universidade de Sao Paulo; BrasilFil: Fuga, Bruna. One Health Brazilian Resistance Project; Brasil. Universidade de Sao Paulo; BrasilFil: Oliveira, Flavio A.. Universidade Estadual de Campinas; BrasilFil: Brunetti, Florencia Lourdes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones en Bacteriología y Virología Molecular; ArgentinaFil: Power, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones en Bacteriología y Virología Molecular; ArgentinaFil: Gutkind, Gabriel Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones en Bacteriología y Virología Molecular; ArgentinaFil: Schreiber, Angelica Zaninelli. Universidade Estadual de Campinas; BrasilFil: Lincopan, Nilton. Universidade de Sao Paulo; Brasil. One Health Brazilian Resistance Project; BrasilAmerican Society for Microbiology2023-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/225095Vásquez Ponce, Felipe; Bispo, Jessica; Becerra, Johana; Fontana, Herrison; Pariona, Jesus G. M.; et al.; Emergence of KPC-113 and KPC-114 variants in ceftazidime-avibactam-resistant Klebsiella pneumoniae belonging to high-risk clones ST11 and ST16 in South America; American Society for Microbiology; Microbiology Spectrum; 11; 5; 10-2023; 1-92165-0497CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.asm.org/doi/10.1128/spectrum.00374-23info:eu-repo/semantics/altIdentifier/doi/10.1128/spectrum.00374-23info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:14:13Zoai:ri.conicet.gov.ar:11336/225095instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:14:13.351CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Emergence of KPC-113 and KPC-114 variants in ceftazidime-avibactam-resistant Klebsiella pneumoniae belonging to high-risk clones ST11 and ST16 in South America
title Emergence of KPC-113 and KPC-114 variants in ceftazidime-avibactam-resistant Klebsiella pneumoniae belonging to high-risk clones ST11 and ST16 in South America
spellingShingle Emergence of KPC-113 and KPC-114 variants in ceftazidime-avibactam-resistant Klebsiella pneumoniae belonging to high-risk clones ST11 and ST16 in South America
Vásquez Ponce, Felipe
ANTIMICROBIAL RESISTANCE
CEFTAZIDIME-AVIBACTAM
ENTEROBACTERALES
GENOMIC SURVEILLANCE
INTERNATIONAL CLONES
KPC VARIANTS
title_short Emergence of KPC-113 and KPC-114 variants in ceftazidime-avibactam-resistant Klebsiella pneumoniae belonging to high-risk clones ST11 and ST16 in South America
title_full Emergence of KPC-113 and KPC-114 variants in ceftazidime-avibactam-resistant Klebsiella pneumoniae belonging to high-risk clones ST11 and ST16 in South America
title_fullStr Emergence of KPC-113 and KPC-114 variants in ceftazidime-avibactam-resistant Klebsiella pneumoniae belonging to high-risk clones ST11 and ST16 in South America
title_full_unstemmed Emergence of KPC-113 and KPC-114 variants in ceftazidime-avibactam-resistant Klebsiella pneumoniae belonging to high-risk clones ST11 and ST16 in South America
title_sort Emergence of KPC-113 and KPC-114 variants in ceftazidime-avibactam-resistant Klebsiella pneumoniae belonging to high-risk clones ST11 and ST16 in South America
dc.creator.none.fl_str_mv Vásquez Ponce, Felipe
Bispo, Jessica
Becerra, Johana
Fontana, Herrison
Pariona, Jesus G. M.
Esposito, Fernanda
Fuga, Bruna
Oliveira, Flavio A.
Brunetti, Florencia Lourdes
Power, Pablo
Gutkind, Gabriel Osvaldo
Schreiber, Angelica Zaninelli
Lincopan, Nilton
author Vásquez Ponce, Felipe
author_facet Vásquez Ponce, Felipe
Bispo, Jessica
Becerra, Johana
Fontana, Herrison
Pariona, Jesus G. M.
Esposito, Fernanda
Fuga, Bruna
Oliveira, Flavio A.
Brunetti, Florencia Lourdes
Power, Pablo
Gutkind, Gabriel Osvaldo
Schreiber, Angelica Zaninelli
Lincopan, Nilton
author_role author
author2 Bispo, Jessica
Becerra, Johana
Fontana, Herrison
Pariona, Jesus G. M.
Esposito, Fernanda
Fuga, Bruna
Oliveira, Flavio A.
Brunetti, Florencia Lourdes
Power, Pablo
Gutkind, Gabriel Osvaldo
Schreiber, Angelica Zaninelli
Lincopan, Nilton
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ANTIMICROBIAL RESISTANCE
CEFTAZIDIME-AVIBACTAM
ENTEROBACTERALES
GENOMIC SURVEILLANCE
INTERNATIONAL CLONES
KPC VARIANTS
topic ANTIMICROBIAL RESISTANCE
CEFTAZIDIME-AVIBACTAM
ENTEROBACTERALES
GENOMIC SURVEILLANCE
INTERNATIONAL CLONES
KPC VARIANTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Two novel variants of Klebsiella pneumoniae carbapenemase (KPC) associated with resistance to ceftazidime-avibactam (CZA) and designated as KPC-113 and KPC-114 by NCBI were identified in 2020, in clinical isolates of Klebsiella pneumoniae in Brazil. While K. pneumoniae of ST16 harbored the blaKPC-113 variant on an IncFII-IncFIB plasmid, K. pneumoniae of ST11 carried the blaKPC-114 variant on an IncN plasmid. Both isolates displayed resistance to broad-spectrum cephalosporins, β-lactam inhibitors, and ertapenem and doripenem, whereas K. pneumoniae producing KPC-114 showed susceptibility to imipenem and meropenem. Whole-genome sequencing and in silico analysis revealed that KPC-113 presented a Gly insertion between Ambler positions 264 and 265 (R264_A265insG), whereas KPC-114 displayed two amino acid insertions (Ser-Ser) between Ambler positions 181 and 182 (S181_P182insSS) in KPC-2, responsible for CZA resistance profiles. Our results confirm the emergence of novel KPC variants associated with resistance to CZA in international clones of K. pneumoniae circulating in South America. IMPORTANCE KPC-2 carbapenemases are endemic in Latin America. In this regard, in 2018, ceftazidime-avibactam (CZA) was authorized for clinical use in Brazil due to its significant activity against KPC-2 producers. In recent years, reports of resistance to CZA have increased in this country, limiting its clinical application. In this study, we report the emergence of two novel KPC-2 variants, named KPC-113 and KPC-114, associated with CZA resistance in Klebsiella pneumoniae strains belonging to high-risk clones ST11 and ST16. Our finding suggests that novel mutations in KPC-2 are increasing in South America, which is a critical issue deserving active surveillance.
Fil: Vásquez Ponce, Felipe. Universidade de Sao Paulo; Brasil. One Health Brazilian Resistance Project; Brasil
Fil: Bispo, Jessica. Universidade de Sao Paulo; Brasil. One Health Brazilian Resistance Project; Brasil
Fil: Becerra, Johana. Universidade de Sao Paulo; Brasil. One Health Brazilian Resistance Project; Brasil
Fil: Fontana, Herrison. One Health Brazilian Resistance Project; Brasil. Universidade de Sao Paulo; Brasil
Fil: Pariona, Jesus G. M.. One Health Brazilian Resistance Project; Brasil. Universidade de Sao Paulo; Brasil
Fil: Esposito, Fernanda. One Health Brazilian Resistance Project; Brasil. Universidade de Sao Paulo; Brasil
Fil: Fuga, Bruna. One Health Brazilian Resistance Project; Brasil. Universidade de Sao Paulo; Brasil
Fil: Oliveira, Flavio A.. Universidade Estadual de Campinas; Brasil
Fil: Brunetti, Florencia Lourdes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones en Bacteriología y Virología Molecular; Argentina
Fil: Power, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones en Bacteriología y Virología Molecular; Argentina
Fil: Gutkind, Gabriel Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones en Bacteriología y Virología Molecular; Argentina
Fil: Schreiber, Angelica Zaninelli. Universidade Estadual de Campinas; Brasil
Fil: Lincopan, Nilton. Universidade de Sao Paulo; Brasil. One Health Brazilian Resistance Project; Brasil
description Two novel variants of Klebsiella pneumoniae carbapenemase (KPC) associated with resistance to ceftazidime-avibactam (CZA) and designated as KPC-113 and KPC-114 by NCBI were identified in 2020, in clinical isolates of Klebsiella pneumoniae in Brazil. While K. pneumoniae of ST16 harbored the blaKPC-113 variant on an IncFII-IncFIB plasmid, K. pneumoniae of ST11 carried the blaKPC-114 variant on an IncN plasmid. Both isolates displayed resistance to broad-spectrum cephalosporins, β-lactam inhibitors, and ertapenem and doripenem, whereas K. pneumoniae producing KPC-114 showed susceptibility to imipenem and meropenem. Whole-genome sequencing and in silico analysis revealed that KPC-113 presented a Gly insertion between Ambler positions 264 and 265 (R264_A265insG), whereas KPC-114 displayed two amino acid insertions (Ser-Ser) between Ambler positions 181 and 182 (S181_P182insSS) in KPC-2, responsible for CZA resistance profiles. Our results confirm the emergence of novel KPC variants associated with resistance to CZA in international clones of K. pneumoniae circulating in South America. IMPORTANCE KPC-2 carbapenemases are endemic in Latin America. In this regard, in 2018, ceftazidime-avibactam (CZA) was authorized for clinical use in Brazil due to its significant activity against KPC-2 producers. In recent years, reports of resistance to CZA have increased in this country, limiting its clinical application. In this study, we report the emergence of two novel KPC-2 variants, named KPC-113 and KPC-114, associated with CZA resistance in Klebsiella pneumoniae strains belonging to high-risk clones ST11 and ST16. Our finding suggests that novel mutations in KPC-2 are increasing in South America, which is a critical issue deserving active surveillance.
publishDate 2023
dc.date.none.fl_str_mv 2023-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/225095
Vásquez Ponce, Felipe; Bispo, Jessica; Becerra, Johana; Fontana, Herrison; Pariona, Jesus G. M.; et al.; Emergence of KPC-113 and KPC-114 variants in ceftazidime-avibactam-resistant Klebsiella pneumoniae belonging to high-risk clones ST11 and ST16 in South America; American Society for Microbiology; Microbiology Spectrum; 11; 5; 10-2023; 1-9
2165-0497
CONICET Digital
CONICET
url http://hdl.handle.net/11336/225095
identifier_str_mv Vásquez Ponce, Felipe; Bispo, Jessica; Becerra, Johana; Fontana, Herrison; Pariona, Jesus G. M.; et al.; Emergence of KPC-113 and KPC-114 variants in ceftazidime-avibactam-resistant Klebsiella pneumoniae belonging to high-risk clones ST11 and ST16 in South America; American Society for Microbiology; Microbiology Spectrum; 11; 5; 10-2023; 1-9
2165-0497
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.asm.org/doi/10.1128/spectrum.00374-23
info:eu-repo/semantics/altIdentifier/doi/10.1128/spectrum.00374-23
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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