Emergence of KPC-113 and KPC-114 variants in ceftazidime-avibactam-resistant Klebsiella pneumoniae belonging to high-risk clones ST11 and ST16 in South America
- Autores
- Vásquez Ponce, Felipe; Bispo, Jessica; Becerra, Johana; Fontana, Herrison; Pariona, Jesus G. M.; Esposito, Fernanda; Fuga, Bruna; Oliveira, Flavio A.; Brunetti, Florencia Lourdes; Power, Pablo; Gutkind, Gabriel Osvaldo; Schreiber, Angelica Zaninelli; Lincopan, Nilton
- Año de publicación
- 2023
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Two novel variants of Klebsiella pneumoniae carbapenemase (KPC) associated with resistance to ceftazidime-avibactam (CZA) and designated as KPC-113 and KPC-114 by NCBI were identified in 2020, in clinical isolates of Klebsiella pneumoniae in Brazil. While K. pneumoniae of ST16 harbored the blaKPC-113 variant on an IncFII-IncFIB plasmid, K. pneumoniae of ST11 carried the blaKPC-114 variant on an IncN plasmid. Both isolates displayed resistance to broad-spectrum cephalosporins, β-lactam inhibitors, and ertapenem and doripenem, whereas K. pneumoniae producing KPC-114 showed susceptibility to imipenem and meropenem. Whole-genome sequencing and in silico analysis revealed that KPC-113 presented a Gly insertion between Ambler positions 264 and 265 (R264_A265insG), whereas KPC-114 displayed two amino acid insertions (Ser-Ser) between Ambler positions 181 and 182 (S181_P182insSS) in KPC-2, responsible for CZA resistance profiles. Our results confirm the emergence of novel KPC variants associated with resistance to CZA in international clones of K. pneumoniae circulating in South America. IMPORTANCE KPC-2 carbapenemases are endemic in Latin America. In this regard, in 2018, ceftazidime-avibactam (CZA) was authorized for clinical use in Brazil due to its significant activity against KPC-2 producers. In recent years, reports of resistance to CZA have increased in this country, limiting its clinical application. In this study, we report the emergence of two novel KPC-2 variants, named KPC-113 and KPC-114, associated with CZA resistance in Klebsiella pneumoniae strains belonging to high-risk clones ST11 and ST16. Our finding suggests that novel mutations in KPC-2 are increasing in South America, which is a critical issue deserving active surveillance.
Fil: Vásquez Ponce, Felipe. Universidade de Sao Paulo; Brasil. One Health Brazilian Resistance Project; Brasil
Fil: Bispo, Jessica. Universidade de Sao Paulo; Brasil. One Health Brazilian Resistance Project; Brasil
Fil: Becerra, Johana. Universidade de Sao Paulo; Brasil. One Health Brazilian Resistance Project; Brasil
Fil: Fontana, Herrison. One Health Brazilian Resistance Project; Brasil. Universidade de Sao Paulo; Brasil
Fil: Pariona, Jesus G. M.. One Health Brazilian Resistance Project; Brasil. Universidade de Sao Paulo; Brasil
Fil: Esposito, Fernanda. One Health Brazilian Resistance Project; Brasil. Universidade de Sao Paulo; Brasil
Fil: Fuga, Bruna. One Health Brazilian Resistance Project; Brasil. Universidade de Sao Paulo; Brasil
Fil: Oliveira, Flavio A.. Universidade Estadual de Campinas; Brasil
Fil: Brunetti, Florencia Lourdes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones en Bacteriología y Virología Molecular; Argentina
Fil: Power, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones en Bacteriología y Virología Molecular; Argentina
Fil: Gutkind, Gabriel Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones en Bacteriología y Virología Molecular; Argentina
Fil: Schreiber, Angelica Zaninelli. Universidade Estadual de Campinas; Brasil
Fil: Lincopan, Nilton. Universidade de Sao Paulo; Brasil. One Health Brazilian Resistance Project; Brasil - Materia
-
ANTIMICROBIAL RESISTANCE
CEFTAZIDIME-AVIBACTAM
ENTEROBACTERALES
GENOMIC SURVEILLANCE
INTERNATIONAL CLONES
KPC VARIANTS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/225095
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spelling |
Emergence of KPC-113 and KPC-114 variants in ceftazidime-avibactam-resistant Klebsiella pneumoniae belonging to high-risk clones ST11 and ST16 in South AmericaVásquez Ponce, FelipeBispo, JessicaBecerra, JohanaFontana, HerrisonPariona, Jesus G. M.Esposito, FernandaFuga, BrunaOliveira, Flavio A.Brunetti, Florencia LourdesPower, PabloGutkind, Gabriel OsvaldoSchreiber, Angelica ZaninelliLincopan, NiltonANTIMICROBIAL RESISTANCECEFTAZIDIME-AVIBACTAMENTEROBACTERALESGENOMIC SURVEILLANCEINTERNATIONAL CLONESKPC VARIANTShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Two novel variants of Klebsiella pneumoniae carbapenemase (KPC) associated with resistance to ceftazidime-avibactam (CZA) and designated as KPC-113 and KPC-114 by NCBI were identified in 2020, in clinical isolates of Klebsiella pneumoniae in Brazil. While K. pneumoniae of ST16 harbored the blaKPC-113 variant on an IncFII-IncFIB plasmid, K. pneumoniae of ST11 carried the blaKPC-114 variant on an IncN plasmid. Both isolates displayed resistance to broad-spectrum cephalosporins, β-lactam inhibitors, and ertapenem and doripenem, whereas K. pneumoniae producing KPC-114 showed susceptibility to imipenem and meropenem. Whole-genome sequencing and in silico analysis revealed that KPC-113 presented a Gly insertion between Ambler positions 264 and 265 (R264_A265insG), whereas KPC-114 displayed two amino acid insertions (Ser-Ser) between Ambler positions 181 and 182 (S181_P182insSS) in KPC-2, responsible for CZA resistance profiles. Our results confirm the emergence of novel KPC variants associated with resistance to CZA in international clones of K. pneumoniae circulating in South America. IMPORTANCE KPC-2 carbapenemases are endemic in Latin America. In this regard, in 2018, ceftazidime-avibactam (CZA) was authorized for clinical use in Brazil due to its significant activity against KPC-2 producers. In recent years, reports of resistance to CZA have increased in this country, limiting its clinical application. In this study, we report the emergence of two novel KPC-2 variants, named KPC-113 and KPC-114, associated with CZA resistance in Klebsiella pneumoniae strains belonging to high-risk clones ST11 and ST16. Our finding suggests that novel mutations in KPC-2 are increasing in South America, which is a critical issue deserving active surveillance.Fil: Vásquez Ponce, Felipe. Universidade de Sao Paulo; Brasil. One Health Brazilian Resistance Project; BrasilFil: Bispo, Jessica. Universidade de Sao Paulo; Brasil. One Health Brazilian Resistance Project; BrasilFil: Becerra, Johana. Universidade de Sao Paulo; Brasil. One Health Brazilian Resistance Project; BrasilFil: Fontana, Herrison. One Health Brazilian Resistance Project; Brasil. Universidade de Sao Paulo; BrasilFil: Pariona, Jesus G. M.. One Health Brazilian Resistance Project; Brasil. Universidade de Sao Paulo; BrasilFil: Esposito, Fernanda. One Health Brazilian Resistance Project; Brasil. Universidade de Sao Paulo; BrasilFil: Fuga, Bruna. One Health Brazilian Resistance Project; Brasil. Universidade de Sao Paulo; BrasilFil: Oliveira, Flavio A.. Universidade Estadual de Campinas; BrasilFil: Brunetti, Florencia Lourdes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones en Bacteriología y Virología Molecular; ArgentinaFil: Power, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones en Bacteriología y Virología Molecular; ArgentinaFil: Gutkind, Gabriel Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones en Bacteriología y Virología Molecular; ArgentinaFil: Schreiber, Angelica Zaninelli. Universidade Estadual de Campinas; BrasilFil: Lincopan, Nilton. Universidade de Sao Paulo; Brasil. One Health Brazilian Resistance Project; BrasilAmerican Society for Microbiology2023-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/225095Vásquez Ponce, Felipe; Bispo, Jessica; Becerra, Johana; Fontana, Herrison; Pariona, Jesus G. M.; et al.; Emergence of KPC-113 and KPC-114 variants in ceftazidime-avibactam-resistant Klebsiella pneumoniae belonging to high-risk clones ST11 and ST16 in South America; American Society for Microbiology; Microbiology Spectrum; 11; 5; 10-2023; 1-92165-0497CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.asm.org/doi/10.1128/spectrum.00374-23info:eu-repo/semantics/altIdentifier/doi/10.1128/spectrum.00374-23info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:45:20Zoai:ri.conicet.gov.ar:11336/225095instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:45:20.881CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Emergence of KPC-113 and KPC-114 variants in ceftazidime-avibactam-resistant Klebsiella pneumoniae belonging to high-risk clones ST11 and ST16 in South America |
title |
Emergence of KPC-113 and KPC-114 variants in ceftazidime-avibactam-resistant Klebsiella pneumoniae belonging to high-risk clones ST11 and ST16 in South America |
spellingShingle |
Emergence of KPC-113 and KPC-114 variants in ceftazidime-avibactam-resistant Klebsiella pneumoniae belonging to high-risk clones ST11 and ST16 in South America Vásquez Ponce, Felipe ANTIMICROBIAL RESISTANCE CEFTAZIDIME-AVIBACTAM ENTEROBACTERALES GENOMIC SURVEILLANCE INTERNATIONAL CLONES KPC VARIANTS |
title_short |
Emergence of KPC-113 and KPC-114 variants in ceftazidime-avibactam-resistant Klebsiella pneumoniae belonging to high-risk clones ST11 and ST16 in South America |
title_full |
Emergence of KPC-113 and KPC-114 variants in ceftazidime-avibactam-resistant Klebsiella pneumoniae belonging to high-risk clones ST11 and ST16 in South America |
title_fullStr |
Emergence of KPC-113 and KPC-114 variants in ceftazidime-avibactam-resistant Klebsiella pneumoniae belonging to high-risk clones ST11 and ST16 in South America |
title_full_unstemmed |
Emergence of KPC-113 and KPC-114 variants in ceftazidime-avibactam-resistant Klebsiella pneumoniae belonging to high-risk clones ST11 and ST16 in South America |
title_sort |
Emergence of KPC-113 and KPC-114 variants in ceftazidime-avibactam-resistant Klebsiella pneumoniae belonging to high-risk clones ST11 and ST16 in South America |
dc.creator.none.fl_str_mv |
Vásquez Ponce, Felipe Bispo, Jessica Becerra, Johana Fontana, Herrison Pariona, Jesus G. M. Esposito, Fernanda Fuga, Bruna Oliveira, Flavio A. Brunetti, Florencia Lourdes Power, Pablo Gutkind, Gabriel Osvaldo Schreiber, Angelica Zaninelli Lincopan, Nilton |
author |
Vásquez Ponce, Felipe |
author_facet |
Vásquez Ponce, Felipe Bispo, Jessica Becerra, Johana Fontana, Herrison Pariona, Jesus G. M. Esposito, Fernanda Fuga, Bruna Oliveira, Flavio A. Brunetti, Florencia Lourdes Power, Pablo Gutkind, Gabriel Osvaldo Schreiber, Angelica Zaninelli Lincopan, Nilton |
author_role |
author |
author2 |
Bispo, Jessica Becerra, Johana Fontana, Herrison Pariona, Jesus G. M. Esposito, Fernanda Fuga, Bruna Oliveira, Flavio A. Brunetti, Florencia Lourdes Power, Pablo Gutkind, Gabriel Osvaldo Schreiber, Angelica Zaninelli Lincopan, Nilton |
author2_role |
author author author author author author author author author author author author |
dc.subject.none.fl_str_mv |
ANTIMICROBIAL RESISTANCE CEFTAZIDIME-AVIBACTAM ENTEROBACTERALES GENOMIC SURVEILLANCE INTERNATIONAL CLONES KPC VARIANTS |
topic |
ANTIMICROBIAL RESISTANCE CEFTAZIDIME-AVIBACTAM ENTEROBACTERALES GENOMIC SURVEILLANCE INTERNATIONAL CLONES KPC VARIANTS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Two novel variants of Klebsiella pneumoniae carbapenemase (KPC) associated with resistance to ceftazidime-avibactam (CZA) and designated as KPC-113 and KPC-114 by NCBI were identified in 2020, in clinical isolates of Klebsiella pneumoniae in Brazil. While K. pneumoniae of ST16 harbored the blaKPC-113 variant on an IncFII-IncFIB plasmid, K. pneumoniae of ST11 carried the blaKPC-114 variant on an IncN plasmid. Both isolates displayed resistance to broad-spectrum cephalosporins, β-lactam inhibitors, and ertapenem and doripenem, whereas K. pneumoniae producing KPC-114 showed susceptibility to imipenem and meropenem. Whole-genome sequencing and in silico analysis revealed that KPC-113 presented a Gly insertion between Ambler positions 264 and 265 (R264_A265insG), whereas KPC-114 displayed two amino acid insertions (Ser-Ser) between Ambler positions 181 and 182 (S181_P182insSS) in KPC-2, responsible for CZA resistance profiles. Our results confirm the emergence of novel KPC variants associated with resistance to CZA in international clones of K. pneumoniae circulating in South America. IMPORTANCE KPC-2 carbapenemases are endemic in Latin America. In this regard, in 2018, ceftazidime-avibactam (CZA) was authorized for clinical use in Brazil due to its significant activity against KPC-2 producers. In recent years, reports of resistance to CZA have increased in this country, limiting its clinical application. In this study, we report the emergence of two novel KPC-2 variants, named KPC-113 and KPC-114, associated with CZA resistance in Klebsiella pneumoniae strains belonging to high-risk clones ST11 and ST16. Our finding suggests that novel mutations in KPC-2 are increasing in South America, which is a critical issue deserving active surveillance. Fil: Vásquez Ponce, Felipe. Universidade de Sao Paulo; Brasil. One Health Brazilian Resistance Project; Brasil Fil: Bispo, Jessica. Universidade de Sao Paulo; Brasil. One Health Brazilian Resistance Project; Brasil Fil: Becerra, Johana. Universidade de Sao Paulo; Brasil. One Health Brazilian Resistance Project; Brasil Fil: Fontana, Herrison. One Health Brazilian Resistance Project; Brasil. Universidade de Sao Paulo; Brasil Fil: Pariona, Jesus G. M.. One Health Brazilian Resistance Project; Brasil. Universidade de Sao Paulo; Brasil Fil: Esposito, Fernanda. One Health Brazilian Resistance Project; Brasil. Universidade de Sao Paulo; Brasil Fil: Fuga, Bruna. One Health Brazilian Resistance Project; Brasil. Universidade de Sao Paulo; Brasil Fil: Oliveira, Flavio A.. Universidade Estadual de Campinas; Brasil Fil: Brunetti, Florencia Lourdes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones en Bacteriología y Virología Molecular; Argentina Fil: Power, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones en Bacteriología y Virología Molecular; Argentina Fil: Gutkind, Gabriel Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones en Bacteriología y Virología Molecular; Argentina Fil: Schreiber, Angelica Zaninelli. Universidade Estadual de Campinas; Brasil Fil: Lincopan, Nilton. Universidade de Sao Paulo; Brasil. One Health Brazilian Resistance Project; Brasil |
description |
Two novel variants of Klebsiella pneumoniae carbapenemase (KPC) associated with resistance to ceftazidime-avibactam (CZA) and designated as KPC-113 and KPC-114 by NCBI were identified in 2020, in clinical isolates of Klebsiella pneumoniae in Brazil. While K. pneumoniae of ST16 harbored the blaKPC-113 variant on an IncFII-IncFIB plasmid, K. pneumoniae of ST11 carried the blaKPC-114 variant on an IncN plasmid. Both isolates displayed resistance to broad-spectrum cephalosporins, β-lactam inhibitors, and ertapenem and doripenem, whereas K. pneumoniae producing KPC-114 showed susceptibility to imipenem and meropenem. Whole-genome sequencing and in silico analysis revealed that KPC-113 presented a Gly insertion between Ambler positions 264 and 265 (R264_A265insG), whereas KPC-114 displayed two amino acid insertions (Ser-Ser) between Ambler positions 181 and 182 (S181_P182insSS) in KPC-2, responsible for CZA resistance profiles. Our results confirm the emergence of novel KPC variants associated with resistance to CZA in international clones of K. pneumoniae circulating in South America. IMPORTANCE KPC-2 carbapenemases are endemic in Latin America. In this regard, in 2018, ceftazidime-avibactam (CZA) was authorized for clinical use in Brazil due to its significant activity against KPC-2 producers. In recent years, reports of resistance to CZA have increased in this country, limiting its clinical application. In this study, we report the emergence of two novel KPC-2 variants, named KPC-113 and KPC-114, associated with CZA resistance in Klebsiella pneumoniae strains belonging to high-risk clones ST11 and ST16. Our finding suggests that novel mutations in KPC-2 are increasing in South America, which is a critical issue deserving active surveillance. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/225095 Vásquez Ponce, Felipe; Bispo, Jessica; Becerra, Johana; Fontana, Herrison; Pariona, Jesus G. M.; et al.; Emergence of KPC-113 and KPC-114 variants in ceftazidime-avibactam-resistant Klebsiella pneumoniae belonging to high-risk clones ST11 and ST16 in South America; American Society for Microbiology; Microbiology Spectrum; 11; 5; 10-2023; 1-9 2165-0497 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/225095 |
identifier_str_mv |
Vásquez Ponce, Felipe; Bispo, Jessica; Becerra, Johana; Fontana, Herrison; Pariona, Jesus G. M.; et al.; Emergence of KPC-113 and KPC-114 variants in ceftazidime-avibactam-resistant Klebsiella pneumoniae belonging to high-risk clones ST11 and ST16 in South America; American Society for Microbiology; Microbiology Spectrum; 11; 5; 10-2023; 1-9 2165-0497 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://journals.asm.org/doi/10.1128/spectrum.00374-23 info:eu-repo/semantics/altIdentifier/doi/10.1128/spectrum.00374-23 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
American Society for Microbiology |
publisher.none.fl_str_mv |
American Society for Microbiology |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |