Wastewater surveillance of enteric viruses in eastern Argentina: High rates of detection and first report of NoV GI.5 and GII.20

Autores
Frydman, Camila Ayelen; Miño, S.; Iglesias, N. G.; Carballeda, J. M.; Simari, Milagros Belén; Pisano, María Belén; Dus Santos, María José; Mozgovoj, Marina Valeria
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Individuals infected with enteric viruses excrete them in their feces for an extended period, whether symptomatic or asymptomatic. This characteristic, combined with the capability of these viruses to persist in the environment, forms the core of our research. The objective of our study was to investigate the presence of viruses associated with diarrhea and hepatitis: Hepatitis A virus (HAV), Hepatitis E virus (HEV), Norovirus GI (NoV GI), Norovirus GII (NoV GII), and Rotavirus (RV) by RT-real time PCR, in untreated wastewater samples (n=100) collected in the period July 2020 to August 2021 from two low-income neighborhoods in the district of La Plata, Buenos Aires, Argentina. Globally, the percentage of positive samples was 25 % for HEV, 27 % for RV, 14 % for NoV GII, 1 % for NoV GI, and no detectable samples were found for HAV. HEV, RV, and NoV GII were detected in most of the studied months, with the highest detection rates of RV and NoV GII during the winter season. Regarding RV positive samples, the gene encoding the VP8* protein of three samples was sequenced and phylogenetic analysis revealed that the detected strains belonged to genotypes P[8] and P[3]. Additionally, four NoV strains were also genetically characterized by amplifying a fragment corresponding to the ORF-1/ORF-2 junction region. The identified strains were NoV GI.5, NoV GII.4, NoV GII.17 and NoV GII.20. Our results provide relevant information and serve as scientific evidence of the importance of considering wastewater analysis as a feasible strategy to determine the circulation of enteric viruses in the population, with the further benefit of predicting emerging strains. Moreover, this study represents the first report of the circulation of NoV GII.20 and GI.5 genotypes in our country.
Fil: Frydman, Camila Ayelen. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Agroindustria. Instituto de Tecnología de Alimentos. Instituto de Ciencia y Tecnología de Sistemas Alimentarios Sustentables. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología de Sistemas Alimentarios Sustentables; Argentina. Universidad Nacional de Hurlingham.; Argentina
Fil: Miño, S.. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Misiones. Estacion Experimental Agropecuaria Cerro Azul.; Argentina
Fil: Iglesias, N. G.. Universidad Nacional de Hurlingham.; Argentina
Fil: Carballeda, J. M.. Universidad Nacional de Hurlingham.; Argentina
Fil: Simari, Milagros Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; Argentina
Fil: Pisano, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina
Fil: Dus Santos, María José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología e Innovaciones Tecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Virología e Innovaciones Tecnológicas; Argentina. Universidad Nacional de Hurlingham.; Argentina
Fil: Mozgovoj, Marina Valeria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Agroindustria. Instituto de Tecnología de Alimentos. Instituto de Ciencia y Tecnología de Sistemas Alimentarios Sustentables. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología de Sistemas Alimentarios Sustentables; Argentina. Universidad Nacional de Hurlingham.; Argentina
Materia
SEWAGE
ENTERICVIRUSES
PHYLOGENETIC ANALYSIS
NOROVIRUS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/231049
Acceder
id CONICETDig_ac1745146c9019f3692a606e0192864e
oai_identifier_str oai:ri.conicet.gov.ar:11336/231049
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Wastewater surveillance of enteric viruses in eastern Argentina: High rates of detection and first report of NoV GI.5 and GII.20Frydman, Camila AyelenMiño, S.Iglesias, N. G.Carballeda, J. M.Simari, Milagros BelénPisano, María BelénDus Santos, María JoséMozgovoj, Marina ValeriaSEWAGEENTERICVIRUSESPHYLOGENETIC ANALYSISNOROVIRUShttps://purl.org/becyt/ford/1.7https://purl.org/becyt/ford/1Individuals infected with enteric viruses excrete them in their feces for an extended period, whether symptomatic or asymptomatic. This characteristic, combined with the capability of these viruses to persist in the environment, forms the core of our research. The objective of our study was to investigate the presence of viruses associated with diarrhea and hepatitis: Hepatitis A virus (HAV), Hepatitis E virus (HEV), Norovirus GI (NoV GI), Norovirus GII (NoV GII), and Rotavirus (RV) by RT-real time PCR, in untreated wastewater samples (n=100) collected in the period July 2020 to August 2021 from two low-income neighborhoods in the district of La Plata, Buenos Aires, Argentina. Globally, the percentage of positive samples was 25 % for HEV, 27 % for RV, 14 % for NoV GII, 1 % for NoV GI, and no detectable samples were found for HAV. HEV, RV, and NoV GII were detected in most of the studied months, with the highest detection rates of RV and NoV GII during the winter season. Regarding RV positive samples, the gene encoding the VP8* protein of three samples was sequenced and phylogenetic analysis revealed that the detected strains belonged to genotypes P[8] and P[3]. Additionally, four NoV strains were also genetically characterized by amplifying a fragment corresponding to the ORF-1/ORF-2 junction region. The identified strains were NoV GI.5, NoV GII.4, NoV GII.17 and NoV GII.20. Our results provide relevant information and serve as scientific evidence of the importance of considering wastewater analysis as a feasible strategy to determine the circulation of enteric viruses in the population, with the further benefit of predicting emerging strains. Moreover, this study represents the first report of the circulation of NoV GII.20 and GI.5 genotypes in our country.Fil: Frydman, Camila Ayelen. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Agroindustria. Instituto de Tecnología de Alimentos. Instituto de Ciencia y Tecnología de Sistemas Alimentarios Sustentables. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología de Sistemas Alimentarios Sustentables; Argentina. Universidad Nacional de Hurlingham.; ArgentinaFil: Miño, S.. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Misiones. Estacion Experimental Agropecuaria Cerro Azul.; ArgentinaFil: Iglesias, N. G.. Universidad Nacional de Hurlingham.; ArgentinaFil: Carballeda, J. M.. Universidad Nacional de Hurlingham.; ArgentinaFil: Simari, Milagros Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; ArgentinaFil: Pisano, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; ArgentinaFil: Dus Santos, María José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología e Innovaciones Tecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Virología e Innovaciones Tecnológicas; Argentina. Universidad Nacional de Hurlingham.; ArgentinaFil: Mozgovoj, Marina Valeria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Agroindustria. Instituto de Tecnología de Alimentos. Instituto de Ciencia y Tecnología de Sistemas Alimentarios Sustentables. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología de Sistemas Alimentarios Sustentables; Argentina. Universidad Nacional de Hurlingham.; ArgentinaElsevier2024-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/231049Frydman, Camila Ayelen; Miño, S.; Iglesias, N. G.; Carballeda, J. M.; Simari, Milagros Belén; et al.; Wastewater surveillance of enteric viruses in eastern Argentina: High rates of detection and first report of NoV GI.5 and GII.20; Elsevier; Environmental Advances; 15; 4-2024; 1-102666-7657CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S266676572400019Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.envadv.2024.100501info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:19Zoai:ri.conicet.gov.ar:11336/231049instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:19.608CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Wastewater surveillance of enteric viruses in eastern Argentina: High rates of detection and first report of NoV GI.5 and GII.20
title Wastewater surveillance of enteric viruses in eastern Argentina: High rates of detection and first report of NoV GI.5 and GII.20
spellingShingle Wastewater surveillance of enteric viruses in eastern Argentina: High rates of detection and first report of NoV GI.5 and GII.20
Frydman, Camila Ayelen
SEWAGE
ENTERICVIRUSES
PHYLOGENETIC ANALYSIS
NOROVIRUS
title_short Wastewater surveillance of enteric viruses in eastern Argentina: High rates of detection and first report of NoV GI.5 and GII.20
title_full Wastewater surveillance of enteric viruses in eastern Argentina: High rates of detection and first report of NoV GI.5 and GII.20
title_fullStr Wastewater surveillance of enteric viruses in eastern Argentina: High rates of detection and first report of NoV GI.5 and GII.20
title_full_unstemmed Wastewater surveillance of enteric viruses in eastern Argentina: High rates of detection and first report of NoV GI.5 and GII.20
title_sort Wastewater surveillance of enteric viruses in eastern Argentina: High rates of detection and first report of NoV GI.5 and GII.20
dc.creator.none.fl_str_mv Frydman, Camila Ayelen
Miño, S.
Iglesias, N. G.
Carballeda, J. M.
Simari, Milagros Belén
Pisano, María Belén
Dus Santos, María José
Mozgovoj, Marina Valeria
author Frydman, Camila Ayelen
author_facet Frydman, Camila Ayelen
Miño, S.
Iglesias, N. G.
Carballeda, J. M.
Simari, Milagros Belén
Pisano, María Belén
Dus Santos, María José
Mozgovoj, Marina Valeria
author_role author
author2 Miño, S.
Iglesias, N. G.
Carballeda, J. M.
Simari, Milagros Belén
Pisano, María Belén
Dus Santos, María José
Mozgovoj, Marina Valeria
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv SEWAGE
ENTERICVIRUSES
PHYLOGENETIC ANALYSIS
NOROVIRUS
topic SEWAGE
ENTERICVIRUSES
PHYLOGENETIC ANALYSIS
NOROVIRUS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.7
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Individuals infected with enteric viruses excrete them in their feces for an extended period, whether symptomatic or asymptomatic. This characteristic, combined with the capability of these viruses to persist in the environment, forms the core of our research. The objective of our study was to investigate the presence of viruses associated with diarrhea and hepatitis: Hepatitis A virus (HAV), Hepatitis E virus (HEV), Norovirus GI (NoV GI), Norovirus GII (NoV GII), and Rotavirus (RV) by RT-real time PCR, in untreated wastewater samples (n=100) collected in the period July 2020 to August 2021 from two low-income neighborhoods in the district of La Plata, Buenos Aires, Argentina. Globally, the percentage of positive samples was 25 % for HEV, 27 % for RV, 14 % for NoV GII, 1 % for NoV GI, and no detectable samples were found for HAV. HEV, RV, and NoV GII were detected in most of the studied months, with the highest detection rates of RV and NoV GII during the winter season. Regarding RV positive samples, the gene encoding the VP8* protein of three samples was sequenced and phylogenetic analysis revealed that the detected strains belonged to genotypes P[8] and P[3]. Additionally, four NoV strains were also genetically characterized by amplifying a fragment corresponding to the ORF-1/ORF-2 junction region. The identified strains were NoV GI.5, NoV GII.4, NoV GII.17 and NoV GII.20. Our results provide relevant information and serve as scientific evidence of the importance of considering wastewater analysis as a feasible strategy to determine the circulation of enteric viruses in the population, with the further benefit of predicting emerging strains. Moreover, this study represents the first report of the circulation of NoV GII.20 and GI.5 genotypes in our country.
Fil: Frydman, Camila Ayelen. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Agroindustria. Instituto de Tecnología de Alimentos. Instituto de Ciencia y Tecnología de Sistemas Alimentarios Sustentables. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología de Sistemas Alimentarios Sustentables; Argentina. Universidad Nacional de Hurlingham.; Argentina
Fil: Miño, S.. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Misiones. Estacion Experimental Agropecuaria Cerro Azul.; Argentina
Fil: Iglesias, N. G.. Universidad Nacional de Hurlingham.; Argentina
Fil: Carballeda, J. M.. Universidad Nacional de Hurlingham.; Argentina
Fil: Simari, Milagros Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; Argentina
Fil: Pisano, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina
Fil: Dus Santos, María José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología e Innovaciones Tecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Virología e Innovaciones Tecnológicas; Argentina. Universidad Nacional de Hurlingham.; Argentina
Fil: Mozgovoj, Marina Valeria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Agroindustria. Instituto de Tecnología de Alimentos. Instituto de Ciencia y Tecnología de Sistemas Alimentarios Sustentables. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología de Sistemas Alimentarios Sustentables; Argentina. Universidad Nacional de Hurlingham.; Argentina
description Individuals infected with enteric viruses excrete them in their feces for an extended period, whether symptomatic or asymptomatic. This characteristic, combined with the capability of these viruses to persist in the environment, forms the core of our research. The objective of our study was to investigate the presence of viruses associated with diarrhea and hepatitis: Hepatitis A virus (HAV), Hepatitis E virus (HEV), Norovirus GI (NoV GI), Norovirus GII (NoV GII), and Rotavirus (RV) by RT-real time PCR, in untreated wastewater samples (n=100) collected in the period July 2020 to August 2021 from two low-income neighborhoods in the district of La Plata, Buenos Aires, Argentina. Globally, the percentage of positive samples was 25 % for HEV, 27 % for RV, 14 % for NoV GII, 1 % for NoV GI, and no detectable samples were found for HAV. HEV, RV, and NoV GII were detected in most of the studied months, with the highest detection rates of RV and NoV GII during the winter season. Regarding RV positive samples, the gene encoding the VP8* protein of three samples was sequenced and phylogenetic analysis revealed that the detected strains belonged to genotypes P[8] and P[3]. Additionally, four NoV strains were also genetically characterized by amplifying a fragment corresponding to the ORF-1/ORF-2 junction region. The identified strains were NoV GI.5, NoV GII.4, NoV GII.17 and NoV GII.20. Our results provide relevant information and serve as scientific evidence of the importance of considering wastewater analysis as a feasible strategy to determine the circulation of enteric viruses in the population, with the further benefit of predicting emerging strains. Moreover, this study represents the first report of the circulation of NoV GII.20 and GI.5 genotypes in our country.
publishDate 2024
dc.date.none.fl_str_mv 2024-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/231049
Frydman, Camila Ayelen; Miño, S.; Iglesias, N. G.; Carballeda, J. M.; Simari, Milagros Belén; et al.; Wastewater surveillance of enteric viruses in eastern Argentina: High rates of detection and first report of NoV GI.5 and GII.20; Elsevier; Environmental Advances; 15; 4-2024; 1-10
2666-7657
CONICET Digital
CONICET
url http://hdl.handle.net/11336/231049
identifier_str_mv Frydman, Camila Ayelen; Miño, S.; Iglesias, N. G.; Carballeda, J. M.; Simari, Milagros Belén; et al.; Wastewater surveillance of enteric viruses in eastern Argentina: High rates of detection and first report of NoV GI.5 and GII.20; Elsevier; Environmental Advances; 15; 4-2024; 1-10
2666-7657
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S266676572400019X
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envadv.2024.100501
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269953445593088
score 13.13397

Publicaciones similares