Enteric virus presence in green vegetables and associated irrigation waters in a rural area from Argentina: A quantitative microbial risk assessment

Autores
Prez, Verónica Emilse; Matías, Victoria; Martinez, Cecilia Laura; Giordano, Miguel Oscar; Masachessi, Gisela; Di Cola Bucciarelli, Guadalupe; Ré, Viviana Elizabeth; Paván, Victorio Jorge; Rodney, Colina; Nates, Silvia Viviana; Barril, Patricia Angelica
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study was to assess the presence of norovirus, rotavirus and infective enterovirus in leafy green vegetables and irrigation waters collected from a farm located at the province of Córdoba, Argentina, and to estimate the quantitative risk of infection by consuming these vegetables. During June 2014–July 2015, vegetables (n = 101) and their corresponding irrigation waters (n = 24) were collected. Viruses were concentrated in both matrices by polyethylene glycol precipitation and then were subjected to RT-PCR to assess the presence of norovirus and rotavirus. The concentrates were also inoculated in CaCo-2 cells to monitor the occurrence of infective enterovirus. The frequency of detection of norovirus, rotavirus and infective enterovirus in irrigation waters was 37.5%, 20.8% and 37.5% and in crops 60.4%, 22.7% and 35.6% respectively. Similar profiles of norovirus genogroups and rotavirus G-types distribution were observed in green vegetables and irrigation waters. The estimated risk of rotavirus infection associated with raw consumption of the vegetables harvested in that rural farm was 0.2 per person per day. This study demonstrates a wide distribution of human pathogenic viruses in irrigation waters and green leafy vegetables, which is of concern when, as in this case, the vegetables are eaten raw.
Fil: Prez, Verónica Emilse. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Matías, Victoria. Universidad de la República. Centro Universitario del Litoral Norte. Centro Universitario de Salto; Uruguay
Fil: Martinez, Cecilia Laura. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina
Fil: Giordano, Miguel Oscar. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina
Fil: Masachessi, Gisela. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Di Cola Bucciarelli, Guadalupe. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Ré, Viviana Elizabeth. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Paván, Victorio Jorge. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina
Fil: Rodney, Colina. Universidad de la República. Centro Universitario del Litoral Norte. Centro Universitario de Salto; Uruguay
Fil: Nates, Silvia Viviana. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina
Fil: Barril, Patricia Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Instituto Nacional de Tecnología Industrial. Centro de Investigación y Asistencia Técnica a la Industria Villa Regina; Argentina
Materia
ENTEROVIRUS
FOODBORNE
NOROVIRUS
QMRA
ROTAVIRUS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/150012
Acceder
id CONICETDig_84f362ce951ff4264541c5edbb304c82
oai_identifier_str oai:ri.conicet.gov.ar:11336/150012
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Enteric virus presence in green vegetables and associated irrigation waters in a rural area from Argentina: A quantitative microbial risk assessmentPrez, Verónica EmilseMatías, VictoriaMartinez, Cecilia LauraGiordano, Miguel OscarMasachessi, GiselaDi Cola Bucciarelli, GuadalupeRé, Viviana ElizabethPaván, Victorio JorgeRodney, ColinaNates, Silvia VivianaBarril, Patricia AngelicaENTEROVIRUSFOODBORNENOROVIRUSQMRAROTAVIRUShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The aim of this study was to assess the presence of norovirus, rotavirus and infective enterovirus in leafy green vegetables and irrigation waters collected from a farm located at the province of Córdoba, Argentina, and to estimate the quantitative risk of infection by consuming these vegetables. During June 2014–July 2015, vegetables (n = 101) and their corresponding irrigation waters (n = 24) were collected. Viruses were concentrated in both matrices by polyethylene glycol precipitation and then were subjected to RT-PCR to assess the presence of norovirus and rotavirus. The concentrates were also inoculated in CaCo-2 cells to monitor the occurrence of infective enterovirus. The frequency of detection of norovirus, rotavirus and infective enterovirus in irrigation waters was 37.5%, 20.8% and 37.5% and in crops 60.4%, 22.7% and 35.6% respectively. Similar profiles of norovirus genogroups and rotavirus G-types distribution were observed in green vegetables and irrigation waters. The estimated risk of rotavirus infection associated with raw consumption of the vegetables harvested in that rural farm was 0.2 per person per day. This study demonstrates a wide distribution of human pathogenic viruses in irrigation waters and green leafy vegetables, which is of concern when, as in this case, the vegetables are eaten raw.Fil: Prez, Verónica Emilse. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Matías, Victoria. Universidad de la República. Centro Universitario del Litoral Norte. Centro Universitario de Salto; UruguayFil: Martinez, Cecilia Laura. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; ArgentinaFil: Giordano, Miguel Oscar. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; ArgentinaFil: Masachessi, Gisela. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Di Cola Bucciarelli, Guadalupe. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Ré, Viviana Elizabeth. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Paván, Victorio Jorge. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; ArgentinaFil: Rodney, Colina. Universidad de la República. Centro Universitario del Litoral Norte. Centro Universitario de Salto; UruguayFil: Nates, Silvia Viviana. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; ArgentinaFil: Barril, Patricia Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Instituto Nacional de Tecnología Industrial. Centro de Investigación y Asistencia Técnica a la Industria Villa Regina; ArgentinaElsevier Science2021-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/150012Prez, Verónica Emilse; Matías, Victoria; Martinez, Cecilia Laura; Giordano, Miguel Oscar; Masachessi, Gisela; et al.; Enteric virus presence in green vegetables and associated irrigation waters in a rural area from Argentina: A quantitative microbial risk assessment; Elsevier Science; LWT - Food Science and Technology; 144; 6-2021; 1-70023-64381096-1127CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0023643821003546?via%3Dihubinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.lwt.2021.111201info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:56:00Zoai:ri.conicet.gov.ar:11336/150012instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:56:00.478CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Enteric virus presence in green vegetables and associated irrigation waters in a rural area from Argentina: A quantitative microbial risk assessment
title Enteric virus presence in green vegetables and associated irrigation waters in a rural area from Argentina: A quantitative microbial risk assessment
spellingShingle Enteric virus presence in green vegetables and associated irrigation waters in a rural area from Argentina: A quantitative microbial risk assessment
Prez, Verónica Emilse
ENTEROVIRUS
FOODBORNE
NOROVIRUS
QMRA
ROTAVIRUS
title_short Enteric virus presence in green vegetables and associated irrigation waters in a rural area from Argentina: A quantitative microbial risk assessment
title_full Enteric virus presence in green vegetables and associated irrigation waters in a rural area from Argentina: A quantitative microbial risk assessment
title_fullStr Enteric virus presence in green vegetables and associated irrigation waters in a rural area from Argentina: A quantitative microbial risk assessment
title_full_unstemmed Enteric virus presence in green vegetables and associated irrigation waters in a rural area from Argentina: A quantitative microbial risk assessment
title_sort Enteric virus presence in green vegetables and associated irrigation waters in a rural area from Argentina: A quantitative microbial risk assessment
dc.creator.none.fl_str_mv Prez, Verónica Emilse
Matías, Victoria
Martinez, Cecilia Laura
Giordano, Miguel Oscar
Masachessi, Gisela
Di Cola Bucciarelli, Guadalupe
Ré, Viviana Elizabeth
Paván, Victorio Jorge
Rodney, Colina
Nates, Silvia Viviana
Barril, Patricia Angelica
author Prez, Verónica Emilse
author_facet Prez, Verónica Emilse
Matías, Victoria
Martinez, Cecilia Laura
Giordano, Miguel Oscar
Masachessi, Gisela
Di Cola Bucciarelli, Guadalupe
Ré, Viviana Elizabeth
Paván, Victorio Jorge
Rodney, Colina
Nates, Silvia Viviana
Barril, Patricia Angelica
author_role author
author2 Matías, Victoria
Martinez, Cecilia Laura
Giordano, Miguel Oscar
Masachessi, Gisela
Di Cola Bucciarelli, Guadalupe
Ré, Viviana Elizabeth
Paván, Victorio Jorge
Rodney, Colina
Nates, Silvia Viviana
Barril, Patricia Angelica
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ENTEROVIRUS
FOODBORNE
NOROVIRUS
QMRA
ROTAVIRUS
topic ENTEROVIRUS
FOODBORNE
NOROVIRUS
QMRA
ROTAVIRUS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The aim of this study was to assess the presence of norovirus, rotavirus and infective enterovirus in leafy green vegetables and irrigation waters collected from a farm located at the province of Córdoba, Argentina, and to estimate the quantitative risk of infection by consuming these vegetables. During June 2014–July 2015, vegetables (n = 101) and their corresponding irrigation waters (n = 24) were collected. Viruses were concentrated in both matrices by polyethylene glycol precipitation and then were subjected to RT-PCR to assess the presence of norovirus and rotavirus. The concentrates were also inoculated in CaCo-2 cells to monitor the occurrence of infective enterovirus. The frequency of detection of norovirus, rotavirus and infective enterovirus in irrigation waters was 37.5%, 20.8% and 37.5% and in crops 60.4%, 22.7% and 35.6% respectively. Similar profiles of norovirus genogroups and rotavirus G-types distribution were observed in green vegetables and irrigation waters. The estimated risk of rotavirus infection associated with raw consumption of the vegetables harvested in that rural farm was 0.2 per person per day. This study demonstrates a wide distribution of human pathogenic viruses in irrigation waters and green leafy vegetables, which is of concern when, as in this case, the vegetables are eaten raw.
Fil: Prez, Verónica Emilse. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Matías, Victoria. Universidad de la República. Centro Universitario del Litoral Norte. Centro Universitario de Salto; Uruguay
Fil: Martinez, Cecilia Laura. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina
Fil: Giordano, Miguel Oscar. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina
Fil: Masachessi, Gisela. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Di Cola Bucciarelli, Guadalupe. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Ré, Viviana Elizabeth. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Paván, Victorio Jorge. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina
Fil: Rodney, Colina. Universidad de la República. Centro Universitario del Litoral Norte. Centro Universitario de Salto; Uruguay
Fil: Nates, Silvia Viviana. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina
Fil: Barril, Patricia Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Instituto Nacional de Tecnología Industrial. Centro de Investigación y Asistencia Técnica a la Industria Villa Regina; Argentina
description The aim of this study was to assess the presence of norovirus, rotavirus and infective enterovirus in leafy green vegetables and irrigation waters collected from a farm located at the province of Córdoba, Argentina, and to estimate the quantitative risk of infection by consuming these vegetables. During June 2014–July 2015, vegetables (n = 101) and their corresponding irrigation waters (n = 24) were collected. Viruses were concentrated in both matrices by polyethylene glycol precipitation and then were subjected to RT-PCR to assess the presence of norovirus and rotavirus. The concentrates were also inoculated in CaCo-2 cells to monitor the occurrence of infective enterovirus. The frequency of detection of norovirus, rotavirus and infective enterovirus in irrigation waters was 37.5%, 20.8% and 37.5% and in crops 60.4%, 22.7% and 35.6% respectively. Similar profiles of norovirus genogroups and rotavirus G-types distribution were observed in green vegetables and irrigation waters. The estimated risk of rotavirus infection associated with raw consumption of the vegetables harvested in that rural farm was 0.2 per person per day. This study demonstrates a wide distribution of human pathogenic viruses in irrigation waters and green leafy vegetables, which is of concern when, as in this case, the vegetables are eaten raw.
publishDate 2021
dc.date.none.fl_str_mv 2021-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/150012
Prez, Verónica Emilse; Matías, Victoria; Martinez, Cecilia Laura; Giordano, Miguel Oscar; Masachessi, Gisela; et al.; Enteric virus presence in green vegetables and associated irrigation waters in a rural area from Argentina: A quantitative microbial risk assessment; Elsevier Science; LWT - Food Science and Technology; 144; 6-2021; 1-7
0023-6438
1096-1127
CONICET Digital
CONICET
url http://hdl.handle.net/11336/150012
identifier_str_mv Prez, Verónica Emilse; Matías, Victoria; Martinez, Cecilia Laura; Giordano, Miguel Oscar; Masachessi, Gisela; et al.; Enteric virus presence in green vegetables and associated irrigation waters in a rural area from Argentina: A quantitative microbial risk assessment; Elsevier Science; LWT - Food Science and Technology; 144; 6-2021; 1-7
0023-6438
1096-1127
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0023643821003546?via%3Dihub
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.lwt.2021.111201
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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