Trichothecene genotype profiling of wheat Fusarium graminearum species complex in Paraguay

Autores
Arrua Alvarenga, Andrea Alejandra; Iehisa Ouchi, Julio César Masaru; Cazal Martínez, Cinthia Carolina; Moura Mendes, Juliana; Colmán, Adans Agustín; Fernández Ríos, Danilo; Arrua, Pablo David; Barboza Guerreño, Claudia Adriana; Kohli, Man Mohan; Ramírez, María Laura; Acuña Ruíz, Ana; Sarmiento, María Magdalena; Ortíz, María Cecilia; Nuñez Quiles, Adriana Elizabeth; Lopez-Nicora, Horacio D.
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Paraguay is a non-traditional wheat-producing country in one of the warmest regions in South America. Fusarium Head Blight (FHB) is a critical disease affecting this crop, caused by the Fusarium graminearum species complex (FGSC). A variety of these species produce trichothecenes, including deoxynivalenol (DON) and its acetylated forms (3-ADON and 15-ADON) or nivalenol (NIV). This study characterized the phylogenetic relationships, and chemotype diversity of 28 strains within FGSC collected from wheat fields across different country regions. Phylogenetic analysis based on the sequence of elongation factor-1α gene (EF-1α) from 28 strains revealed the presence of four species in the FGSC: F. graminearum sensu stricto, F. asiaticum, F. meridionale and F. cortaderiae. Ten strains selected for further analysis revealed that all F. graminearum strains were 15-ADON chemotype, while the two strains of F. meridionale and one strain of F. asiaticum were NIV chemotype. Thus, the 15-ADON chemotype of F. graminearum sensu stricto was predominant within the Fusarium strains isolated in the country. This work is the first report of phylogenetic relationships and chemotype diversity among Fusarium strains which will help understand the population diversity of this pathogen in Paraguay.
Fil: Arrua Alvarenga, Andrea Alejandra. Universidad Nacional de Asunción; Paraguay
Fil: Iehisa Ouchi, Julio César Masaru. Universidad Nacional de Asunción; Paraguay
Fil: Cazal Martínez, Cinthia Carolina. Universidad Nacional de Asunción; Paraguay
Fil: Moura Mendes, Juliana. Universidad Nacional de Asunción; Paraguay
Fil: Colmán, Adans Agustín. Universidad Nacional de Asunción; Paraguay
Fil: Fernández Ríos, Danilo. Universidad Nacional de Asunción; Paraguay
Fil: Arrua, Pablo David. Universidad Nacional de Asunción; Paraguay
Fil: Barboza Guerreño, Claudia Adriana. Universidad Nacional de Asunción; Paraguay
Fil: Kohli, Man Mohan. Cámara Paraguaya de Exportadores y Comercializadores de; Paraguay
Fil: Ramírez, María Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Acuña Ruíz, Ana. Universidad Nacional de Asunción; Paraguay
Fil: Sarmiento, María Magdalena. Universidad Nacional de Asunción; Paraguay
Fil: Ortíz, María Cecilia. Universidad Nacional de Asunción; Paraguay
Fil: Nuñez Quiles, Adriana Elizabeth. Universidad Nacional de Asunción; Paraguay
Fil: Lopez-Nicora, Horacio D.. Ohio University; Estados Unidos
Materia
CEREAL
FOOD SAFETY
MYCOTOXINS
TRITICUM AESTIVUM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/162988
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/162988
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Trichothecene genotype profiling of wheat Fusarium graminearum species complex in ParaguayArrua Alvarenga, Andrea AlejandraIehisa Ouchi, Julio César MasaruCazal Martínez, Cinthia CarolinaMoura Mendes, JulianaColmán, Adans AgustínFernández Ríos, DaniloArrua, Pablo DavidBarboza Guerreño, Claudia AdrianaKohli, Man MohanRamírez, María LauraAcuña Ruíz, AnaSarmiento, María MagdalenaOrtíz, María CeciliaNuñez Quiles, Adriana ElizabethLopez-Nicora, Horacio D.CEREALFOOD SAFETYMYCOTOXINSTRITICUM AESTIVUMhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Paraguay is a non-traditional wheat-producing country in one of the warmest regions in South America. Fusarium Head Blight (FHB) is a critical disease affecting this crop, caused by the Fusarium graminearum species complex (FGSC). A variety of these species produce trichothecenes, including deoxynivalenol (DON) and its acetylated forms (3-ADON and 15-ADON) or nivalenol (NIV). This study characterized the phylogenetic relationships, and chemotype diversity of 28 strains within FGSC collected from wheat fields across different country regions. Phylogenetic analysis based on the sequence of elongation factor-1α gene (EF-1α) from 28 strains revealed the presence of four species in the FGSC: F. graminearum sensu stricto, F. asiaticum, F. meridionale and F. cortaderiae. Ten strains selected for further analysis revealed that all F. graminearum strains were 15-ADON chemotype, while the two strains of F. meridionale and one strain of F. asiaticum were NIV chemotype. Thus, the 15-ADON chemotype of F. graminearum sensu stricto was predominant within the Fusarium strains isolated in the country. This work is the first report of phylogenetic relationships and chemotype diversity among Fusarium strains which will help understand the population diversity of this pathogen in Paraguay.Fil: Arrua Alvarenga, Andrea Alejandra. Universidad Nacional de Asunción; ParaguayFil: Iehisa Ouchi, Julio César Masaru. Universidad Nacional de Asunción; ParaguayFil: Cazal Martínez, Cinthia Carolina. Universidad Nacional de Asunción; ParaguayFil: Moura Mendes, Juliana. Universidad Nacional de Asunción; ParaguayFil: Colmán, Adans Agustín. Universidad Nacional de Asunción; ParaguayFil: Fernández Ríos, Danilo. Universidad Nacional de Asunción; ParaguayFil: Arrua, Pablo David. Universidad Nacional de Asunción; ParaguayFil: Barboza Guerreño, Claudia Adriana. Universidad Nacional de Asunción; ParaguayFil: Kohli, Man Mohan. Cámara Paraguaya de Exportadores y Comercializadores de; ParaguayFil: Ramírez, María Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; ArgentinaFil: Acuña Ruíz, Ana. Universidad Nacional de Asunción; ParaguayFil: Sarmiento, María Magdalena. Universidad Nacional de Asunción; ParaguayFil: Ortíz, María Cecilia. Universidad Nacional de Asunción; ParaguayFil: Nuñez Quiles, Adriana Elizabeth. Universidad Nacional de Asunción; ParaguayFil: Lopez-Nicora, Horacio D.. Ohio University; Estados UnidosMDPI2022-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/162988Arrua Alvarenga, Andrea Alejandra; Iehisa Ouchi, Julio César Masaru; Cazal Martínez, Cinthia Carolina; Moura Mendes, Juliana; Colmán, Adans Agustín; et al.; Trichothecene genotype profiling of wheat Fusarium graminearum species complex in Paraguay; MDPI; Toxins; 14; 4; 4-2022; 1-102072-6651CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2072-6651/14/4/257info:eu-repo/semantics/altIdentifier/doi/10.3390/toxins14040257info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T11:28:24Zoai:ri.conicet.gov.ar:11336/162988instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 11:28:24.603CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Trichothecene genotype profiling of wheat Fusarium graminearum species complex in Paraguay
title Trichothecene genotype profiling of wheat Fusarium graminearum species complex in Paraguay
spellingShingle Trichothecene genotype profiling of wheat Fusarium graminearum species complex in Paraguay
Arrua Alvarenga, Andrea Alejandra
CEREAL
FOOD SAFETY
MYCOTOXINS
TRITICUM AESTIVUM
title_short Trichothecene genotype profiling of wheat Fusarium graminearum species complex in Paraguay
title_full Trichothecene genotype profiling of wheat Fusarium graminearum species complex in Paraguay
title_fullStr Trichothecene genotype profiling of wheat Fusarium graminearum species complex in Paraguay
title_full_unstemmed Trichothecene genotype profiling of wheat Fusarium graminearum species complex in Paraguay
title_sort Trichothecene genotype profiling of wheat Fusarium graminearum species complex in Paraguay
dc.creator.none.fl_str_mv Arrua Alvarenga, Andrea Alejandra
Iehisa Ouchi, Julio César Masaru
Cazal Martínez, Cinthia Carolina
Moura Mendes, Juliana
Colmán, Adans Agustín
Fernández Ríos, Danilo
Arrua, Pablo David
Barboza Guerreño, Claudia Adriana
Kohli, Man Mohan
Ramírez, María Laura
Acuña Ruíz, Ana
Sarmiento, María Magdalena
Ortíz, María Cecilia
Nuñez Quiles, Adriana Elizabeth
Lopez-Nicora, Horacio D.
author Arrua Alvarenga, Andrea Alejandra
author_facet Arrua Alvarenga, Andrea Alejandra
Iehisa Ouchi, Julio César Masaru
Cazal Martínez, Cinthia Carolina
Moura Mendes, Juliana
Colmán, Adans Agustín
Fernández Ríos, Danilo
Arrua, Pablo David
Barboza Guerreño, Claudia Adriana
Kohli, Man Mohan
Ramírez, María Laura
Acuña Ruíz, Ana
Sarmiento, María Magdalena
Ortíz, María Cecilia
Nuñez Quiles, Adriana Elizabeth
Lopez-Nicora, Horacio D.
author_role author
author2 Iehisa Ouchi, Julio César Masaru
Cazal Martínez, Cinthia Carolina
Moura Mendes, Juliana
Colmán, Adans Agustín
Fernández Ríos, Danilo
Arrua, Pablo David
Barboza Guerreño, Claudia Adriana
Kohli, Man Mohan
Ramírez, María Laura
Acuña Ruíz, Ana
Sarmiento, María Magdalena
Ortíz, María Cecilia
Nuñez Quiles, Adriana Elizabeth
Lopez-Nicora, Horacio D.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CEREAL
FOOD SAFETY
MYCOTOXINS
TRITICUM AESTIVUM
topic CEREAL
FOOD SAFETY
MYCOTOXINS
TRITICUM AESTIVUM
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Paraguay is a non-traditional wheat-producing country in one of the warmest regions in South America. Fusarium Head Blight (FHB) is a critical disease affecting this crop, caused by the Fusarium graminearum species complex (FGSC). A variety of these species produce trichothecenes, including deoxynivalenol (DON) and its acetylated forms (3-ADON and 15-ADON) or nivalenol (NIV). This study characterized the phylogenetic relationships, and chemotype diversity of 28 strains within FGSC collected from wheat fields across different country regions. Phylogenetic analysis based on the sequence of elongation factor-1α gene (EF-1α) from 28 strains revealed the presence of four species in the FGSC: F. graminearum sensu stricto, F. asiaticum, F. meridionale and F. cortaderiae. Ten strains selected for further analysis revealed that all F. graminearum strains were 15-ADON chemotype, while the two strains of F. meridionale and one strain of F. asiaticum were NIV chemotype. Thus, the 15-ADON chemotype of F. graminearum sensu stricto was predominant within the Fusarium strains isolated in the country. This work is the first report of phylogenetic relationships and chemotype diversity among Fusarium strains which will help understand the population diversity of this pathogen in Paraguay.
Fil: Arrua Alvarenga, Andrea Alejandra. Universidad Nacional de Asunción; Paraguay
Fil: Iehisa Ouchi, Julio César Masaru. Universidad Nacional de Asunción; Paraguay
Fil: Cazal Martínez, Cinthia Carolina. Universidad Nacional de Asunción; Paraguay
Fil: Moura Mendes, Juliana. Universidad Nacional de Asunción; Paraguay
Fil: Colmán, Adans Agustín. Universidad Nacional de Asunción; Paraguay
Fil: Fernández Ríos, Danilo. Universidad Nacional de Asunción; Paraguay
Fil: Arrua, Pablo David. Universidad Nacional de Asunción; Paraguay
Fil: Barboza Guerreño, Claudia Adriana. Universidad Nacional de Asunción; Paraguay
Fil: Kohli, Man Mohan. Cámara Paraguaya de Exportadores y Comercializadores de; Paraguay
Fil: Ramírez, María Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Acuña Ruíz, Ana. Universidad Nacional de Asunción; Paraguay
Fil: Sarmiento, María Magdalena. Universidad Nacional de Asunción; Paraguay
Fil: Ortíz, María Cecilia. Universidad Nacional de Asunción; Paraguay
Fil: Nuñez Quiles, Adriana Elizabeth. Universidad Nacional de Asunción; Paraguay
Fil: Lopez-Nicora, Horacio D.. Ohio University; Estados Unidos
description Paraguay is a non-traditional wheat-producing country in one of the warmest regions in South America. Fusarium Head Blight (FHB) is a critical disease affecting this crop, caused by the Fusarium graminearum species complex (FGSC). A variety of these species produce trichothecenes, including deoxynivalenol (DON) and its acetylated forms (3-ADON and 15-ADON) or nivalenol (NIV). This study characterized the phylogenetic relationships, and chemotype diversity of 28 strains within FGSC collected from wheat fields across different country regions. Phylogenetic analysis based on the sequence of elongation factor-1α gene (EF-1α) from 28 strains revealed the presence of four species in the FGSC: F. graminearum sensu stricto, F. asiaticum, F. meridionale and F. cortaderiae. Ten strains selected for further analysis revealed that all F. graminearum strains were 15-ADON chemotype, while the two strains of F. meridionale and one strain of F. asiaticum were NIV chemotype. Thus, the 15-ADON chemotype of F. graminearum sensu stricto was predominant within the Fusarium strains isolated in the country. This work is the first report of phylogenetic relationships and chemotype diversity among Fusarium strains which will help understand the population diversity of this pathogen in Paraguay.
publishDate 2022
dc.date.none.fl_str_mv 2022-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/162988
Arrua Alvarenga, Andrea Alejandra; Iehisa Ouchi, Julio César Masaru; Cazal Martínez, Cinthia Carolina; Moura Mendes, Juliana; Colmán, Adans Agustín; et al.; Trichothecene genotype profiling of wheat Fusarium graminearum species complex in Paraguay; MDPI; Toxins; 14; 4; 4-2022; 1-10
2072-6651
CONICET Digital
CONICET
url http://hdl.handle.net/11336/162988
identifier_str_mv Arrua Alvarenga, Andrea Alejandra; Iehisa Ouchi, Julio César Masaru; Cazal Martínez, Cinthia Carolina; Moura Mendes, Juliana; Colmán, Adans Agustín; et al.; Trichothecene genotype profiling of wheat Fusarium graminearum species complex in Paraguay; MDPI; Toxins; 14; 4; 4-2022; 1-10
2072-6651
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2072-6651/14/4/257
info:eu-repo/semantics/altIdentifier/doi/10.3390/toxins14040257
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.000565

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