Genetic population structure and trichothecene genotypes of Fusarium graminearum isolated from wheat in southern Brazil

Autores
Astolfi, P.; Reynoso, Maria Marta; Ramirez, Maria Laura; Chulze, Sofia Noemi; Alves, T. C. A.; Tessmann, D. J.; Del Ponte, E. M.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A sample of 140 Fusarium graminearum isolates from Rio Grande do Sul, Brazil, representing three populations at least 150km from one another, were examined for trichothecene genotype based on PCR amplification of portions of the Tri3 and Tri12 genes and a species-specific (Fg16F/R) primer pair. Genetic diversity was assessed in a sample of 103 F. graminearum lineage 7 (F. graminearum sensu stricto) isolates using amplified fragment length polymorphism (AFLP) markers. The 15-ADON genotype was dominant, followed by the NIV genotype (2-18% prevalence), across all three populations. All NIV-type isolates were in lineage 2 (F. meridionale) and all 15-ADON-type isolates were in lineage 7. Isolates with the same haplotype were rare and genotypic diversity was uniformly high (≥98% of the count), suggesting that recombination has played a significant role. The number of migrants (N m) was estimated between 5 and 6 across all loci and all populations, but the high frequency of private alleles (up to 30%) suggests a historical, rather than contemporary, gene flow. Regarding linkage disequilibrium, 0·8, 1·5 and 2·2% of the locus pairs from the three populations were in disequilibrium, which is lower than values reported in other locations. Thus, Brazilian populations differ from those found in Europe, North America and most of Asia in the presence of a significant frequency (7·8%) of isolates of the NIV genotype in lineage 2. © 2011 The Authors. Plant Pathology © 2011 BSPP.
Fil: Astolfi, P.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Reynoso, Maria Marta. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Ramirez, Maria Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Chulze, Sofia Noemi. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Alves, T. C. A.. Universidade Estadual de Maringá; Brasil
Fil: Tessmann, D. J.. Universidade Estadual de Maringá; Brasil
Fil: Del Ponte, E. M.. Universidade Federal do Rio Grande do Sul; Brasil
Materia
CHEMOTYPE,
DEOXYNIVALENOL,
GIBBERELLA ZEAE,
SMALL GRAINS,
TRITICUM AESTIVUM,
WHEAT SCAB
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/189135
Acceder
id CONICETDig_1145ab0b9c714122224c9e939a023db1
oai_identifier_str oai:ri.conicet.gov.ar:11336/189135
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genetic population structure and trichothecene genotypes of Fusarium graminearum isolated from wheat in southern BrazilAstolfi, P.Reynoso, Maria MartaRamirez, Maria LauraChulze, Sofia NoemiAlves, T. C. A.Tessmann, D. J.Del Ponte, E. M.CHEMOTYPE,DEOXYNIVALENOL,GIBBERELLA ZEAE,SMALL GRAINS,TRITICUM AESTIVUM,WHEAT SCABhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A sample of 140 Fusarium graminearum isolates from Rio Grande do Sul, Brazil, representing three populations at least 150km from one another, were examined for trichothecene genotype based on PCR amplification of portions of the Tri3 and Tri12 genes and a species-specific (Fg16F/R) primer pair. Genetic diversity was assessed in a sample of 103 F. graminearum lineage 7 (F. graminearum sensu stricto) isolates using amplified fragment length polymorphism (AFLP) markers. The 15-ADON genotype was dominant, followed by the NIV genotype (2-18% prevalence), across all three populations. All NIV-type isolates were in lineage 2 (F. meridionale) and all 15-ADON-type isolates were in lineage 7. Isolates with the same haplotype were rare and genotypic diversity was uniformly high (≥98% of the count), suggesting that recombination has played a significant role. The number of migrants (N m) was estimated between 5 and 6 across all loci and all populations, but the high frequency of private alleles (up to 30%) suggests a historical, rather than contemporary, gene flow. Regarding linkage disequilibrium, 0·8, 1·5 and 2·2% of the locus pairs from the three populations were in disequilibrium, which is lower than values reported in other locations. Thus, Brazilian populations differ from those found in Europe, North America and most of Asia in the presence of a significant frequency (7·8%) of isolates of the NIV genotype in lineage 2. © 2011 The Authors. Plant Pathology © 2011 BSPP.Fil: Astolfi, P.. Universidade Federal do Rio Grande do Sul; BrasilFil: Reynoso, Maria Marta. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; ArgentinaFil: Ramirez, Maria Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Chulze, Sofia Noemi. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Alves, T. C. A.. Universidade Estadual de Maringá; BrasilFil: Tessmann, D. J.. Universidade Estadual de Maringá; BrasilFil: Del Ponte, E. M.. Universidade Federal do Rio Grande do Sul; BrasilWiley Blackwell Publishing, Inc2012-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/189135Astolfi, P.; Reynoso, Maria Marta; Ramirez, Maria Laura; Chulze, Sofia Noemi; Alves, T. C. A.; et al.; Genetic population structure and trichothecene genotypes of Fusarium graminearum isolated from wheat in southern Brazil; Wiley Blackwell Publishing, Inc; Plant Pathology; 61; 2; 7-2012; 289-2950032-0862CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-3059.2011.02515.xinfo:eu-repo/semantics/altIdentifier/url/https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/j.1365-3059.2011.02515.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:54:33Zoai:ri.conicet.gov.ar:11336/189135instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:54:33.609CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genetic population structure and trichothecene genotypes of Fusarium graminearum isolated from wheat in southern Brazil
title Genetic population structure and trichothecene genotypes of Fusarium graminearum isolated from wheat in southern Brazil
spellingShingle Genetic population structure and trichothecene genotypes of Fusarium graminearum isolated from wheat in southern Brazil
Astolfi, P.
CHEMOTYPE,
DEOXYNIVALENOL,
GIBBERELLA ZEAE,
SMALL GRAINS,
TRITICUM AESTIVUM,
WHEAT SCAB
title_short Genetic population structure and trichothecene genotypes of Fusarium graminearum isolated from wheat in southern Brazil
title_full Genetic population structure and trichothecene genotypes of Fusarium graminearum isolated from wheat in southern Brazil
title_fullStr Genetic population structure and trichothecene genotypes of Fusarium graminearum isolated from wheat in southern Brazil
title_full_unstemmed Genetic population structure and trichothecene genotypes of Fusarium graminearum isolated from wheat in southern Brazil
title_sort Genetic population structure and trichothecene genotypes of Fusarium graminearum isolated from wheat in southern Brazil
dc.creator.none.fl_str_mv Astolfi, P.
Reynoso, Maria Marta
Ramirez, Maria Laura
Chulze, Sofia Noemi
Alves, T. C. A.
Tessmann, D. J.
Del Ponte, E. M.
author Astolfi, P.
author_facet Astolfi, P.
Reynoso, Maria Marta
Ramirez, Maria Laura
Chulze, Sofia Noemi
Alves, T. C. A.
Tessmann, D. J.
Del Ponte, E. M.
author_role author
author2 Reynoso, Maria Marta
Ramirez, Maria Laura
Chulze, Sofia Noemi
Alves, T. C. A.
Tessmann, D. J.
Del Ponte, E. M.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv CHEMOTYPE,
DEOXYNIVALENOL,
GIBBERELLA ZEAE,
SMALL GRAINS,
TRITICUM AESTIVUM,
WHEAT SCAB
topic CHEMOTYPE,
DEOXYNIVALENOL,
GIBBERELLA ZEAE,
SMALL GRAINS,
TRITICUM AESTIVUM,
WHEAT SCAB
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A sample of 140 Fusarium graminearum isolates from Rio Grande do Sul, Brazil, representing three populations at least 150km from one another, were examined for trichothecene genotype based on PCR amplification of portions of the Tri3 and Tri12 genes and a species-specific (Fg16F/R) primer pair. Genetic diversity was assessed in a sample of 103 F. graminearum lineage 7 (F. graminearum sensu stricto) isolates using amplified fragment length polymorphism (AFLP) markers. The 15-ADON genotype was dominant, followed by the NIV genotype (2-18% prevalence), across all three populations. All NIV-type isolates were in lineage 2 (F. meridionale) and all 15-ADON-type isolates were in lineage 7. Isolates with the same haplotype were rare and genotypic diversity was uniformly high (≥98% of the count), suggesting that recombination has played a significant role. The number of migrants (N m) was estimated between 5 and 6 across all loci and all populations, but the high frequency of private alleles (up to 30%) suggests a historical, rather than contemporary, gene flow. Regarding linkage disequilibrium, 0·8, 1·5 and 2·2% of the locus pairs from the three populations were in disequilibrium, which is lower than values reported in other locations. Thus, Brazilian populations differ from those found in Europe, North America and most of Asia in the presence of a significant frequency (7·8%) of isolates of the NIV genotype in lineage 2. © 2011 The Authors. Plant Pathology © 2011 BSPP.
Fil: Astolfi, P.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Reynoso, Maria Marta. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Ramirez, Maria Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Chulze, Sofia Noemi. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Alves, T. C. A.. Universidade Estadual de Maringá; Brasil
Fil: Tessmann, D. J.. Universidade Estadual de Maringá; Brasil
Fil: Del Ponte, E. M.. Universidade Federal do Rio Grande do Sul; Brasil
description A sample of 140 Fusarium graminearum isolates from Rio Grande do Sul, Brazil, representing three populations at least 150km from one another, were examined for trichothecene genotype based on PCR amplification of portions of the Tri3 and Tri12 genes and a species-specific (Fg16F/R) primer pair. Genetic diversity was assessed in a sample of 103 F. graminearum lineage 7 (F. graminearum sensu stricto) isolates using amplified fragment length polymorphism (AFLP) markers. The 15-ADON genotype was dominant, followed by the NIV genotype (2-18% prevalence), across all three populations. All NIV-type isolates were in lineage 2 (F. meridionale) and all 15-ADON-type isolates were in lineage 7. Isolates with the same haplotype were rare and genotypic diversity was uniformly high (≥98% of the count), suggesting that recombination has played a significant role. The number of migrants (N m) was estimated between 5 and 6 across all loci and all populations, but the high frequency of private alleles (up to 30%) suggests a historical, rather than contemporary, gene flow. Regarding linkage disequilibrium, 0·8, 1·5 and 2·2% of the locus pairs from the three populations were in disequilibrium, which is lower than values reported in other locations. Thus, Brazilian populations differ from those found in Europe, North America and most of Asia in the presence of a significant frequency (7·8%) of isolates of the NIV genotype in lineage 2. © 2011 The Authors. Plant Pathology © 2011 BSPP.
publishDate 2012
dc.date.none.fl_str_mv 2012-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/189135
Astolfi, P.; Reynoso, Maria Marta; Ramirez, Maria Laura; Chulze, Sofia Noemi; Alves, T. C. A.; et al.; Genetic population structure and trichothecene genotypes of Fusarium graminearum isolated from wheat in southern Brazil; Wiley Blackwell Publishing, Inc; Plant Pathology; 61; 2; 7-2012; 289-295
0032-0862
CONICET Digital
CONICET
url http://hdl.handle.net/11336/189135
identifier_str_mv Astolfi, P.; Reynoso, Maria Marta; Ramirez, Maria Laura; Chulze, Sofia Noemi; Alves, T. C. A.; et al.; Genetic population structure and trichothecene genotypes of Fusarium graminearum isolated from wheat in southern Brazil; Wiley Blackwell Publishing, Inc; Plant Pathology; 61; 2; 7-2012; 289-295
0032-0862
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/url/https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/j.1365-3059.2011.02515.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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