Autophagosome Formation Depends on the Small GTPase Rab1 and Functional ER Exit Sites

Autores
Zoppino, Felipe Carlos Martin; Militello, Rodrigo Damián; Slavin, Ileana; Alvarez, Cecilia Ines; Colombo, Maria Isabel
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Autophagy is an important cellular degradation pathway present in all eukaryotic cells. Via this pathway, portions of the cytoplasm and/or organelles are sequestered in double membrane structures called autophagosomes. In spite of the significant advance achieved in autophagy, the long-standing question about the source of the autophagic membrane remains unsolved. We have investigated the role of the secretory pathway in autophagosome biogenesis. Sar1 and Rab1b are monomeric GTPases that control traffic from the ER to the Golgi. We present evidence indicating that the activity of both proteins is required for autophagosome formation. Overexpression of dominant negative mutants and the use of siRNAs impaired autophagosome generation as determined by LC3- puncta formation and LC3-II processing. In addition our results indicate that the autophagic and secretory pathways intersect at a level preceding the brefeldin A blockage, suggesting that transport from the cis/medial Golgi is not necessary for autophagosome biogenesis. Our present results highlight the role of transport from the ER in the initial events of the autophagic vacuole development.
Fil: Zoppino, Felipe Carlos Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Militello, Rodrigo Damián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Slavin, Ileana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Alvarez, Cecilia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Colombo, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Materia
autophagy
Rab1
Sar1
endoplasmic reticulum
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/277457
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/277457
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Autophagosome Formation Depends on the Small GTPase Rab1 and Functional ER Exit SitesZoppino, Felipe Carlos MartinMilitello, Rodrigo DamiánSlavin, IleanaAlvarez, Cecilia InesColombo, Maria IsabelautophagyRab1Sar1endoplasmic reticulumhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Autophagy is an important cellular degradation pathway present in all eukaryotic cells. Via this pathway, portions of the cytoplasm and/or organelles are sequestered in double membrane structures called autophagosomes. In spite of the significant advance achieved in autophagy, the long-standing question about the source of the autophagic membrane remains unsolved. We have investigated the role of the secretory pathway in autophagosome biogenesis. Sar1 and Rab1b are monomeric GTPases that control traffic from the ER to the Golgi. We present evidence indicating that the activity of both proteins is required for autophagosome formation. Overexpression of dominant negative mutants and the use of siRNAs impaired autophagosome generation as determined by LC3- puncta formation and LC3-II processing. In addition our results indicate that the autophagic and secretory pathways intersect at a level preceding the brefeldin A blockage, suggesting that transport from the cis/medial Golgi is not necessary for autophagosome biogenesis. Our present results highlight the role of transport from the ER in the initial events of the autophagic vacuole development.Fil: Zoppino, Felipe Carlos Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Militello, Rodrigo Damián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Slavin, Ileana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Alvarez, Cecilia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Colombo, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaWiley Blackwell Publishing, Inc2010-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/277457Zoppino, Felipe Carlos Martin; Militello, Rodrigo Damián; Slavin, Ileana; Alvarez, Cecilia Ines; Colombo, Maria Isabel; Autophagosome Formation Depends on the Small GTPase Rab1 and Functional ER Exit Sites; Wiley Blackwell Publishing, Inc; Traffic; 11; 9; 9-2010; 1246-12611398-9219CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/j.1600-0854.2010.01086.xinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1600-0854.2010.01086.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-23T14:29:47Zoai:ri.conicet.gov.ar:11336/277457instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-23 14:29:48.038CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Autophagosome Formation Depends on the Small GTPase Rab1 and Functional ER Exit Sites
title Autophagosome Formation Depends on the Small GTPase Rab1 and Functional ER Exit Sites
spellingShingle Autophagosome Formation Depends on the Small GTPase Rab1 and Functional ER Exit Sites
Zoppino, Felipe Carlos Martin
autophagy
Rab1
Sar1
endoplasmic reticulum
title_short Autophagosome Formation Depends on the Small GTPase Rab1 and Functional ER Exit Sites
title_full Autophagosome Formation Depends on the Small GTPase Rab1 and Functional ER Exit Sites
title_fullStr Autophagosome Formation Depends on the Small GTPase Rab1 and Functional ER Exit Sites
title_full_unstemmed Autophagosome Formation Depends on the Small GTPase Rab1 and Functional ER Exit Sites
title_sort Autophagosome Formation Depends on the Small GTPase Rab1 and Functional ER Exit Sites
dc.creator.none.fl_str_mv Zoppino, Felipe Carlos Martin
Militello, Rodrigo Damián
Slavin, Ileana
Alvarez, Cecilia Ines
Colombo, Maria Isabel
author Zoppino, Felipe Carlos Martin
author_facet Zoppino, Felipe Carlos Martin
Militello, Rodrigo Damián
Slavin, Ileana
Alvarez, Cecilia Ines
Colombo, Maria Isabel
author_role author
author2 Militello, Rodrigo Damián
Slavin, Ileana
Alvarez, Cecilia Ines
Colombo, Maria Isabel
author2_role author
author
author
author
dc.subject.none.fl_str_mv autophagy
Rab1
Sar1
endoplasmic reticulum
topic autophagy
Rab1
Sar1
endoplasmic reticulum
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Autophagy is an important cellular degradation pathway present in all eukaryotic cells. Via this pathway, portions of the cytoplasm and/or organelles are sequestered in double membrane structures called autophagosomes. In spite of the significant advance achieved in autophagy, the long-standing question about the source of the autophagic membrane remains unsolved. We have investigated the role of the secretory pathway in autophagosome biogenesis. Sar1 and Rab1b are monomeric GTPases that control traffic from the ER to the Golgi. We present evidence indicating that the activity of both proteins is required for autophagosome formation. Overexpression of dominant negative mutants and the use of siRNAs impaired autophagosome generation as determined by LC3- puncta formation and LC3-II processing. In addition our results indicate that the autophagic and secretory pathways intersect at a level preceding the brefeldin A blockage, suggesting that transport from the cis/medial Golgi is not necessary for autophagosome biogenesis. Our present results highlight the role of transport from the ER in the initial events of the autophagic vacuole development.
Fil: Zoppino, Felipe Carlos Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Militello, Rodrigo Damián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Slavin, Ileana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Alvarez, Cecilia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Colombo, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
description Autophagy is an important cellular degradation pathway present in all eukaryotic cells. Via this pathway, portions of the cytoplasm and/or organelles are sequestered in double membrane structures called autophagosomes. In spite of the significant advance achieved in autophagy, the long-standing question about the source of the autophagic membrane remains unsolved. We have investigated the role of the secretory pathway in autophagosome biogenesis. Sar1 and Rab1b are monomeric GTPases that control traffic from the ER to the Golgi. We present evidence indicating that the activity of both proteins is required for autophagosome formation. Overexpression of dominant negative mutants and the use of siRNAs impaired autophagosome generation as determined by LC3- puncta formation and LC3-II processing. In addition our results indicate that the autophagic and secretory pathways intersect at a level preceding the brefeldin A blockage, suggesting that transport from the cis/medial Golgi is not necessary for autophagosome biogenesis. Our present results highlight the role of transport from the ER in the initial events of the autophagic vacuole development.
publishDate 2010
dc.date.none.fl_str_mv 2010-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/277457
Zoppino, Felipe Carlos Martin; Militello, Rodrigo Damián; Slavin, Ileana; Alvarez, Cecilia Ines; Colombo, Maria Isabel; Autophagosome Formation Depends on the Small GTPase Rab1 and Functional ER Exit Sites; Wiley Blackwell Publishing, Inc; Traffic; 11; 9; 9-2010; 1246-1261
1398-9219
CONICET Digital
CONICET
url http://hdl.handle.net/11336/277457
identifier_str_mv Zoppino, Felipe Carlos Martin; Militello, Rodrigo Damián; Slavin, Ileana; Alvarez, Cecilia Ines; Colombo, Maria Isabel; Autophagosome Formation Depends on the Small GTPase Rab1 and Functional ER Exit Sites; Wiley Blackwell Publishing, Inc; Traffic; 11; 9; 9-2010; 1246-1261
1398-9219
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/j.1600-0854.2010.01086.x
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1600-0854.2010.01086.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1852335689716203520
score 12.952241

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