Nuclear matrix protein Matrin 3 regulates alterna tive splicing and forms overlapping regulatory networks with PTB

Autores
Coelho, Miguel B.; Attig, Jan; Bellora, Nicolás; König, Julian; Hallegger, Martina; Kayikci, Melis; Eyras, Eduardo; Ule, Jernej; Smith, Christopher W. J.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Matrin3 is an RNA‐ and DNA‐binding nuclear matrix protein found to be associated with neural and muscular degenerative diseases. A number of possible functions of Matrin3 have been suggested, but no widespread role in RNA metabolism has yet been clearly demonstrated. We identified Matrin3 by its interaction with the second RRM domain of the splicing regulator PTB. Using a combination of RNAi knockdown, transcriptome profiling and iCLIP, we find that Matrin3 is a regulator of hundreds of alternative splicing events, principally acting as a splicing repressor with only a small proportion of targeted events being co‐regulated by PTB. In contrast to other splicing regulators, Matrin3 binds to an extended region within repressed exons and flanking introns with no sharply defined peaks. The identification of this clear molecular function of Matrin3 should help to clarify the molecular pathology of ALS and other diseases caused by mutations of Matrin3.
Fil: Coelho, Miguel B.. University of Cambridge; Reino Unido
Fil: Attig, Jan. UCL Institute of Neurology; Reino Unido. MRC-Laboratory of Molecular Biology; Reino Unido
Fil: Bellora, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación En Biodiversidad y Medioambiente; Argentina. Universitat Pompeu Fabra; España. Institució Catalana de Recerca i Estudis Avancats; España. Universidad Nacional del Comahue; Argentina
Fil: König, Julian. MRC-Laboratory of Molecular Biology; Reino Unido
Fil: Hallegger, Martina. University of Cambridge; Reino Unido. UCL Institute of Neurology; Reino Unido
Fil: Kayikci, Melis. MRC-Laboratory of Molecular Biology; Reino Unido
Fil: Eyras, Eduardo. Universitat Pompeu Fabra; España. Institució Catalana de Recerca i Estudis Avancats; España
Fil: Ule, Jernej. UCL Institute of Neurology; Reino Unido
Fil: Smith, Christopher W. J.. University of Cambridge; Reino Unido
Materia
Alternative Splicing
Matrin3
Ptb
Regulatory Motifs
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/11980
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/11980
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network_name_str CONICET Digital (CONICET)
spelling Nuclear matrix protein Matrin 3 regulates alterna tive splicing and forms overlapping regulatory networks with PTBCoelho, Miguel B.Attig, JanBellora, NicolásKönig, JulianHallegger, MartinaKayikci, MelisEyras, EduardoUle, JernejSmith, Christopher W. J.Alternative SplicingMatrin3PtbRegulatory Motifshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Matrin3 is an RNA‐ and DNA‐binding nuclear matrix protein found to be associated with neural and muscular degenerative diseases. A number of possible functions of Matrin3 have been suggested, but no widespread role in RNA metabolism has yet been clearly demonstrated. We identified Matrin3 by its interaction with the second RRM domain of the splicing regulator PTB. Using a combination of RNAi knockdown, transcriptome profiling and iCLIP, we find that Matrin3 is a regulator of hundreds of alternative splicing events, principally acting as a splicing repressor with only a small proportion of targeted events being co‐regulated by PTB. In contrast to other splicing regulators, Matrin3 binds to an extended region within repressed exons and flanking introns with no sharply defined peaks. The identification of this clear molecular function of Matrin3 should help to clarify the molecular pathology of ALS and other diseases caused by mutations of Matrin3.Fil: Coelho, Miguel B.. University of Cambridge; Reino UnidoFil: Attig, Jan. UCL Institute of Neurology; Reino Unido. MRC-Laboratory of Molecular Biology; Reino UnidoFil: Bellora, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación En Biodiversidad y Medioambiente; Argentina. Universitat Pompeu Fabra; España. Institució Catalana de Recerca i Estudis Avancats; España. Universidad Nacional del Comahue; ArgentinaFil: König, Julian. MRC-Laboratory of Molecular Biology; Reino UnidoFil: Hallegger, Martina. University of Cambridge; Reino Unido. UCL Institute of Neurology; Reino UnidoFil: Kayikci, Melis. MRC-Laboratory of Molecular Biology; Reino UnidoFil: Eyras, Eduardo. Universitat Pompeu Fabra; España. Institució Catalana de Recerca i Estudis Avancats; EspañaFil: Ule, Jernej. UCL Institute of Neurology; Reino UnidoFil: Smith, Christopher W. J.. University of Cambridge; Reino UnidoNature Publishing Group2015-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/11980Coelho, Miguel B.; Attig, Jan; Bellora, Nicolás; König, Julian; Hallegger, Martina; et al.; Nuclear matrix protein Matrin 3 regulates alterna tive splicing and forms overlapping regulatory networks with PTB; Nature Publishing Group; Embo Journal; 34; 5; 1-2015; 653-6680261-4189enginfo:eu-repo/semantics/altIdentifier/url/http://emboj.embopress.org/content/34/5/653info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4365034/info:eu-repo/semantics/altIdentifier/doi/10.15252/embj.201489852info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:55:50Zoai:ri.conicet.gov.ar:11336/11980instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:55:50.86CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Nuclear matrix protein Matrin 3 regulates alterna tive splicing and forms overlapping regulatory networks with PTB
title Nuclear matrix protein Matrin 3 regulates alterna tive splicing and forms overlapping regulatory networks with PTB
spellingShingle Nuclear matrix protein Matrin 3 regulates alterna tive splicing and forms overlapping regulatory networks with PTB
Coelho, Miguel B.
Alternative Splicing
Matrin3
Ptb
Regulatory Motifs
title_short Nuclear matrix protein Matrin 3 regulates alterna tive splicing and forms overlapping regulatory networks with PTB
title_full Nuclear matrix protein Matrin 3 regulates alterna tive splicing and forms overlapping regulatory networks with PTB
title_fullStr Nuclear matrix protein Matrin 3 regulates alterna tive splicing and forms overlapping regulatory networks with PTB
title_full_unstemmed Nuclear matrix protein Matrin 3 regulates alterna tive splicing and forms overlapping regulatory networks with PTB
title_sort Nuclear matrix protein Matrin 3 regulates alterna tive splicing and forms overlapping regulatory networks with PTB
dc.creator.none.fl_str_mv Coelho, Miguel B.
Attig, Jan
Bellora, Nicolás
König, Julian
Hallegger, Martina
Kayikci, Melis
Eyras, Eduardo
Ule, Jernej
Smith, Christopher W. J.
author Coelho, Miguel B.
author_facet Coelho, Miguel B.
Attig, Jan
Bellora, Nicolás
König, Julian
Hallegger, Martina
Kayikci, Melis
Eyras, Eduardo
Ule, Jernej
Smith, Christopher W. J.
author_role author
author2 Attig, Jan
Bellora, Nicolás
König, Julian
Hallegger, Martina
Kayikci, Melis
Eyras, Eduardo
Ule, Jernej
Smith, Christopher W. J.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Alternative Splicing
Matrin3
Ptb
Regulatory Motifs
topic Alternative Splicing
Matrin3
Ptb
Regulatory Motifs
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Matrin3 is an RNA‐ and DNA‐binding nuclear matrix protein found to be associated with neural and muscular degenerative diseases. A number of possible functions of Matrin3 have been suggested, but no widespread role in RNA metabolism has yet been clearly demonstrated. We identified Matrin3 by its interaction with the second RRM domain of the splicing regulator PTB. Using a combination of RNAi knockdown, transcriptome profiling and iCLIP, we find that Matrin3 is a regulator of hundreds of alternative splicing events, principally acting as a splicing repressor with only a small proportion of targeted events being co‐regulated by PTB. In contrast to other splicing regulators, Matrin3 binds to an extended region within repressed exons and flanking introns with no sharply defined peaks. The identification of this clear molecular function of Matrin3 should help to clarify the molecular pathology of ALS and other diseases caused by mutations of Matrin3.
Fil: Coelho, Miguel B.. University of Cambridge; Reino Unido
Fil: Attig, Jan. UCL Institute of Neurology; Reino Unido. MRC-Laboratory of Molecular Biology; Reino Unido
Fil: Bellora, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación En Biodiversidad y Medioambiente; Argentina. Universitat Pompeu Fabra; España. Institució Catalana de Recerca i Estudis Avancats; España. Universidad Nacional del Comahue; Argentina
Fil: König, Julian. MRC-Laboratory of Molecular Biology; Reino Unido
Fil: Hallegger, Martina. University of Cambridge; Reino Unido. UCL Institute of Neurology; Reino Unido
Fil: Kayikci, Melis. MRC-Laboratory of Molecular Biology; Reino Unido
Fil: Eyras, Eduardo. Universitat Pompeu Fabra; España. Institució Catalana de Recerca i Estudis Avancats; España
Fil: Ule, Jernej. UCL Institute of Neurology; Reino Unido
Fil: Smith, Christopher W. J.. University of Cambridge; Reino Unido
description Matrin3 is an RNA‐ and DNA‐binding nuclear matrix protein found to be associated with neural and muscular degenerative diseases. A number of possible functions of Matrin3 have been suggested, but no widespread role in RNA metabolism has yet been clearly demonstrated. We identified Matrin3 by its interaction with the second RRM domain of the splicing regulator PTB. Using a combination of RNAi knockdown, transcriptome profiling and iCLIP, we find that Matrin3 is a regulator of hundreds of alternative splicing events, principally acting as a splicing repressor with only a small proportion of targeted events being co‐regulated by PTB. In contrast to other splicing regulators, Matrin3 binds to an extended region within repressed exons and flanking introns with no sharply defined peaks. The identification of this clear molecular function of Matrin3 should help to clarify the molecular pathology of ALS and other diseases caused by mutations of Matrin3.
publishDate 2015
dc.date.none.fl_str_mv 2015-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/11980
Coelho, Miguel B.; Attig, Jan; Bellora, Nicolás; König, Julian; Hallegger, Martina; et al.; Nuclear matrix protein Matrin 3 regulates alterna tive splicing and forms overlapping regulatory networks with PTB; Nature Publishing Group; Embo Journal; 34; 5; 1-2015; 653-668
0261-4189
url http://hdl.handle.net/11336/11980
identifier_str_mv Coelho, Miguel B.; Attig, Jan; Bellora, Nicolás; König, Julian; Hallegger, Martina; et al.; Nuclear matrix protein Matrin 3 regulates alterna tive splicing and forms overlapping regulatory networks with PTB; Nature Publishing Group; Embo Journal; 34; 5; 1-2015; 653-668
0261-4189
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://emboj.embopress.org/content/34/5/653
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4365034/
info:eu-repo/semantics/altIdentifier/doi/10.15252/embj.201489852
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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