Solving the boundary artifact for the enhanced deconvolution algorithm suppose applied to fluorescence microscopy

Autores
Toscani, Micaela; Martinez, Sandra Rita
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The SUPPOSe enhanced deconvolution algorithm relies in assuming that the image source can be described by an incoherent superposition of virtual point sources of equal intensity and finding the number and position of such virtual sources. In this work we describe the recent advances in the implementation of the method to gain resolution and remove artifacts due to the presence of fluorescent molecules close enough to the image frame boundary. The method was modified re-moving the invariant used before given by the product of the flux of the virtual sources times the number of virtual sources, and replacing it by a new invariant given by the total flux within the frame, thus allowing the location of virtual sources outside the frame but contributing to the signal inside the frame.
Fil: Toscani, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Ingeniería Biomédica; Argentina
Fil: Martinez, Sandra Rita. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Investigaciones Matemáticas "Luis A. Santaló". Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Matemáticas "Luis A. Santaló"; Argentina
Materia
ARTIFACT
BOUNDARY
DECONVOLUTION
FLUORESCENCE
MICROSCOPY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/165124
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/165124
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Solving the boundary artifact for the enhanced deconvolution algorithm suppose applied to fluorescence microscopyToscani, MicaelaMartinez, Sandra RitaARTIFACTBOUNDARYDECONVOLUTIONFLUORESCENCEMICROSCOPYhttps://purl.org/becyt/ford/1.3https://purl.org/becyt/ford/1The SUPPOSe enhanced deconvolution algorithm relies in assuming that the image source can be described by an incoherent superposition of virtual point sources of equal intensity and finding the number and position of such virtual sources. In this work we describe the recent advances in the implementation of the method to gain resolution and remove artifacts due to the presence of fluorescent molecules close enough to the image frame boundary. The method was modified re-moving the invariant used before given by the product of the flux of the virtual sources times the number of virtual sources, and replacing it by a new invariant given by the total flux within the frame, thus allowing the location of virtual sources outside the frame but contributing to the signal inside the frame.Fil: Toscani, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Ingeniería Biomédica; ArgentinaFil: Martinez, Sandra Rita. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Investigaciones Matemáticas "Luis A. Santaló". Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Matemáticas "Luis A. Santaló"; ArgentinaInstitution of Russian Academy of Sciences2021-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/165124Toscani, Micaela; Martinez, Sandra Rita; Solving the boundary artifact for the enhanced deconvolution algorithm suppose applied to fluorescence microscopy; Institution of Russian Academy of Sciences; Computer Optics; 45; 3; 2-2021; 418-4260134-24522412-6179CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.18287/2412-6179-CO-825info:eu-repo/semantics/altIdentifier/url/http://computeroptics.ru/eng/KO/Annot/KO45-3/450314e.htmlinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:25:43Zoai:ri.conicet.gov.ar:11336/165124instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:25:43.481CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Solving the boundary artifact for the enhanced deconvolution algorithm suppose applied to fluorescence microscopy
title Solving the boundary artifact for the enhanced deconvolution algorithm suppose applied to fluorescence microscopy
spellingShingle Solving the boundary artifact for the enhanced deconvolution algorithm suppose applied to fluorescence microscopy
Toscani, Micaela
ARTIFACT
BOUNDARY
DECONVOLUTION
FLUORESCENCE
MICROSCOPY
title_short Solving the boundary artifact for the enhanced deconvolution algorithm suppose applied to fluorescence microscopy
title_full Solving the boundary artifact for the enhanced deconvolution algorithm suppose applied to fluorescence microscopy
title_fullStr Solving the boundary artifact for the enhanced deconvolution algorithm suppose applied to fluorescence microscopy
title_full_unstemmed Solving the boundary artifact for the enhanced deconvolution algorithm suppose applied to fluorescence microscopy
title_sort Solving the boundary artifact for the enhanced deconvolution algorithm suppose applied to fluorescence microscopy
dc.creator.none.fl_str_mv Toscani, Micaela
Martinez, Sandra Rita
author Toscani, Micaela
author_facet Toscani, Micaela
Martinez, Sandra Rita
author_role author
author2 Martinez, Sandra Rita
author2_role author
dc.subject.none.fl_str_mv ARTIFACT
BOUNDARY
DECONVOLUTION
FLUORESCENCE
MICROSCOPY
topic ARTIFACT
BOUNDARY
DECONVOLUTION
FLUORESCENCE
MICROSCOPY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.3
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The SUPPOSe enhanced deconvolution algorithm relies in assuming that the image source can be described by an incoherent superposition of virtual point sources of equal intensity and finding the number and position of such virtual sources. In this work we describe the recent advances in the implementation of the method to gain resolution and remove artifacts due to the presence of fluorescent molecules close enough to the image frame boundary. The method was modified re-moving the invariant used before given by the product of the flux of the virtual sources times the number of virtual sources, and replacing it by a new invariant given by the total flux within the frame, thus allowing the location of virtual sources outside the frame but contributing to the signal inside the frame.
Fil: Toscani, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Ingeniería Biomédica; Argentina
Fil: Martinez, Sandra Rita. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Investigaciones Matemáticas "Luis A. Santaló". Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Matemáticas "Luis A. Santaló"; Argentina
description The SUPPOSe enhanced deconvolution algorithm relies in assuming that the image source can be described by an incoherent superposition of virtual point sources of equal intensity and finding the number and position of such virtual sources. In this work we describe the recent advances in the implementation of the method to gain resolution and remove artifacts due to the presence of fluorescent molecules close enough to the image frame boundary. The method was modified re-moving the invariant used before given by the product of the flux of the virtual sources times the number of virtual sources, and replacing it by a new invariant given by the total flux within the frame, thus allowing the location of virtual sources outside the frame but contributing to the signal inside the frame.
publishDate 2021
dc.date.none.fl_str_mv 2021-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/165124
Toscani, Micaela; Martinez, Sandra Rita; Solving the boundary artifact for the enhanced deconvolution algorithm suppose applied to fluorescence microscopy; Institution of Russian Academy of Sciences; Computer Optics; 45; 3; 2-2021; 418-426
0134-2452
2412-6179
CONICET Digital
CONICET
url http://hdl.handle.net/11336/165124
identifier_str_mv Toscani, Micaela; Martinez, Sandra Rita; Solving the boundary artifact for the enhanced deconvolution algorithm suppose applied to fluorescence microscopy; Institution of Russian Academy of Sciences; Computer Optics; 45; 3; 2-2021; 418-426
0134-2452
2412-6179
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.18287/2412-6179-CO-825
info:eu-repo/semantics/altIdentifier/url/http://computeroptics.ru/eng/KO/Annot/KO45-3/450314e.html
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Institution of Russian Academy of Sciences
publisher.none.fl_str_mv Institution of Russian Academy of Sciences
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082694966935552
score 13.22299

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