Solving the boundary artifact for the enhanced deconvolution algorithm suppose applied to fluorescence microscopy
- Autores
- Toscani, Micaela; Martinez, Sandra Rita
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The SUPPOSe enhanced deconvolution algorithm relies in assuming that the image source can be described by an incoherent superposition of virtual point sources of equal intensity and finding the number and position of such virtual sources. In this work we describe the recent advances in the implementation of the method to gain resolution and remove artifacts due to the presence of fluorescent molecules close enough to the image frame boundary. The method was modified re-moving the invariant used before given by the product of the flux of the virtual sources times the number of virtual sources, and replacing it by a new invariant given by the total flux within the frame, thus allowing the location of virtual sources outside the frame but contributing to the signal inside the frame.
Fil: Toscani, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Ingeniería Biomédica; Argentina
Fil: Martinez, Sandra Rita. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Investigaciones Matemáticas "Luis A. Santaló". Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Matemáticas "Luis A. Santaló"; Argentina - Materia
-
ARTIFACT
BOUNDARY
DECONVOLUTION
FLUORESCENCE
MICROSCOPY - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/165124
Ver los metadatos del registro completo
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Solving the boundary artifact for the enhanced deconvolution algorithm suppose applied to fluorescence microscopyToscani, MicaelaMartinez, Sandra RitaARTIFACTBOUNDARYDECONVOLUTIONFLUORESCENCEMICROSCOPYhttps://purl.org/becyt/ford/1.3https://purl.org/becyt/ford/1The SUPPOSe enhanced deconvolution algorithm relies in assuming that the image source can be described by an incoherent superposition of virtual point sources of equal intensity and finding the number and position of such virtual sources. In this work we describe the recent advances in the implementation of the method to gain resolution and remove artifacts due to the presence of fluorescent molecules close enough to the image frame boundary. The method was modified re-moving the invariant used before given by the product of the flux of the virtual sources times the number of virtual sources, and replacing it by a new invariant given by the total flux within the frame, thus allowing the location of virtual sources outside the frame but contributing to the signal inside the frame.Fil: Toscani, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Ingeniería Biomédica; ArgentinaFil: Martinez, Sandra Rita. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Investigaciones Matemáticas "Luis A. Santaló". Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Matemáticas "Luis A. Santaló"; ArgentinaInstitution of Russian Academy of Sciences2021-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/165124Toscani, Micaela; Martinez, Sandra Rita; Solving the boundary artifact for the enhanced deconvolution algorithm suppose applied to fluorescence microscopy; Institution of Russian Academy of Sciences; Computer Optics; 45; 3; 2-2021; 418-4260134-24522412-6179CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.18287/2412-6179-CO-825info:eu-repo/semantics/altIdentifier/url/http://computeroptics.ru/eng/KO/Annot/KO45-3/450314e.htmlinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:25:43Zoai:ri.conicet.gov.ar:11336/165124instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:25:43.481CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Solving the boundary artifact for the enhanced deconvolution algorithm suppose applied to fluorescence microscopy |
title |
Solving the boundary artifact for the enhanced deconvolution algorithm suppose applied to fluorescence microscopy |
spellingShingle |
Solving the boundary artifact for the enhanced deconvolution algorithm suppose applied to fluorescence microscopy Toscani, Micaela ARTIFACT BOUNDARY DECONVOLUTION FLUORESCENCE MICROSCOPY |
title_short |
Solving the boundary artifact for the enhanced deconvolution algorithm suppose applied to fluorescence microscopy |
title_full |
Solving the boundary artifact for the enhanced deconvolution algorithm suppose applied to fluorescence microscopy |
title_fullStr |
Solving the boundary artifact for the enhanced deconvolution algorithm suppose applied to fluorescence microscopy |
title_full_unstemmed |
Solving the boundary artifact for the enhanced deconvolution algorithm suppose applied to fluorescence microscopy |
title_sort |
Solving the boundary artifact for the enhanced deconvolution algorithm suppose applied to fluorescence microscopy |
dc.creator.none.fl_str_mv |
Toscani, Micaela Martinez, Sandra Rita |
author |
Toscani, Micaela |
author_facet |
Toscani, Micaela Martinez, Sandra Rita |
author_role |
author |
author2 |
Martinez, Sandra Rita |
author2_role |
author |
dc.subject.none.fl_str_mv |
ARTIFACT BOUNDARY DECONVOLUTION FLUORESCENCE MICROSCOPY |
topic |
ARTIFACT BOUNDARY DECONVOLUTION FLUORESCENCE MICROSCOPY |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.3 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The SUPPOSe enhanced deconvolution algorithm relies in assuming that the image source can be described by an incoherent superposition of virtual point sources of equal intensity and finding the number and position of such virtual sources. In this work we describe the recent advances in the implementation of the method to gain resolution and remove artifacts due to the presence of fluorescent molecules close enough to the image frame boundary. The method was modified re-moving the invariant used before given by the product of the flux of the virtual sources times the number of virtual sources, and replacing it by a new invariant given by the total flux within the frame, thus allowing the location of virtual sources outside the frame but contributing to the signal inside the frame. Fil: Toscani, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Ingeniería Biomédica; Argentina Fil: Martinez, Sandra Rita. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Investigaciones Matemáticas "Luis A. Santaló". Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Matemáticas "Luis A. Santaló"; Argentina |
description |
The SUPPOSe enhanced deconvolution algorithm relies in assuming that the image source can be described by an incoherent superposition of virtual point sources of equal intensity and finding the number and position of such virtual sources. In this work we describe the recent advances in the implementation of the method to gain resolution and remove artifacts due to the presence of fluorescent molecules close enough to the image frame boundary. The method was modified re-moving the invariant used before given by the product of the flux of the virtual sources times the number of virtual sources, and replacing it by a new invariant given by the total flux within the frame, thus allowing the location of virtual sources outside the frame but contributing to the signal inside the frame. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-02 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/165124 Toscani, Micaela; Martinez, Sandra Rita; Solving the boundary artifact for the enhanced deconvolution algorithm suppose applied to fluorescence microscopy; Institution of Russian Academy of Sciences; Computer Optics; 45; 3; 2-2021; 418-426 0134-2452 2412-6179 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/165124 |
identifier_str_mv |
Toscani, Micaela; Martinez, Sandra Rita; Solving the boundary artifact for the enhanced deconvolution algorithm suppose applied to fluorescence microscopy; Institution of Russian Academy of Sciences; Computer Optics; 45; 3; 2-2021; 418-426 0134-2452 2412-6179 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.18287/2412-6179-CO-825 info:eu-repo/semantics/altIdentifier/url/http://computeroptics.ru/eng/KO/Annot/KO45-3/450314e.html |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Institution of Russian Academy of Sciences |
publisher.none.fl_str_mv |
Institution of Russian Academy of Sciences |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.22299 |