Vector competence of Argentine mosquitoes (Diptera: Culicidae) for West Nile virus (Flaviviridae: Flavivirus)

Autores
Micieli, Maria Victoria; Matacchiero, Amy C.; Muttis, Evangelina; Fonseca, Dina Madeira; Aliota, Matthew T.; Kramer, Laura D.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We examined the ability of Culex pipiens L. complex mosquitoes from Argentina to vector West Nile virus (WNV) to assess their role in the transmission of WNV in South America. Several egg rafts of Culex spp. were collected from different breeding sites in the suburbs of the city of La Plata, Argentina, and a subset of each progeny was scored with morphological and genetic species indicators. Surprisingly, we did not ÞndCx. pipiensform pipiens, but found evidence of genetic hybrids of Culex quinquefasciatus and Cx. pipiens f. molestus. We then used morphological traits to create two colonies predominantly composed of one of these two taxa, although some hybrids are likely to have been included in both. These colonies were used in vector competence studies using NY99 and WN02 genotype strains of WNV obtained in New York State. As controls, we also tested colonies of U.S. Cx. quinquefasciatus and Cx. pipiens f. molestus. Additional Culex larvae from three drainage ditches near the cities of La Plata and Berisso, Argentina, were identiÞed by morphological and high-resolution molecular markers (microsatellites) asCx. quinquefasciatus Say,Cx. pipiensform molestus, and hybrids. Results indicate that Argentinian Culex are competent but only moderately efÞcient vectors of WNV and are less susceptible to this virus than comparable U.S. mosquito strains. Studies of vertical transmission of NY99 virus by Cx. pipiens f. molestus hybrids from Argentina yielded a minimal Þlial infection rate of 1.19 from females feeding during their second and later bloodmeals.
Fil: Micieli, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Matacchiero, Amy C.. Wadsworth Center. State of New York Department of Health; Estados Unidos
Fil: Muttis, Evangelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Fonseca, Dina Madeira. Rutgers University; Estados Unidos
Fil: Aliota, Matthew T.. Wadsworth Center. State of New York Department of Health; Estados Unidos
Fil: Kramer, Laura D.. Wadsworth Center. State of New York Department of Health; Estados Unidos
Materia
Culicidae
West Nile virus
Arbovirus
Vector competence
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/101964
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/101964
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Vector competence of Argentine mosquitoes (Diptera: Culicidae) for West Nile virus (Flaviviridae: Flavivirus)Micieli, Maria VictoriaMatacchiero, Amy C.Muttis, EvangelinaFonseca, Dina MadeiraAliota, Matthew T.Kramer, Laura D.CulicidaeWest Nile virusArbovirusVector competencehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We examined the ability of Culex pipiens L. complex mosquitoes from Argentina to vector West Nile virus (WNV) to assess their role in the transmission of WNV in South America. Several egg rafts of Culex spp. were collected from different breeding sites in the suburbs of the city of La Plata, Argentina, and a subset of each progeny was scored with morphological and genetic species indicators. Surprisingly, we did not ÞndCx. pipiensform pipiens, but found evidence of genetic hybrids of Culex quinquefasciatus and Cx. pipiens f. molestus. We then used morphological traits to create two colonies predominantly composed of one of these two taxa, although some hybrids are likely to have been included in both. These colonies were used in vector competence studies using NY99 and WN02 genotype strains of WNV obtained in New York State. As controls, we also tested colonies of U.S. Cx. quinquefasciatus and Cx. pipiens f. molestus. Additional Culex larvae from three drainage ditches near the cities of La Plata and Berisso, Argentina, were identiÞed by morphological and high-resolution molecular markers (microsatellites) asCx. quinquefasciatus Say,Cx. pipiensform molestus, and hybrids. Results indicate that Argentinian Culex are competent but only moderately efÞcient vectors of WNV and are less susceptible to this virus than comparable U.S. mosquito strains. Studies of vertical transmission of NY99 virus by Cx. pipiens f. molestus hybrids from Argentina yielded a minimal Þlial infection rate of 1.19 from females feeding during their second and later bloodmeals.Fil: Micieli, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Matacchiero, Amy C.. Wadsworth Center. State of New York Department of Health; Estados UnidosFil: Muttis, Evangelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Fonseca, Dina Madeira. Rutgers University; Estados UnidosFil: Aliota, Matthew T.. Wadsworth Center. State of New York Department of Health; Estados UnidosFil: Kramer, Laura D.. Wadsworth Center. State of New York Department of Health; Estados UnidosEntomological Society of America2013-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/101964Micieli, Maria Victoria; Matacchiero, Amy C.; Muttis, Evangelina; Fonseca, Dina Madeira; Aliota, Matthew T.; et al.; Vector competence of Argentine mosquitoes (Diptera: Culicidae) for West Nile virus (Flaviviridae: Flavivirus); Entomological Society of America; Journal of Medical Entomology; 50; 4; 4-2013; 853-8620022-2585CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1603%2Fme12226info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3932752/info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jme/article-abstract/50/4/853/890927info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:30:36Zoai:ri.conicet.gov.ar:11336/101964instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:30:36.638CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Vector competence of Argentine mosquitoes (Diptera: Culicidae) for West Nile virus (Flaviviridae: Flavivirus)
title Vector competence of Argentine mosquitoes (Diptera: Culicidae) for West Nile virus (Flaviviridae: Flavivirus)
spellingShingle Vector competence of Argentine mosquitoes (Diptera: Culicidae) for West Nile virus (Flaviviridae: Flavivirus)
Micieli, Maria Victoria
Culicidae
West Nile virus
Arbovirus
Vector competence
title_short Vector competence of Argentine mosquitoes (Diptera: Culicidae) for West Nile virus (Flaviviridae: Flavivirus)
title_full Vector competence of Argentine mosquitoes (Diptera: Culicidae) for West Nile virus (Flaviviridae: Flavivirus)
title_fullStr Vector competence of Argentine mosquitoes (Diptera: Culicidae) for West Nile virus (Flaviviridae: Flavivirus)
title_full_unstemmed Vector competence of Argentine mosquitoes (Diptera: Culicidae) for West Nile virus (Flaviviridae: Flavivirus)
title_sort Vector competence of Argentine mosquitoes (Diptera: Culicidae) for West Nile virus (Flaviviridae: Flavivirus)
dc.creator.none.fl_str_mv Micieli, Maria Victoria
Matacchiero, Amy C.
Muttis, Evangelina
Fonseca, Dina Madeira
Aliota, Matthew T.
Kramer, Laura D.
author Micieli, Maria Victoria
author_facet Micieli, Maria Victoria
Matacchiero, Amy C.
Muttis, Evangelina
Fonseca, Dina Madeira
Aliota, Matthew T.
Kramer, Laura D.
author_role author
author2 Matacchiero, Amy C.
Muttis, Evangelina
Fonseca, Dina Madeira
Aliota, Matthew T.
Kramer, Laura D.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Culicidae
West Nile virus
Arbovirus
Vector competence
topic Culicidae
West Nile virus
Arbovirus
Vector competence
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We examined the ability of Culex pipiens L. complex mosquitoes from Argentina to vector West Nile virus (WNV) to assess their role in the transmission of WNV in South America. Several egg rafts of Culex spp. were collected from different breeding sites in the suburbs of the city of La Plata, Argentina, and a subset of each progeny was scored with morphological and genetic species indicators. Surprisingly, we did not ÞndCx. pipiensform pipiens, but found evidence of genetic hybrids of Culex quinquefasciatus and Cx. pipiens f. molestus. We then used morphological traits to create two colonies predominantly composed of one of these two taxa, although some hybrids are likely to have been included in both. These colonies were used in vector competence studies using NY99 and WN02 genotype strains of WNV obtained in New York State. As controls, we also tested colonies of U.S. Cx. quinquefasciatus and Cx. pipiens f. molestus. Additional Culex larvae from three drainage ditches near the cities of La Plata and Berisso, Argentina, were identiÞed by morphological and high-resolution molecular markers (microsatellites) asCx. quinquefasciatus Say,Cx. pipiensform molestus, and hybrids. Results indicate that Argentinian Culex are competent but only moderately efÞcient vectors of WNV and are less susceptible to this virus than comparable U.S. mosquito strains. Studies of vertical transmission of NY99 virus by Cx. pipiens f. molestus hybrids from Argentina yielded a minimal Þlial infection rate of 1.19 from females feeding during their second and later bloodmeals.
Fil: Micieli, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Matacchiero, Amy C.. Wadsworth Center. State of New York Department of Health; Estados Unidos
Fil: Muttis, Evangelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Fonseca, Dina Madeira. Rutgers University; Estados Unidos
Fil: Aliota, Matthew T.. Wadsworth Center. State of New York Department of Health; Estados Unidos
Fil: Kramer, Laura D.. Wadsworth Center. State of New York Department of Health; Estados Unidos
description We examined the ability of Culex pipiens L. complex mosquitoes from Argentina to vector West Nile virus (WNV) to assess their role in the transmission of WNV in South America. Several egg rafts of Culex spp. were collected from different breeding sites in the suburbs of the city of La Plata, Argentina, and a subset of each progeny was scored with morphological and genetic species indicators. Surprisingly, we did not ÞndCx. pipiensform pipiens, but found evidence of genetic hybrids of Culex quinquefasciatus and Cx. pipiens f. molestus. We then used morphological traits to create two colonies predominantly composed of one of these two taxa, although some hybrids are likely to have been included in both. These colonies were used in vector competence studies using NY99 and WN02 genotype strains of WNV obtained in New York State. As controls, we also tested colonies of U.S. Cx. quinquefasciatus and Cx. pipiens f. molestus. Additional Culex larvae from three drainage ditches near the cities of La Plata and Berisso, Argentina, were identiÞed by morphological and high-resolution molecular markers (microsatellites) asCx. quinquefasciatus Say,Cx. pipiensform molestus, and hybrids. Results indicate that Argentinian Culex are competent but only moderately efÞcient vectors of WNV and are less susceptible to this virus than comparable U.S. mosquito strains. Studies of vertical transmission of NY99 virus by Cx. pipiens f. molestus hybrids from Argentina yielded a minimal Þlial infection rate of 1.19 from females feeding during their second and later bloodmeals.
publishDate 2013
dc.date.none.fl_str_mv 2013-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/101964
Micieli, Maria Victoria; Matacchiero, Amy C.; Muttis, Evangelina; Fonseca, Dina Madeira; Aliota, Matthew T.; et al.; Vector competence of Argentine mosquitoes (Diptera: Culicidae) for West Nile virus (Flaviviridae: Flavivirus); Entomological Society of America; Journal of Medical Entomology; 50; 4; 4-2013; 853-862
0022-2585
CONICET Digital
CONICET
url http://hdl.handle.net/11336/101964
identifier_str_mv Micieli, Maria Victoria; Matacchiero, Amy C.; Muttis, Evangelina; Fonseca, Dina Madeira; Aliota, Matthew T.; et al.; Vector competence of Argentine mosquitoes (Diptera: Culicidae) for West Nile virus (Flaviviridae: Flavivirus); Entomological Society of America; Journal of Medical Entomology; 50; 4; 4-2013; 853-862
0022-2585
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1603%2Fme12226
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3932752/
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jme/article-abstract/50/4/853/890927
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Entomological Society of America
publisher.none.fl_str_mv Entomological Society of America
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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