Vector competence for West Nile virus and St. Louis encephalitis virus (flavivirus) of three tick species of the genus Amblyomma (Acari: Ixodidae)
- Autores
- Flores, Fernando Sebastián; Zanluca, Camila; Guglielmone, Alberto Alejandro; Duarte dos Santos, Claudia N.; Labruna, Marcelo B.; Diaz, Luis Adrian
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Many species of Amblyomma ticks are commonly found infesting wild birds in South America, where birds are important hosts for several arboviruses, such as West Nile virus (WNV) and St. Louis encephalitis virus (SLEV). In this study, WNV and SLEV transmission experiments were performed to evaluate the vector competence of three South American tick species: Amblyomma ovale, Amblyomma tigrinum, and Amblyomma tonelliae. Larval and nymphal ticks of each species were allowed to feed on chicks needle inoculated with WNV or SLEV. All three Amblyomma species acquired either WNV or SLEV through larval feeding, with infection rates varying from 3.1% to 100% for WNV and from 0% to 35.7% for SLEV in engorged larvae. Transstadial perpetuation of the viruses was demonstrated in the molted nymphs, with WNV infection rates varying from 0% to 33.7% and SLEV infection rates from 13.6% to 23.8%. Although nymphal ticks also acquired either virus through feeding, transstadial perpetuation to adult ticks was lower, with virus detection in only 3.2% of A. tigrinum and 11.5% of A. tonelliae unfed adult ticks. On the other hand, vector competence for nymphs (exposed to WNV or SLEV through larval feeding) and adult ticks (exposed to WNV or SLEV through larval or nymphal feeding) was null in all cases. Although our results indicate transstadial perpetuation of WNV or SLEV in the three tick species, the ticks were not competent to transmit these agents to susceptible hosts. The role of these ixodid tick species in the epidemiology of WNV and SLEV might be insignificant, even though at least A. ovale and A. tigrinum are frequent bird ticks in Latin America, so the virus could survive winter in the fed larvae. However, future studies are required to determine the implications that this could have, as well as analyze the vector competence of other common bird tick species in South America.
Fil: Flores, Fernando Sebastián. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina
Fil: Zanluca, Camila. Instituto Carlos Chagas, Curitiba;
Fil: Guglielmone, Alberto Alejandro. Instituto Nacional de Tecnología Agropecuaria Eea, Rafaela; Argentina
Fil: Duarte dos Santos, Claudia N.. Instituto Carlos Chagas, Curitiba;
Fil: Labruna, Marcelo B.. Universidade de Sao Paulo; Brasil
Fil: Diaz, Luis Adrian. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina. Universidad Nacional de Córdoba; Argentina - Materia
-
WEST NILE VIRUS
ST. LOUIS ENCEPHALITIS VIRUS
AMBLYOMMA TICKS
VECTOR COMPETENCE - Nivel de accesibilidad
- acceso embargado
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/94726
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3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Vector competence for West Nile virus and St. Louis encephalitis virus (flavivirus) of three tick species of the genus Amblyomma (Acari: Ixodidae)Flores, Fernando SebastiánZanluca, CamilaGuglielmone, Alberto AlejandroDuarte dos Santos, Claudia N.Labruna, Marcelo B.Diaz, Luis AdrianWEST NILE VIRUSST. LOUIS ENCEPHALITIS VIRUSAMBLYOMMA TICKSVECTOR COMPETENCEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Many species of Amblyomma ticks are commonly found infesting wild birds in South America, where birds are important hosts for several arboviruses, such as West Nile virus (WNV) and St. Louis encephalitis virus (SLEV). In this study, WNV and SLEV transmission experiments were performed to evaluate the vector competence of three South American tick species: Amblyomma ovale, Amblyomma tigrinum, and Amblyomma tonelliae. Larval and nymphal ticks of each species were allowed to feed on chicks needle inoculated with WNV or SLEV. All three Amblyomma species acquired either WNV or SLEV through larval feeding, with infection rates varying from 3.1% to 100% for WNV and from 0% to 35.7% for SLEV in engorged larvae. Transstadial perpetuation of the viruses was demonstrated in the molted nymphs, with WNV infection rates varying from 0% to 33.7% and SLEV infection rates from 13.6% to 23.8%. Although nymphal ticks also acquired either virus through feeding, transstadial perpetuation to adult ticks was lower, with virus detection in only 3.2% of A. tigrinum and 11.5% of A. tonelliae unfed adult ticks. On the other hand, vector competence for nymphs (exposed to WNV or SLEV through larval feeding) and adult ticks (exposed to WNV or SLEV through larval or nymphal feeding) was null in all cases. Although our results indicate transstadial perpetuation of WNV or SLEV in the three tick species, the ticks were not competent to transmit these agents to susceptible hosts. The role of these ixodid tick species in the epidemiology of WNV and SLEV might be insignificant, even though at least A. ovale and A. tigrinum are frequent bird ticks in Latin America, so the virus could survive winter in the fed larvae. However, future studies are required to determine the implications that this could have, as well as analyze the vector competence of other common bird tick species in South America.Fil: Flores, Fernando Sebastián. Universidad Nacional de Córdoba. Facultad de Medicina; ArgentinaFil: Zanluca, Camila. Instituto Carlos Chagas, Curitiba; Fil: Guglielmone, Alberto Alejandro. Instituto Nacional de Tecnología Agropecuaria Eea, Rafaela; ArgentinaFil: Duarte dos Santos, Claudia N.. Instituto Carlos Chagas, Curitiba; Fil: Labruna, Marcelo B.. Universidade de Sao Paulo; BrasilFil: Diaz, Luis Adrian. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina. Universidad Nacional de Córdoba; ArgentinaAmerican Society of Tropical Medicine and Hygiene2019-05info:eu-repo/date/embargoEnd/2020-05-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/94726Flores, Fernando Sebastián; Zanluca, Camila; Guglielmone, Alberto Alejandro; Duarte dos Santos, Claudia N.; Labruna, Marcelo B.; et al.; Vector competence for West Nile virus and St. Louis encephalitis virus (flavivirus) of three tick species of the genus Amblyomma (Acari: Ixodidae); American Society of Tropical Medicine and Hygiene; American Journal of Tropical Medicine and Hygiene; 100; 5; 5-2019; 1230-12350002-9637CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.ajtmh.org/content/journals/10.4269/ajtmh.18-0134info:eu-repo/semantics/altIdentifier/doi/10.4269/ajtmh.18-0134info:eu-repo/semantics/embargoedAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:55:33Zoai:ri.conicet.gov.ar:11336/94726instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:55:33.73CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Vector competence for West Nile virus and St. Louis encephalitis virus (flavivirus) of three tick species of the genus Amblyomma (Acari: Ixodidae) |
title |
Vector competence for West Nile virus and St. Louis encephalitis virus (flavivirus) of three tick species of the genus Amblyomma (Acari: Ixodidae) |
spellingShingle |
Vector competence for West Nile virus and St. Louis encephalitis virus (flavivirus) of three tick species of the genus Amblyomma (Acari: Ixodidae) Flores, Fernando Sebastián WEST NILE VIRUS ST. LOUIS ENCEPHALITIS VIRUS AMBLYOMMA TICKS VECTOR COMPETENCE |
title_short |
Vector competence for West Nile virus and St. Louis encephalitis virus (flavivirus) of three tick species of the genus Amblyomma (Acari: Ixodidae) |
title_full |
Vector competence for West Nile virus and St. Louis encephalitis virus (flavivirus) of three tick species of the genus Amblyomma (Acari: Ixodidae) |
title_fullStr |
Vector competence for West Nile virus and St. Louis encephalitis virus (flavivirus) of three tick species of the genus Amblyomma (Acari: Ixodidae) |
title_full_unstemmed |
Vector competence for West Nile virus and St. Louis encephalitis virus (flavivirus) of three tick species of the genus Amblyomma (Acari: Ixodidae) |
title_sort |
Vector competence for West Nile virus and St. Louis encephalitis virus (flavivirus) of three tick species of the genus Amblyomma (Acari: Ixodidae) |
dc.creator.none.fl_str_mv |
Flores, Fernando Sebastián Zanluca, Camila Guglielmone, Alberto Alejandro Duarte dos Santos, Claudia N. Labruna, Marcelo B. Diaz, Luis Adrian |
author |
Flores, Fernando Sebastián |
author_facet |
Flores, Fernando Sebastián Zanluca, Camila Guglielmone, Alberto Alejandro Duarte dos Santos, Claudia N. Labruna, Marcelo B. Diaz, Luis Adrian |
author_role |
author |
author2 |
Zanluca, Camila Guglielmone, Alberto Alejandro Duarte dos Santos, Claudia N. Labruna, Marcelo B. Diaz, Luis Adrian |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
WEST NILE VIRUS ST. LOUIS ENCEPHALITIS VIRUS AMBLYOMMA TICKS VECTOR COMPETENCE |
topic |
WEST NILE VIRUS ST. LOUIS ENCEPHALITIS VIRUS AMBLYOMMA TICKS VECTOR COMPETENCE |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Many species of Amblyomma ticks are commonly found infesting wild birds in South America, where birds are important hosts for several arboviruses, such as West Nile virus (WNV) and St. Louis encephalitis virus (SLEV). In this study, WNV and SLEV transmission experiments were performed to evaluate the vector competence of three South American tick species: Amblyomma ovale, Amblyomma tigrinum, and Amblyomma tonelliae. Larval and nymphal ticks of each species were allowed to feed on chicks needle inoculated with WNV or SLEV. All three Amblyomma species acquired either WNV or SLEV through larval feeding, with infection rates varying from 3.1% to 100% for WNV and from 0% to 35.7% for SLEV in engorged larvae. Transstadial perpetuation of the viruses was demonstrated in the molted nymphs, with WNV infection rates varying from 0% to 33.7% and SLEV infection rates from 13.6% to 23.8%. Although nymphal ticks also acquired either virus through feeding, transstadial perpetuation to adult ticks was lower, with virus detection in only 3.2% of A. tigrinum and 11.5% of A. tonelliae unfed adult ticks. On the other hand, vector competence for nymphs (exposed to WNV or SLEV through larval feeding) and adult ticks (exposed to WNV or SLEV through larval or nymphal feeding) was null in all cases. Although our results indicate transstadial perpetuation of WNV or SLEV in the three tick species, the ticks were not competent to transmit these agents to susceptible hosts. The role of these ixodid tick species in the epidemiology of WNV and SLEV might be insignificant, even though at least A. ovale and A. tigrinum are frequent bird ticks in Latin America, so the virus could survive winter in the fed larvae. However, future studies are required to determine the implications that this could have, as well as analyze the vector competence of other common bird tick species in South America. Fil: Flores, Fernando Sebastián. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina Fil: Zanluca, Camila. Instituto Carlos Chagas, Curitiba; Fil: Guglielmone, Alberto Alejandro. Instituto Nacional de Tecnología Agropecuaria Eea, Rafaela; Argentina Fil: Duarte dos Santos, Claudia N.. Instituto Carlos Chagas, Curitiba; Fil: Labruna, Marcelo B.. Universidade de Sao Paulo; Brasil Fil: Diaz, Luis Adrian. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina. Universidad Nacional de Córdoba; Argentina |
description |
Many species of Amblyomma ticks are commonly found infesting wild birds in South America, where birds are important hosts for several arboviruses, such as West Nile virus (WNV) and St. Louis encephalitis virus (SLEV). In this study, WNV and SLEV transmission experiments were performed to evaluate the vector competence of three South American tick species: Amblyomma ovale, Amblyomma tigrinum, and Amblyomma tonelliae. Larval and nymphal ticks of each species were allowed to feed on chicks needle inoculated with WNV or SLEV. All three Amblyomma species acquired either WNV or SLEV through larval feeding, with infection rates varying from 3.1% to 100% for WNV and from 0% to 35.7% for SLEV in engorged larvae. Transstadial perpetuation of the viruses was demonstrated in the molted nymphs, with WNV infection rates varying from 0% to 33.7% and SLEV infection rates from 13.6% to 23.8%. Although nymphal ticks also acquired either virus through feeding, transstadial perpetuation to adult ticks was lower, with virus detection in only 3.2% of A. tigrinum and 11.5% of A. tonelliae unfed adult ticks. On the other hand, vector competence for nymphs (exposed to WNV or SLEV through larval feeding) and adult ticks (exposed to WNV or SLEV through larval or nymphal feeding) was null in all cases. Although our results indicate transstadial perpetuation of WNV or SLEV in the three tick species, the ticks were not competent to transmit these agents to susceptible hosts. The role of these ixodid tick species in the epidemiology of WNV and SLEV might be insignificant, even though at least A. ovale and A. tigrinum are frequent bird ticks in Latin America, so the virus could survive winter in the fed larvae. However, future studies are required to determine the implications that this could have, as well as analyze the vector competence of other common bird tick species in South America. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-05 info:eu-repo/date/embargoEnd/2020-05-31 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/94726 Flores, Fernando Sebastián; Zanluca, Camila; Guglielmone, Alberto Alejandro; Duarte dos Santos, Claudia N.; Labruna, Marcelo B.; et al.; Vector competence for West Nile virus and St. Louis encephalitis virus (flavivirus) of three tick species of the genus Amblyomma (Acari: Ixodidae); American Society of Tropical Medicine and Hygiene; American Journal of Tropical Medicine and Hygiene; 100; 5; 5-2019; 1230-1235 0002-9637 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/94726 |
identifier_str_mv |
Flores, Fernando Sebastián; Zanluca, Camila; Guglielmone, Alberto Alejandro; Duarte dos Santos, Claudia N.; Labruna, Marcelo B.; et al.; Vector competence for West Nile virus and St. Louis encephalitis virus (flavivirus) of three tick species of the genus Amblyomma (Acari: Ixodidae); American Society of Tropical Medicine and Hygiene; American Journal of Tropical Medicine and Hygiene; 100; 5; 5-2019; 1230-1235 0002-9637 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.ajtmh.org/content/journals/10.4269/ajtmh.18-0134 info:eu-repo/semantics/altIdentifier/doi/10.4269/ajtmh.18-0134 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/embargoedAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
embargoedAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
American Society of Tropical Medicine and Hygiene |
publisher.none.fl_str_mv |
American Society of Tropical Medicine and Hygiene |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613674949410816 |
score |
13.070432 |