Vector competence for West Nile virus and St. Louis encephalitis virus (flavivirus) of three tick species of the genus Amblyomma (Acari: Ixodidae)

Autores
Flores, Fernando Sebastián; Zanluca, Camila; Guglielmone, Alberto Alejandro; Duarte dos Santos, Claudia N.; Labruna, Marcelo B.; Diaz, Luis Adrian
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Many species of Amblyomma ticks are commonly found infesting wild birds in South America, where birds are important hosts for several arboviruses, such as West Nile virus (WNV) and St. Louis encephalitis virus (SLEV). In this study, WNV and SLEV transmission experiments were performed to evaluate the vector competence of three South American tick species: Amblyomma ovale, Amblyomma tigrinum, and Amblyomma tonelliae. Larval and nymphal ticks of each species were allowed to feed on chicks needle inoculated with WNV or SLEV. All three Amblyomma species acquired either WNV or SLEV through larval feeding, with infection rates varying from 3.1% to 100% for WNV and from 0% to 35.7% for SLEV in engorged larvae. Transstadial perpetuation of the viruses was demonstrated in the molted nymphs, with WNV infection rates varying from 0% to 33.7% and SLEV infection rates from 13.6% to 23.8%. Although nymphal ticks also acquired either virus through feeding, transstadial perpetuation to adult ticks was lower, with virus detection in only 3.2% of A. tigrinum and 11.5% of A. tonelliae unfed adult ticks. On the other hand, vector competence for nymphs (exposed to WNV or SLEV through larval feeding) and adult ticks (exposed to WNV or SLEV through larval or nymphal feeding) was null in all cases. Although our results indicate transstadial perpetuation of WNV or SLEV in the three tick species, the ticks were not competent to transmit these agents to susceptible hosts. The role of these ixodid tick species in the epidemiology of WNV and SLEV might be insignificant, even though at least A. ovale and A. tigrinum are frequent bird ticks in Latin America, so the virus could survive winter in the fed larvae. However, future studies are required to determine the implications that this could have, as well as analyze the vector competence of other common bird tick species in South America.
Fil: Flores, Fernando Sebastián. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina
Fil: Zanluca, Camila. Instituto Carlos Chagas, Curitiba;
Fil: Guglielmone, Alberto Alejandro. Instituto Nacional de Tecnología Agropecuaria Eea, Rafaela; Argentina
Fil: Duarte dos Santos, Claudia N.. Instituto Carlos Chagas, Curitiba;
Fil: Labruna, Marcelo B.. Universidade de Sao Paulo; Brasil
Fil: Diaz, Luis Adrian. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina. Universidad Nacional de Córdoba; Argentina
Materia
WEST NILE VIRUS
ST. LOUIS ENCEPHALITIS VIRUS
AMBLYOMMA TICKS
VECTOR COMPETENCE
Nivel de accesibilidad
acceso embargado
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/94726

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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Vector competence for West Nile virus and St. Louis encephalitis virus (flavivirus) of three tick species of the genus Amblyomma (Acari: Ixodidae)Flores, Fernando SebastiánZanluca, CamilaGuglielmone, Alberto AlejandroDuarte dos Santos, Claudia N.Labruna, Marcelo B.Diaz, Luis AdrianWEST NILE VIRUSST. LOUIS ENCEPHALITIS VIRUSAMBLYOMMA TICKSVECTOR COMPETENCEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Many species of Amblyomma ticks are commonly found infesting wild birds in South America, where birds are important hosts for several arboviruses, such as West Nile virus (WNV) and St. Louis encephalitis virus (SLEV). In this study, WNV and SLEV transmission experiments were performed to evaluate the vector competence of three South American tick species: Amblyomma ovale, Amblyomma tigrinum, and Amblyomma tonelliae. Larval and nymphal ticks of each species were allowed to feed on chicks needle inoculated with WNV or SLEV. All three Amblyomma species acquired either WNV or SLEV through larval feeding, with infection rates varying from 3.1% to 100% for WNV and from 0% to 35.7% for SLEV in engorged larvae. Transstadial perpetuation of the viruses was demonstrated in the molted nymphs, with WNV infection rates varying from 0% to 33.7% and SLEV infection rates from 13.6% to 23.8%. Although nymphal ticks also acquired either virus through feeding, transstadial perpetuation to adult ticks was lower, with virus detection in only 3.2% of A. tigrinum and 11.5% of A. tonelliae unfed adult ticks. On the other hand, vector competence for nymphs (exposed to WNV or SLEV through larval feeding) and adult ticks (exposed to WNV or SLEV through larval or nymphal feeding) was null in all cases. Although our results indicate transstadial perpetuation of WNV or SLEV in the three tick species, the ticks were not competent to transmit these agents to susceptible hosts. The role of these ixodid tick species in the epidemiology of WNV and SLEV might be insignificant, even though at least A. ovale and A. tigrinum are frequent bird ticks in Latin America, so the virus could survive winter in the fed larvae. However, future studies are required to determine the implications that this could have, as well as analyze the vector competence of other common bird tick species in South America.Fil: Flores, Fernando Sebastián. Universidad Nacional de Córdoba. Facultad de Medicina; ArgentinaFil: Zanluca, Camila. Instituto Carlos Chagas, Curitiba; Fil: Guglielmone, Alberto Alejandro. Instituto Nacional de Tecnología Agropecuaria Eea, Rafaela; ArgentinaFil: Duarte dos Santos, Claudia N.. Instituto Carlos Chagas, Curitiba; Fil: Labruna, Marcelo B.. Universidade de Sao Paulo; BrasilFil: Diaz, Luis Adrian. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina. Universidad Nacional de Córdoba; ArgentinaAmerican Society of Tropical Medicine and Hygiene2019-05info:eu-repo/date/embargoEnd/2020-05-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/94726Flores, Fernando Sebastián; Zanluca, Camila; Guglielmone, Alberto Alejandro; Duarte dos Santos, Claudia N.; Labruna, Marcelo B.; et al.; Vector competence for West Nile virus and St. Louis encephalitis virus (flavivirus) of three tick species of the genus Amblyomma (Acari: Ixodidae); American Society of Tropical Medicine and Hygiene; American Journal of Tropical Medicine and Hygiene; 100; 5; 5-2019; 1230-12350002-9637CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.ajtmh.org/content/journals/10.4269/ajtmh.18-0134info:eu-repo/semantics/altIdentifier/doi/10.4269/ajtmh.18-0134info:eu-repo/semantics/embargoedAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:55:33Zoai:ri.conicet.gov.ar:11336/94726instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:55:33.73CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Vector competence for West Nile virus and St. Louis encephalitis virus (flavivirus) of three tick species of the genus Amblyomma (Acari: Ixodidae)
title Vector competence for West Nile virus and St. Louis encephalitis virus (flavivirus) of three tick species of the genus Amblyomma (Acari: Ixodidae)
spellingShingle Vector competence for West Nile virus and St. Louis encephalitis virus (flavivirus) of three tick species of the genus Amblyomma (Acari: Ixodidae)
Flores, Fernando Sebastián
WEST NILE VIRUS
ST. LOUIS ENCEPHALITIS VIRUS
AMBLYOMMA TICKS
VECTOR COMPETENCE
title_short Vector competence for West Nile virus and St. Louis encephalitis virus (flavivirus) of three tick species of the genus Amblyomma (Acari: Ixodidae)
title_full Vector competence for West Nile virus and St. Louis encephalitis virus (flavivirus) of three tick species of the genus Amblyomma (Acari: Ixodidae)
title_fullStr Vector competence for West Nile virus and St. Louis encephalitis virus (flavivirus) of three tick species of the genus Amblyomma (Acari: Ixodidae)
title_full_unstemmed Vector competence for West Nile virus and St. Louis encephalitis virus (flavivirus) of three tick species of the genus Amblyomma (Acari: Ixodidae)
title_sort Vector competence for West Nile virus and St. Louis encephalitis virus (flavivirus) of three tick species of the genus Amblyomma (Acari: Ixodidae)
dc.creator.none.fl_str_mv Flores, Fernando Sebastián
Zanluca, Camila
Guglielmone, Alberto Alejandro
Duarte dos Santos, Claudia N.
Labruna, Marcelo B.
Diaz, Luis Adrian
author Flores, Fernando Sebastián
author_facet Flores, Fernando Sebastián
Zanluca, Camila
Guglielmone, Alberto Alejandro
Duarte dos Santos, Claudia N.
Labruna, Marcelo B.
Diaz, Luis Adrian
author_role author
author2 Zanluca, Camila
Guglielmone, Alberto Alejandro
Duarte dos Santos, Claudia N.
Labruna, Marcelo B.
Diaz, Luis Adrian
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv WEST NILE VIRUS
ST. LOUIS ENCEPHALITIS VIRUS
AMBLYOMMA TICKS
VECTOR COMPETENCE
topic WEST NILE VIRUS
ST. LOUIS ENCEPHALITIS VIRUS
AMBLYOMMA TICKS
VECTOR COMPETENCE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Many species of Amblyomma ticks are commonly found infesting wild birds in South America, where birds are important hosts for several arboviruses, such as West Nile virus (WNV) and St. Louis encephalitis virus (SLEV). In this study, WNV and SLEV transmission experiments were performed to evaluate the vector competence of three South American tick species: Amblyomma ovale, Amblyomma tigrinum, and Amblyomma tonelliae. Larval and nymphal ticks of each species were allowed to feed on chicks needle inoculated with WNV or SLEV. All three Amblyomma species acquired either WNV or SLEV through larval feeding, with infection rates varying from 3.1% to 100% for WNV and from 0% to 35.7% for SLEV in engorged larvae. Transstadial perpetuation of the viruses was demonstrated in the molted nymphs, with WNV infection rates varying from 0% to 33.7% and SLEV infection rates from 13.6% to 23.8%. Although nymphal ticks also acquired either virus through feeding, transstadial perpetuation to adult ticks was lower, with virus detection in only 3.2% of A. tigrinum and 11.5% of A. tonelliae unfed adult ticks. On the other hand, vector competence for nymphs (exposed to WNV or SLEV through larval feeding) and adult ticks (exposed to WNV or SLEV through larval or nymphal feeding) was null in all cases. Although our results indicate transstadial perpetuation of WNV or SLEV in the three tick species, the ticks were not competent to transmit these agents to susceptible hosts. The role of these ixodid tick species in the epidemiology of WNV and SLEV might be insignificant, even though at least A. ovale and A. tigrinum are frequent bird ticks in Latin America, so the virus could survive winter in the fed larvae. However, future studies are required to determine the implications that this could have, as well as analyze the vector competence of other common bird tick species in South America.
Fil: Flores, Fernando Sebastián. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina
Fil: Zanluca, Camila. Instituto Carlos Chagas, Curitiba;
Fil: Guglielmone, Alberto Alejandro. Instituto Nacional de Tecnología Agropecuaria Eea, Rafaela; Argentina
Fil: Duarte dos Santos, Claudia N.. Instituto Carlos Chagas, Curitiba;
Fil: Labruna, Marcelo B.. Universidade de Sao Paulo; Brasil
Fil: Diaz, Luis Adrian. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina. Universidad Nacional de Córdoba; Argentina
description Many species of Amblyomma ticks are commonly found infesting wild birds in South America, where birds are important hosts for several arboviruses, such as West Nile virus (WNV) and St. Louis encephalitis virus (SLEV). In this study, WNV and SLEV transmission experiments were performed to evaluate the vector competence of three South American tick species: Amblyomma ovale, Amblyomma tigrinum, and Amblyomma tonelliae. Larval and nymphal ticks of each species were allowed to feed on chicks needle inoculated with WNV or SLEV. All three Amblyomma species acquired either WNV or SLEV through larval feeding, with infection rates varying from 3.1% to 100% for WNV and from 0% to 35.7% for SLEV in engorged larvae. Transstadial perpetuation of the viruses was demonstrated in the molted nymphs, with WNV infection rates varying from 0% to 33.7% and SLEV infection rates from 13.6% to 23.8%. Although nymphal ticks also acquired either virus through feeding, transstadial perpetuation to adult ticks was lower, with virus detection in only 3.2% of A. tigrinum and 11.5% of A. tonelliae unfed adult ticks. On the other hand, vector competence for nymphs (exposed to WNV or SLEV through larval feeding) and adult ticks (exposed to WNV or SLEV through larval or nymphal feeding) was null in all cases. Although our results indicate transstadial perpetuation of WNV or SLEV in the three tick species, the ticks were not competent to transmit these agents to susceptible hosts. The role of these ixodid tick species in the epidemiology of WNV and SLEV might be insignificant, even though at least A. ovale and A. tigrinum are frequent bird ticks in Latin America, so the virus could survive winter in the fed larvae. However, future studies are required to determine the implications that this could have, as well as analyze the vector competence of other common bird tick species in South America.
publishDate 2019
dc.date.none.fl_str_mv 2019-05
info:eu-repo/date/embargoEnd/2020-05-31
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/94726
Flores, Fernando Sebastián; Zanluca, Camila; Guglielmone, Alberto Alejandro; Duarte dos Santos, Claudia N.; Labruna, Marcelo B.; et al.; Vector competence for West Nile virus and St. Louis encephalitis virus (flavivirus) of three tick species of the genus Amblyomma (Acari: Ixodidae); American Society of Tropical Medicine and Hygiene; American Journal of Tropical Medicine and Hygiene; 100; 5; 5-2019; 1230-1235
0002-9637
CONICET Digital
CONICET
url http://hdl.handle.net/11336/94726
identifier_str_mv Flores, Fernando Sebastián; Zanluca, Camila; Guglielmone, Alberto Alejandro; Duarte dos Santos, Claudia N.; Labruna, Marcelo B.; et al.; Vector competence for West Nile virus and St. Louis encephalitis virus (flavivirus) of three tick species of the genus Amblyomma (Acari: Ixodidae); American Society of Tropical Medicine and Hygiene; American Journal of Tropical Medicine and Hygiene; 100; 5; 5-2019; 1230-1235
0002-9637
CONICET Digital
CONICET
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language eng
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info:eu-repo/semantics/altIdentifier/doi/10.4269/ajtmh.18-0134
dc.rights.none.fl_str_mv info:eu-repo/semantics/embargoedAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv embargoedAccess
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dc.publisher.none.fl_str_mv American Society of Tropical Medicine and Hygiene
publisher.none.fl_str_mv American Society of Tropical Medicine and Hygiene
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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