Implication of Sphingosine-1-Phosphate Receptor 2 (S1PR2) in Differentiation and Dedifferentiation of Epithelial Renal Cells

Autores
Romero, Daniela Judith; Santacreu, Bruno Jaime; Tarallo, Estefania; Favale, Nicolas Octavio
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Epithelial cell differentiation is a process that involves the mesenchymal-epithelial transition (MET) and includes cell cycle arrest, cell-cell junction maturation in addition to changes in cell migration capacity. The epithelial-mesenchymal transition (EMT) is a dynamic process by which fully differentiated epithelial cells can acquire a mesenchymal phenotype. During EMT, cell adhesion and apical-basal polarity are lost, and the cytoskeleton is reorganized. Previous results from our laboratory showed that in Madin-Darby canine kidney cells (MDCK) under different culture conditions can achieve different stages of differentiation resembling MET. Sphingosine 1-phosphate (S1P) is a bioactive sphingolipid, produced by the phosphorylation of sphingosine by sphingosine kinases (SKs), which is involved in different processes such as proliferation, cell growth, differentiation, and migration. S1P can act both intracellularly as a second messenger or extracellularly as a ligand of 5 different G protein-coupled receptors (S1PR1-5). In the present work, we evaluated the importance of S1P acting on S1PR2 in the modulation of MET and EMT. We found that there are differences in the action of S1PR2 in MDCK cells that depends on the differentiation stage. S1PR2 positively modulates the passage from polarized to differentiated cells through MET. Inhibition of S1PR2 blocks adherens junction establishment, as well as apical and basal polarity. On the other hand, once cells have acquired the differentiated phenotype, S1PR2 induces the dedifferentiation of epithelial cells through EMT. Inhibition of S1PR2 triggers changes in EMT markers, such as rearrangements of the actin cytoskeleton, expression of vimentin, and nuclear translocation of beta-catenin, as well as Slug. The expression levels of S1PR2 in the different stages of differentiation of MDCK cells did not show significant differences. Instead, immunofluorescence studies showed that during cell differentiation, S1PR2 was progressively enriched at the plasma membrane. These results suggest that the location of S1PR2 depends on the stage of cell differentiation, and this determines its role. These findings highlight the great versatility of S1P on the control of physiological and pathophysiological processes.
Fil: Romero, Daniela Judith. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Santacreu, Bruno Jaime. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Tarallo, Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Favale, Nicolas Octavio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology; XV Annual Meeting Argentinean Society for General Microbiology
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Materia
EPITHELIAL-MESENCHYMAL TRANSITION
SPHINGOSINE-1-PHOSPHATE RECEPTOR 2
SPHINGOSINE 1-PHOSPHATE
EPITHELIAL RENAL CELLS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/173528
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Implication of Sphingosine-1-Phosphate Receptor 2 (S1PR2) in Differentiation and Dedifferentiation of Epithelial Renal CellsRomero, Daniela JudithSantacreu, Bruno JaimeTarallo, EstefaniaFavale, Nicolas OctavioEPITHELIAL-MESENCHYMAL TRANSITIONSPHINGOSINE-1-PHOSPHATE RECEPTOR 2SPHINGOSINE 1-PHOSPHATEEPITHELIAL RENAL CELLShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Epithelial cell differentiation is a process that involves the mesenchymal-epithelial transition (MET) and includes cell cycle arrest, cell-cell junction maturation in addition to changes in cell migration capacity. The epithelial-mesenchymal transition (EMT) is a dynamic process by which fully differentiated epithelial cells can acquire a mesenchymal phenotype. During EMT, cell adhesion and apical-basal polarity are lost, and the cytoskeleton is reorganized. Previous results from our laboratory showed that in Madin-Darby canine kidney cells (MDCK) under different culture conditions can achieve different stages of differentiation resembling MET. Sphingosine 1-phosphate (S1P) is a bioactive sphingolipid, produced by the phosphorylation of sphingosine by sphingosine kinases (SKs), which is involved in different processes such as proliferation, cell growth, differentiation, and migration. S1P can act both intracellularly as a second messenger or extracellularly as a ligand of 5 different G protein-coupled receptors (S1PR1-5). In the present work, we evaluated the importance of S1P acting on S1PR2 in the modulation of MET and EMT. We found that there are differences in the action of S1PR2 in MDCK cells that depends on the differentiation stage. S1PR2 positively modulates the passage from polarized to differentiated cells through MET. Inhibition of S1PR2 blocks adherens junction establishment, as well as apical and basal polarity. On the other hand, once cells have acquired the differentiated phenotype, S1PR2 induces the dedifferentiation of epithelial cells through EMT. Inhibition of S1PR2 triggers changes in EMT markers, such as rearrangements of the actin cytoskeleton, expression of vimentin, and nuclear translocation of beta-catenin, as well as Slug. The expression levels of S1PR2 in the different stages of differentiation of MDCK cells did not show significant differences. Instead, immunofluorescence studies showed that during cell differentiation, S1PR2 was progressively enriched at the plasma membrane. These results suggest that the location of S1PR2 depends on the stage of cell differentiation, and this determines its role. These findings highlight the great versatility of S1P on the control of physiological and pathophysiological processes.Fil: Romero, Daniela Judith. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Santacreu, Bruno Jaime. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Tarallo, Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Favale, Nicolas Octavio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaLVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology; XV Annual Meeting Argentinean Society for General MicrobiologyArgentinaSociedad Argentina de Investigación Bioquímica y Biología MolecularTech Science Press2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/173528Implication of Sphingosine-1-Phosphate Receptor 2 (S1PR2) in Differentiation and Dedifferentiation of Epithelial Renal Cells; LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology; XV Annual Meeting Argentinean Society for General Microbiology; Argentina; 2020; 1-10327-9545CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.samige.org.ar/admin/news/files/170-TSP_BIOCELL_42376.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:23:03Zoai:ri.conicet.gov.ar:11336/173528instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:23:03.677CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Implication of Sphingosine-1-Phosphate Receptor 2 (S1PR2) in Differentiation and Dedifferentiation of Epithelial Renal Cells
title Implication of Sphingosine-1-Phosphate Receptor 2 (S1PR2) in Differentiation and Dedifferentiation of Epithelial Renal Cells
spellingShingle Implication of Sphingosine-1-Phosphate Receptor 2 (S1PR2) in Differentiation and Dedifferentiation of Epithelial Renal Cells
Romero, Daniela Judith
EPITHELIAL-MESENCHYMAL TRANSITION
SPHINGOSINE-1-PHOSPHATE RECEPTOR 2
SPHINGOSINE 1-PHOSPHATE
EPITHELIAL RENAL CELLS
title_short Implication of Sphingosine-1-Phosphate Receptor 2 (S1PR2) in Differentiation and Dedifferentiation of Epithelial Renal Cells
title_full Implication of Sphingosine-1-Phosphate Receptor 2 (S1PR2) in Differentiation and Dedifferentiation of Epithelial Renal Cells
title_fullStr Implication of Sphingosine-1-Phosphate Receptor 2 (S1PR2) in Differentiation and Dedifferentiation of Epithelial Renal Cells
title_full_unstemmed Implication of Sphingosine-1-Phosphate Receptor 2 (S1PR2) in Differentiation and Dedifferentiation of Epithelial Renal Cells
title_sort Implication of Sphingosine-1-Phosphate Receptor 2 (S1PR2) in Differentiation and Dedifferentiation of Epithelial Renal Cells
dc.creator.none.fl_str_mv Romero, Daniela Judith
Santacreu, Bruno Jaime
Tarallo, Estefania
Favale, Nicolas Octavio
author Romero, Daniela Judith
author_facet Romero, Daniela Judith
Santacreu, Bruno Jaime
Tarallo, Estefania
Favale, Nicolas Octavio
author_role author
author2 Santacreu, Bruno Jaime
Tarallo, Estefania
Favale, Nicolas Octavio
author2_role author
author
author
dc.subject.none.fl_str_mv EPITHELIAL-MESENCHYMAL TRANSITION
SPHINGOSINE-1-PHOSPHATE RECEPTOR 2
SPHINGOSINE 1-PHOSPHATE
EPITHELIAL RENAL CELLS
topic EPITHELIAL-MESENCHYMAL TRANSITION
SPHINGOSINE-1-PHOSPHATE RECEPTOR 2
SPHINGOSINE 1-PHOSPHATE
EPITHELIAL RENAL CELLS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Epithelial cell differentiation is a process that involves the mesenchymal-epithelial transition (MET) and includes cell cycle arrest, cell-cell junction maturation in addition to changes in cell migration capacity. The epithelial-mesenchymal transition (EMT) is a dynamic process by which fully differentiated epithelial cells can acquire a mesenchymal phenotype. During EMT, cell adhesion and apical-basal polarity are lost, and the cytoskeleton is reorganized. Previous results from our laboratory showed that in Madin-Darby canine kidney cells (MDCK) under different culture conditions can achieve different stages of differentiation resembling MET. Sphingosine 1-phosphate (S1P) is a bioactive sphingolipid, produced by the phosphorylation of sphingosine by sphingosine kinases (SKs), which is involved in different processes such as proliferation, cell growth, differentiation, and migration. S1P can act both intracellularly as a second messenger or extracellularly as a ligand of 5 different G protein-coupled receptors (S1PR1-5). In the present work, we evaluated the importance of S1P acting on S1PR2 in the modulation of MET and EMT. We found that there are differences in the action of S1PR2 in MDCK cells that depends on the differentiation stage. S1PR2 positively modulates the passage from polarized to differentiated cells through MET. Inhibition of S1PR2 blocks adherens junction establishment, as well as apical and basal polarity. On the other hand, once cells have acquired the differentiated phenotype, S1PR2 induces the dedifferentiation of epithelial cells through EMT. Inhibition of S1PR2 triggers changes in EMT markers, such as rearrangements of the actin cytoskeleton, expression of vimentin, and nuclear translocation of beta-catenin, as well as Slug. The expression levels of S1PR2 in the different stages of differentiation of MDCK cells did not show significant differences. Instead, immunofluorescence studies showed that during cell differentiation, S1PR2 was progressively enriched at the plasma membrane. These results suggest that the location of S1PR2 depends on the stage of cell differentiation, and this determines its role. These findings highlight the great versatility of S1P on the control of physiological and pathophysiological processes.
Fil: Romero, Daniela Judith. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Santacreu, Bruno Jaime. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Tarallo, Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Favale, Nicolas Octavio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology; XV Annual Meeting Argentinean Society for General Microbiology
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
description Epithelial cell differentiation is a process that involves the mesenchymal-epithelial transition (MET) and includes cell cycle arrest, cell-cell junction maturation in addition to changes in cell migration capacity. The epithelial-mesenchymal transition (EMT) is a dynamic process by which fully differentiated epithelial cells can acquire a mesenchymal phenotype. During EMT, cell adhesion and apical-basal polarity are lost, and the cytoskeleton is reorganized. Previous results from our laboratory showed that in Madin-Darby canine kidney cells (MDCK) under different culture conditions can achieve different stages of differentiation resembling MET. Sphingosine 1-phosphate (S1P) is a bioactive sphingolipid, produced by the phosphorylation of sphingosine by sphingosine kinases (SKs), which is involved in different processes such as proliferation, cell growth, differentiation, and migration. S1P can act both intracellularly as a second messenger or extracellularly as a ligand of 5 different G protein-coupled receptors (S1PR1-5). In the present work, we evaluated the importance of S1P acting on S1PR2 in the modulation of MET and EMT. We found that there are differences in the action of S1PR2 in MDCK cells that depends on the differentiation stage. S1PR2 positively modulates the passage from polarized to differentiated cells through MET. Inhibition of S1PR2 blocks adherens junction establishment, as well as apical and basal polarity. On the other hand, once cells have acquired the differentiated phenotype, S1PR2 induces the dedifferentiation of epithelial cells through EMT. Inhibition of S1PR2 triggers changes in EMT markers, such as rearrangements of the actin cytoskeleton, expression of vimentin, and nuclear translocation of beta-catenin, as well as Slug. The expression levels of S1PR2 in the different stages of differentiation of MDCK cells did not show significant differences. Instead, immunofluorescence studies showed that during cell differentiation, S1PR2 was progressively enriched at the plasma membrane. These results suggest that the location of S1PR2 depends on the stage of cell differentiation, and this determines its role. These findings highlight the great versatility of S1P on the control of physiological and pathophysiological processes.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/173528
Implication of Sphingosine-1-Phosphate Receptor 2 (S1PR2) in Differentiation and Dedifferentiation of Epithelial Renal Cells; LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology; XV Annual Meeting Argentinean Society for General Microbiology; Argentina; 2020; 1-1
0327-9545
CONICET Digital
CONICET
url http://hdl.handle.net/11336/173528
identifier_str_mv Implication of Sphingosine-1-Phosphate Receptor 2 (S1PR2) in Differentiation and Dedifferentiation of Epithelial Renal Cells; LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology; XV Annual Meeting Argentinean Society for General Microbiology; Argentina; 2020; 1-1
0327-9545
CONICET Digital
CONICET
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language eng
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dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
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