Implication of Sphingosine-1-Phosphate Receptor 2 (S1PR2) in Differentiation and Dedifferentiation of Epithelial Renal Cells
- Autores
- Romero, Daniela Judith; Santacreu, Bruno Jaime; Tarallo, Estefania; Favale, Nicolas Octavio
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Epithelial cell differentiation is a process that involves the mesenchymal-epithelial transition (MET) and includes cell cycle arrest, cell-cell junction maturation in addition to changes in cell migration capacity. The epithelial-mesenchymal transition (EMT) is a dynamic process by which fully differentiated epithelial cells can acquire a mesenchymal phenotype. During EMT, cell adhesion and apical-basal polarity are lost, and the cytoskeleton is reorganized. Previous results from our laboratory showed that in Madin-Darby canine kidney cells (MDCK) under different culture conditions can achieve different stages of differentiation resembling MET. Sphingosine 1-phosphate (S1P) is a bioactive sphingolipid, produced by the phosphorylation of sphingosine by sphingosine kinases (SKs), which is involved in different processes such as proliferation, cell growth, differentiation, and migration. S1P can act both intracellularly as a second messenger or extracellularly as a ligand of 5 different G protein-coupled receptors (S1PR1-5). In the present work, we evaluated the importance of S1P acting on S1PR2 in the modulation of MET and EMT. We found that there are differences in the action of S1PR2 in MDCK cells that depends on the differentiation stage. S1PR2 positively modulates the passage from polarized to differentiated cells through MET. Inhibition of S1PR2 blocks adherens junction establishment, as well as apical and basal polarity. On the other hand, once cells have acquired the differentiated phenotype, S1PR2 induces the dedifferentiation of epithelial cells through EMT. Inhibition of S1PR2 triggers changes in EMT markers, such as rearrangements of the actin cytoskeleton, expression of vimentin, and nuclear translocation of beta-catenin, as well as Slug. The expression levels of S1PR2 in the different stages of differentiation of MDCK cells did not show significant differences. Instead, immunofluorescence studies showed that during cell differentiation, S1PR2 was progressively enriched at the plasma membrane. These results suggest that the location of S1PR2 depends on the stage of cell differentiation, and this determines its role. These findings highlight the great versatility of S1P on the control of physiological and pathophysiological processes.
Fil: Romero, Daniela Judith. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Santacreu, Bruno Jaime. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Tarallo, Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Favale, Nicolas Octavio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology; XV Annual Meeting Argentinean Society for General Microbiology
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología Molecular - Materia
-
EPITHELIAL-MESENCHYMAL TRANSITION
SPHINGOSINE-1-PHOSPHATE RECEPTOR 2
SPHINGOSINE 1-PHOSPHATE
EPITHELIAL RENAL CELLS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/173528
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Implication of Sphingosine-1-Phosphate Receptor 2 (S1PR2) in Differentiation and Dedifferentiation of Epithelial Renal CellsRomero, Daniela JudithSantacreu, Bruno JaimeTarallo, EstefaniaFavale, Nicolas OctavioEPITHELIAL-MESENCHYMAL TRANSITIONSPHINGOSINE-1-PHOSPHATE RECEPTOR 2SPHINGOSINE 1-PHOSPHATEEPITHELIAL RENAL CELLShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Epithelial cell differentiation is a process that involves the mesenchymal-epithelial transition (MET) and includes cell cycle arrest, cell-cell junction maturation in addition to changes in cell migration capacity. The epithelial-mesenchymal transition (EMT) is a dynamic process by which fully differentiated epithelial cells can acquire a mesenchymal phenotype. During EMT, cell adhesion and apical-basal polarity are lost, and the cytoskeleton is reorganized. Previous results from our laboratory showed that in Madin-Darby canine kidney cells (MDCK) under different culture conditions can achieve different stages of differentiation resembling MET. Sphingosine 1-phosphate (S1P) is a bioactive sphingolipid, produced by the phosphorylation of sphingosine by sphingosine kinases (SKs), which is involved in different processes such as proliferation, cell growth, differentiation, and migration. S1P can act both intracellularly as a second messenger or extracellularly as a ligand of 5 different G protein-coupled receptors (S1PR1-5). In the present work, we evaluated the importance of S1P acting on S1PR2 in the modulation of MET and EMT. We found that there are differences in the action of S1PR2 in MDCK cells that depends on the differentiation stage. S1PR2 positively modulates the passage from polarized to differentiated cells through MET. Inhibition of S1PR2 blocks adherens junction establishment, as well as apical and basal polarity. On the other hand, once cells have acquired the differentiated phenotype, S1PR2 induces the dedifferentiation of epithelial cells through EMT. Inhibition of S1PR2 triggers changes in EMT markers, such as rearrangements of the actin cytoskeleton, expression of vimentin, and nuclear translocation of beta-catenin, as well as Slug. The expression levels of S1PR2 in the different stages of differentiation of MDCK cells did not show significant differences. Instead, immunofluorescence studies showed that during cell differentiation, S1PR2 was progressively enriched at the plasma membrane. These results suggest that the location of S1PR2 depends on the stage of cell differentiation, and this determines its role. These findings highlight the great versatility of S1P on the control of physiological and pathophysiological processes.Fil: Romero, Daniela Judith. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Santacreu, Bruno Jaime. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Tarallo, Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Favale, Nicolas Octavio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaLVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology; XV Annual Meeting Argentinean Society for General MicrobiologyArgentinaSociedad Argentina de Investigación Bioquímica y Biología MolecularTech Science Press2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/173528Implication of Sphingosine-1-Phosphate Receptor 2 (S1PR2) in Differentiation and Dedifferentiation of Epithelial Renal Cells; LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology; XV Annual Meeting Argentinean Society for General Microbiology; Argentina; 2020; 1-10327-9545CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.samige.org.ar/admin/news/files/170-TSP_BIOCELL_42376.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:23:03Zoai:ri.conicet.gov.ar:11336/173528instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:23:03.677CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Implication of Sphingosine-1-Phosphate Receptor 2 (S1PR2) in Differentiation and Dedifferentiation of Epithelial Renal Cells |
title |
Implication of Sphingosine-1-Phosphate Receptor 2 (S1PR2) in Differentiation and Dedifferentiation of Epithelial Renal Cells |
spellingShingle |
Implication of Sphingosine-1-Phosphate Receptor 2 (S1PR2) in Differentiation and Dedifferentiation of Epithelial Renal Cells Romero, Daniela Judith EPITHELIAL-MESENCHYMAL TRANSITION SPHINGOSINE-1-PHOSPHATE RECEPTOR 2 SPHINGOSINE 1-PHOSPHATE EPITHELIAL RENAL CELLS |
title_short |
Implication of Sphingosine-1-Phosphate Receptor 2 (S1PR2) in Differentiation and Dedifferentiation of Epithelial Renal Cells |
title_full |
Implication of Sphingosine-1-Phosphate Receptor 2 (S1PR2) in Differentiation and Dedifferentiation of Epithelial Renal Cells |
title_fullStr |
Implication of Sphingosine-1-Phosphate Receptor 2 (S1PR2) in Differentiation and Dedifferentiation of Epithelial Renal Cells |
title_full_unstemmed |
Implication of Sphingosine-1-Phosphate Receptor 2 (S1PR2) in Differentiation and Dedifferentiation of Epithelial Renal Cells |
title_sort |
Implication of Sphingosine-1-Phosphate Receptor 2 (S1PR2) in Differentiation and Dedifferentiation of Epithelial Renal Cells |
dc.creator.none.fl_str_mv |
Romero, Daniela Judith Santacreu, Bruno Jaime Tarallo, Estefania Favale, Nicolas Octavio |
author |
Romero, Daniela Judith |
author_facet |
Romero, Daniela Judith Santacreu, Bruno Jaime Tarallo, Estefania Favale, Nicolas Octavio |
author_role |
author |
author2 |
Santacreu, Bruno Jaime Tarallo, Estefania Favale, Nicolas Octavio |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
EPITHELIAL-MESENCHYMAL TRANSITION SPHINGOSINE-1-PHOSPHATE RECEPTOR 2 SPHINGOSINE 1-PHOSPHATE EPITHELIAL RENAL CELLS |
topic |
EPITHELIAL-MESENCHYMAL TRANSITION SPHINGOSINE-1-PHOSPHATE RECEPTOR 2 SPHINGOSINE 1-PHOSPHATE EPITHELIAL RENAL CELLS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Epithelial cell differentiation is a process that involves the mesenchymal-epithelial transition (MET) and includes cell cycle arrest, cell-cell junction maturation in addition to changes in cell migration capacity. The epithelial-mesenchymal transition (EMT) is a dynamic process by which fully differentiated epithelial cells can acquire a mesenchymal phenotype. During EMT, cell adhesion and apical-basal polarity are lost, and the cytoskeleton is reorganized. Previous results from our laboratory showed that in Madin-Darby canine kidney cells (MDCK) under different culture conditions can achieve different stages of differentiation resembling MET. Sphingosine 1-phosphate (S1P) is a bioactive sphingolipid, produced by the phosphorylation of sphingosine by sphingosine kinases (SKs), which is involved in different processes such as proliferation, cell growth, differentiation, and migration. S1P can act both intracellularly as a second messenger or extracellularly as a ligand of 5 different G protein-coupled receptors (S1PR1-5). In the present work, we evaluated the importance of S1P acting on S1PR2 in the modulation of MET and EMT. We found that there are differences in the action of S1PR2 in MDCK cells that depends on the differentiation stage. S1PR2 positively modulates the passage from polarized to differentiated cells through MET. Inhibition of S1PR2 blocks adherens junction establishment, as well as apical and basal polarity. On the other hand, once cells have acquired the differentiated phenotype, S1PR2 induces the dedifferentiation of epithelial cells through EMT. Inhibition of S1PR2 triggers changes in EMT markers, such as rearrangements of the actin cytoskeleton, expression of vimentin, and nuclear translocation of beta-catenin, as well as Slug. The expression levels of S1PR2 in the different stages of differentiation of MDCK cells did not show significant differences. Instead, immunofluorescence studies showed that during cell differentiation, S1PR2 was progressively enriched at the plasma membrane. These results suggest that the location of S1PR2 depends on the stage of cell differentiation, and this determines its role. These findings highlight the great versatility of S1P on the control of physiological and pathophysiological processes. Fil: Romero, Daniela Judith. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina Fil: Santacreu, Bruno Jaime. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina Fil: Tarallo, Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina Fil: Favale, Nicolas Octavio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology; XV Annual Meeting Argentinean Society for General Microbiology Argentina Sociedad Argentina de Investigación Bioquímica y Biología Molecular |
description |
Epithelial cell differentiation is a process that involves the mesenchymal-epithelial transition (MET) and includes cell cycle arrest, cell-cell junction maturation in addition to changes in cell migration capacity. The epithelial-mesenchymal transition (EMT) is a dynamic process by which fully differentiated epithelial cells can acquire a mesenchymal phenotype. During EMT, cell adhesion and apical-basal polarity are lost, and the cytoskeleton is reorganized. Previous results from our laboratory showed that in Madin-Darby canine kidney cells (MDCK) under different culture conditions can achieve different stages of differentiation resembling MET. Sphingosine 1-phosphate (S1P) is a bioactive sphingolipid, produced by the phosphorylation of sphingosine by sphingosine kinases (SKs), which is involved in different processes such as proliferation, cell growth, differentiation, and migration. S1P can act both intracellularly as a second messenger or extracellularly as a ligand of 5 different G protein-coupled receptors (S1PR1-5). In the present work, we evaluated the importance of S1P acting on S1PR2 in the modulation of MET and EMT. We found that there are differences in the action of S1PR2 in MDCK cells that depends on the differentiation stage. S1PR2 positively modulates the passage from polarized to differentiated cells through MET. Inhibition of S1PR2 blocks adherens junction establishment, as well as apical and basal polarity. On the other hand, once cells have acquired the differentiated phenotype, S1PR2 induces the dedifferentiation of epithelial cells through EMT. Inhibition of S1PR2 triggers changes in EMT markers, such as rearrangements of the actin cytoskeleton, expression of vimentin, and nuclear translocation of beta-catenin, as well as Slug. The expression levels of S1PR2 in the different stages of differentiation of MDCK cells did not show significant differences. Instead, immunofluorescence studies showed that during cell differentiation, S1PR2 was progressively enriched at the plasma membrane. These results suggest that the location of S1PR2 depends on the stage of cell differentiation, and this determines its role. These findings highlight the great versatility of S1P on the control of physiological and pathophysiological processes. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021 |
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http://hdl.handle.net/11336/173528 Implication of Sphingosine-1-Phosphate Receptor 2 (S1PR2) in Differentiation and Dedifferentiation of Epithelial Renal Cells; LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology; XV Annual Meeting Argentinean Society for General Microbiology; Argentina; 2020; 1-1 0327-9545 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/173528 |
identifier_str_mv |
Implication of Sphingosine-1-Phosphate Receptor 2 (S1PR2) in Differentiation and Dedifferentiation of Epithelial Renal Cells; LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology; XV Annual Meeting Argentinean Society for General Microbiology; Argentina; 2020; 1-1 0327-9545 CONICET Digital CONICET |
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eng |
language |
eng |
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Tech Science Press |
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Tech Science Press |
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