Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5

Autores
Maidana, Silvina Soledad; Craig, Patricio Oliver; Craig, María Isabel; Ludwig, Louisa; Mauroy, Axel; Thiry, Etienne; Romera, Sonia Alejandra
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Closely related bovine alphaherpesviruses 1 (BoHV-1) and 5 (BoHV-5) co-circulate in certain countries, rendering cattle co-infection possible. This is a prerequisite for BoHV recombination. Here, we report the first identification of homologous recombination between field isolates of BoHV-1 and BoHV-5, two alphaherpesviruses belonging to two distinct species with an average genomic similarity of 82.3%. Three isolates of BoHV-5, previously classified as subtype “BoHV-5b”, were phylogenetically studied and analyzed via eight PCR sequencing assays dispersed at regular intervals throughout the genome to discriminate between BoHV-1 and BoHV-5. In the phylogenetic analysis, differences of clustering were found in the UL27 gene which encodes the glycoprotein B (gB). We detected two recombination breakpoints in the open reading frame of the UL27 gene. We compared the amino acid sequences of the gB of BoHV-1.1 and 1.2, BoHV-5a and recombinant formerly named BoHV-5b (chimeric gB) and subsequently performed molecular modeling. All structures were alike and, simultaneously, similar to the chimeric gB. Neutralizing antibodies against BoHV-1, BoHV-5 and recombinant viruses were analyzed via serum virus neutralization test using polyclonal sera and a monoclonal antibody against gB to demonstrate an absence of viral escape for both assays. Our results show that homologous recombination between two related species of ruminant alphaherpesviruses can occur in natural field conditions. We found three recombinant field isolates, previously classified as BoHV-5b subtypes, between BoHV-1 and BoHV-5.
Fil: Maidana SS. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Craig, Patricio Oliver. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Ludwig L. University Of Liège; Bélgica
Fil: Mauroy A. University Of Liège; Bélgica
Fil: Thiry E. University Of Liège; Bélgica
Fil: Romera SA. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Materia
ALPHAHERPESVIRUSES
BOVINE HERPESVIRUS 1 (BOHV-1)
BOVINE HERPESVIRUS 5 (BOHV-5)
GLYCOPROTEIN B
NATURAL RECOMBINATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/97925
Acceder
id CONICETDig_aaa77b1fdad81a875fc3d7c6acfbbb9d
oai_identifier_str oai:ri.conicet.gov.ar:11336/97925
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5Maidana, Silvina SoledadCraig, Patricio OliverCraig, María IsabelLudwig, LouisaMauroy, AxelThiry, EtienneRomera, Sonia AlejandraALPHAHERPESVIRUSESBOVINE HERPESVIRUS 1 (BOHV-1)BOVINE HERPESVIRUS 5 (BOHV-5)GLYCOPROTEIN BNATURAL RECOMBINATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Closely related bovine alphaherpesviruses 1 (BoHV-1) and 5 (BoHV-5) co-circulate in certain countries, rendering cattle co-infection possible. This is a prerequisite for BoHV recombination. Here, we report the first identification of homologous recombination between field isolates of BoHV-1 and BoHV-5, two alphaherpesviruses belonging to two distinct species with an average genomic similarity of 82.3%. Three isolates of BoHV-5, previously classified as subtype “BoHV-5b”, were phylogenetically studied and analyzed via eight PCR sequencing assays dispersed at regular intervals throughout the genome to discriminate between BoHV-1 and BoHV-5. In the phylogenetic analysis, differences of clustering were found in the UL27 gene which encodes the glycoprotein B (gB). We detected two recombination breakpoints in the open reading frame of the UL27 gene. We compared the amino acid sequences of the gB of BoHV-1.1 and 1.2, BoHV-5a and recombinant formerly named BoHV-5b (chimeric gB) and subsequently performed molecular modeling. All structures were alike and, simultaneously, similar to the chimeric gB. Neutralizing antibodies against BoHV-1, BoHV-5 and recombinant viruses were analyzed via serum virus neutralization test using polyclonal sera and a monoclonal antibody against gB to demonstrate an absence of viral escape for both assays. Our results show that homologous recombination between two related species of ruminant alphaherpesviruses can occur in natural field conditions. We found three recombinant field isolates, previously classified as BoHV-5b subtypes, between BoHV-1 and BoHV-5.Fil: Maidana SS. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; ArgentinaFil: Craig, Patricio Oliver. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; ArgentinaFil: Ludwig L. University Of Liège; BélgicaFil: Mauroy A. University Of Liège; BélgicaFil: Thiry E. University Of Liège; BélgicaFil: Romera SA. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; ArgentinaElsevier Science2017-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/97925Maidana, Silvina Soledad; Craig, Patricio Oliver; Craig, María Isabel; Ludwig, Louisa; Mauroy, Axel; et al.; Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5; Elsevier Science; Virus Research; 242; 10-2017; 122-1300168-1702CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0168170217302976info:eu-repo/semantics/altIdentifier/doi/10.1016/j.virusres.2017.09.018info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:52:15Zoai:ri.conicet.gov.ar:11336/97925instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:52:15.667CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5
title Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5
spellingShingle Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5
Maidana, Silvina Soledad
ALPHAHERPESVIRUSES
BOVINE HERPESVIRUS 1 (BOHV-1)
BOVINE HERPESVIRUS 5 (BOHV-5)
GLYCOPROTEIN B
NATURAL RECOMBINATION
title_short Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5
title_full Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5
title_fullStr Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5
title_full_unstemmed Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5
title_sort Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5
dc.creator.none.fl_str_mv Maidana, Silvina Soledad
Craig, Patricio Oliver
Craig, María Isabel
Ludwig, Louisa
Mauroy, Axel
Thiry, Etienne
Romera, Sonia Alejandra
author Maidana, Silvina Soledad
author_facet Maidana, Silvina Soledad
Craig, Patricio Oliver
Craig, María Isabel
Ludwig, Louisa
Mauroy, Axel
Thiry, Etienne
Romera, Sonia Alejandra
author_role author
author2 Craig, Patricio Oliver
Craig, María Isabel
Ludwig, Louisa
Mauroy, Axel
Thiry, Etienne
Romera, Sonia Alejandra
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ALPHAHERPESVIRUSES
BOVINE HERPESVIRUS 1 (BOHV-1)
BOVINE HERPESVIRUS 5 (BOHV-5)
GLYCOPROTEIN B
NATURAL RECOMBINATION
topic ALPHAHERPESVIRUSES
BOVINE HERPESVIRUS 1 (BOHV-1)
BOVINE HERPESVIRUS 5 (BOHV-5)
GLYCOPROTEIN B
NATURAL RECOMBINATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Closely related bovine alphaherpesviruses 1 (BoHV-1) and 5 (BoHV-5) co-circulate in certain countries, rendering cattle co-infection possible. This is a prerequisite for BoHV recombination. Here, we report the first identification of homologous recombination between field isolates of BoHV-1 and BoHV-5, two alphaherpesviruses belonging to two distinct species with an average genomic similarity of 82.3%. Three isolates of BoHV-5, previously classified as subtype “BoHV-5b”, were phylogenetically studied and analyzed via eight PCR sequencing assays dispersed at regular intervals throughout the genome to discriminate between BoHV-1 and BoHV-5. In the phylogenetic analysis, differences of clustering were found in the UL27 gene which encodes the glycoprotein B (gB). We detected two recombination breakpoints in the open reading frame of the UL27 gene. We compared the amino acid sequences of the gB of BoHV-1.1 and 1.2, BoHV-5a and recombinant formerly named BoHV-5b (chimeric gB) and subsequently performed molecular modeling. All structures were alike and, simultaneously, similar to the chimeric gB. Neutralizing antibodies against BoHV-1, BoHV-5 and recombinant viruses were analyzed via serum virus neutralization test using polyclonal sera and a monoclonal antibody against gB to demonstrate an absence of viral escape for both assays. Our results show that homologous recombination between two related species of ruminant alphaherpesviruses can occur in natural field conditions. We found three recombinant field isolates, previously classified as BoHV-5b subtypes, between BoHV-1 and BoHV-5.
Fil: Maidana SS. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Craig, Patricio Oliver. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Ludwig L. University Of Liège; Bélgica
Fil: Mauroy A. University Of Liège; Bélgica
Fil: Thiry E. University Of Liège; Bélgica
Fil: Romera SA. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
description Closely related bovine alphaherpesviruses 1 (BoHV-1) and 5 (BoHV-5) co-circulate in certain countries, rendering cattle co-infection possible. This is a prerequisite for BoHV recombination. Here, we report the first identification of homologous recombination between field isolates of BoHV-1 and BoHV-5, two alphaherpesviruses belonging to two distinct species with an average genomic similarity of 82.3%. Three isolates of BoHV-5, previously classified as subtype “BoHV-5b”, were phylogenetically studied and analyzed via eight PCR sequencing assays dispersed at regular intervals throughout the genome to discriminate between BoHV-1 and BoHV-5. In the phylogenetic analysis, differences of clustering were found in the UL27 gene which encodes the glycoprotein B (gB). We detected two recombination breakpoints in the open reading frame of the UL27 gene. We compared the amino acid sequences of the gB of BoHV-1.1 and 1.2, BoHV-5a and recombinant formerly named BoHV-5b (chimeric gB) and subsequently performed molecular modeling. All structures were alike and, simultaneously, similar to the chimeric gB. Neutralizing antibodies against BoHV-1, BoHV-5 and recombinant viruses were analyzed via serum virus neutralization test using polyclonal sera and a monoclonal antibody against gB to demonstrate an absence of viral escape for both assays. Our results show that homologous recombination between two related species of ruminant alphaherpesviruses can occur in natural field conditions. We found three recombinant field isolates, previously classified as BoHV-5b subtypes, between BoHV-1 and BoHV-5.
publishDate 2017
dc.date.none.fl_str_mv 2017-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/97925
Maidana, Silvina Soledad; Craig, Patricio Oliver; Craig, María Isabel; Ludwig, Louisa; Mauroy, Axel; et al.; Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5; Elsevier Science; Virus Research; 242; 10-2017; 122-130
0168-1702
CONICET Digital
CONICET
url http://hdl.handle.net/11336/97925
identifier_str_mv Maidana, Silvina Soledad; Craig, Patricio Oliver; Craig, María Isabel; Ludwig, Louisa; Mauroy, Axel; et al.; Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5; Elsevier Science; Virus Research; 242; 10-2017; 122-130
0168-1702
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0168170217302976
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.virusres.2017.09.018
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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