Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5

Autores
Maidana, Silvina Soledad; Craig, Patricio Oliver; Craig, María Isabel; Ludwig, Louisa; Mauroy, Axel; Thiry, Etienne; Romera, Sonia Alejandra
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Closely related bovine alphaherpesviruses 1 (BoHV-1) and 5 (BoHV-5) co-circulate in certain countries, rendering cattle co-infection possible. This is a prerequisite for BoHV recombination. Here, we report the first identification of homologous recombination between field isolates of BoHV-1 and BoHV-5, two alphaherpesviruses belonging to two distinct species with an average genomic similarity of 82.3%. Three isolates of BoHV-5, previously classified as subtype “BoHV-5b”, were phylogenetically studied and analyzed via eight PCR sequencing assays dispersed at regular intervals throughout the genome to discriminate between BoHV-1 andBoHV-5. In the phylogenetic analysis, differences of clustering were found in the UL27 gene which encodes the glycoprotein B (gB). We detected two recombination breakpoints in the open reading frame of the UL27 gene. We compared the amino acid sequences of the gB of BoHV-1.1 and 1.2, BoHV-5a and recombinant formerly named BoHV-5b (chimeric gB) and subsequently performed molecular modeling. All structures were alike and, simultaneously, similar to the chimeric gB. Neutralizing antibodies against BoHV-1, BoHV-5 and recombinant viruses were analyzed via serum virus neutralization test using polyclonal sera and a monoclonal antibody against gB to demonstrate an absence of viral escape for both assays. Our results show that homologous recombination between two related species of ruminant alphaherpesviruses can occur in natural field conditions. We found three recombinant field isolates, previously classified as BoHV-5b subtypes, between BoHV-1 and BoHV-5.
Inst.de Virología
Fil: Maidana, Silvina Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales. Cátedra de Inmunogenética; Argentina
Fil: Craig, Patricio Oliver. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Biológica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil: Craig, María Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Ludwig, Louisa. University of Liège. Fundamental and Applied Research on Animal Health Center and Faculty of Veterinary Medicine. Veterinary Virology and Animal Viral Diseases; Bélgica
Fil: Mauroy, Axel. University of Liège. Fundamental and Applied Research on Animal Health Center and Faculty of Veterinary Medicine. Veterinary Virology and Animal Viral Diseases; Bélgica
Fil: Thiry, Etienne. University of Liège. Fundamental and Applied Research on Animal Health Center and Faculty of Veterinary Medicine. Veterinary Virology and Animal Viral Diseases; Bélgica
Fil: Romera, Sonia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales. Cátedra de Inmunogenética; Argentina. Universidad del Salvador. Cátedra de Inmunología; Argentina
Fuente
Virus research 242 : 122-130. (October 2017)
Materia
Ganado Bovino
Enfermedades de los Animales
Herpesviridae
Herpes Virus Bovino
Cattle
Animal Diseases
Bovine Herpesvirus
Herpesvirus
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/2000
Acceder
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oai_identifier_str oai:localhost:20.500.12123/2000
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5Maidana, Silvina SoledadCraig, Patricio OliverCraig, María IsabelLudwig, LouisaMauroy, AxelThiry, EtienneRomera, Sonia AlejandraGanado BovinoEnfermedades de los AnimalesHerpesviridaeHerpes Virus BovinoCattleAnimal DiseasesBovine HerpesvirusHerpesvirusClosely related bovine alphaherpesviruses 1 (BoHV-1) and 5 (BoHV-5) co-circulate in certain countries, rendering cattle co-infection possible. This is a prerequisite for BoHV recombination. Here, we report the first identification of homologous recombination between field isolates of BoHV-1 and BoHV-5, two alphaherpesviruses belonging to two distinct species with an average genomic similarity of 82.3%. Three isolates of BoHV-5, previously classified as subtype “BoHV-5b”, were phylogenetically studied and analyzed via eight PCR sequencing assays dispersed at regular intervals throughout the genome to discriminate between BoHV-1 andBoHV-5. In the phylogenetic analysis, differences of clustering were found in the UL27 gene which encodes the glycoprotein B (gB). We detected two recombination breakpoints in the open reading frame of the UL27 gene. We compared the amino acid sequences of the gB of BoHV-1.1 and 1.2, BoHV-5a and recombinant formerly named BoHV-5b (chimeric gB) and subsequently performed molecular modeling. All structures were alike and, simultaneously, similar to the chimeric gB. Neutralizing antibodies against BoHV-1, BoHV-5 and recombinant viruses were analyzed via serum virus neutralization test using polyclonal sera and a monoclonal antibody against gB to demonstrate an absence of viral escape for both assays. Our results show that homologous recombination between two related species of ruminant alphaherpesviruses can occur in natural field conditions. We found three recombinant field isolates, previously classified as BoHV-5b subtypes, between BoHV-1 and BoHV-5.Inst.de VirologíaFil: Maidana, Silvina Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales. Cátedra de Inmunogenética; ArgentinaFil: Craig, Patricio Oliver. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Biológica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil: Craig, María Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Ludwig, Louisa. University of Liège. Fundamental and Applied Research on Animal Health Center and Faculty of Veterinary Medicine. Veterinary Virology and Animal Viral Diseases; BélgicaFil: Mauroy, Axel. University of Liège. Fundamental and Applied Research on Animal Health Center and Faculty of Veterinary Medicine. Veterinary Virology and Animal Viral Diseases; BélgicaFil: Thiry, Etienne. University of Liège. Fundamental and Applied Research on Animal Health Center and Faculty of Veterinary Medicine. Veterinary Virology and Animal Viral Diseases; BélgicaFil: Romera, Sonia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales. Cátedra de Inmunogenética; Argentina. Universidad del Salvador. Cátedra de Inmunología; Argentina2018-03-09T11:25:58Z2018-03-09T11:25:58Z2017-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/2000https://www.sciencedirect.com/science/article/pii/S0168170217302976?via%3Dihub0168-1702https://doi.org/10.1016/j.virusres.2017.09.018Virus research 242 : 122-130. (October 2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:15Zoai:localhost:20.500.12123/2000instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:15.875INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5
title Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5
spellingShingle Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5
Maidana, Silvina Soledad
Ganado Bovino
Enfermedades de los Animales
Herpesviridae
Herpes Virus Bovino
Cattle
Animal Diseases
Bovine Herpesvirus
Herpesvirus
title_short Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5
title_full Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5
title_fullStr Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5
title_full_unstemmed Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5
title_sort Evidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5
dc.creator.none.fl_str_mv Maidana, Silvina Soledad
Craig, Patricio Oliver
Craig, María Isabel
Ludwig, Louisa
Mauroy, Axel
Thiry, Etienne
Romera, Sonia Alejandra
author Maidana, Silvina Soledad
author_facet Maidana, Silvina Soledad
Craig, Patricio Oliver
Craig, María Isabel
Ludwig, Louisa
Mauroy, Axel
Thiry, Etienne
Romera, Sonia Alejandra
author_role author
author2 Craig, Patricio Oliver
Craig, María Isabel
Ludwig, Louisa
Mauroy, Axel
Thiry, Etienne
Romera, Sonia Alejandra
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Ganado Bovino
Enfermedades de los Animales
Herpesviridae
Herpes Virus Bovino
Cattle
Animal Diseases
Bovine Herpesvirus
Herpesvirus
topic Ganado Bovino
Enfermedades de los Animales
Herpesviridae
Herpes Virus Bovino
Cattle
Animal Diseases
Bovine Herpesvirus
Herpesvirus
dc.description.none.fl_txt_mv Closely related bovine alphaherpesviruses 1 (BoHV-1) and 5 (BoHV-5) co-circulate in certain countries, rendering cattle co-infection possible. This is a prerequisite for BoHV recombination. Here, we report the first identification of homologous recombination between field isolates of BoHV-1 and BoHV-5, two alphaherpesviruses belonging to two distinct species with an average genomic similarity of 82.3%. Three isolates of BoHV-5, previously classified as subtype “BoHV-5b”, were phylogenetically studied and analyzed via eight PCR sequencing assays dispersed at regular intervals throughout the genome to discriminate between BoHV-1 andBoHV-5. In the phylogenetic analysis, differences of clustering were found in the UL27 gene which encodes the glycoprotein B (gB). We detected two recombination breakpoints in the open reading frame of the UL27 gene. We compared the amino acid sequences of the gB of BoHV-1.1 and 1.2, BoHV-5a and recombinant formerly named BoHV-5b (chimeric gB) and subsequently performed molecular modeling. All structures were alike and, simultaneously, similar to the chimeric gB. Neutralizing antibodies against BoHV-1, BoHV-5 and recombinant viruses were analyzed via serum virus neutralization test using polyclonal sera and a monoclonal antibody against gB to demonstrate an absence of viral escape for both assays. Our results show that homologous recombination between two related species of ruminant alphaherpesviruses can occur in natural field conditions. We found three recombinant field isolates, previously classified as BoHV-5b subtypes, between BoHV-1 and BoHV-5.
Inst.de Virología
Fil: Maidana, Silvina Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales. Cátedra de Inmunogenética; Argentina
Fil: Craig, Patricio Oliver. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Biológica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil: Craig, María Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Ludwig, Louisa. University of Liège. Fundamental and Applied Research on Animal Health Center and Faculty of Veterinary Medicine. Veterinary Virology and Animal Viral Diseases; Bélgica
Fil: Mauroy, Axel. University of Liège. Fundamental and Applied Research on Animal Health Center and Faculty of Veterinary Medicine. Veterinary Virology and Animal Viral Diseases; Bélgica
Fil: Thiry, Etienne. University of Liège. Fundamental and Applied Research on Animal Health Center and Faculty of Veterinary Medicine. Veterinary Virology and Animal Viral Diseases; Bélgica
Fil: Romera, Sonia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales. Cátedra de Inmunogenética; Argentina. Universidad del Salvador. Cátedra de Inmunología; Argentina
description Closely related bovine alphaherpesviruses 1 (BoHV-1) and 5 (BoHV-5) co-circulate in certain countries, rendering cattle co-infection possible. This is a prerequisite for BoHV recombination. Here, we report the first identification of homologous recombination between field isolates of BoHV-1 and BoHV-5, two alphaherpesviruses belonging to two distinct species with an average genomic similarity of 82.3%. Three isolates of BoHV-5, previously classified as subtype “BoHV-5b”, were phylogenetically studied and analyzed via eight PCR sequencing assays dispersed at regular intervals throughout the genome to discriminate between BoHV-1 andBoHV-5. In the phylogenetic analysis, differences of clustering were found in the UL27 gene which encodes the glycoprotein B (gB). We detected two recombination breakpoints in the open reading frame of the UL27 gene. We compared the amino acid sequences of the gB of BoHV-1.1 and 1.2, BoHV-5a and recombinant formerly named BoHV-5b (chimeric gB) and subsequently performed molecular modeling. All structures were alike and, simultaneously, similar to the chimeric gB. Neutralizing antibodies against BoHV-1, BoHV-5 and recombinant viruses were analyzed via serum virus neutralization test using polyclonal sera and a monoclonal antibody against gB to demonstrate an absence of viral escape for both assays. Our results show that homologous recombination between two related species of ruminant alphaherpesviruses can occur in natural field conditions. We found three recombinant field isolates, previously classified as BoHV-5b subtypes, between BoHV-1 and BoHV-5.
publishDate 2017
dc.date.none.fl_str_mv 2017-10
2018-03-09T11:25:58Z
2018-03-09T11:25:58Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/2000
https://www.sciencedirect.com/science/article/pii/S0168170217302976?via%3Dihub
0168-1702
https://doi.org/10.1016/j.virusres.2017.09.018
url http://hdl.handle.net/20.500.12123/2000
https://www.sciencedirect.com/science/article/pii/S0168170217302976?via%3Dihub
https://doi.org/10.1016/j.virusres.2017.09.018
identifier_str_mv 0168-1702
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Virus research 242 : 122-130. (October 2017)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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