Humoral immune response of mice against a vaccine candidate composed of a chimera of gB of bovine alphaherpesviruses 1 and 5

Autores
Quintero Barbosa, Juan Sebastián; Alméciga Díaz, Carlos Javier; Perez, Sandra; Gutierrez, María Fernanda
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Infectious bovine rhinotracheitis (IBR) and bovine meningoencephalitis are caused by Bovine alphaherpesvirus (BoHV) types 1 and 5, which seriously threaten the global cattle industry. Vaccination to improve immunity is the most direct and effective means to prevent these conditions. Glycoprotein B (gB) is essential for the attachment of both viruses to permissive cells, and is a major target of the host immune system, inducing a strong humoral response. The aim of this study was to evaluate, in a murine model, the immune response of a candidate vaccine formulation composed of a chimeric BoHV-1 and BoHV-5 gB (DgB), expressed in Komagataella phaffii. The chimeric DgB vaccine adjuvanted with Montanide 50 ISA V2 or aluminum hydroxide was administered intramuscularly or subcutaneously. A control group and a group that received a commercial vaccine were inoculated subcutaneously. Higher titers of neutralizing antibodies against BoHV-1, BoHV-5, and a natural BoHV-1/5 recombinant strain were obtained with the oil-based candidate vaccine formulation administered intramuscularly. The results demonstrated that the chimeric DgB conserved important epitopes that were able to stimulate a humoral immune response capable of neutralizing BoHV-1, BoHV-5, and the recombinant strain, suggesting that the vaccine antigen is a promising candidate to be further evaluated in cattle.
Fil: Quintero Barbosa, Juan Sebastián. Pontificia Universidad Javeriana; Colombia
Fil: Alméciga Díaz, Carlos Javier. Pontificia Universidad Javeriana; Colombia
Fil: Perez, Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Gutierrez, María Fernanda. Pontificia Universidad Javeriana; Colombia
Materia
ANTIBODIES
BOVINE ALPHAHERPESVIRUS
BOVINE ALPHAHERPESVIRUS 1 AND 5
CANDIDATE VACCINE
GLYCOPROTEIN B
NEUTRALIZING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/218342
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/218342
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Humoral immune response of mice against a vaccine candidate composed of a chimera of gB of bovine alphaherpesviruses 1 and 5Quintero Barbosa, Juan SebastiánAlméciga Díaz, Carlos JavierPerez, SandraGutierrez, María FernandaANTIBODIESBOVINE ALPHAHERPESVIRUSBOVINE ALPHAHERPESVIRUS 1 AND 5CANDIDATE VACCINEGLYCOPROTEIN BNEUTRALIZINGhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Infectious bovine rhinotracheitis (IBR) and bovine meningoencephalitis are caused by Bovine alphaherpesvirus (BoHV) types 1 and 5, which seriously threaten the global cattle industry. Vaccination to improve immunity is the most direct and effective means to prevent these conditions. Glycoprotein B (gB) is essential for the attachment of both viruses to permissive cells, and is a major target of the host immune system, inducing a strong humoral response. The aim of this study was to evaluate, in a murine model, the immune response of a candidate vaccine formulation composed of a chimeric BoHV-1 and BoHV-5 gB (DgB), expressed in Komagataella phaffii. The chimeric DgB vaccine adjuvanted with Montanide 50 ISA V2 or aluminum hydroxide was administered intramuscularly or subcutaneously. A control group and a group that received a commercial vaccine were inoculated subcutaneously. Higher titers of neutralizing antibodies against BoHV-1, BoHV-5, and a natural BoHV-1/5 recombinant strain were obtained with the oil-based candidate vaccine formulation administered intramuscularly. The results demonstrated that the chimeric DgB conserved important epitopes that were able to stimulate a humoral immune response capable of neutralizing BoHV-1, BoHV-5, and the recombinant strain, suggesting that the vaccine antigen is a promising candidate to be further evaluated in cattle.Fil: Quintero Barbosa, Juan Sebastián. Pontificia Universidad Javeriana; ColombiaFil: Alméciga Díaz, Carlos Javier. Pontificia Universidad Javeriana; ColombiaFil: Perez, Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Gutierrez, María Fernanda. Pontificia Universidad Javeriana; ColombiaMultidisciplinary Digital Publishing Institute (MDPI)2023-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/218342Quintero Barbosa, Juan Sebastián; Alméciga Díaz, Carlos Javier; Perez, Sandra; Gutierrez, María Fernanda; Humoral immune response of mice against a vaccine candidate composed of a chimera of gB of bovine alphaherpesviruses 1 and 5; Multidisciplinary Digital Publishing Institute (MDPI); Vaccines; 11; 7; 7-2023; 1-112076-393XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-393X/11/7/1173info:eu-repo/semantics/altIdentifier/doi/10.3390/vaccines11071173info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:12:40Zoai:ri.conicet.gov.ar:11336/218342instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:12:40.594CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Humoral immune response of mice against a vaccine candidate composed of a chimera of gB of bovine alphaherpesviruses 1 and 5
title Humoral immune response of mice against a vaccine candidate composed of a chimera of gB of bovine alphaherpesviruses 1 and 5
spellingShingle Humoral immune response of mice against a vaccine candidate composed of a chimera of gB of bovine alphaherpesviruses 1 and 5
Quintero Barbosa, Juan Sebastián
ANTIBODIES
BOVINE ALPHAHERPESVIRUS
BOVINE ALPHAHERPESVIRUS 1 AND 5
CANDIDATE VACCINE
GLYCOPROTEIN B
NEUTRALIZING
title_short Humoral immune response of mice against a vaccine candidate composed of a chimera of gB of bovine alphaherpesviruses 1 and 5
title_full Humoral immune response of mice against a vaccine candidate composed of a chimera of gB of bovine alphaherpesviruses 1 and 5
title_fullStr Humoral immune response of mice against a vaccine candidate composed of a chimera of gB of bovine alphaherpesviruses 1 and 5
title_full_unstemmed Humoral immune response of mice against a vaccine candidate composed of a chimera of gB of bovine alphaherpesviruses 1 and 5
title_sort Humoral immune response of mice against a vaccine candidate composed of a chimera of gB of bovine alphaherpesviruses 1 and 5
dc.creator.none.fl_str_mv Quintero Barbosa, Juan Sebastián
Alméciga Díaz, Carlos Javier
Perez, Sandra
Gutierrez, María Fernanda
author Quintero Barbosa, Juan Sebastián
author_facet Quintero Barbosa, Juan Sebastián
Alméciga Díaz, Carlos Javier
Perez, Sandra
Gutierrez, María Fernanda
author_role author
author2 Alméciga Díaz, Carlos Javier
Perez, Sandra
Gutierrez, María Fernanda
author2_role author
author
author
dc.subject.none.fl_str_mv ANTIBODIES
BOVINE ALPHAHERPESVIRUS
BOVINE ALPHAHERPESVIRUS 1 AND 5
CANDIDATE VACCINE
GLYCOPROTEIN B
NEUTRALIZING
topic ANTIBODIES
BOVINE ALPHAHERPESVIRUS
BOVINE ALPHAHERPESVIRUS 1 AND 5
CANDIDATE VACCINE
GLYCOPROTEIN B
NEUTRALIZING
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Infectious bovine rhinotracheitis (IBR) and bovine meningoencephalitis are caused by Bovine alphaherpesvirus (BoHV) types 1 and 5, which seriously threaten the global cattle industry. Vaccination to improve immunity is the most direct and effective means to prevent these conditions. Glycoprotein B (gB) is essential for the attachment of both viruses to permissive cells, and is a major target of the host immune system, inducing a strong humoral response. The aim of this study was to evaluate, in a murine model, the immune response of a candidate vaccine formulation composed of a chimeric BoHV-1 and BoHV-5 gB (DgB), expressed in Komagataella phaffii. The chimeric DgB vaccine adjuvanted with Montanide 50 ISA V2 or aluminum hydroxide was administered intramuscularly or subcutaneously. A control group and a group that received a commercial vaccine were inoculated subcutaneously. Higher titers of neutralizing antibodies against BoHV-1, BoHV-5, and a natural BoHV-1/5 recombinant strain were obtained with the oil-based candidate vaccine formulation administered intramuscularly. The results demonstrated that the chimeric DgB conserved important epitopes that were able to stimulate a humoral immune response capable of neutralizing BoHV-1, BoHV-5, and the recombinant strain, suggesting that the vaccine antigen is a promising candidate to be further evaluated in cattle.
Fil: Quintero Barbosa, Juan Sebastián. Pontificia Universidad Javeriana; Colombia
Fil: Alméciga Díaz, Carlos Javier. Pontificia Universidad Javeriana; Colombia
Fil: Perez, Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Gutierrez, María Fernanda. Pontificia Universidad Javeriana; Colombia
description Infectious bovine rhinotracheitis (IBR) and bovine meningoencephalitis are caused by Bovine alphaherpesvirus (BoHV) types 1 and 5, which seriously threaten the global cattle industry. Vaccination to improve immunity is the most direct and effective means to prevent these conditions. Glycoprotein B (gB) is essential for the attachment of both viruses to permissive cells, and is a major target of the host immune system, inducing a strong humoral response. The aim of this study was to evaluate, in a murine model, the immune response of a candidate vaccine formulation composed of a chimeric BoHV-1 and BoHV-5 gB (DgB), expressed in Komagataella phaffii. The chimeric DgB vaccine adjuvanted with Montanide 50 ISA V2 or aluminum hydroxide was administered intramuscularly or subcutaneously. A control group and a group that received a commercial vaccine were inoculated subcutaneously. Higher titers of neutralizing antibodies against BoHV-1, BoHV-5, and a natural BoHV-1/5 recombinant strain were obtained with the oil-based candidate vaccine formulation administered intramuscularly. The results demonstrated that the chimeric DgB conserved important epitopes that were able to stimulate a humoral immune response capable of neutralizing BoHV-1, BoHV-5, and the recombinant strain, suggesting that the vaccine antigen is a promising candidate to be further evaluated in cattle.
publishDate 2023
dc.date.none.fl_str_mv 2023-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/218342
Quintero Barbosa, Juan Sebastián; Alméciga Díaz, Carlos Javier; Perez, Sandra; Gutierrez, María Fernanda; Humoral immune response of mice against a vaccine candidate composed of a chimera of gB of bovine alphaherpesviruses 1 and 5; Multidisciplinary Digital Publishing Institute (MDPI); Vaccines; 11; 7; 7-2023; 1-11
2076-393X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/218342
identifier_str_mv Quintero Barbosa, Juan Sebastián; Alméciga Díaz, Carlos Javier; Perez, Sandra; Gutierrez, María Fernanda; Humoral immune response of mice against a vaccine candidate composed of a chimera of gB of bovine alphaherpesviruses 1 and 5; Multidisciplinary Digital Publishing Institute (MDPI); Vaccines; 11; 7; 7-2023; 1-11
2076-393X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-393X/11/7/1173
info:eu-repo/semantics/altIdentifier/doi/10.3390/vaccines11071173
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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