Inside the gut: Revelations of metatranscriptomic and transcriptomic analyses of Spodoptera frugiperda larvae from an organic maize plantation at 2283 meters above sea level (Tafí...

Autores
Rozadilla, Gastón; Cabrera, Natalia Alina; Virla, Eduardo Gabriel; Mccarthy, Cristina Beryl
Año de publicación
2018
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Spodoptera frugiperda is a noctuid moth that devastates various crops, including corn, and is found in most of the American continent. Transgenic crops that produce insecticidal proteins from Bacillus thuringiensis are currently the most successful biotechnological pest management application, but S. frugiperda has developed field-evolved resistance. In this regard, insect gut microbiota conform a complex community that establishes symbiotic relationships with its host, contributing to its viability. For this reason, metatranscriptomic and transcriptomic analyses of insect guts in their natural environment are invaluable to better comprehend their biology and to identify genes as targets for pest control. We previously captured S. frugiperda specimens from different environments, altitudes and food sources in the province of Tucumán (Argentina). For all samples, total RNA extracted from fifth instar larval guts was submitted to a one-step reverse transcription and PCR sequence independent amplification procedure, and then pyrosequenced. In this study we analysed one of these samples, namely, larvae that were captured in an organic maize field in Tafí del Valle (26°55´40.75´´S, 65°45´19.90´´W; Tucumán province) at 2283 meters above sea level. Sequence reads were trimmed and assembled. Homology searches were performed against various NCBI databases. Taxonomic and functional contents were analysed with MEGAN. The metatranscriptome, in which we identified sequences from archaea, bacteria, fungi, nematodes and plants, revealed potential biocontrol candidates for this pest, and others related with host metabolism and digestion. Furthermore, the host transcriptome showed that most transcripts were associated with the digestive tract structure and development, among others. Some of these genes could be possible targets for pest control via RNA interference (RNAi). In summary, this study has shown the potential effects of this particular food source (i.e., organic maize) and of the environmental conditions (altitude, among others), on the expression profile of S. frugiperda larval guts, their associated metatranscriptome, and putative interactions between them. Future studies will test the potential biocontrol candidates that we identified.
Fil: Rozadilla, Gastón. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cabrera, Natalia Alina. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina
Fil: Virla, Eduardo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Mccarthy, Cristina Beryl. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina
X Congreso Argentino de Entomología
Mendoza
Argentina
Sociedad Argentina de Entomología
Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias
Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas
Instituto Nacional de Tecnología Agropecuaria
Materia
METATRASCRIPTOMICS
SPODOPTERA
MAIZE
MICROBIOTA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/187930
Acceder
id CONICETDig_aa1519b07e809ba24ffc19d0240e5fec
oai_identifier_str oai:ri.conicet.gov.ar:11336/187930
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Inside the gut: Revelations of metatranscriptomic and transcriptomic analyses of Spodoptera frugiperda larvae from an organic maize plantation at 2283 meters above sea level (Tafí del Valle, Tucumán)Rozadilla, GastónCabrera, Natalia AlinaVirla, Eduardo GabrielMccarthy, Cristina BerylMETATRASCRIPTOMICSSPODOPTERAMAIZEMICROBIOTAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Spodoptera frugiperda is a noctuid moth that devastates various crops, including corn, and is found in most of the American continent. Transgenic crops that produce insecticidal proteins from Bacillus thuringiensis are currently the most successful biotechnological pest management application, but S. frugiperda has developed field-evolved resistance. In this regard, insect gut microbiota conform a complex community that establishes symbiotic relationships with its host, contributing to its viability. For this reason, metatranscriptomic and transcriptomic analyses of insect guts in their natural environment are invaluable to better comprehend their biology and to identify genes as targets for pest control. We previously captured S. frugiperda specimens from different environments, altitudes and food sources in the province of Tucumán (Argentina). For all samples, total RNA extracted from fifth instar larval guts was submitted to a one-step reverse transcription and PCR sequence independent amplification procedure, and then pyrosequenced. In this study we analysed one of these samples, namely, larvae that were captured in an organic maize field in Tafí del Valle (26°55´40.75´´S, 65°45´19.90´´W; Tucumán province) at 2283 meters above sea level. Sequence reads were trimmed and assembled. Homology searches were performed against various NCBI databases. Taxonomic and functional contents were analysed with MEGAN. The metatranscriptome, in which we identified sequences from archaea, bacteria, fungi, nematodes and plants, revealed potential biocontrol candidates for this pest, and others related with host metabolism and digestion. Furthermore, the host transcriptome showed that most transcripts were associated with the digestive tract structure and development, among others. Some of these genes could be possible targets for pest control via RNA interference (RNAi). In summary, this study has shown the potential effects of this particular food source (i.e., organic maize) and of the environmental conditions (altitude, among others), on the expression profile of S. frugiperda larval guts, their associated metatranscriptome, and putative interactions between them. Future studies will test the potential biocontrol candidates that we identified.Fil: Rozadilla, Gastón. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cabrera, Natalia Alina. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; ArgentinaFil: Virla, Eduardo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Mccarthy, Cristina Beryl. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; ArgentinaX Congreso Argentino de EntomologíaMendozaArgentinaSociedad Argentina de EntomologíaUniversidad Nacional de Cuyo. Facultad de Ciencias AgrariasUniversidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas ÁridasInstituto Nacional de Tecnología AgropecuariaUniversidad Nacional de Cuyo2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/187930Inside the gut: Revelations of metatranscriptomic and transcriptomic analyses of Spodoptera frugiperda larvae from an organic maize plantation at 2283 meters above sea level (Tafí del Valle, Tucumán); X Congreso Argentino de Entomología; Mendoza; Argentina; 2018; 1-2978-987-575-179-8CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://xcaeorg.files.wordpress.com/2018/06/libro-de-resumenes-xcae-2018.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:47:15Zoai:ri.conicet.gov.ar:11336/187930instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:47:15.682CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Inside the gut: Revelations of metatranscriptomic and transcriptomic analyses of Spodoptera frugiperda larvae from an organic maize plantation at 2283 meters above sea level (Tafí del Valle, Tucumán)
title Inside the gut: Revelations of metatranscriptomic and transcriptomic analyses of Spodoptera frugiperda larvae from an organic maize plantation at 2283 meters above sea level (Tafí del Valle, Tucumán)
spellingShingle Inside the gut: Revelations of metatranscriptomic and transcriptomic analyses of Spodoptera frugiperda larvae from an organic maize plantation at 2283 meters above sea level (Tafí del Valle, Tucumán)
Rozadilla, Gastón
METATRASCRIPTOMICS
SPODOPTERA
MAIZE
MICROBIOTA
title_short Inside the gut: Revelations of metatranscriptomic and transcriptomic analyses of Spodoptera frugiperda larvae from an organic maize plantation at 2283 meters above sea level (Tafí del Valle, Tucumán)
title_full Inside the gut: Revelations of metatranscriptomic and transcriptomic analyses of Spodoptera frugiperda larvae from an organic maize plantation at 2283 meters above sea level (Tafí del Valle, Tucumán)
title_fullStr Inside the gut: Revelations of metatranscriptomic and transcriptomic analyses of Spodoptera frugiperda larvae from an organic maize plantation at 2283 meters above sea level (Tafí del Valle, Tucumán)
title_full_unstemmed Inside the gut: Revelations of metatranscriptomic and transcriptomic analyses of Spodoptera frugiperda larvae from an organic maize plantation at 2283 meters above sea level (Tafí del Valle, Tucumán)
title_sort Inside the gut: Revelations of metatranscriptomic and transcriptomic analyses of Spodoptera frugiperda larvae from an organic maize plantation at 2283 meters above sea level (Tafí del Valle, Tucumán)
dc.creator.none.fl_str_mv Rozadilla, Gastón
Cabrera, Natalia Alina
Virla, Eduardo Gabriel
Mccarthy, Cristina Beryl
author Rozadilla, Gastón
author_facet Rozadilla, Gastón
Cabrera, Natalia Alina
Virla, Eduardo Gabriel
Mccarthy, Cristina Beryl
author_role author
author2 Cabrera, Natalia Alina
Virla, Eduardo Gabriel
Mccarthy, Cristina Beryl
author2_role author
author
author
dc.subject.none.fl_str_mv METATRASCRIPTOMICS
SPODOPTERA
MAIZE
MICROBIOTA
topic METATRASCRIPTOMICS
SPODOPTERA
MAIZE
MICROBIOTA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Spodoptera frugiperda is a noctuid moth that devastates various crops, including corn, and is found in most of the American continent. Transgenic crops that produce insecticidal proteins from Bacillus thuringiensis are currently the most successful biotechnological pest management application, but S. frugiperda has developed field-evolved resistance. In this regard, insect gut microbiota conform a complex community that establishes symbiotic relationships with its host, contributing to its viability. For this reason, metatranscriptomic and transcriptomic analyses of insect guts in their natural environment are invaluable to better comprehend their biology and to identify genes as targets for pest control. We previously captured S. frugiperda specimens from different environments, altitudes and food sources in the province of Tucumán (Argentina). For all samples, total RNA extracted from fifth instar larval guts was submitted to a one-step reverse transcription and PCR sequence independent amplification procedure, and then pyrosequenced. In this study we analysed one of these samples, namely, larvae that were captured in an organic maize field in Tafí del Valle (26°55´40.75´´S, 65°45´19.90´´W; Tucumán province) at 2283 meters above sea level. Sequence reads were trimmed and assembled. Homology searches were performed against various NCBI databases. Taxonomic and functional contents were analysed with MEGAN. The metatranscriptome, in which we identified sequences from archaea, bacteria, fungi, nematodes and plants, revealed potential biocontrol candidates for this pest, and others related with host metabolism and digestion. Furthermore, the host transcriptome showed that most transcripts were associated with the digestive tract structure and development, among others. Some of these genes could be possible targets for pest control via RNA interference (RNAi). In summary, this study has shown the potential effects of this particular food source (i.e., organic maize) and of the environmental conditions (altitude, among others), on the expression profile of S. frugiperda larval guts, their associated metatranscriptome, and putative interactions between them. Future studies will test the potential biocontrol candidates that we identified.
Fil: Rozadilla, Gastón. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cabrera, Natalia Alina. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina
Fil: Virla, Eduardo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Mccarthy, Cristina Beryl. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina
X Congreso Argentino de Entomología
Mendoza
Argentina
Sociedad Argentina de Entomología
Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias
Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas
Instituto Nacional de Tecnología Agropecuaria
description Spodoptera frugiperda is a noctuid moth that devastates various crops, including corn, and is found in most of the American continent. Transgenic crops that produce insecticidal proteins from Bacillus thuringiensis are currently the most successful biotechnological pest management application, but S. frugiperda has developed field-evolved resistance. In this regard, insect gut microbiota conform a complex community that establishes symbiotic relationships with its host, contributing to its viability. For this reason, metatranscriptomic and transcriptomic analyses of insect guts in their natural environment are invaluable to better comprehend their biology and to identify genes as targets for pest control. We previously captured S. frugiperda specimens from different environments, altitudes and food sources in the province of Tucumán (Argentina). For all samples, total RNA extracted from fifth instar larval guts was submitted to a one-step reverse transcription and PCR sequence independent amplification procedure, and then pyrosequenced. In this study we analysed one of these samples, namely, larvae that were captured in an organic maize field in Tafí del Valle (26°55´40.75´´S, 65°45´19.90´´W; Tucumán province) at 2283 meters above sea level. Sequence reads were trimmed and assembled. Homology searches were performed against various NCBI databases. Taxonomic and functional contents were analysed with MEGAN. The metatranscriptome, in which we identified sequences from archaea, bacteria, fungi, nematodes and plants, revealed potential biocontrol candidates for this pest, and others related with host metabolism and digestion. Furthermore, the host transcriptome showed that most transcripts were associated with the digestive tract structure and development, among others. Some of these genes could be possible targets for pest control via RNA interference (RNAi). In summary, this study has shown the potential effects of this particular food source (i.e., organic maize) and of the environmental conditions (altitude, among others), on the expression profile of S. frugiperda larval guts, their associated metatranscriptome, and putative interactions between them. Future studies will test the potential biocontrol candidates that we identified.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/187930
Inside the gut: Revelations of metatranscriptomic and transcriptomic analyses of Spodoptera frugiperda larvae from an organic maize plantation at 2283 meters above sea level (Tafí del Valle, Tucumán); X Congreso Argentino de Entomología; Mendoza; Argentina; 2018; 1-2
978-987-575-179-8
CONICET Digital
CONICET
url http://hdl.handle.net/11336/187930
identifier_str_mv Inside the gut: Revelations of metatranscriptomic and transcriptomic analyses of Spodoptera frugiperda larvae from an organic maize plantation at 2283 meters above sea level (Tafí del Valle, Tucumán); X Congreso Argentino de Entomología; Mendoza; Argentina; 2018; 1-2
978-987-575-179-8
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://xcaeorg.files.wordpress.com/2018/06/libro-de-resumenes-xcae-2018.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Universidad Nacional de Cuyo
publisher.none.fl_str_mv Universidad Nacional de Cuyo
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614516153778176
score 13.070432

Publicaciones similares