The low-abundance transcriptome reveals novel biomarkers, specific intracellular pathways and targetable genes associated with advanced gastric cancer
- Autores
- Bizama, Carolina; Benavente, Felipe; Salvatierra, Edgardo; Gutiérrez Moraga, Ana; Espinoza, Jaime A.; Fernandez, Elmer Andres; Roa, Iván; Mazzolini Rizzo, Guillermo Daniel; Sagredo, Eduardo A.; Gidekel, Manuel; Podhajcer, Osvaldo Luis
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Studies on the low-abundance transcriptome are of paramount importance for identifying the intimate mechanisms of tumor progression that can lead to novel therapies. The aim of the present study was to identify novel markers and targetable genes and pathways in advanced human gastric cancer through analyses of the low-abundance transcriptome. The procedure involved an initial subtractive hybridization step, followed by global gene expression analysis using microarrays. We observed profound differences, both at the single gene and gene ontology levels, between the low-abundance transcriptome and the whole transcriptome. Analysis of the low-abundance transcriptome led to the identification and validation by tissue microarrays of novel biomarkers, such as LAMA3 and TTN; moreover, we identified cancer type-specific intracellular pathways and targetable genes, such as IRS2, IL17, IFNγ, VEGF-C, WISP1, FZD5 and CTBP1 that were not detectable by whole transcriptome analyses. We also demonstrated that knocking down the expression of CTBP1 sensitized gastric cancer cells to mainstay chemotherapeutic drugs. We conclude that the analysis of the low-abundance transcriptome provides useful insights into the molecular basis and treatment of cancer.
Fil: Bizama, Carolina. Universidad de la Frontera; Chile
Fil: Benavente, Felipe. Universidad de la Frontera; Chile
Fil: Salvatierra, Edgardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Gutiérrez Moraga, Ana. Universidad de la Frontera; Chile
Fil: Espinoza, Jaime A.. Universidad de la Frontera; Chile
Fil: Fernandez, Elmer Andres. Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Roa, Iván. Creative BioScience; Chile
Fil: Mazzolini Rizzo, Guillermo Daniel. Universidad Austral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sagredo, Eduardo A.. Universidad de la Frontera; Chile
Fil: Gidekel, Manuel. Universidad de la Frontera; Chile
Fil: Podhajcer, Osvaldo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina - Materia
-
Gastric And Colon Cancer
Novel Biomarkers And Therapeutic Targets
Low-Abundance Transcriptome
Chemotherapy Sensitization - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/19654
Ver los metadatos del registro completo
id |
CONICETDig_a9900397ede2f4d34e9416a4279d1dd5 |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/19654 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
The low-abundance transcriptome reveals novel biomarkers, specific intracellular pathways and targetable genes associated with advanced gastric cancerBizama, CarolinaBenavente, FelipeSalvatierra, EdgardoGutiérrez Moraga, AnaEspinoza, Jaime A.Fernandez, Elmer AndresRoa, IvánMazzolini Rizzo, Guillermo DanielSagredo, Eduardo A.Gidekel, ManuelPodhajcer, Osvaldo LuisGastric And Colon CancerNovel Biomarkers And Therapeutic TargetsLow-Abundance TranscriptomeChemotherapy Sensitizationhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Studies on the low-abundance transcriptome are of paramount importance for identifying the intimate mechanisms of tumor progression that can lead to novel therapies. The aim of the present study was to identify novel markers and targetable genes and pathways in advanced human gastric cancer through analyses of the low-abundance transcriptome. The procedure involved an initial subtractive hybridization step, followed by global gene expression analysis using microarrays. We observed profound differences, both at the single gene and gene ontology levels, between the low-abundance transcriptome and the whole transcriptome. Analysis of the low-abundance transcriptome led to the identification and validation by tissue microarrays of novel biomarkers, such as LAMA3 and TTN; moreover, we identified cancer type-specific intracellular pathways and targetable genes, such as IRS2, IL17, IFNγ, VEGF-C, WISP1, FZD5 and CTBP1 that were not detectable by whole transcriptome analyses. We also demonstrated that knocking down the expression of CTBP1 sensitized gastric cancer cells to mainstay chemotherapeutic drugs. We conclude that the analysis of the low-abundance transcriptome provides useful insights into the molecular basis and treatment of cancer.Fil: Bizama, Carolina. Universidad de la Frontera; ChileFil: Benavente, Felipe. Universidad de la Frontera; ChileFil: Salvatierra, Edgardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Gutiérrez Moraga, Ana. Universidad de la Frontera; ChileFil: Espinoza, Jaime A.. Universidad de la Frontera; ChileFil: Fernandez, Elmer Andres. Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Roa, Iván. Creative BioScience; ChileFil: Mazzolini Rizzo, Guillermo Daniel. Universidad Austral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sagredo, Eduardo A.. Universidad de la Frontera; ChileFil: Gidekel, Manuel. Universidad de la Frontera; ChileFil: Podhajcer, Osvaldo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaWiley2014-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/19654Bizama, Carolina; Benavente, Felipe; Salvatierra, Edgardo; Gutiérrez Moraga, Ana; Espinoza, Jaime A.; et al.; The low-abundance transcriptome reveals novel biomarkers, specific intracellular pathways and targetable genes associated with advanced gastric cancer; Wiley; International Journal Of Cancer. Journal International Du Cancer.; 134; 4; 2-2014; 755-7640020-71361097-0215CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/ijc.28405/fullinfo:eu-repo/semantics/altIdentifier/doi/10.1002/ijc.28405info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:04:37Zoai:ri.conicet.gov.ar:11336/19654instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:04:37.431CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
The low-abundance transcriptome reveals novel biomarkers, specific intracellular pathways and targetable genes associated with advanced gastric cancer |
title |
The low-abundance transcriptome reveals novel biomarkers, specific intracellular pathways and targetable genes associated with advanced gastric cancer |
spellingShingle |
The low-abundance transcriptome reveals novel biomarkers, specific intracellular pathways and targetable genes associated with advanced gastric cancer Bizama, Carolina Gastric And Colon Cancer Novel Biomarkers And Therapeutic Targets Low-Abundance Transcriptome Chemotherapy Sensitization |
title_short |
The low-abundance transcriptome reveals novel biomarkers, specific intracellular pathways and targetable genes associated with advanced gastric cancer |
title_full |
The low-abundance transcriptome reveals novel biomarkers, specific intracellular pathways and targetable genes associated with advanced gastric cancer |
title_fullStr |
The low-abundance transcriptome reveals novel biomarkers, specific intracellular pathways and targetable genes associated with advanced gastric cancer |
title_full_unstemmed |
The low-abundance transcriptome reveals novel biomarkers, specific intracellular pathways and targetable genes associated with advanced gastric cancer |
title_sort |
The low-abundance transcriptome reveals novel biomarkers, specific intracellular pathways and targetable genes associated with advanced gastric cancer |
dc.creator.none.fl_str_mv |
Bizama, Carolina Benavente, Felipe Salvatierra, Edgardo Gutiérrez Moraga, Ana Espinoza, Jaime A. Fernandez, Elmer Andres Roa, Iván Mazzolini Rizzo, Guillermo Daniel Sagredo, Eduardo A. Gidekel, Manuel Podhajcer, Osvaldo Luis |
author |
Bizama, Carolina |
author_facet |
Bizama, Carolina Benavente, Felipe Salvatierra, Edgardo Gutiérrez Moraga, Ana Espinoza, Jaime A. Fernandez, Elmer Andres Roa, Iván Mazzolini Rizzo, Guillermo Daniel Sagredo, Eduardo A. Gidekel, Manuel Podhajcer, Osvaldo Luis |
author_role |
author |
author2 |
Benavente, Felipe Salvatierra, Edgardo Gutiérrez Moraga, Ana Espinoza, Jaime A. Fernandez, Elmer Andres Roa, Iván Mazzolini Rizzo, Guillermo Daniel Sagredo, Eduardo A. Gidekel, Manuel Podhajcer, Osvaldo Luis |
author2_role |
author author author author author author author author author author |
dc.subject.none.fl_str_mv |
Gastric And Colon Cancer Novel Biomarkers And Therapeutic Targets Low-Abundance Transcriptome Chemotherapy Sensitization |
topic |
Gastric And Colon Cancer Novel Biomarkers And Therapeutic Targets Low-Abundance Transcriptome Chemotherapy Sensitization |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Studies on the low-abundance transcriptome are of paramount importance for identifying the intimate mechanisms of tumor progression that can lead to novel therapies. The aim of the present study was to identify novel markers and targetable genes and pathways in advanced human gastric cancer through analyses of the low-abundance transcriptome. The procedure involved an initial subtractive hybridization step, followed by global gene expression analysis using microarrays. We observed profound differences, both at the single gene and gene ontology levels, between the low-abundance transcriptome and the whole transcriptome. Analysis of the low-abundance transcriptome led to the identification and validation by tissue microarrays of novel biomarkers, such as LAMA3 and TTN; moreover, we identified cancer type-specific intracellular pathways and targetable genes, such as IRS2, IL17, IFNγ, VEGF-C, WISP1, FZD5 and CTBP1 that were not detectable by whole transcriptome analyses. We also demonstrated that knocking down the expression of CTBP1 sensitized gastric cancer cells to mainstay chemotherapeutic drugs. We conclude that the analysis of the low-abundance transcriptome provides useful insights into the molecular basis and treatment of cancer. Fil: Bizama, Carolina. Universidad de la Frontera; Chile Fil: Benavente, Felipe. Universidad de la Frontera; Chile Fil: Salvatierra, Edgardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina Fil: Gutiérrez Moraga, Ana. Universidad de la Frontera; Chile Fil: Espinoza, Jaime A.. Universidad de la Frontera; Chile Fil: Fernandez, Elmer Andres. Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Roa, Iván. Creative BioScience; Chile Fil: Mazzolini Rizzo, Guillermo Daniel. Universidad Austral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Sagredo, Eduardo A.. Universidad de la Frontera; Chile Fil: Gidekel, Manuel. Universidad de la Frontera; Chile Fil: Podhajcer, Osvaldo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina |
description |
Studies on the low-abundance transcriptome are of paramount importance for identifying the intimate mechanisms of tumor progression that can lead to novel therapies. The aim of the present study was to identify novel markers and targetable genes and pathways in advanced human gastric cancer through analyses of the low-abundance transcriptome. The procedure involved an initial subtractive hybridization step, followed by global gene expression analysis using microarrays. We observed profound differences, both at the single gene and gene ontology levels, between the low-abundance transcriptome and the whole transcriptome. Analysis of the low-abundance transcriptome led to the identification and validation by tissue microarrays of novel biomarkers, such as LAMA3 and TTN; moreover, we identified cancer type-specific intracellular pathways and targetable genes, such as IRS2, IL17, IFNγ, VEGF-C, WISP1, FZD5 and CTBP1 that were not detectable by whole transcriptome analyses. We also demonstrated that knocking down the expression of CTBP1 sensitized gastric cancer cells to mainstay chemotherapeutic drugs. We conclude that the analysis of the low-abundance transcriptome provides useful insights into the molecular basis and treatment of cancer. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-02 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/19654 Bizama, Carolina; Benavente, Felipe; Salvatierra, Edgardo; Gutiérrez Moraga, Ana; Espinoza, Jaime A.; et al.; The low-abundance transcriptome reveals novel biomarkers, specific intracellular pathways and targetable genes associated with advanced gastric cancer; Wiley; International Journal Of Cancer. Journal International Du Cancer.; 134; 4; 2-2014; 755-764 0020-7136 1097-0215 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/19654 |
identifier_str_mv |
Bizama, Carolina; Benavente, Felipe; Salvatierra, Edgardo; Gutiérrez Moraga, Ana; Espinoza, Jaime A.; et al.; The low-abundance transcriptome reveals novel biomarkers, specific intracellular pathways and targetable genes associated with advanced gastric cancer; Wiley; International Journal Of Cancer. Journal International Du Cancer.; 134; 4; 2-2014; 755-764 0020-7136 1097-0215 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/ijc.28405/full info:eu-repo/semantics/altIdentifier/doi/10.1002/ijc.28405 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844613873775149056 |
score |
13.070432 |