The low-abundance transcriptome reveals novel biomarkers, specific intracellular pathways and targetable genes associated with advanced gastric cancer

Autores
Bizama, Carolina; Benavente, Felipe; Salvatierra, Edgardo; Gutiérrez Moraga, Ana; Espinoza, Jaime A.; Fernandez, Elmer Andres; Roa, Iván; Mazzolini Rizzo, Guillermo Daniel; Sagredo, Eduardo A.; Gidekel, Manuel; Podhajcer, Osvaldo Luis
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Studies on the low-abundance transcriptome are of paramount importance for identifying the intimate mechanisms of tumor progression that can lead to novel therapies. The aim of the present study was to identify novel markers and targetable genes and pathways in advanced human gastric cancer through analyses of the low-abundance transcriptome. The procedure involved an initial subtractive hybridization step, followed by global gene expression analysis using microarrays. We observed profound differences, both at the single gene and gene ontology levels, between the low-abundance transcriptome and the whole transcriptome. Analysis of the low-abundance transcriptome led to the identification and validation by tissue microarrays of novel biomarkers, such as LAMA3 and TTN; moreover, we identified cancer type-specific intracellular pathways and targetable genes, such as IRS2, IL17, IFNγ, VEGF-C, WISP1, FZD5 and CTBP1 that were not detectable by whole transcriptome analyses. We also demonstrated that knocking down the expression of CTBP1 sensitized gastric cancer cells to mainstay chemotherapeutic drugs. We conclude that the analysis of the low-abundance transcriptome provides useful insights into the molecular basis and treatment of cancer.
Fil: Bizama, Carolina. Universidad de la Frontera; Chile
Fil: Benavente, Felipe. Universidad de la Frontera; Chile
Fil: Salvatierra, Edgardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Gutiérrez Moraga, Ana. Universidad de la Frontera; Chile
Fil: Espinoza, Jaime A.. Universidad de la Frontera; Chile
Fil: Fernandez, Elmer Andres. Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Roa, Iván. Creative BioScience; Chile
Fil: Mazzolini Rizzo, Guillermo Daniel. Universidad Austral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sagredo, Eduardo A.. Universidad de la Frontera; Chile
Fil: Gidekel, Manuel. Universidad de la Frontera; Chile
Fil: Podhajcer, Osvaldo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Materia
Gastric And Colon Cancer
Novel Biomarkers And Therapeutic Targets
Low-Abundance Transcriptome
Chemotherapy Sensitization
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/19654
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/19654
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The low-abundance transcriptome reveals novel biomarkers, specific intracellular pathways and targetable genes associated with advanced gastric cancerBizama, CarolinaBenavente, FelipeSalvatierra, EdgardoGutiérrez Moraga, AnaEspinoza, Jaime A.Fernandez, Elmer AndresRoa, IvánMazzolini Rizzo, Guillermo DanielSagredo, Eduardo A.Gidekel, ManuelPodhajcer, Osvaldo LuisGastric And Colon CancerNovel Biomarkers And Therapeutic TargetsLow-Abundance TranscriptomeChemotherapy Sensitizationhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Studies on the low-abundance transcriptome are of paramount importance for identifying the intimate mechanisms of tumor progression that can lead to novel therapies. The aim of the present study was to identify novel markers and targetable genes and pathways in advanced human gastric cancer through analyses of the low-abundance transcriptome. The procedure involved an initial subtractive hybridization step, followed by global gene expression analysis using microarrays. We observed profound differences, both at the single gene and gene ontology levels, between the low-abundance transcriptome and the whole transcriptome. Analysis of the low-abundance transcriptome led to the identification and validation by tissue microarrays of novel biomarkers, such as LAMA3 and TTN; moreover, we identified cancer type-specific intracellular pathways and targetable genes, such as IRS2, IL17, IFNγ, VEGF-C, WISP1, FZD5 and CTBP1 that were not detectable by whole transcriptome analyses. We also demonstrated that knocking down the expression of CTBP1 sensitized gastric cancer cells to mainstay chemotherapeutic drugs. We conclude that the analysis of the low-abundance transcriptome provides useful insights into the molecular basis and treatment of cancer.Fil: Bizama, Carolina. Universidad de la Frontera; ChileFil: Benavente, Felipe. Universidad de la Frontera; ChileFil: Salvatierra, Edgardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Gutiérrez Moraga, Ana. Universidad de la Frontera; ChileFil: Espinoza, Jaime A.. Universidad de la Frontera; ChileFil: Fernandez, Elmer Andres. Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Roa, Iván. Creative BioScience; ChileFil: Mazzolini Rizzo, Guillermo Daniel. Universidad Austral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sagredo, Eduardo A.. Universidad de la Frontera; ChileFil: Gidekel, Manuel. Universidad de la Frontera; ChileFil: Podhajcer, Osvaldo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaWiley2014-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/19654Bizama, Carolina; Benavente, Felipe; Salvatierra, Edgardo; Gutiérrez Moraga, Ana; Espinoza, Jaime A.; et al.; The low-abundance transcriptome reveals novel biomarkers, specific intracellular pathways and targetable genes associated with advanced gastric cancer; Wiley; International Journal Of Cancer. Journal International Du Cancer.; 134; 4; 2-2014; 755-7640020-71361097-0215CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/ijc.28405/fullinfo:eu-repo/semantics/altIdentifier/doi/10.1002/ijc.28405info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:34:21Zoai:ri.conicet.gov.ar:11336/19654instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:34:22.258CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The low-abundance transcriptome reveals novel biomarkers, specific intracellular pathways and targetable genes associated with advanced gastric cancer
title The low-abundance transcriptome reveals novel biomarkers, specific intracellular pathways and targetable genes associated with advanced gastric cancer
spellingShingle The low-abundance transcriptome reveals novel biomarkers, specific intracellular pathways and targetable genes associated with advanced gastric cancer
Bizama, Carolina
Gastric And Colon Cancer
Novel Biomarkers And Therapeutic Targets
Low-Abundance Transcriptome
Chemotherapy Sensitization
title_short The low-abundance transcriptome reveals novel biomarkers, specific intracellular pathways and targetable genes associated with advanced gastric cancer
title_full The low-abundance transcriptome reveals novel biomarkers, specific intracellular pathways and targetable genes associated with advanced gastric cancer
title_fullStr The low-abundance transcriptome reveals novel biomarkers, specific intracellular pathways and targetable genes associated with advanced gastric cancer
title_full_unstemmed The low-abundance transcriptome reveals novel biomarkers, specific intracellular pathways and targetable genes associated with advanced gastric cancer
title_sort The low-abundance transcriptome reveals novel biomarkers, specific intracellular pathways and targetable genes associated with advanced gastric cancer
dc.creator.none.fl_str_mv Bizama, Carolina
Benavente, Felipe
Salvatierra, Edgardo
Gutiérrez Moraga, Ana
Espinoza, Jaime A.
Fernandez, Elmer Andres
Roa, Iván
Mazzolini Rizzo, Guillermo Daniel
Sagredo, Eduardo A.
Gidekel, Manuel
Podhajcer, Osvaldo Luis
author Bizama, Carolina
author_facet Bizama, Carolina
Benavente, Felipe
Salvatierra, Edgardo
Gutiérrez Moraga, Ana
Espinoza, Jaime A.
Fernandez, Elmer Andres
Roa, Iván
Mazzolini Rizzo, Guillermo Daniel
Sagredo, Eduardo A.
Gidekel, Manuel
Podhajcer, Osvaldo Luis
author_role author
author2 Benavente, Felipe
Salvatierra, Edgardo
Gutiérrez Moraga, Ana
Espinoza, Jaime A.
Fernandez, Elmer Andres
Roa, Iván
Mazzolini Rizzo, Guillermo Daniel
Sagredo, Eduardo A.
Gidekel, Manuel
Podhajcer, Osvaldo Luis
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Gastric And Colon Cancer
Novel Biomarkers And Therapeutic Targets
Low-Abundance Transcriptome
Chemotherapy Sensitization
topic Gastric And Colon Cancer
Novel Biomarkers And Therapeutic Targets
Low-Abundance Transcriptome
Chemotherapy Sensitization
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Studies on the low-abundance transcriptome are of paramount importance for identifying the intimate mechanisms of tumor progression that can lead to novel therapies. The aim of the present study was to identify novel markers and targetable genes and pathways in advanced human gastric cancer through analyses of the low-abundance transcriptome. The procedure involved an initial subtractive hybridization step, followed by global gene expression analysis using microarrays. We observed profound differences, both at the single gene and gene ontology levels, between the low-abundance transcriptome and the whole transcriptome. Analysis of the low-abundance transcriptome led to the identification and validation by tissue microarrays of novel biomarkers, such as LAMA3 and TTN; moreover, we identified cancer type-specific intracellular pathways and targetable genes, such as IRS2, IL17, IFNγ, VEGF-C, WISP1, FZD5 and CTBP1 that were not detectable by whole transcriptome analyses. We also demonstrated that knocking down the expression of CTBP1 sensitized gastric cancer cells to mainstay chemotherapeutic drugs. We conclude that the analysis of the low-abundance transcriptome provides useful insights into the molecular basis and treatment of cancer.
Fil: Bizama, Carolina. Universidad de la Frontera; Chile
Fil: Benavente, Felipe. Universidad de la Frontera; Chile
Fil: Salvatierra, Edgardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Gutiérrez Moraga, Ana. Universidad de la Frontera; Chile
Fil: Espinoza, Jaime A.. Universidad de la Frontera; Chile
Fil: Fernandez, Elmer Andres. Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Roa, Iván. Creative BioScience; Chile
Fil: Mazzolini Rizzo, Guillermo Daniel. Universidad Austral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sagredo, Eduardo A.. Universidad de la Frontera; Chile
Fil: Gidekel, Manuel. Universidad de la Frontera; Chile
Fil: Podhajcer, Osvaldo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
description Studies on the low-abundance transcriptome are of paramount importance for identifying the intimate mechanisms of tumor progression that can lead to novel therapies. The aim of the present study was to identify novel markers and targetable genes and pathways in advanced human gastric cancer through analyses of the low-abundance transcriptome. The procedure involved an initial subtractive hybridization step, followed by global gene expression analysis using microarrays. We observed profound differences, both at the single gene and gene ontology levels, between the low-abundance transcriptome and the whole transcriptome. Analysis of the low-abundance transcriptome led to the identification and validation by tissue microarrays of novel biomarkers, such as LAMA3 and TTN; moreover, we identified cancer type-specific intracellular pathways and targetable genes, such as IRS2, IL17, IFNγ, VEGF-C, WISP1, FZD5 and CTBP1 that were not detectable by whole transcriptome analyses. We also demonstrated that knocking down the expression of CTBP1 sensitized gastric cancer cells to mainstay chemotherapeutic drugs. We conclude that the analysis of the low-abundance transcriptome provides useful insights into the molecular basis and treatment of cancer.
publishDate 2014
dc.date.none.fl_str_mv 2014-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/19654
Bizama, Carolina; Benavente, Felipe; Salvatierra, Edgardo; Gutiérrez Moraga, Ana; Espinoza, Jaime A.; et al.; The low-abundance transcriptome reveals novel biomarkers, specific intracellular pathways and targetable genes associated with advanced gastric cancer; Wiley; International Journal Of Cancer. Journal International Du Cancer.; 134; 4; 2-2014; 755-764
0020-7136
1097-0215
CONICET Digital
CONICET
url http://hdl.handle.net/11336/19654
identifier_str_mv Bizama, Carolina; Benavente, Felipe; Salvatierra, Edgardo; Gutiérrez Moraga, Ana; Espinoza, Jaime A.; et al.; The low-abundance transcriptome reveals novel biomarkers, specific intracellular pathways and targetable genes associated with advanced gastric cancer; Wiley; International Journal Of Cancer. Journal International Du Cancer.; 134; 4; 2-2014; 755-764
0020-7136
1097-0215
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/ijc.28405/full
info:eu-repo/semantics/altIdentifier/doi/10.1002/ijc.28405
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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