Recombinant Glutathione S-Transferase adsorbed to aluminum hydroxide: A vaccine candidate against Fasciola hepatica in mice

Autores
Fernandez, Vanesa; Solana, Hugo Daniel; Ortiz, Pedro; Dominguez, Maria Paula; Estein, Silvia Marcela
Año de publicación
2017
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Fasciolosis is a parasitic zoonosis caused by infection with Fasciola hepatica. Disease control using Triclabendazole (TCBZ) results in the development of anthelmintic resistance against the drug. Vaccination would be an attractive option to pursue in fasciolosis control to reduce the need for anthelmintics. We evaluated the immunogenicity and protection conferred by a recombinant Glutathione-S Transferase- Mu (rFhGSTMu) protein against F. hepatica in mice. The recombinant enzyme was produced in Escherichia coli. IgG and IgG subisotypes were measured using an ELISA. Liver damage was estimated by the determination of serum Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT) and Alkaline Phosphatase (AP) activity. Balb/c mice were distributed across four groups (n=10/group) immunized subcutaneously at weeks 0, 2 and 4 as follows: Group 1: rFhGSTMu + Freund Incomplete Adjuvant (FIA); Group 2: rFhGSTMu + Aluminum Hydroxide (AH), Group 3: rFhGSTMu + Quil A and Group 4 (control group) was injected with saline. All groups were challenged two weeks after the last immunization with six metacercariae of F. hepatica. All vaccine formulations induced IgG specific antibodies with a mixed IgG1/IgG2a response. rFhGSTMu + AH induced significant reduction in worm counts (90% ). Other formulations, however did not induce a significant reduction in worm counts (0 to 10% similar to the unvaccinated control group). Liver enzyme activities in the group immunized with rFhGSTMu + AH were significantly lower than values recorded in the other groups. Our results indicated that rFhGSTMu formulated in AH is a potential vaccine candidate against F. hepatica in the mouse model.
Fil: Fernandez, Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Solana, Hugo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Ortiz, Pedro. Universidad Nacional de Cajamarca; Perú
Fil: Dominguez, Maria Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Estein, Silvia Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
26th International Conference of the World Association for the Advancement of Veterinary Pharasitology
Kuala Lumpur
Malasia
World Association for the Advancement of Veterinary Pharasitology
Materia
FASCIOLA HEPATICA
MICE
VACCINE
GLUTATION S TRANSFERASA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/182426
Acceder
id CONICETDig_a8e6b392366ab6288dacb76fc584c7d3
oai_identifier_str oai:ri.conicet.gov.ar:11336/182426
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Recombinant Glutathione S-Transferase adsorbed to aluminum hydroxide: A vaccine candidate against Fasciola hepatica in miceFernandez, VanesaSolana, Hugo DanielOrtiz, PedroDominguez, Maria PaulaEstein, Silvia MarcelaFASCIOLA HEPATICAMICEVACCINEGLUTATION S TRANSFERASAhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Fasciolosis is a parasitic zoonosis caused by infection with Fasciola hepatica. Disease control using Triclabendazole (TCBZ) results in the development of anthelmintic resistance against the drug. Vaccination would be an attractive option to pursue in fasciolosis control to reduce the need for anthelmintics. We evaluated the immunogenicity and protection conferred by a recombinant Glutathione-S Transferase- Mu (rFhGSTMu) protein against F. hepatica in mice. The recombinant enzyme was produced in Escherichia coli. IgG and IgG subisotypes were measured using an ELISA. Liver damage was estimated by the determination of serum Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT) and Alkaline Phosphatase (AP) activity. Balb/c mice were distributed across four groups (n=10/group) immunized subcutaneously at weeks 0, 2 and 4 as follows: Group 1: rFhGSTMu + Freund Incomplete Adjuvant (FIA); Group 2: rFhGSTMu + Aluminum Hydroxide (AH), Group 3: rFhGSTMu + Quil A and Group 4 (control group) was injected with saline. All groups were challenged two weeks after the last immunization with six metacercariae of F. hepatica. All vaccine formulations induced IgG specific antibodies with a mixed IgG1/IgG2a response. rFhGSTMu + AH induced significant reduction in worm counts (90% ). Other formulations, however did not induce a significant reduction in worm counts (0 to 10% similar to the unvaccinated control group). Liver enzyme activities in the group immunized with rFhGSTMu + AH were significantly lower than values recorded in the other groups. Our results indicated that rFhGSTMu formulated in AH is a potential vaccine candidate against F. hepatica in the mouse model.Fil: Fernandez, Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Solana, Hugo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Ortiz, Pedro. Universidad Nacional de Cajamarca; PerúFil: Dominguez, Maria Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Estein, Silvia Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina26th International Conference of the World Association for the Advancement of Veterinary PharasitologyKuala LumpurMalasiaWorld Association for the Advancement of Veterinary PharasitologyWorld Association for the Advancement of Veterinary Pharasitology2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectConferenciaBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/182426Recombinant Glutathione S-Transferase adsorbed to aluminum hydroxide: A vaccine candidate against Fasciola hepatica in mice; 26th International Conference of the World Association for the Advancement of Veterinary Pharasitology; Kuala Lumpur; Malasia; 2017; 462CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://ir.unimas.my/id/eprint/18437/1/WAAVP%202017%20Abstract%20Book.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:48:41Zoai:ri.conicet.gov.ar:11336/182426instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:48:42.268CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Recombinant Glutathione S-Transferase adsorbed to aluminum hydroxide: A vaccine candidate against Fasciola hepatica in mice
title Recombinant Glutathione S-Transferase adsorbed to aluminum hydroxide: A vaccine candidate against Fasciola hepatica in mice
spellingShingle Recombinant Glutathione S-Transferase adsorbed to aluminum hydroxide: A vaccine candidate against Fasciola hepatica in mice
Fernandez, Vanesa
FASCIOLA HEPATICA
MICE
VACCINE
GLUTATION S TRANSFERASA
title_short Recombinant Glutathione S-Transferase adsorbed to aluminum hydroxide: A vaccine candidate against Fasciola hepatica in mice
title_full Recombinant Glutathione S-Transferase adsorbed to aluminum hydroxide: A vaccine candidate against Fasciola hepatica in mice
title_fullStr Recombinant Glutathione S-Transferase adsorbed to aluminum hydroxide: A vaccine candidate against Fasciola hepatica in mice
title_full_unstemmed Recombinant Glutathione S-Transferase adsorbed to aluminum hydroxide: A vaccine candidate against Fasciola hepatica in mice
title_sort Recombinant Glutathione S-Transferase adsorbed to aluminum hydroxide: A vaccine candidate against Fasciola hepatica in mice
dc.creator.none.fl_str_mv Fernandez, Vanesa
Solana, Hugo Daniel
Ortiz, Pedro
Dominguez, Maria Paula
Estein, Silvia Marcela
author Fernandez, Vanesa
author_facet Fernandez, Vanesa
Solana, Hugo Daniel
Ortiz, Pedro
Dominguez, Maria Paula
Estein, Silvia Marcela
author_role author
author2 Solana, Hugo Daniel
Ortiz, Pedro
Dominguez, Maria Paula
Estein, Silvia Marcela
author2_role author
author
author
author
dc.subject.none.fl_str_mv FASCIOLA HEPATICA
MICE
VACCINE
GLUTATION S TRANSFERASA
topic FASCIOLA HEPATICA
MICE
VACCINE
GLUTATION S TRANSFERASA
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Fasciolosis is a parasitic zoonosis caused by infection with Fasciola hepatica. Disease control using Triclabendazole (TCBZ) results in the development of anthelmintic resistance against the drug. Vaccination would be an attractive option to pursue in fasciolosis control to reduce the need for anthelmintics. We evaluated the immunogenicity and protection conferred by a recombinant Glutathione-S Transferase- Mu (rFhGSTMu) protein against F. hepatica in mice. The recombinant enzyme was produced in Escherichia coli. IgG and IgG subisotypes were measured using an ELISA. Liver damage was estimated by the determination of serum Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT) and Alkaline Phosphatase (AP) activity. Balb/c mice were distributed across four groups (n=10/group) immunized subcutaneously at weeks 0, 2 and 4 as follows: Group 1: rFhGSTMu + Freund Incomplete Adjuvant (FIA); Group 2: rFhGSTMu + Aluminum Hydroxide (AH), Group 3: rFhGSTMu + Quil A and Group 4 (control group) was injected with saline. All groups were challenged two weeks after the last immunization with six metacercariae of F. hepatica. All vaccine formulations induced IgG specific antibodies with a mixed IgG1/IgG2a response. rFhGSTMu + AH induced significant reduction in worm counts (90% ). Other formulations, however did not induce a significant reduction in worm counts (0 to 10% similar to the unvaccinated control group). Liver enzyme activities in the group immunized with rFhGSTMu + AH were significantly lower than values recorded in the other groups. Our results indicated that rFhGSTMu formulated in AH is a potential vaccine candidate against F. hepatica in the mouse model.
Fil: Fernandez, Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Solana, Hugo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Ortiz, Pedro. Universidad Nacional de Cajamarca; Perú
Fil: Dominguez, Maria Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Estein, Silvia Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
26th International Conference of the World Association for the Advancement of Veterinary Pharasitology
Kuala Lumpur
Malasia
World Association for the Advancement of Veterinary Pharasitology
description Fasciolosis is a parasitic zoonosis caused by infection with Fasciola hepatica. Disease control using Triclabendazole (TCBZ) results in the development of anthelmintic resistance against the drug. Vaccination would be an attractive option to pursue in fasciolosis control to reduce the need for anthelmintics. We evaluated the immunogenicity and protection conferred by a recombinant Glutathione-S Transferase- Mu (rFhGSTMu) protein against F. hepatica in mice. The recombinant enzyme was produced in Escherichia coli. IgG and IgG subisotypes were measured using an ELISA. Liver damage was estimated by the determination of serum Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT) and Alkaline Phosphatase (AP) activity. Balb/c mice were distributed across four groups (n=10/group) immunized subcutaneously at weeks 0, 2 and 4 as follows: Group 1: rFhGSTMu + Freund Incomplete Adjuvant (FIA); Group 2: rFhGSTMu + Aluminum Hydroxide (AH), Group 3: rFhGSTMu + Quil A and Group 4 (control group) was injected with saline. All groups were challenged two weeks after the last immunization with six metacercariae of F. hepatica. All vaccine formulations induced IgG specific antibodies with a mixed IgG1/IgG2a response. rFhGSTMu + AH induced significant reduction in worm counts (90% ). Other formulations, however did not induce a significant reduction in worm counts (0 to 10% similar to the unvaccinated control group). Liver enzyme activities in the group immunized with rFhGSTMu + AH were significantly lower than values recorded in the other groups. Our results indicated that rFhGSTMu formulated in AH is a potential vaccine candidate against F. hepatica in the mouse model.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Conferencia
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/182426
Recombinant Glutathione S-Transferase adsorbed to aluminum hydroxide: A vaccine candidate against Fasciola hepatica in mice; 26th International Conference of the World Association for the Advancement of Veterinary Pharasitology; Kuala Lumpur; Malasia; 2017; 462
CONICET Digital
CONICET
url http://hdl.handle.net/11336/182426
identifier_str_mv Recombinant Glutathione S-Transferase adsorbed to aluminum hydroxide: A vaccine candidate against Fasciola hepatica in mice; 26th International Conference of the World Association for the Advancement of Veterinary Pharasitology; Kuala Lumpur; Malasia; 2017; 462
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://ir.unimas.my/id/eprint/18437/1/WAAVP%202017%20Abstract%20Book.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/vnd.openxmlformats-officedocument.wordprocessingml.document
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv World Association for the Advancement of Veterinary Pharasitology
publisher.none.fl_str_mv World Association for the Advancement of Veterinary Pharasitology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268935350648832
score 13.13397

Publicaciones similares