Galectin-1 is essential for efficient liver regeneration following hepatectomy

Autores
Potikha, Tamara; Ella, Ezra; Cerliani, Juan Pablo; Mizrahi, Lina; Pappo, Orit; Rabinovich, Gabriel Adrián; Galun, Eithan; Goldenberg, Daniel S.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Galectin-1 (Gal1) is a known immune/inflammatory regulator which actsboth extracellularly and intracellularly, modulating innate and adaptive immuneresponses. Here, we explored the role of Gal1 in liver regeneration using 70% partial hepatectomy (PHx) of C57BL/6 wild type and Gal1-knockout (Gal1-KO, Lgals1-/-) mice. Gene or protein expression, in liver samples collected at time intervals from 2 to 168 hours post-operation, was tested by either RT-PCR or by immunoblotting and immunohistochemistry, respectively. We demonstrated that Gal1 transcript and protein expression was induced in the liver tissue of wild type mice upon PHx. Liver regeneration following PHx was significantly delayed in the Gal1-KO compared to the control liver. This delay was accompanied by a decreased Akt phosphorylation, and accumulation of the hepatocyte nuclear p21 protein in the Gal1-KO versus control livers at 24 and 48 hours following PHx. Transcripts of several known regulators of inflammation, cell cycle and cell signaling, including some known PHx-induced genes, were aberrantly expressed (mainly down-regulated) in Gal1-KO compared to control livers at 2, 6 and 24 hours post-PHx. Transient steatosis, which is imperative for liver regeneration following PHx, was significantly delayed and decreased in the Gal1- KO compared to the control liver and was accompanied by a significantly decreased expression in the mutant liver of several genes encoding lipid metabolism regulators.Our results demonstrate that Gal1 protein is essential for efficient liver regeneration following PHx through the regulation of liver inflammation, hepatic cell proliferation, and the control of lipid storage in the regenerating liver.
Fil: Potikha, Tamara. Hadassah Hebrew University Medical Center; Israel
Fil: Ella, Ezra. Hadassah Hebrew University Medical Center; Israel
Fil: Cerliani, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Mizrahi, Lina. Hadassah Hebrew University Medical Center; Israel
Fil: Pappo, Orit. Hadassah Hebrew University Medical Center; Israel
Fil: Rabinovich, Gabriel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Galun, Eithan. Hadassah Hebrew University Medical Center; Israel
Fil: Goldenberg, Daniel S.. Hadassah Hebrew University Medical Center; Israel
Materia
LIVER REGENERATION
GALECTIN-1
HEPATECTOMY
LIPID METABOLISM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/23338
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Galectin-1 is essential for efficient liver regeneration following hepatectomyPotikha, TamaraElla, EzraCerliani, Juan PabloMizrahi, LinaPappo, OritRabinovich, Gabriel AdriánGalun, EithanGoldenberg, Daniel S.LIVER REGENERATIONGALECTIN-1HEPATECTOMYLIPID METABOLISMhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Galectin-1 (Gal1) is a known immune/inflammatory regulator which actsboth extracellularly and intracellularly, modulating innate and adaptive immuneresponses. Here, we explored the role of Gal1 in liver regeneration using 70% partial hepatectomy (PHx) of C57BL/6 wild type and Gal1-knockout (Gal1-KO, Lgals1-/-) mice. Gene or protein expression, in liver samples collected at time intervals from 2 to 168 hours post-operation, was tested by either RT-PCR or by immunoblotting and immunohistochemistry, respectively. We demonstrated that Gal1 transcript and protein expression was induced in the liver tissue of wild type mice upon PHx. Liver regeneration following PHx was significantly delayed in the Gal1-KO compared to the control liver. This delay was accompanied by a decreased Akt phosphorylation, and accumulation of the hepatocyte nuclear p21 protein in the Gal1-KO versus control livers at 24 and 48 hours following PHx. Transcripts of several known regulators of inflammation, cell cycle and cell signaling, including some known PHx-induced genes, were aberrantly expressed (mainly down-regulated) in Gal1-KO compared to control livers at 2, 6 and 24 hours post-PHx. Transient steatosis, which is imperative for liver regeneration following PHx, was significantly delayed and decreased in the Gal1- KO compared to the control liver and was accompanied by a significantly decreased expression in the mutant liver of several genes encoding lipid metabolism regulators.Our results demonstrate that Gal1 protein is essential for efficient liver regeneration following PHx through the regulation of liver inflammation, hepatic cell proliferation, and the control of lipid storage in the regenerating liver.Fil: Potikha, Tamara. Hadassah Hebrew University Medical Center; IsraelFil: Ella, Ezra. Hadassah Hebrew University Medical Center; IsraelFil: Cerliani, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Mizrahi, Lina. Hadassah Hebrew University Medical Center; IsraelFil: Pappo, Orit. Hadassah Hebrew University Medical Center; IsraelFil: Rabinovich, Gabriel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Galun, Eithan. Hadassah Hebrew University Medical Center; IsraelFil: Goldenberg, Daniel S.. Hadassah Hebrew University Medical Center; IsraelImpact journals2016-05-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/23338Potikha, Tamara; Ella, Ezra; Cerliani, Juan Pablo; Mizrahi, Lina; Pappo, Orit; et al.; Galectin-1 is essential for efficient liver regeneration following hepatectomy; Impact journals; Oncotarget; 7; 22; 5-5-2016; 31738-317541949-25531949-2553CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.impactjournals.com/oncotarget/index.php?journal=oncotarget&page=article&op=view&path[]=9194&pubmed-linkout=1info:eu-repo/semantics/altIdentifier/doi/10.18632/oncotarget.9194info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5077973/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:16:13Zoai:ri.conicet.gov.ar:11336/23338instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:16:13.888CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Galectin-1 is essential for efficient liver regeneration following hepatectomy
title Galectin-1 is essential for efficient liver regeneration following hepatectomy
spellingShingle Galectin-1 is essential for efficient liver regeneration following hepatectomy
Potikha, Tamara
LIVER REGENERATION
GALECTIN-1
HEPATECTOMY
LIPID METABOLISM
title_short Galectin-1 is essential for efficient liver regeneration following hepatectomy
title_full Galectin-1 is essential for efficient liver regeneration following hepatectomy
title_fullStr Galectin-1 is essential for efficient liver regeneration following hepatectomy
title_full_unstemmed Galectin-1 is essential for efficient liver regeneration following hepatectomy
title_sort Galectin-1 is essential for efficient liver regeneration following hepatectomy
dc.creator.none.fl_str_mv Potikha, Tamara
Ella, Ezra
Cerliani, Juan Pablo
Mizrahi, Lina
Pappo, Orit
Rabinovich, Gabriel Adrián
Galun, Eithan
Goldenberg, Daniel S.
author Potikha, Tamara
author_facet Potikha, Tamara
Ella, Ezra
Cerliani, Juan Pablo
Mizrahi, Lina
Pappo, Orit
Rabinovich, Gabriel Adrián
Galun, Eithan
Goldenberg, Daniel S.
author_role author
author2 Ella, Ezra
Cerliani, Juan Pablo
Mizrahi, Lina
Pappo, Orit
Rabinovich, Gabriel Adrián
Galun, Eithan
Goldenberg, Daniel S.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv LIVER REGENERATION
GALECTIN-1
HEPATECTOMY
LIPID METABOLISM
topic LIVER REGENERATION
GALECTIN-1
HEPATECTOMY
LIPID METABOLISM
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Galectin-1 (Gal1) is a known immune/inflammatory regulator which actsboth extracellularly and intracellularly, modulating innate and adaptive immuneresponses. Here, we explored the role of Gal1 in liver regeneration using 70% partial hepatectomy (PHx) of C57BL/6 wild type and Gal1-knockout (Gal1-KO, Lgals1-/-) mice. Gene or protein expression, in liver samples collected at time intervals from 2 to 168 hours post-operation, was tested by either RT-PCR or by immunoblotting and immunohistochemistry, respectively. We demonstrated that Gal1 transcript and protein expression was induced in the liver tissue of wild type mice upon PHx. Liver regeneration following PHx was significantly delayed in the Gal1-KO compared to the control liver. This delay was accompanied by a decreased Akt phosphorylation, and accumulation of the hepatocyte nuclear p21 protein in the Gal1-KO versus control livers at 24 and 48 hours following PHx. Transcripts of several known regulators of inflammation, cell cycle and cell signaling, including some known PHx-induced genes, were aberrantly expressed (mainly down-regulated) in Gal1-KO compared to control livers at 2, 6 and 24 hours post-PHx. Transient steatosis, which is imperative for liver regeneration following PHx, was significantly delayed and decreased in the Gal1- KO compared to the control liver and was accompanied by a significantly decreased expression in the mutant liver of several genes encoding lipid metabolism regulators.Our results demonstrate that Gal1 protein is essential for efficient liver regeneration following PHx through the regulation of liver inflammation, hepatic cell proliferation, and the control of lipid storage in the regenerating liver.
Fil: Potikha, Tamara. Hadassah Hebrew University Medical Center; Israel
Fil: Ella, Ezra. Hadassah Hebrew University Medical Center; Israel
Fil: Cerliani, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Mizrahi, Lina. Hadassah Hebrew University Medical Center; Israel
Fil: Pappo, Orit. Hadassah Hebrew University Medical Center; Israel
Fil: Rabinovich, Gabriel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Galun, Eithan. Hadassah Hebrew University Medical Center; Israel
Fil: Goldenberg, Daniel S.. Hadassah Hebrew University Medical Center; Israel
description Galectin-1 (Gal1) is a known immune/inflammatory regulator which actsboth extracellularly and intracellularly, modulating innate and adaptive immuneresponses. Here, we explored the role of Gal1 in liver regeneration using 70% partial hepatectomy (PHx) of C57BL/6 wild type and Gal1-knockout (Gal1-KO, Lgals1-/-) mice. Gene or protein expression, in liver samples collected at time intervals from 2 to 168 hours post-operation, was tested by either RT-PCR or by immunoblotting and immunohistochemistry, respectively. We demonstrated that Gal1 transcript and protein expression was induced in the liver tissue of wild type mice upon PHx. Liver regeneration following PHx was significantly delayed in the Gal1-KO compared to the control liver. This delay was accompanied by a decreased Akt phosphorylation, and accumulation of the hepatocyte nuclear p21 protein in the Gal1-KO versus control livers at 24 and 48 hours following PHx. Transcripts of several known regulators of inflammation, cell cycle and cell signaling, including some known PHx-induced genes, were aberrantly expressed (mainly down-regulated) in Gal1-KO compared to control livers at 2, 6 and 24 hours post-PHx. Transient steatosis, which is imperative for liver regeneration following PHx, was significantly delayed and decreased in the Gal1- KO compared to the control liver and was accompanied by a significantly decreased expression in the mutant liver of several genes encoding lipid metabolism regulators.Our results demonstrate that Gal1 protein is essential for efficient liver regeneration following PHx through the regulation of liver inflammation, hepatic cell proliferation, and the control of lipid storage in the regenerating liver.
publishDate 2016
dc.date.none.fl_str_mv 2016-05-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/23338
Potikha, Tamara; Ella, Ezra; Cerliani, Juan Pablo; Mizrahi, Lina; Pappo, Orit; et al.; Galectin-1 is essential for efficient liver regeneration following hepatectomy; Impact journals; Oncotarget; 7; 22; 5-5-2016; 31738-31754
1949-2553
1949-2553
CONICET Digital
CONICET
url http://hdl.handle.net/11336/23338
identifier_str_mv Potikha, Tamara; Ella, Ezra; Cerliani, Juan Pablo; Mizrahi, Lina; Pappo, Orit; et al.; Galectin-1 is essential for efficient liver regeneration following hepatectomy; Impact journals; Oncotarget; 7; 22; 5-5-2016; 31738-31754
1949-2553
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.impactjournals.com/oncotarget/index.php?journal=oncotarget&page=article&op=view&path[]=9194&pubmed-linkout=1
info:eu-repo/semantics/altIdentifier/doi/10.18632/oncotarget.9194
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5077973/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Impact journals
publisher.none.fl_str_mv Impact journals
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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