Association between antiarrhythmic, electrophysiological and antioxidative effects of melatonin in ischemia/reperfusion

Autores
Sedova, Ksenia A.; Bernikova, Olesya G.; Cuprova, Julia I.; Ivanova, Alexandra D.; Kutaeva, Galina A.; Pliss, Michael G.; Lopatina, Ekaterina V.; Vaykshnorayte, Marina A.; Diez, Emiliano Raúl; Azarov, Jan E.
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Melatonin is assumed to confer cardioprotective action via antioxidative properties. We evaluated the association between ventricular tachycardia and/or ventricular fibrillation (VT/VF) incidence, oxidative stress, and myocardial electrophysiological parameters in experimental ischemia/reperfusion under melatonin treatment. Melatonin was given to 28 rats (10 mg/kg/day, orally, for 7 days) and 13 animals received placebo. In the anesthetized animals, coronary occlusion was induced for 5 min followed by reperfusion with recording of unipolar electrograms from ventricular epicardium with a 64-lead array. Effects of melatonin on transmembrane potentials were studied in ventricular preparations of 7 rats in normal and ?ischemic? conditions. Melatonin treatment was associated with lower VT/VF incidence at reperfusion, shorter baseline activation times (ATs), and activation-repolarization intervals and more complete recovery of repolarization times (RTs) at reperfusion (less baseline-reperfusion difference, ΔRT) (p < 0.05). Superoxide dismutase (SOD) activity was higher in the treated animals and associated with ΔRT (p = 0.001), whereas VT/VF incidence was associated with baseline ATs (p = 0.020). In vitro, melatonin led to a more complete restoration of action potential durations and resting membrane potentials at reoxygenation (p < 0.05). Thus, the antioxidative properties of melatonin were associated with its influence on repolarization duration, whereas the melatonin-related antiarrhythmic effect was associated with its oxidative stress-independent action on ventricular activation.
Fil: Sedova, Ksenia A.. Czech Technical University In Prague; República Checa
Fil: Bernikova, Olesya G.. Ural Branch of Russian Academy of Sciences; Rusia
Fil: Cuprova, Julia I.. Czech Technical University In Prague; República Checa
Fil: Ivanova, Alexandra D.. Lomonosov Moscow State University; Rusia
Fil: Kutaeva, Galina A.. Pitirim Sorokin Syktyvkar State University; Rusia
Fil: Pliss, Michael G.. Almazov National Medical Research Centre; Rusia
Fil: Lopatina, Ekaterina V.. University of Saint Petersburg; Rusia
Fil: Vaykshnorayte, Marina A.. Ural Branch Of Russian Academy Of Sciences; Rusia
Fil: Diez, Emiliano Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Medicas. Instituto de Fisiologia; Argentina
Fil: Azarov, Jan E.. Ural Branch of Russian Academy of Sciences; Rusia. Pitirim Sorokin Syktyvkar State University; Rusia
Materia
MELATONIN
ISCHEMIA
REPERFUSION
ARRHYTHMIA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/118464
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/118464
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Association between antiarrhythmic, electrophysiological and antioxidative effects of melatonin in ischemia/reperfusionSedova, Ksenia A.Bernikova, Olesya G.Cuprova, Julia I.Ivanova, Alexandra D.Kutaeva, Galina A.Pliss, Michael G.Lopatina, Ekaterina V.Vaykshnorayte, Marina A.Diez, Emiliano RaúlAzarov, Jan E.MELATONINISCHEMIAREPERFUSIONARRHYTHMIAhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Melatonin is assumed to confer cardioprotective action via antioxidative properties. We evaluated the association between ventricular tachycardia and/or ventricular fibrillation (VT/VF) incidence, oxidative stress, and myocardial electrophysiological parameters in experimental ischemia/reperfusion under melatonin treatment. Melatonin was given to 28 rats (10 mg/kg/day, orally, for 7 days) and 13 animals received placebo. In the anesthetized animals, coronary occlusion was induced for 5 min followed by reperfusion with recording of unipolar electrograms from ventricular epicardium with a 64-lead array. Effects of melatonin on transmembrane potentials were studied in ventricular preparations of 7 rats in normal and ?ischemic? conditions. Melatonin treatment was associated with lower VT/VF incidence at reperfusion, shorter baseline activation times (ATs), and activation-repolarization intervals and more complete recovery of repolarization times (RTs) at reperfusion (less baseline-reperfusion difference, ΔRT) (p < 0.05). Superoxide dismutase (SOD) activity was higher in the treated animals and associated with ΔRT (p = 0.001), whereas VT/VF incidence was associated with baseline ATs (p = 0.020). In vitro, melatonin led to a more complete restoration of action potential durations and resting membrane potentials at reoxygenation (p < 0.05). Thus, the antioxidative properties of melatonin were associated with its influence on repolarization duration, whereas the melatonin-related antiarrhythmic effect was associated with its oxidative stress-independent action on ventricular activation.Fil: Sedova, Ksenia A.. Czech Technical University In Prague; República ChecaFil: Bernikova, Olesya G.. Ural Branch of Russian Academy of Sciences; RusiaFil: Cuprova, Julia I.. Czech Technical University In Prague; República ChecaFil: Ivanova, Alexandra D.. Lomonosov Moscow State University; RusiaFil: Kutaeva, Galina A.. Pitirim Sorokin Syktyvkar State University; RusiaFil: Pliss, Michael G.. Almazov National Medical Research Centre; RusiaFil: Lopatina, Ekaterina V.. University of Saint Petersburg; RusiaFil: Vaykshnorayte, Marina A.. Ural Branch Of Russian Academy Of Sciences; RusiaFil: Diez, Emiliano Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Medicas. Instituto de Fisiologia; ArgentinaFil: Azarov, Jan E.. Ural Branch of Russian Academy of Sciences; Rusia. Pitirim Sorokin Syktyvkar State University; RusiaMolecular Diversity Preservation International2019-12-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/118464Sedova, Ksenia A.; Bernikova, Olesya G.; Cuprova, Julia I.; Ivanova, Alexandra D.; Kutaeva, Galina A.; et al.; Association between antiarrhythmic, electrophysiological and antioxidative effects of melatonin in ischemia/reperfusion; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 20; 24; 15-12-2019; 1-151661-65961422-0067CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/20/24/6331info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms20246331info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:43:06Zoai:ri.conicet.gov.ar:11336/118464instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:43:06.564CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Association between antiarrhythmic, electrophysiological and antioxidative effects of melatonin in ischemia/reperfusion
title Association between antiarrhythmic, electrophysiological and antioxidative effects of melatonin in ischemia/reperfusion
spellingShingle Association between antiarrhythmic, electrophysiological and antioxidative effects of melatonin in ischemia/reperfusion
Sedova, Ksenia A.
MELATONIN
ISCHEMIA
REPERFUSION
ARRHYTHMIA
title_short Association between antiarrhythmic, electrophysiological and antioxidative effects of melatonin in ischemia/reperfusion
title_full Association between antiarrhythmic, electrophysiological and antioxidative effects of melatonin in ischemia/reperfusion
title_fullStr Association between antiarrhythmic, electrophysiological and antioxidative effects of melatonin in ischemia/reperfusion
title_full_unstemmed Association between antiarrhythmic, electrophysiological and antioxidative effects of melatonin in ischemia/reperfusion
title_sort Association between antiarrhythmic, electrophysiological and antioxidative effects of melatonin in ischemia/reperfusion
dc.creator.none.fl_str_mv Sedova, Ksenia A.
Bernikova, Olesya G.
Cuprova, Julia I.
Ivanova, Alexandra D.
Kutaeva, Galina A.
Pliss, Michael G.
Lopatina, Ekaterina V.
Vaykshnorayte, Marina A.
Diez, Emiliano Raúl
Azarov, Jan E.
author Sedova, Ksenia A.
author_facet Sedova, Ksenia A.
Bernikova, Olesya G.
Cuprova, Julia I.
Ivanova, Alexandra D.
Kutaeva, Galina A.
Pliss, Michael G.
Lopatina, Ekaterina V.
Vaykshnorayte, Marina A.
Diez, Emiliano Raúl
Azarov, Jan E.
author_role author
author2 Bernikova, Olesya G.
Cuprova, Julia I.
Ivanova, Alexandra D.
Kutaeva, Galina A.
Pliss, Michael G.
Lopatina, Ekaterina V.
Vaykshnorayte, Marina A.
Diez, Emiliano Raúl
Azarov, Jan E.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv MELATONIN
ISCHEMIA
REPERFUSION
ARRHYTHMIA
topic MELATONIN
ISCHEMIA
REPERFUSION
ARRHYTHMIA
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Melatonin is assumed to confer cardioprotective action via antioxidative properties. We evaluated the association between ventricular tachycardia and/or ventricular fibrillation (VT/VF) incidence, oxidative stress, and myocardial electrophysiological parameters in experimental ischemia/reperfusion under melatonin treatment. Melatonin was given to 28 rats (10 mg/kg/day, orally, for 7 days) and 13 animals received placebo. In the anesthetized animals, coronary occlusion was induced for 5 min followed by reperfusion with recording of unipolar electrograms from ventricular epicardium with a 64-lead array. Effects of melatonin on transmembrane potentials were studied in ventricular preparations of 7 rats in normal and ?ischemic? conditions. Melatonin treatment was associated with lower VT/VF incidence at reperfusion, shorter baseline activation times (ATs), and activation-repolarization intervals and more complete recovery of repolarization times (RTs) at reperfusion (less baseline-reperfusion difference, ΔRT) (p < 0.05). Superoxide dismutase (SOD) activity was higher in the treated animals and associated with ΔRT (p = 0.001), whereas VT/VF incidence was associated with baseline ATs (p = 0.020). In vitro, melatonin led to a more complete restoration of action potential durations and resting membrane potentials at reoxygenation (p < 0.05). Thus, the antioxidative properties of melatonin were associated with its influence on repolarization duration, whereas the melatonin-related antiarrhythmic effect was associated with its oxidative stress-independent action on ventricular activation.
Fil: Sedova, Ksenia A.. Czech Technical University In Prague; República Checa
Fil: Bernikova, Olesya G.. Ural Branch of Russian Academy of Sciences; Rusia
Fil: Cuprova, Julia I.. Czech Technical University In Prague; República Checa
Fil: Ivanova, Alexandra D.. Lomonosov Moscow State University; Rusia
Fil: Kutaeva, Galina A.. Pitirim Sorokin Syktyvkar State University; Rusia
Fil: Pliss, Michael G.. Almazov National Medical Research Centre; Rusia
Fil: Lopatina, Ekaterina V.. University of Saint Petersburg; Rusia
Fil: Vaykshnorayte, Marina A.. Ural Branch Of Russian Academy Of Sciences; Rusia
Fil: Diez, Emiliano Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Medicas. Instituto de Fisiologia; Argentina
Fil: Azarov, Jan E.. Ural Branch of Russian Academy of Sciences; Rusia. Pitirim Sorokin Syktyvkar State University; Rusia
description Melatonin is assumed to confer cardioprotective action via antioxidative properties. We evaluated the association between ventricular tachycardia and/or ventricular fibrillation (VT/VF) incidence, oxidative stress, and myocardial electrophysiological parameters in experimental ischemia/reperfusion under melatonin treatment. Melatonin was given to 28 rats (10 mg/kg/day, orally, for 7 days) and 13 animals received placebo. In the anesthetized animals, coronary occlusion was induced for 5 min followed by reperfusion with recording of unipolar electrograms from ventricular epicardium with a 64-lead array. Effects of melatonin on transmembrane potentials were studied in ventricular preparations of 7 rats in normal and ?ischemic? conditions. Melatonin treatment was associated with lower VT/VF incidence at reperfusion, shorter baseline activation times (ATs), and activation-repolarization intervals and more complete recovery of repolarization times (RTs) at reperfusion (less baseline-reperfusion difference, ΔRT) (p < 0.05). Superoxide dismutase (SOD) activity was higher in the treated animals and associated with ΔRT (p = 0.001), whereas VT/VF incidence was associated with baseline ATs (p = 0.020). In vitro, melatonin led to a more complete restoration of action potential durations and resting membrane potentials at reoxygenation (p < 0.05). Thus, the antioxidative properties of melatonin were associated with its influence on repolarization duration, whereas the melatonin-related antiarrhythmic effect was associated with its oxidative stress-independent action on ventricular activation.
publishDate 2019
dc.date.none.fl_str_mv 2019-12-15
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/118464
Sedova, Ksenia A.; Bernikova, Olesya G.; Cuprova, Julia I.; Ivanova, Alexandra D.; Kutaeva, Galina A.; et al.; Association between antiarrhythmic, electrophysiological and antioxidative effects of melatonin in ischemia/reperfusion; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 20; 24; 15-12-2019; 1-15
1661-6596
1422-0067
CONICET Digital
CONICET
url http://hdl.handle.net/11336/118464
identifier_str_mv Sedova, Ksenia A.; Bernikova, Olesya G.; Cuprova, Julia I.; Ivanova, Alexandra D.; Kutaeva, Galina A.; et al.; Association between antiarrhythmic, electrophysiological and antioxidative effects of melatonin in ischemia/reperfusion; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 20; 24; 15-12-2019; 1-15
1661-6596
1422-0067
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/20/24/6331
info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms20246331
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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