A novel electrophysiologic effect of melatonin on ischemia/reperfusion- induced arrhythmias in isolated rat hearts

Autores
Diez, Emiliano Raúl; Prados, Laura Vanina; Carrión, Adriana; Ponce Zumino, Amira Zulma; Miatello, Roberto Miguel
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Reperfusion after a short period of cardiac ischemia triggers ventricular arrhythmias attributable to ionic imbalance and oxidative stress. Melatonin offers some degree of protection, but its effects on the cardiac action potentials are unknown. We evaluated the effects of 5, 10, 20 and 50 μm melatonin in isolated perfused rat hearts subjected to 10 min of regional ischemia. ECG and membrane potentials were synchronously displayed. After 15 min of reperfusion, total antioxidant capacity (TAC) was determined. Melatonin did not change the ischemic depolarization nor the action potential amplitude depression, but at the end of ischemia the action potential duration (APD) decreased in control and 5 μm melatonin-treated hearts. By contrast, it returned to preischemic levels in hearts given 20 and 50 μm melatonin. Melatonin reduced the incidence of reperfusion arrhythmias from 100% in control to 50% in 5 and 10 μm, to 40% in 20 μm and 30% in 50 μm hearts. TAC values were higher at all melatonin concentrations. We conclude that melatonin reduced the incidence of reperfusion arrhythmias because of its antioxidant effects. In addition, at 20 and 50 μm lengthened APD and promoted an improved protection. This latter effect should be considered when in vivo applications of melatonin are considered.
Fil: Diez, Emiliano Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Prados, Laura Vanina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Carrión, Adriana. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Ponce Zumino, Amira Zulma. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Miatello, Roberto Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Materia
Antioxidant
Arrhythmias
Electrophysiology
Ischemia-Reperfusion Injury
Melatonin
Rat
Transmembrane Action Potential
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/79665
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/79665
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A novel electrophysiologic effect of melatonin on ischemia/reperfusion- induced arrhythmias in isolated rat heartsDiez, Emiliano RaúlPrados, Laura VaninaCarrión, AdrianaPonce Zumino, Amira ZulmaMiatello, Roberto MiguelAntioxidantArrhythmiasElectrophysiologyIschemia-Reperfusion InjuryMelatoninRatTransmembrane Action Potentialhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Reperfusion after a short period of cardiac ischemia triggers ventricular arrhythmias attributable to ionic imbalance and oxidative stress. Melatonin offers some degree of protection, but its effects on the cardiac action potentials are unknown. We evaluated the effects of 5, 10, 20 and 50 μm melatonin in isolated perfused rat hearts subjected to 10 min of regional ischemia. ECG and membrane potentials were synchronously displayed. After 15 min of reperfusion, total antioxidant capacity (TAC) was determined. Melatonin did not change the ischemic depolarization nor the action potential amplitude depression, but at the end of ischemia the action potential duration (APD) decreased in control and 5 μm melatonin-treated hearts. By contrast, it returned to preischemic levels in hearts given 20 and 50 μm melatonin. Melatonin reduced the incidence of reperfusion arrhythmias from 100% in control to 50% in 5 and 10 μm, to 40% in 20 μm and 30% in 50 μm hearts. TAC values were higher at all melatonin concentrations. We conclude that melatonin reduced the incidence of reperfusion arrhythmias because of its antioxidant effects. In addition, at 20 and 50 μm lengthened APD and promoted an improved protection. This latter effect should be considered when in vivo applications of melatonin are considered.Fil: Diez, Emiliano Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaFil: Prados, Laura Vanina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaFil: Carrión, Adriana. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaFil: Ponce Zumino, Amira Zulma. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Miatello, Roberto Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaWiley Blackwell Publishing, Inc2009-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/79665Diez, Emiliano Raúl; Prados, Laura Vanina; Carrión, Adriana; Ponce Zumino, Amira Zulma; Miatello, Roberto Miguel; A novel electrophysiologic effect of melatonin on ischemia/reperfusion- induced arrhythmias in isolated rat hearts; Wiley Blackwell Publishing, Inc; Journal of Pineal Research; 46; 2; 3-2009; 155-1600742-3098CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1600-079X.2008.00643.xinfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1600-079X.2008.00643.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:48:45Zoai:ri.conicet.gov.ar:11336/79665instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:48:45.854CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A novel electrophysiologic effect of melatonin on ischemia/reperfusion- induced arrhythmias in isolated rat hearts
title A novel electrophysiologic effect of melatonin on ischemia/reperfusion- induced arrhythmias in isolated rat hearts
spellingShingle A novel electrophysiologic effect of melatonin on ischemia/reperfusion- induced arrhythmias in isolated rat hearts
Diez, Emiliano Raúl
Antioxidant
Arrhythmias
Electrophysiology
Ischemia-Reperfusion Injury
Melatonin
Rat
Transmembrane Action Potential
title_short A novel electrophysiologic effect of melatonin on ischemia/reperfusion- induced arrhythmias in isolated rat hearts
title_full A novel electrophysiologic effect of melatonin on ischemia/reperfusion- induced arrhythmias in isolated rat hearts
title_fullStr A novel electrophysiologic effect of melatonin on ischemia/reperfusion- induced arrhythmias in isolated rat hearts
title_full_unstemmed A novel electrophysiologic effect of melatonin on ischemia/reperfusion- induced arrhythmias in isolated rat hearts
title_sort A novel electrophysiologic effect of melatonin on ischemia/reperfusion- induced arrhythmias in isolated rat hearts
dc.creator.none.fl_str_mv Diez, Emiliano Raúl
Prados, Laura Vanina
Carrión, Adriana
Ponce Zumino, Amira Zulma
Miatello, Roberto Miguel
author Diez, Emiliano Raúl
author_facet Diez, Emiliano Raúl
Prados, Laura Vanina
Carrión, Adriana
Ponce Zumino, Amira Zulma
Miatello, Roberto Miguel
author_role author
author2 Prados, Laura Vanina
Carrión, Adriana
Ponce Zumino, Amira Zulma
Miatello, Roberto Miguel
author2_role author
author
author
author
dc.subject.none.fl_str_mv Antioxidant
Arrhythmias
Electrophysiology
Ischemia-Reperfusion Injury
Melatonin
Rat
Transmembrane Action Potential
topic Antioxidant
Arrhythmias
Electrophysiology
Ischemia-Reperfusion Injury
Melatonin
Rat
Transmembrane Action Potential
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Reperfusion after a short period of cardiac ischemia triggers ventricular arrhythmias attributable to ionic imbalance and oxidative stress. Melatonin offers some degree of protection, but its effects on the cardiac action potentials are unknown. We evaluated the effects of 5, 10, 20 and 50 μm melatonin in isolated perfused rat hearts subjected to 10 min of regional ischemia. ECG and membrane potentials were synchronously displayed. After 15 min of reperfusion, total antioxidant capacity (TAC) was determined. Melatonin did not change the ischemic depolarization nor the action potential amplitude depression, but at the end of ischemia the action potential duration (APD) decreased in control and 5 μm melatonin-treated hearts. By contrast, it returned to preischemic levels in hearts given 20 and 50 μm melatonin. Melatonin reduced the incidence of reperfusion arrhythmias from 100% in control to 50% in 5 and 10 μm, to 40% in 20 μm and 30% in 50 μm hearts. TAC values were higher at all melatonin concentrations. We conclude that melatonin reduced the incidence of reperfusion arrhythmias because of its antioxidant effects. In addition, at 20 and 50 μm lengthened APD and promoted an improved protection. This latter effect should be considered when in vivo applications of melatonin are considered.
Fil: Diez, Emiliano Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Prados, Laura Vanina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Carrión, Adriana. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Ponce Zumino, Amira Zulma. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Miatello, Roberto Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
description Reperfusion after a short period of cardiac ischemia triggers ventricular arrhythmias attributable to ionic imbalance and oxidative stress. Melatonin offers some degree of protection, but its effects on the cardiac action potentials are unknown. We evaluated the effects of 5, 10, 20 and 50 μm melatonin in isolated perfused rat hearts subjected to 10 min of regional ischemia. ECG and membrane potentials were synchronously displayed. After 15 min of reperfusion, total antioxidant capacity (TAC) was determined. Melatonin did not change the ischemic depolarization nor the action potential amplitude depression, but at the end of ischemia the action potential duration (APD) decreased in control and 5 μm melatonin-treated hearts. By contrast, it returned to preischemic levels in hearts given 20 and 50 μm melatonin. Melatonin reduced the incidence of reperfusion arrhythmias from 100% in control to 50% in 5 and 10 μm, to 40% in 20 μm and 30% in 50 μm hearts. TAC values were higher at all melatonin concentrations. We conclude that melatonin reduced the incidence of reperfusion arrhythmias because of its antioxidant effects. In addition, at 20 and 50 μm lengthened APD and promoted an improved protection. This latter effect should be considered when in vivo applications of melatonin are considered.
publishDate 2009
dc.date.none.fl_str_mv 2009-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/79665
Diez, Emiliano Raúl; Prados, Laura Vanina; Carrión, Adriana; Ponce Zumino, Amira Zulma; Miatello, Roberto Miguel; A novel electrophysiologic effect of melatonin on ischemia/reperfusion- induced arrhythmias in isolated rat hearts; Wiley Blackwell Publishing, Inc; Journal of Pineal Research; 46; 2; 3-2009; 155-160
0742-3098
CONICET Digital
CONICET
url http://hdl.handle.net/11336/79665
identifier_str_mv Diez, Emiliano Raúl; Prados, Laura Vanina; Carrión, Adriana; Ponce Zumino, Amira Zulma; Miatello, Roberto Miguel; A novel electrophysiologic effect of melatonin on ischemia/reperfusion- induced arrhythmias in isolated rat hearts; Wiley Blackwell Publishing, Inc; Journal of Pineal Research; 46; 2; 3-2009; 155-160
0742-3098
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1600-079X.2008.00643.x
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1600-079X.2008.00643.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083008814120960
score 13.22299

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