Taxonomic characterization and secondary metabolite profiling of aspergillus section Aspergillus contaminating feeds and feedstuffs

Autores
Greco, Mariana Vanesa; Kemppainen, Minna Johanna; Pose, Graciela Noemi; Pardo, Alejandro Guillermo
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Xerophilic fungal species of the genus Aspergillus are economically highly relevant due to their ability to grow on low water activity substrates causing spoilage of stored goods and animal feeds. These fungi can synthesize a variety of secondary metabolites, many of which show animal toxicity, creating a health risk for food production animals and to humans as final consumers, respectively. Animal feeds used for rabbit, chinchilla and rainbow trout production in Argentina were analysed for the presence of xerophilic Aspergillus section Aspergillus species. High isolation frequencies (>60%) were detected in all the studied rabbit and chinchilla feeds, while the rainbow trout feeds showed lower fungal charge (25%). These section Aspergillus contaminations comprised predominantly five taxa. Twenty isolates were subjected to taxonomic characterization using both ascospore SEM micromorphology and two independent DNA loci sequencing. The secondary metabolite profiles of the isolates were determined qualitatively by HPLC-MS. All the isolates produced neoechinulin A, 17 isolates were positive for cladosporin and echinulin, and 18 were positive for neoechinulin B. Physcion and preechinulin were detected in a minor proportion of the isolates. This is the first report describing the detailed species composition and the secondary metabolite profiles of Aspergillus section Aspergillus contaminating animal feeds.
Fil: Greco, Mariana Vanesa. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Micología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Kemppainen, Minna Johanna. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Micología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pose, Graciela Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rio Negro. Sede Alto Valle. Escuela de Producción, Tecnología y Medio Ambiente; Argentina
Fil: Pardo, Alejandro Guillermo. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Micología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
ANIMAL FEED SPOILAGE
MYCOTOXINS
SCANNING ELECTRON MICROSCOPY
TELEOMORPHS
XEROPHILIC SPECIES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/55050
Acceder
id CONICETDig_a6ecad8496881069e65a08745e167a49
oai_identifier_str oai:ri.conicet.gov.ar:11336/55050
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Taxonomic characterization and secondary metabolite profiling of aspergillus section Aspergillus contaminating feeds and feedstuffsGreco, Mariana VanesaKemppainen, Minna JohannaPose, Graciela NoemiPardo, Alejandro GuillermoANIMAL FEED SPOILAGEMYCOTOXINSSCANNING ELECTRON MICROSCOPYTELEOMORPHSXEROPHILIC SPECIEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Xerophilic fungal species of the genus Aspergillus are economically highly relevant due to their ability to grow on low water activity substrates causing spoilage of stored goods and animal feeds. These fungi can synthesize a variety of secondary metabolites, many of which show animal toxicity, creating a health risk for food production animals and to humans as final consumers, respectively. Animal feeds used for rabbit, chinchilla and rainbow trout production in Argentina were analysed for the presence of xerophilic Aspergillus section Aspergillus species. High isolation frequencies (>60%) were detected in all the studied rabbit and chinchilla feeds, while the rainbow trout feeds showed lower fungal charge (25%). These section Aspergillus contaminations comprised predominantly five taxa. Twenty isolates were subjected to taxonomic characterization using both ascospore SEM micromorphology and two independent DNA loci sequencing. The secondary metabolite profiles of the isolates were determined qualitatively by HPLC-MS. All the isolates produced neoechinulin A, 17 isolates were positive for cladosporin and echinulin, and 18 were positive for neoechinulin B. Physcion and preechinulin were detected in a minor proportion of the isolates. This is the first report describing the detailed species composition and the secondary metabolite profiles of Aspergillus section Aspergillus contaminating animal feeds.Fil: Greco, Mariana Vanesa. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Micología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Kemppainen, Minna Johanna. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Micología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pose, Graciela Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rio Negro. Sede Alto Valle. Escuela de Producción, Tecnología y Medio Ambiente; ArgentinaFil: Pardo, Alejandro Guillermo. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Micología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaMDPI2015-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/55050Greco, Mariana Vanesa; Kemppainen, Minna Johanna; Pose, Graciela Noemi; Pardo, Alejandro Guillermo; Taxonomic characterization and secondary metabolite profiling of aspergillus section Aspergillus contaminating feeds and feedstuffs; MDPI; Toxins; 7; 9; 9-2015; 3512-35372072-6651CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/toxins7093512info:eu-repo/semantics/altIdentifier/url/http://www.mdpi.com/2072-6651/7/9/3512info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:32:15Zoai:ri.conicet.gov.ar:11336/55050instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:32:16.048CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Taxonomic characterization and secondary metabolite profiling of aspergillus section Aspergillus contaminating feeds and feedstuffs
title Taxonomic characterization and secondary metabolite profiling of aspergillus section Aspergillus contaminating feeds and feedstuffs
spellingShingle Taxonomic characterization and secondary metabolite profiling of aspergillus section Aspergillus contaminating feeds and feedstuffs
Greco, Mariana Vanesa
ANIMAL FEED SPOILAGE
MYCOTOXINS
SCANNING ELECTRON MICROSCOPY
TELEOMORPHS
XEROPHILIC SPECIES
title_short Taxonomic characterization and secondary metabolite profiling of aspergillus section Aspergillus contaminating feeds and feedstuffs
title_full Taxonomic characterization and secondary metabolite profiling of aspergillus section Aspergillus contaminating feeds and feedstuffs
title_fullStr Taxonomic characterization and secondary metabolite profiling of aspergillus section Aspergillus contaminating feeds and feedstuffs
title_full_unstemmed Taxonomic characterization and secondary metabolite profiling of aspergillus section Aspergillus contaminating feeds and feedstuffs
title_sort Taxonomic characterization and secondary metabolite profiling of aspergillus section Aspergillus contaminating feeds and feedstuffs
dc.creator.none.fl_str_mv Greco, Mariana Vanesa
Kemppainen, Minna Johanna
Pose, Graciela Noemi
Pardo, Alejandro Guillermo
author Greco, Mariana Vanesa
author_facet Greco, Mariana Vanesa
Kemppainen, Minna Johanna
Pose, Graciela Noemi
Pardo, Alejandro Guillermo
author_role author
author2 Kemppainen, Minna Johanna
Pose, Graciela Noemi
Pardo, Alejandro Guillermo
author2_role author
author
author
dc.subject.none.fl_str_mv ANIMAL FEED SPOILAGE
MYCOTOXINS
SCANNING ELECTRON MICROSCOPY
TELEOMORPHS
XEROPHILIC SPECIES
topic ANIMAL FEED SPOILAGE
MYCOTOXINS
SCANNING ELECTRON MICROSCOPY
TELEOMORPHS
XEROPHILIC SPECIES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Xerophilic fungal species of the genus Aspergillus are economically highly relevant due to their ability to grow on low water activity substrates causing spoilage of stored goods and animal feeds. These fungi can synthesize a variety of secondary metabolites, many of which show animal toxicity, creating a health risk for food production animals and to humans as final consumers, respectively. Animal feeds used for rabbit, chinchilla and rainbow trout production in Argentina were analysed for the presence of xerophilic Aspergillus section Aspergillus species. High isolation frequencies (>60%) were detected in all the studied rabbit and chinchilla feeds, while the rainbow trout feeds showed lower fungal charge (25%). These section Aspergillus contaminations comprised predominantly five taxa. Twenty isolates were subjected to taxonomic characterization using both ascospore SEM micromorphology and two independent DNA loci sequencing. The secondary metabolite profiles of the isolates were determined qualitatively by HPLC-MS. All the isolates produced neoechinulin A, 17 isolates were positive for cladosporin and echinulin, and 18 were positive for neoechinulin B. Physcion and preechinulin were detected in a minor proportion of the isolates. This is the first report describing the detailed species composition and the secondary metabolite profiles of Aspergillus section Aspergillus contaminating animal feeds.
Fil: Greco, Mariana Vanesa. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Micología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Kemppainen, Minna Johanna. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Micología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pose, Graciela Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rio Negro. Sede Alto Valle. Escuela de Producción, Tecnología y Medio Ambiente; Argentina
Fil: Pardo, Alejandro Guillermo. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Micología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Xerophilic fungal species of the genus Aspergillus are economically highly relevant due to their ability to grow on low water activity substrates causing spoilage of stored goods and animal feeds. These fungi can synthesize a variety of secondary metabolites, many of which show animal toxicity, creating a health risk for food production animals and to humans as final consumers, respectively. Animal feeds used for rabbit, chinchilla and rainbow trout production in Argentina were analysed for the presence of xerophilic Aspergillus section Aspergillus species. High isolation frequencies (>60%) were detected in all the studied rabbit and chinchilla feeds, while the rainbow trout feeds showed lower fungal charge (25%). These section Aspergillus contaminations comprised predominantly five taxa. Twenty isolates were subjected to taxonomic characterization using both ascospore SEM micromorphology and two independent DNA loci sequencing. The secondary metabolite profiles of the isolates were determined qualitatively by HPLC-MS. All the isolates produced neoechinulin A, 17 isolates were positive for cladosporin and echinulin, and 18 were positive for neoechinulin B. Physcion and preechinulin were detected in a minor proportion of the isolates. This is the first report describing the detailed species composition and the secondary metabolite profiles of Aspergillus section Aspergillus contaminating animal feeds.
publishDate 2015
dc.date.none.fl_str_mv 2015-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/55050
Greco, Mariana Vanesa; Kemppainen, Minna Johanna; Pose, Graciela Noemi; Pardo, Alejandro Guillermo; Taxonomic characterization and secondary metabolite profiling of aspergillus section Aspergillus contaminating feeds and feedstuffs; MDPI; Toxins; 7; 9; 9-2015; 3512-3537
2072-6651
CONICET Digital
CONICET
url http://hdl.handle.net/11336/55050
identifier_str_mv Greco, Mariana Vanesa; Kemppainen, Minna Johanna; Pose, Graciela Noemi; Pardo, Alejandro Guillermo; Taxonomic characterization and secondary metabolite profiling of aspergillus section Aspergillus contaminating feeds and feedstuffs; MDPI; Toxins; 7; 9; 9-2015; 3512-3537
2072-6651
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/toxins7093512
info:eu-repo/semantics/altIdentifier/url/http://www.mdpi.com/2072-6651/7/9/3512
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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