Solid phase microextraction chemical biopsy tool for monitoring of doxorubicin residue during in vivo lung chemo-perfusion

Autores
Bojko, Barbara; Looby, Nikita; Olkowicz, Mariola; Roszkowska, Anna; Kupcewicz, Bogumiła; Reck des Santos, Pedro; Ramadan, Khaled; Keshavjee, Shaf; Waddell, Thomas K.; Gómez Ríos, German; Tascon, Marcos; Goryński, Krzysztof; Cypel, Marcelo; Pawliszyn, Janusz
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Development of a novel in vivo lung perfusion (IVLP) procedure allows localized delivery of high-dose doxorubicin (DOX) for targeting residual micrometastatic disease in the lungs. However, DOX delivery via IVLP requires careful monitoring of drug level to ensure tissue concentrations of this agent remain in the therapeutic window. A small dimension nitinol wire coated with a sorbent of biocompatible morphology (Bio-SPME) has been clinically evaluated for in vivo lung tissue extraction and determination of DOX and its key metabolites. The in vivo Bio-SPME-IVLP experiments were performed on pig model over various (150 and 225 mg/m2) drug doses, and during human clinical trial. Two patients with metastatic osteosarcoma were treated with a single 5 and 7 μg/mL (respectively) dose of DOX during a 3-h IVLP. In both pig and human cases, DOX tissue levels presented similar trends during IVLP. Human lung tissue concentrations of drug ranged between 15 and 293 μg/g over the course of the IVLP procedure. In addition to DOX levels, Bio-SPME followed by liquid chromatography-mass spectrometry analysis generated 64 metabolic features during endogenous metabolite screening, providing information about lung status during drug administration. Real-time monitoring of DOX levels in the lungs can be performed effectively throughout the IVLP procedure by in vivo Bio-SPME chemical biopsy approach. Bio-SPME also extracted various endogenous molecules, thus providing a real-time snapshot of the physiology of the cells, which might assist in the tailoring of personalized treatment strategy.
Fil: Bojko, Barbara. University of Waterloo; Canadá
Fil: Looby, Nikita. University of Waterloo; Canadá
Fil: Olkowicz, Mariola. University of Waterloo; Canadá
Fil: Roszkowska, Anna. University of Waterloo; Canadá
Fil: Kupcewicz, Bogumiła. Nicolaus Copernicus University in Torun; Polonia
Fil: Reck des Santos, Pedro. University Health Network; Canadá
Fil: Ramadan, Khaled. University Health Network; Canadá
Fil: Keshavjee, Shaf. University Health Network; Canadá
Fil: Waddell, Thomas K.. University Health Network; Canadá
Fil: Gómez Ríos, German. University of Waterloo; Canadá
Fil: Tascon, Marcos. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación e Ingeniería Ambiental; Argentina
Fil: Goryński, Krzysztof. University of Waterloo; Canadá
Fil: Cypel, Marcelo. University Health Network; Canadá
Fil: Pawliszyn, Janusz. University of Waterloo; Canadá
Materia
IN VIVO SOLID PHASE MICROEXTRACTION
METABOLITE PROFILING
SPATIAL RESOLUTION
THERAPEUTIC DRUG MONITORING
TISSUE ANALYSIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/142888
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/142888
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Solid phase microextraction chemical biopsy tool for monitoring of doxorubicin residue during in vivo lung chemo-perfusionBojko, BarbaraLooby, NikitaOlkowicz, MariolaRoszkowska, AnnaKupcewicz, BogumiłaReck des Santos, PedroRamadan, KhaledKeshavjee, ShafWaddell, Thomas K.Gómez Ríos, GermanTascon, MarcosGoryński, KrzysztofCypel, MarceloPawliszyn, JanuszIN VIVO SOLID PHASE MICROEXTRACTIONMETABOLITE PROFILINGSPATIAL RESOLUTIONTHERAPEUTIC DRUG MONITORINGTISSUE ANALYSIShttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Development of a novel in vivo lung perfusion (IVLP) procedure allows localized delivery of high-dose doxorubicin (DOX) for targeting residual micrometastatic disease in the lungs. However, DOX delivery via IVLP requires careful monitoring of drug level to ensure tissue concentrations of this agent remain in the therapeutic window. A small dimension nitinol wire coated with a sorbent of biocompatible morphology (Bio-SPME) has been clinically evaluated for in vivo lung tissue extraction and determination of DOX and its key metabolites. The in vivo Bio-SPME-IVLP experiments were performed on pig model over various (150 and 225 mg/m2) drug doses, and during human clinical trial. Two patients with metastatic osteosarcoma were treated with a single 5 and 7 μg/mL (respectively) dose of DOX during a 3-h IVLP. In both pig and human cases, DOX tissue levels presented similar trends during IVLP. Human lung tissue concentrations of drug ranged between 15 and 293 μg/g over the course of the IVLP procedure. In addition to DOX levels, Bio-SPME followed by liquid chromatography-mass spectrometry analysis generated 64 metabolic features during endogenous metabolite screening, providing information about lung status during drug administration. Real-time monitoring of DOX levels in the lungs can be performed effectively throughout the IVLP procedure by in vivo Bio-SPME chemical biopsy approach. Bio-SPME also extracted various endogenous molecules, thus providing a real-time snapshot of the physiology of the cells, which might assist in the tailoring of personalized treatment strategy.Fil: Bojko, Barbara. University of Waterloo; CanadáFil: Looby, Nikita. University of Waterloo; CanadáFil: Olkowicz, Mariola. University of Waterloo; CanadáFil: Roszkowska, Anna. University of Waterloo; CanadáFil: Kupcewicz, Bogumiła. Nicolaus Copernicus University in Torun; PoloniaFil: Reck des Santos, Pedro. University Health Network; CanadáFil: Ramadan, Khaled. University Health Network; CanadáFil: Keshavjee, Shaf. University Health Network; CanadáFil: Waddell, Thomas K.. University Health Network; CanadáFil: Gómez Ríos, German. University of Waterloo; CanadáFil: Tascon, Marcos. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación e Ingeniería Ambiental; ArgentinaFil: Goryński, Krzysztof. University of Waterloo; CanadáFil: Cypel, Marcelo. University Health Network; CanadáFil: Pawliszyn, Janusz. University of Waterloo; CanadáElsevier2021-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/142888Bojko, Barbara; Looby, Nikita; Olkowicz, Mariola; Roszkowska, Anna; Kupcewicz, Bogumiła; et al.; Solid phase microextraction chemical biopsy tool for monitoring of doxorubicin residue during in vivo lung chemo-perfusion; Elsevier; Journal of Pharmaceutical Analysis; 11; 1; 2-2021; 37-472095-1779CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2095177920310546info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jpha.2020.08.011info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:25:33Zoai:ri.conicet.gov.ar:11336/142888instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:25:34.098CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Solid phase microextraction chemical biopsy tool for monitoring of doxorubicin residue during in vivo lung chemo-perfusion
title Solid phase microextraction chemical biopsy tool for monitoring of doxorubicin residue during in vivo lung chemo-perfusion
spellingShingle Solid phase microextraction chemical biopsy tool for monitoring of doxorubicin residue during in vivo lung chemo-perfusion
Bojko, Barbara
IN VIVO SOLID PHASE MICROEXTRACTION
METABOLITE PROFILING
SPATIAL RESOLUTION
THERAPEUTIC DRUG MONITORING
TISSUE ANALYSIS
title_short Solid phase microextraction chemical biopsy tool for monitoring of doxorubicin residue during in vivo lung chemo-perfusion
title_full Solid phase microextraction chemical biopsy tool for monitoring of doxorubicin residue during in vivo lung chemo-perfusion
title_fullStr Solid phase microextraction chemical biopsy tool for monitoring of doxorubicin residue during in vivo lung chemo-perfusion
title_full_unstemmed Solid phase microextraction chemical biopsy tool for monitoring of doxorubicin residue during in vivo lung chemo-perfusion
title_sort Solid phase microextraction chemical biopsy tool for monitoring of doxorubicin residue during in vivo lung chemo-perfusion
dc.creator.none.fl_str_mv Bojko, Barbara
Looby, Nikita
Olkowicz, Mariola
Roszkowska, Anna
Kupcewicz, Bogumiła
Reck des Santos, Pedro
Ramadan, Khaled
Keshavjee, Shaf
Waddell, Thomas K.
Gómez Ríos, German
Tascon, Marcos
Goryński, Krzysztof
Cypel, Marcelo
Pawliszyn, Janusz
author Bojko, Barbara
author_facet Bojko, Barbara
Looby, Nikita
Olkowicz, Mariola
Roszkowska, Anna
Kupcewicz, Bogumiła
Reck des Santos, Pedro
Ramadan, Khaled
Keshavjee, Shaf
Waddell, Thomas K.
Gómez Ríos, German
Tascon, Marcos
Goryński, Krzysztof
Cypel, Marcelo
Pawliszyn, Janusz
author_role author
author2 Looby, Nikita
Olkowicz, Mariola
Roszkowska, Anna
Kupcewicz, Bogumiła
Reck des Santos, Pedro
Ramadan, Khaled
Keshavjee, Shaf
Waddell, Thomas K.
Gómez Ríos, German
Tascon, Marcos
Goryński, Krzysztof
Cypel, Marcelo
Pawliszyn, Janusz
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv IN VIVO SOLID PHASE MICROEXTRACTION
METABOLITE PROFILING
SPATIAL RESOLUTION
THERAPEUTIC DRUG MONITORING
TISSUE ANALYSIS
topic IN VIVO SOLID PHASE MICROEXTRACTION
METABOLITE PROFILING
SPATIAL RESOLUTION
THERAPEUTIC DRUG MONITORING
TISSUE ANALYSIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Development of a novel in vivo lung perfusion (IVLP) procedure allows localized delivery of high-dose doxorubicin (DOX) for targeting residual micrometastatic disease in the lungs. However, DOX delivery via IVLP requires careful monitoring of drug level to ensure tissue concentrations of this agent remain in the therapeutic window. A small dimension nitinol wire coated with a sorbent of biocompatible morphology (Bio-SPME) has been clinically evaluated for in vivo lung tissue extraction and determination of DOX and its key metabolites. The in vivo Bio-SPME-IVLP experiments were performed on pig model over various (150 and 225 mg/m2) drug doses, and during human clinical trial. Two patients with metastatic osteosarcoma were treated with a single 5 and 7 μg/mL (respectively) dose of DOX during a 3-h IVLP. In both pig and human cases, DOX tissue levels presented similar trends during IVLP. Human lung tissue concentrations of drug ranged between 15 and 293 μg/g over the course of the IVLP procedure. In addition to DOX levels, Bio-SPME followed by liquid chromatography-mass spectrometry analysis generated 64 metabolic features during endogenous metabolite screening, providing information about lung status during drug administration. Real-time monitoring of DOX levels in the lungs can be performed effectively throughout the IVLP procedure by in vivo Bio-SPME chemical biopsy approach. Bio-SPME also extracted various endogenous molecules, thus providing a real-time snapshot of the physiology of the cells, which might assist in the tailoring of personalized treatment strategy.
Fil: Bojko, Barbara. University of Waterloo; Canadá
Fil: Looby, Nikita. University of Waterloo; Canadá
Fil: Olkowicz, Mariola. University of Waterloo; Canadá
Fil: Roszkowska, Anna. University of Waterloo; Canadá
Fil: Kupcewicz, Bogumiła. Nicolaus Copernicus University in Torun; Polonia
Fil: Reck des Santos, Pedro. University Health Network; Canadá
Fil: Ramadan, Khaled. University Health Network; Canadá
Fil: Keshavjee, Shaf. University Health Network; Canadá
Fil: Waddell, Thomas K.. University Health Network; Canadá
Fil: Gómez Ríos, German. University of Waterloo; Canadá
Fil: Tascon, Marcos. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación e Ingeniería Ambiental; Argentina
Fil: Goryński, Krzysztof. University of Waterloo; Canadá
Fil: Cypel, Marcelo. University Health Network; Canadá
Fil: Pawliszyn, Janusz. University of Waterloo; Canadá
description Development of a novel in vivo lung perfusion (IVLP) procedure allows localized delivery of high-dose doxorubicin (DOX) for targeting residual micrometastatic disease in the lungs. However, DOX delivery via IVLP requires careful monitoring of drug level to ensure tissue concentrations of this agent remain in the therapeutic window. A small dimension nitinol wire coated with a sorbent of biocompatible morphology (Bio-SPME) has been clinically evaluated for in vivo lung tissue extraction and determination of DOX and its key metabolites. The in vivo Bio-SPME-IVLP experiments were performed on pig model over various (150 and 225 mg/m2) drug doses, and during human clinical trial. Two patients with metastatic osteosarcoma were treated with a single 5 and 7 μg/mL (respectively) dose of DOX during a 3-h IVLP. In both pig and human cases, DOX tissue levels presented similar trends during IVLP. Human lung tissue concentrations of drug ranged between 15 and 293 μg/g over the course of the IVLP procedure. In addition to DOX levels, Bio-SPME followed by liquid chromatography-mass spectrometry analysis generated 64 metabolic features during endogenous metabolite screening, providing information about lung status during drug administration. Real-time monitoring of DOX levels in the lungs can be performed effectively throughout the IVLP procedure by in vivo Bio-SPME chemical biopsy approach. Bio-SPME also extracted various endogenous molecules, thus providing a real-time snapshot of the physiology of the cells, which might assist in the tailoring of personalized treatment strategy.
publishDate 2021
dc.date.none.fl_str_mv 2021-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/142888
Bojko, Barbara; Looby, Nikita; Olkowicz, Mariola; Roszkowska, Anna; Kupcewicz, Bogumiła; et al.; Solid phase microextraction chemical biopsy tool for monitoring of doxorubicin residue during in vivo lung chemo-perfusion; Elsevier; Journal of Pharmaceutical Analysis; 11; 1; 2-2021; 37-47
2095-1779
CONICET Digital
CONICET
url http://hdl.handle.net/11336/142888
identifier_str_mv Bojko, Barbara; Looby, Nikita; Olkowicz, Mariola; Roszkowska, Anna; Kupcewicz, Bogumiła; et al.; Solid phase microextraction chemical biopsy tool for monitoring of doxorubicin residue during in vivo lung chemo-perfusion; Elsevier; Journal of Pharmaceutical Analysis; 11; 1; 2-2021; 37-47
2095-1779
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2095177920310546
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jpha.2020.08.011
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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