Androgen receptors in human melanoma cell lines IIB-MEL-LES and IIB-MEL-IAN and in human melanoma metastases

Autores
Morvillo, V.; Luthy, Isabel Alicia; Bravo, A. I.; Capurro, M. I.; Portela, P.; Calandra, Ricardo Saul; Mordoh, Jose
Año de publicación
2002
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The presence and characteristics of androgen receptors (AR) have been des­cribed by our group in one human melanoma cell line. We now investigated their presence in two other human melanoma cell lines, IIB-MEL-LES and IIB-MEL-IAN, as well as in biopsies of human metastatic melanoma. Scatchard analysis revealed a single binding component for both cell lines, the apparent dissociation constant obtained being 15 nM, with a binding capacity of 280 fmol/mg total cell protein for IIB-MEL-LES cells and of 14 nM with a binding capacity of 206 fmol/mg total cell protein for IIB-MEL-IAN. When specifi­ci­ty was assessed, as seen before, not only androgen and anti­an­dro­gen, but also non-andro­ge­nic com­pounds were able to compete for [3H]-R1881 binding. When immu­nocyto­che­mis­try of IIB-MEL-LES and IIB-MEL-IAN cells was performed for AR, both cell lines were deeply stained in the nu­cleus whe­reas no staining was found for oestrogen and pro­ges­te­rone receptors. Every specimen of mela­no­ma metas­ta­ses tested for the presence of AR was deeply stained, and the intensity of the stai­ning was high in the majority. Several hormones and antihormones were tested for their ability to affect cell proli­fe­ration. On both cell lines, Testosterone, Dihydrotesterone, Oestradiol and Pro­ges­terone significantly stimulated cell proliferation, with reversion by Hydroxy-Flutamide, Casodex or Tamo­xi­fen.
Fil: Morvillo, V.. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Luthy, Isabel Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Bravo, A. I.. Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal de Agudos ; Argentina
Fil: Capurro, M. I.. Instituto Luis Federico Leloir;
Fil: Portela, P.. Instituto Luis Federico Leloir;
Fil: Calandra, Ricardo Saul. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Mordoh, Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Materia
Androgen
Androgen Receptor
Anti-Androgen
Anti-Estrogen
Flutamide
Melanoma
Tamoxifen
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/30847
Acceder
id CONICETDig_a68d935387f4c8d436d270a1e136e769
oai_identifier_str oai:ri.conicet.gov.ar:11336/30847
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Androgen receptors in human melanoma cell lines IIB-MEL-LES and IIB-MEL-IAN and in human melanoma metastasesMorvillo, V.Luthy, Isabel AliciaBravo, A. I.Capurro, M. I.Portela, P.Calandra, Ricardo SaulMordoh, JoseAndrogenAndrogen ReceptorAnti-AndrogenAnti-EstrogenFlutamideMelanomaTamoxifenhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The presence and characteristics of androgen receptors (AR) have been des­cribed by our group in one human melanoma cell line. We now investigated their presence in two other human melanoma cell lines, IIB-MEL-LES and IIB-MEL-IAN, as well as in biopsies of human metastatic melanoma. Scatchard analysis revealed a single binding component for both cell lines, the apparent dissociation constant obtained being 15 nM, with a binding capacity of 280 fmol/mg total cell protein for IIB-MEL-LES cells and of 14 nM with a binding capacity of 206 fmol/mg total cell protein for IIB-MEL-IAN. When specifi­ci­ty was assessed, as seen before, not only androgen and anti­an­dro­gen, but also non-andro­ge­nic com­pounds were able to compete for [3H]-R1881 binding. When immu­nocyto­che­mis­try of IIB-MEL-LES and IIB-MEL-IAN cells was performed for AR, both cell lines were deeply stained in the nu­cleus whe­reas no staining was found for oestrogen and pro­ges­te­rone receptors. Every specimen of mela­no­ma metas­ta­ses tested for the presence of AR was deeply stained, and the intensity of the stai­ning was high in the majority. Several hormones and antihormones were tested for their ability to affect cell proli­fe­ration. On both cell lines, Testosterone, Dihydrotesterone, Oestradiol and Pro­ges­terone significantly stimulated cell proliferation, with reversion by Hydroxy-Flutamide, Casodex or Tamo­xi­fen.Fil: Morvillo, V.. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Luthy, Isabel Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Bravo, A. I.. Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal de Agudos ; ArgentinaFil: Capurro, M. I.. Instituto Luis Federico Leloir;Fil: Portela, P.. Instituto Luis Federico Leloir;Fil: Calandra, Ricardo Saul. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; ArgentinaFil: Mordoh, Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaLippincott Williams2002-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/30847Morvillo, V.; Mordoh, Jose; Calandra, Ricardo Saul; Portela, P.; Capurro, M. I.; Bravo, A. I.; et al.; Androgen receptors in human melanoma cell lines IIB-MEL-LES and IIB-MEL-IAN and in human melanoma metastases; Lippincott Williams; Melanoma Research; 12; 6; 12-2002; 529-5380960-89311473-5636CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://insights.ovid.com/pubmed?pmid=12459642info:eu-repo/semantics/altIdentifier/doi/10.1097/01.cmr.0000043153.28051.51info:eu-repo/semantics/altIdentifier/pmid/12459642info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:33Zoai:ri.conicet.gov.ar:11336/30847instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:34.11CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Androgen receptors in human melanoma cell lines IIB-MEL-LES and IIB-MEL-IAN and in human melanoma metastases
title Androgen receptors in human melanoma cell lines IIB-MEL-LES and IIB-MEL-IAN and in human melanoma metastases
spellingShingle Androgen receptors in human melanoma cell lines IIB-MEL-LES and IIB-MEL-IAN and in human melanoma metastases
Morvillo, V.
Androgen
Androgen Receptor
Anti-Androgen
Anti-Estrogen
Flutamide
Melanoma
Tamoxifen
title_short Androgen receptors in human melanoma cell lines IIB-MEL-LES and IIB-MEL-IAN and in human melanoma metastases
title_full Androgen receptors in human melanoma cell lines IIB-MEL-LES and IIB-MEL-IAN and in human melanoma metastases
title_fullStr Androgen receptors in human melanoma cell lines IIB-MEL-LES and IIB-MEL-IAN and in human melanoma metastases
title_full_unstemmed Androgen receptors in human melanoma cell lines IIB-MEL-LES and IIB-MEL-IAN and in human melanoma metastases
title_sort Androgen receptors in human melanoma cell lines IIB-MEL-LES and IIB-MEL-IAN and in human melanoma metastases
dc.creator.none.fl_str_mv Morvillo, V.
Luthy, Isabel Alicia
Bravo, A. I.
Capurro, M. I.
Portela, P.
Calandra, Ricardo Saul
Mordoh, Jose
author Morvillo, V.
author_facet Morvillo, V.
Luthy, Isabel Alicia
Bravo, A. I.
Capurro, M. I.
Portela, P.
Calandra, Ricardo Saul
Mordoh, Jose
author_role author
author2 Luthy, Isabel Alicia
Bravo, A. I.
Capurro, M. I.
Portela, P.
Calandra, Ricardo Saul
Mordoh, Jose
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Androgen
Androgen Receptor
Anti-Androgen
Anti-Estrogen
Flutamide
Melanoma
Tamoxifen
topic Androgen
Androgen Receptor
Anti-Androgen
Anti-Estrogen
Flutamide
Melanoma
Tamoxifen
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The presence and characteristics of androgen receptors (AR) have been des­cribed by our group in one human melanoma cell line. We now investigated their presence in two other human melanoma cell lines, IIB-MEL-LES and IIB-MEL-IAN, as well as in biopsies of human metastatic melanoma. Scatchard analysis revealed a single binding component for both cell lines, the apparent dissociation constant obtained being 15 nM, with a binding capacity of 280 fmol/mg total cell protein for IIB-MEL-LES cells and of 14 nM with a binding capacity of 206 fmol/mg total cell protein for IIB-MEL-IAN. When specifi­ci­ty was assessed, as seen before, not only androgen and anti­an­dro­gen, but also non-andro­ge­nic com­pounds were able to compete for [3H]-R1881 binding. When immu­nocyto­che­mis­try of IIB-MEL-LES and IIB-MEL-IAN cells was performed for AR, both cell lines were deeply stained in the nu­cleus whe­reas no staining was found for oestrogen and pro­ges­te­rone receptors. Every specimen of mela­no­ma metas­ta­ses tested for the presence of AR was deeply stained, and the intensity of the stai­ning was high in the majority. Several hormones and antihormones were tested for their ability to affect cell proli­fe­ration. On both cell lines, Testosterone, Dihydrotesterone, Oestradiol and Pro­ges­terone significantly stimulated cell proliferation, with reversion by Hydroxy-Flutamide, Casodex or Tamo­xi­fen.
Fil: Morvillo, V.. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Luthy, Isabel Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Bravo, A. I.. Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal de Agudos ; Argentina
Fil: Capurro, M. I.. Instituto Luis Federico Leloir;
Fil: Portela, P.. Instituto Luis Federico Leloir;
Fil: Calandra, Ricardo Saul. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Mordoh, Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
description The presence and characteristics of androgen receptors (AR) have been des­cribed by our group in one human melanoma cell line. We now investigated their presence in two other human melanoma cell lines, IIB-MEL-LES and IIB-MEL-IAN, as well as in biopsies of human metastatic melanoma. Scatchard analysis revealed a single binding component for both cell lines, the apparent dissociation constant obtained being 15 nM, with a binding capacity of 280 fmol/mg total cell protein for IIB-MEL-LES cells and of 14 nM with a binding capacity of 206 fmol/mg total cell protein for IIB-MEL-IAN. When specifi­ci­ty was assessed, as seen before, not only androgen and anti­an­dro­gen, but also non-andro­ge­nic com­pounds were able to compete for [3H]-R1881 binding. When immu­nocyto­che­mis­try of IIB-MEL-LES and IIB-MEL-IAN cells was performed for AR, both cell lines were deeply stained in the nu­cleus whe­reas no staining was found for oestrogen and pro­ges­te­rone receptors. Every specimen of mela­no­ma metas­ta­ses tested for the presence of AR was deeply stained, and the intensity of the stai­ning was high in the majority. Several hormones and antihormones were tested for their ability to affect cell proli­fe­ration. On both cell lines, Testosterone, Dihydrotesterone, Oestradiol and Pro­ges­terone significantly stimulated cell proliferation, with reversion by Hydroxy-Flutamide, Casodex or Tamo­xi­fen.
publishDate 2002
dc.date.none.fl_str_mv 2002-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/30847
Morvillo, V.; Mordoh, Jose; Calandra, Ricardo Saul; Portela, P.; Capurro, M. I.; Bravo, A. I.; et al.; Androgen receptors in human melanoma cell lines IIB-MEL-LES and IIB-MEL-IAN and in human melanoma metastases; Lippincott Williams; Melanoma Research; 12; 6; 12-2002; 529-538
0960-8931
1473-5636
CONICET Digital
CONICET
url http://hdl.handle.net/11336/30847
identifier_str_mv Morvillo, V.; Mordoh, Jose; Calandra, Ricardo Saul; Portela, P.; Capurro, M. I.; Bravo, A. I.; et al.; Androgen receptors in human melanoma cell lines IIB-MEL-LES and IIB-MEL-IAN and in human melanoma metastases; Lippincott Williams; Melanoma Research; 12; 6; 12-2002; 529-538
0960-8931
1473-5636
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://insights.ovid.com/pubmed?pmid=12459642
info:eu-repo/semantics/altIdentifier/doi/10.1097/01.cmr.0000043153.28051.51
info:eu-repo/semantics/altIdentifier/pmid/12459642
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Lippincott Williams
publisher.none.fl_str_mv Lippincott Williams
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268981062270976
score 13.13397

Publicaciones similares