Extended-spectrum β-lactamases, transferable quinolone resistance, and virulotyping in extra-intestinal E. coli in Uruguay

Autores
Vignoli, Rafael; García Fulgueiras, Virginia; Cordeiro, Nicolás F.; Bado, Inés; Seija, Verónica; Aguerrebere, Paula; Laguna, Gabriel; Araújo, Lucía; Bazet, Cristina; Gutkind, Gabriel Osvaldo; Chabalgoity Rodríguez, José Alejandro
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Introduction: To characterize extended-spectrum β-lactamases (ESBLs) and plasmid-mediated quinolone resistance (PMQR) genes in Escherichia coli isolates obtained from extra-intestinal samples in three Uruguayan hospitals. Methodology: Fifty-five ESBL-producing E. coli isolates were studied. Virulence genes, ESBLs, and PMQR genes were detected by polymerase chain reaction. ESBL-producing isolates were compared by pulsed-field gel electrophoresis. Multi-locus sequence typing was also performed on 13 selected isolates. Results: Thirty-seven isolates harbored blaCTX-M-15 (67.3%), eight blaCTX-M-2 (14.6%), five blaCTX-M-14 (9.1%), three carried both blaCTX-M-2 and blaCTX-M-14, one blaCTX-M-9, and one blaCTX-M-8. Among the CTX-M-15 producers, 92% belonged to sequence types ST131 and ST405, and carried aac(6’)Ib-cr as well. Isolates harboring blaCTX-M-2, blaCTX-M-14, blaCTX-M-9, or blaCTX-M-8 were found to be genetically unrelated. Conclusions: The successful dissemination of CTX-M-15-producing E.coli isolates seems to be linked to the spreading of high-risk clones and horizontal gene transfer. A trade-off between carrying more antibiotic resistance and less virulence-related genes could partially account for the evolutionary advantages featured by successful clones.
Fil: Vignoli, Rafael. Universidad de la República; Uruguay
Fil: García Fulgueiras, Virginia. Universidad de la República; Uruguay
Fil: Cordeiro, Nicolás F.. Universidad de la República; Uruguay
Fil: Bado, Inés. Universidad de la República; Uruguay
Fil: Seija, Verónica. Universidad de la República; Uruguay. Hospital Pasteur de Montevideo; Uruguay
Fil: Aguerrebere, Paula. Universidad de la República; Uruguay
Fil: Laguna, Gabriel. Universidad de la República; Uruguay
Fil: Araújo, Lucía. Universidad de la República; Uruguay
Fil: Bazet, Cristina. Universidad de la República; Uruguay
Fil: Gutkind, Gabriel Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Chabalgoity Rodríguez, José Alejandro. Universidad de la República; Uruguay
Materia
ESBL
PLASMID-MEDIATED QUINOLONE RESISTANCE
VIRULENCE GENES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/99457
Acceder
id CONICETDig_a5ca7865031eec767402e151e6944131
oai_identifier_str oai:ri.conicet.gov.ar:11336/99457
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Extended-spectrum β-lactamases, transferable quinolone resistance, and virulotyping in extra-intestinal E. coli in UruguayVignoli, RafaelGarcía Fulgueiras, VirginiaCordeiro, Nicolás F.Bado, InésSeija, VerónicaAguerrebere, PaulaLaguna, GabrielAraújo, LucíaBazet, CristinaGutkind, Gabriel OsvaldoChabalgoity Rodríguez, José AlejandroESBLPLASMID-MEDIATED QUINOLONE RESISTANCEVIRULENCE GENEShttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Introduction: To characterize extended-spectrum β-lactamases (ESBLs) and plasmid-mediated quinolone resistance (PMQR) genes in Escherichia coli isolates obtained from extra-intestinal samples in three Uruguayan hospitals. Methodology: Fifty-five ESBL-producing E. coli isolates were studied. Virulence genes, ESBLs, and PMQR genes were detected by polymerase chain reaction. ESBL-producing isolates were compared by pulsed-field gel electrophoresis. Multi-locus sequence typing was also performed on 13 selected isolates. Results: Thirty-seven isolates harbored blaCTX-M-15 (67.3%), eight blaCTX-M-2 (14.6%), five blaCTX-M-14 (9.1%), three carried both blaCTX-M-2 and blaCTX-M-14, one blaCTX-M-9, and one blaCTX-M-8. Among the CTX-M-15 producers, 92% belonged to sequence types ST131 and ST405, and carried aac(6’)Ib-cr as well. Isolates harboring blaCTX-M-2, blaCTX-M-14, blaCTX-M-9, or blaCTX-M-8 were found to be genetically unrelated. Conclusions: The successful dissemination of CTX-M-15-producing E.coli isolates seems to be linked to the spreading of high-risk clones and horizontal gene transfer. A trade-off between carrying more antibiotic resistance and less virulence-related genes could partially account for the evolutionary advantages featured by successful clones.Fil: Vignoli, Rafael. Universidad de la República; UruguayFil: García Fulgueiras, Virginia. Universidad de la República; UruguayFil: Cordeiro, Nicolás F.. Universidad de la República; UruguayFil: Bado, Inés. Universidad de la República; UruguayFil: Seija, Verónica. Universidad de la República; Uruguay. Hospital Pasteur de Montevideo; UruguayFil: Aguerrebere, Paula. Universidad de la República; UruguayFil: Laguna, Gabriel. Universidad de la República; UruguayFil: Araújo, Lucía. Universidad de la República; UruguayFil: Bazet, Cristina. Universidad de la República; UruguayFil: Gutkind, Gabriel Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Chabalgoity Rodríguez, José Alejandro. Universidad de la República; UruguayJournal of Infection in Developing Countries2016-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/99457Vignoli, Rafael; García Fulgueiras, Virginia; Cordeiro, Nicolás F.; Bado, Inés; Seija, Verónica; et al.; Extended-spectrum β-lactamases, transferable quinolone resistance, and virulotyping in extra-intestinal E. coli in Uruguay; Journal of Infection in Developing Countries; Journal of Infection in Developing Countries; 10; 1; 1-2016; 43-522036-65901972-2680CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3855/jidc.6918info:eu-repo/semantics/altIdentifier/url/https://jidc.org/index.php/journal/article/view/26829536info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:22:37Zoai:ri.conicet.gov.ar:11336/99457instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:22:37.995CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Extended-spectrum β-lactamases, transferable quinolone resistance, and virulotyping in extra-intestinal E. coli in Uruguay
title Extended-spectrum β-lactamases, transferable quinolone resistance, and virulotyping in extra-intestinal E. coli in Uruguay
spellingShingle Extended-spectrum β-lactamases, transferable quinolone resistance, and virulotyping in extra-intestinal E. coli in Uruguay
Vignoli, Rafael
ESBL
PLASMID-MEDIATED QUINOLONE RESISTANCE
VIRULENCE GENES
title_short Extended-spectrum β-lactamases, transferable quinolone resistance, and virulotyping in extra-intestinal E. coli in Uruguay
title_full Extended-spectrum β-lactamases, transferable quinolone resistance, and virulotyping in extra-intestinal E. coli in Uruguay
title_fullStr Extended-spectrum β-lactamases, transferable quinolone resistance, and virulotyping in extra-intestinal E. coli in Uruguay
title_full_unstemmed Extended-spectrum β-lactamases, transferable quinolone resistance, and virulotyping in extra-intestinal E. coli in Uruguay
title_sort Extended-spectrum β-lactamases, transferable quinolone resistance, and virulotyping in extra-intestinal E. coli in Uruguay
dc.creator.none.fl_str_mv Vignoli, Rafael
García Fulgueiras, Virginia
Cordeiro, Nicolás F.
Bado, Inés
Seija, Verónica
Aguerrebere, Paula
Laguna, Gabriel
Araújo, Lucía
Bazet, Cristina
Gutkind, Gabriel Osvaldo
Chabalgoity Rodríguez, José Alejandro
author Vignoli, Rafael
author_facet Vignoli, Rafael
García Fulgueiras, Virginia
Cordeiro, Nicolás F.
Bado, Inés
Seija, Verónica
Aguerrebere, Paula
Laguna, Gabriel
Araújo, Lucía
Bazet, Cristina
Gutkind, Gabriel Osvaldo
Chabalgoity Rodríguez, José Alejandro
author_role author
author2 García Fulgueiras, Virginia
Cordeiro, Nicolás F.
Bado, Inés
Seija, Verónica
Aguerrebere, Paula
Laguna, Gabriel
Araújo, Lucía
Bazet, Cristina
Gutkind, Gabriel Osvaldo
Chabalgoity Rodríguez, José Alejandro
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ESBL
PLASMID-MEDIATED QUINOLONE RESISTANCE
VIRULENCE GENES
topic ESBL
PLASMID-MEDIATED QUINOLONE RESISTANCE
VIRULENCE GENES
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Introduction: To characterize extended-spectrum β-lactamases (ESBLs) and plasmid-mediated quinolone resistance (PMQR) genes in Escherichia coli isolates obtained from extra-intestinal samples in three Uruguayan hospitals. Methodology: Fifty-five ESBL-producing E. coli isolates were studied. Virulence genes, ESBLs, and PMQR genes were detected by polymerase chain reaction. ESBL-producing isolates were compared by pulsed-field gel electrophoresis. Multi-locus sequence typing was also performed on 13 selected isolates. Results: Thirty-seven isolates harbored blaCTX-M-15 (67.3%), eight blaCTX-M-2 (14.6%), five blaCTX-M-14 (9.1%), three carried both blaCTX-M-2 and blaCTX-M-14, one blaCTX-M-9, and one blaCTX-M-8. Among the CTX-M-15 producers, 92% belonged to sequence types ST131 and ST405, and carried aac(6’)Ib-cr as well. Isolates harboring blaCTX-M-2, blaCTX-M-14, blaCTX-M-9, or blaCTX-M-8 were found to be genetically unrelated. Conclusions: The successful dissemination of CTX-M-15-producing E.coli isolates seems to be linked to the spreading of high-risk clones and horizontal gene transfer. A trade-off between carrying more antibiotic resistance and less virulence-related genes could partially account for the evolutionary advantages featured by successful clones.
Fil: Vignoli, Rafael. Universidad de la República; Uruguay
Fil: García Fulgueiras, Virginia. Universidad de la República; Uruguay
Fil: Cordeiro, Nicolás F.. Universidad de la República; Uruguay
Fil: Bado, Inés. Universidad de la República; Uruguay
Fil: Seija, Verónica. Universidad de la República; Uruguay. Hospital Pasteur de Montevideo; Uruguay
Fil: Aguerrebere, Paula. Universidad de la República; Uruguay
Fil: Laguna, Gabriel. Universidad de la República; Uruguay
Fil: Araújo, Lucía. Universidad de la República; Uruguay
Fil: Bazet, Cristina. Universidad de la República; Uruguay
Fil: Gutkind, Gabriel Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Chabalgoity Rodríguez, José Alejandro. Universidad de la República; Uruguay
description Introduction: To characterize extended-spectrum β-lactamases (ESBLs) and plasmid-mediated quinolone resistance (PMQR) genes in Escherichia coli isolates obtained from extra-intestinal samples in three Uruguayan hospitals. Methodology: Fifty-five ESBL-producing E. coli isolates were studied. Virulence genes, ESBLs, and PMQR genes were detected by polymerase chain reaction. ESBL-producing isolates were compared by pulsed-field gel electrophoresis. Multi-locus sequence typing was also performed on 13 selected isolates. Results: Thirty-seven isolates harbored blaCTX-M-15 (67.3%), eight blaCTX-M-2 (14.6%), five blaCTX-M-14 (9.1%), three carried both blaCTX-M-2 and blaCTX-M-14, one blaCTX-M-9, and one blaCTX-M-8. Among the CTX-M-15 producers, 92% belonged to sequence types ST131 and ST405, and carried aac(6’)Ib-cr as well. Isolates harboring blaCTX-M-2, blaCTX-M-14, blaCTX-M-9, or blaCTX-M-8 were found to be genetically unrelated. Conclusions: The successful dissemination of CTX-M-15-producing E.coli isolates seems to be linked to the spreading of high-risk clones and horizontal gene transfer. A trade-off between carrying more antibiotic resistance and less virulence-related genes could partially account for the evolutionary advantages featured by successful clones.
publishDate 2016
dc.date.none.fl_str_mv 2016-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/99457
Vignoli, Rafael; García Fulgueiras, Virginia; Cordeiro, Nicolás F.; Bado, Inés; Seija, Verónica; et al.; Extended-spectrum β-lactamases, transferable quinolone resistance, and virulotyping in extra-intestinal E. coli in Uruguay; Journal of Infection in Developing Countries; Journal of Infection in Developing Countries; 10; 1; 1-2016; 43-52
2036-6590
1972-2680
CONICET Digital
CONICET
url http://hdl.handle.net/11336/99457
identifier_str_mv Vignoli, Rafael; García Fulgueiras, Virginia; Cordeiro, Nicolás F.; Bado, Inés; Seija, Verónica; et al.; Extended-spectrum β-lactamases, transferable quinolone resistance, and virulotyping in extra-intestinal E. coli in Uruguay; Journal of Infection in Developing Countries; Journal of Infection in Developing Countries; 10; 1; 1-2016; 43-52
2036-6590
1972-2680
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3855/jidc.6918
info:eu-repo/semantics/altIdentifier/url/https://jidc.org/index.php/journal/article/view/26829536
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Journal of Infection in Developing Countries
publisher.none.fl_str_mv Journal of Infection in Developing Countries
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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