Emergence and dominance of CTX-M-15 extended spectrum beta-lactamase among Escherichia coli isolates from children

Autores
Réjiba, Samia; Mercuri, Paola Sandra; Power, Pablo; Kechrid, Amel
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Of forty-seven extended-spectrum cephalosporin-resistant Escherichia coli isolates, collected from children at the Children’s Hospital in 2006 (Tunis, Tunisia), we analyzed 32 isolates that were genotypically different by enterobacterial repetitive intergenic consensus -polymerase chain reaction. For all isolates, the double-disk diffusion test revealed synergy between clavulanate and cefotaxime and/or ceftazidime, suggesting the production of extended-spectrum beta-lactamases. Polymerase chain reaction experiments, performed on plasmid DNA, and sequencing revealed the presence of blaTEM-1B (26 isolates, 81%), blaTEM-34(IRT-6) (3 isolates, 9%), blaSHV-12 (2 isolates, 6%), and blaCTX-M-15 (31 isolates, 97%). Further, the insertion sequence ISEcp1 was found upstream from the blaCTX-M-15 gene in 11 isolates. The bla genes were found alone or in various combinations in a single isolate. blaTEM-1B and blaCTX-M-15 genes were detected in 26 out of the 32 isolates. Three isolates harbored both blaTEM-34(IRT-6) and blaCTX-M-15. blaSHV-12 was identified either alone or with blaCTX-M-15 in a single isolate. Our investigation showed the dominance of CTX-M-type extended-spectrum beta-lactamases, with CTX-M-15 particularly common, and to our best knowledge, this is the first report of the coexistence of CTX-M-15 and IRT-6 in E. coli isolates from children in Tunisia.
Fil: Réjiba, Samia. Université Tunis El-Manar; Túnez. Hôpital d’Enfants; Túnez
Fil: Mercuri, Paola Sandra. Universite de Liege; Bélgica
Fil: Power, Pablo. Universite de Liege; Bélgica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Kechrid, Amel. Hôpital d’Enfants; Túnez
Materia
Microbial Drug Resistance
Mechanisms
Esbl
Molecular Characterization
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/13184
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/13184
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Emergence and dominance of CTX-M-15 extended spectrum beta-lactamase among Escherichia coli isolates from childrenRéjiba, SamiaMercuri, Paola SandraPower, PabloKechrid, AmelMicrobial Drug ResistanceMechanismsEsblMolecular Characterizationhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Of forty-seven extended-spectrum cephalosporin-resistant Escherichia coli isolates, collected from children at the Children’s Hospital in 2006 (Tunis, Tunisia), we analyzed 32 isolates that were genotypically different by enterobacterial repetitive intergenic consensus -polymerase chain reaction. For all isolates, the double-disk diffusion test revealed synergy between clavulanate and cefotaxime and/or ceftazidime, suggesting the production of extended-spectrum beta-lactamases. Polymerase chain reaction experiments, performed on plasmid DNA, and sequencing revealed the presence of blaTEM-1B (26 isolates, 81%), blaTEM-34(IRT-6) (3 isolates, 9%), blaSHV-12 (2 isolates, 6%), and blaCTX-M-15 (31 isolates, 97%). Further, the insertion sequence ISEcp1 was found upstream from the blaCTX-M-15 gene in 11 isolates. The bla genes were found alone or in various combinations in a single isolate. blaTEM-1B and blaCTX-M-15 genes were detected in 26 out of the 32 isolates. Three isolates harbored both blaTEM-34(IRT-6) and blaCTX-M-15. blaSHV-12 was identified either alone or with blaCTX-M-15 in a single isolate. Our investigation showed the dominance of CTX-M-type extended-spectrum beta-lactamases, with CTX-M-15 particularly common, and to our best knowledge, this is the first report of the coexistence of CTX-M-15 and IRT-6 in E. coli isolates from children in Tunisia.Fil: Réjiba, Samia. Université Tunis El-Manar; Túnez. Hôpital d’Enfants; TúnezFil: Mercuri, Paola Sandra. Universite de Liege; BélgicaFil: Power, Pablo. Universite de Liege; Bélgica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Kechrid, Amel. Hôpital d’Enfants; TúnezMary Ann Liebert Inc2011-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/13184Réjiba, Samia; Mercuri, Paola Sandra; Power, Pablo; Kechrid, Amel; Emergence and dominance of CTX-M-15 extended spectrum beta-lactamase among Escherichia coli isolates from children; Mary Ann Liebert Inc; Microbial Drug Resistance-mechanisms Epidemiology And Disease; 17; 2; 4-2011; 135-1401076-6294enginfo:eu-repo/semantics/altIdentifier/url/http://online.liebertpub.com/doi/abs/10.1089/mdr.2010.0098info:eu-repo/semantics/altIdentifier/doi/10.1089/mdr.2010.0098info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:04Zoai:ri.conicet.gov.ar:11336/13184instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:04.235CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Emergence and dominance of CTX-M-15 extended spectrum beta-lactamase among Escherichia coli isolates from children
title Emergence and dominance of CTX-M-15 extended spectrum beta-lactamase among Escherichia coli isolates from children
spellingShingle Emergence and dominance of CTX-M-15 extended spectrum beta-lactamase among Escherichia coli isolates from children
Réjiba, Samia
Microbial Drug Resistance
Mechanisms
Esbl
Molecular Characterization
title_short Emergence and dominance of CTX-M-15 extended spectrum beta-lactamase among Escherichia coli isolates from children
title_full Emergence and dominance of CTX-M-15 extended spectrum beta-lactamase among Escherichia coli isolates from children
title_fullStr Emergence and dominance of CTX-M-15 extended spectrum beta-lactamase among Escherichia coli isolates from children
title_full_unstemmed Emergence and dominance of CTX-M-15 extended spectrum beta-lactamase among Escherichia coli isolates from children
title_sort Emergence and dominance of CTX-M-15 extended spectrum beta-lactamase among Escherichia coli isolates from children
dc.creator.none.fl_str_mv Réjiba, Samia
Mercuri, Paola Sandra
Power, Pablo
Kechrid, Amel
author Réjiba, Samia
author_facet Réjiba, Samia
Mercuri, Paola Sandra
Power, Pablo
Kechrid, Amel
author_role author
author2 Mercuri, Paola Sandra
Power, Pablo
Kechrid, Amel
author2_role author
author
author
dc.subject.none.fl_str_mv Microbial Drug Resistance
Mechanisms
Esbl
Molecular Characterization
topic Microbial Drug Resistance
Mechanisms
Esbl
Molecular Characterization
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Of forty-seven extended-spectrum cephalosporin-resistant Escherichia coli isolates, collected from children at the Children’s Hospital in 2006 (Tunis, Tunisia), we analyzed 32 isolates that were genotypically different by enterobacterial repetitive intergenic consensus -polymerase chain reaction. For all isolates, the double-disk diffusion test revealed synergy between clavulanate and cefotaxime and/or ceftazidime, suggesting the production of extended-spectrum beta-lactamases. Polymerase chain reaction experiments, performed on plasmid DNA, and sequencing revealed the presence of blaTEM-1B (26 isolates, 81%), blaTEM-34(IRT-6) (3 isolates, 9%), blaSHV-12 (2 isolates, 6%), and blaCTX-M-15 (31 isolates, 97%). Further, the insertion sequence ISEcp1 was found upstream from the blaCTX-M-15 gene in 11 isolates. The bla genes were found alone or in various combinations in a single isolate. blaTEM-1B and blaCTX-M-15 genes were detected in 26 out of the 32 isolates. Three isolates harbored both blaTEM-34(IRT-6) and blaCTX-M-15. blaSHV-12 was identified either alone or with blaCTX-M-15 in a single isolate. Our investigation showed the dominance of CTX-M-type extended-spectrum beta-lactamases, with CTX-M-15 particularly common, and to our best knowledge, this is the first report of the coexistence of CTX-M-15 and IRT-6 in E. coli isolates from children in Tunisia.
Fil: Réjiba, Samia. Université Tunis El-Manar; Túnez. Hôpital d’Enfants; Túnez
Fil: Mercuri, Paola Sandra. Universite de Liege; Bélgica
Fil: Power, Pablo. Universite de Liege; Bélgica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Kechrid, Amel. Hôpital d’Enfants; Túnez
description Of forty-seven extended-spectrum cephalosporin-resistant Escherichia coli isolates, collected from children at the Children’s Hospital in 2006 (Tunis, Tunisia), we analyzed 32 isolates that were genotypically different by enterobacterial repetitive intergenic consensus -polymerase chain reaction. For all isolates, the double-disk diffusion test revealed synergy between clavulanate and cefotaxime and/or ceftazidime, suggesting the production of extended-spectrum beta-lactamases. Polymerase chain reaction experiments, performed on plasmid DNA, and sequencing revealed the presence of blaTEM-1B (26 isolates, 81%), blaTEM-34(IRT-6) (3 isolates, 9%), blaSHV-12 (2 isolates, 6%), and blaCTX-M-15 (31 isolates, 97%). Further, the insertion sequence ISEcp1 was found upstream from the blaCTX-M-15 gene in 11 isolates. The bla genes were found alone or in various combinations in a single isolate. blaTEM-1B and blaCTX-M-15 genes were detected in 26 out of the 32 isolates. Three isolates harbored both blaTEM-34(IRT-6) and blaCTX-M-15. blaSHV-12 was identified either alone or with blaCTX-M-15 in a single isolate. Our investigation showed the dominance of CTX-M-type extended-spectrum beta-lactamases, with CTX-M-15 particularly common, and to our best knowledge, this is the first report of the coexistence of CTX-M-15 and IRT-6 in E. coli isolates from children in Tunisia.
publishDate 2011
dc.date.none.fl_str_mv 2011-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13184
Réjiba, Samia; Mercuri, Paola Sandra; Power, Pablo; Kechrid, Amel; Emergence and dominance of CTX-M-15 extended spectrum beta-lactamase among Escherichia coli isolates from children; Mary Ann Liebert Inc; Microbial Drug Resistance-mechanisms Epidemiology And Disease; 17; 2; 4-2011; 135-140
1076-6294
url http://hdl.handle.net/11336/13184
identifier_str_mv Réjiba, Samia; Mercuri, Paola Sandra; Power, Pablo; Kechrid, Amel; Emergence and dominance of CTX-M-15 extended spectrum beta-lactamase among Escherichia coli isolates from children; Mary Ann Liebert Inc; Microbial Drug Resistance-mechanisms Epidemiology And Disease; 17; 2; 4-2011; 135-140
1076-6294
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://online.liebertpub.com/doi/abs/10.1089/mdr.2010.0098
info:eu-repo/semantics/altIdentifier/doi/10.1089/mdr.2010.0098
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Mary Ann Liebert Inc
publisher.none.fl_str_mv Mary Ann Liebert Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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