Involvement of microenvironment factors in the PTHrP effect on the aggressive behavior of colorectal cancer cells

Autores
Novoa Díaz, María Belén; Carriere, Pedro Matias; Calvo, Natalia Graciela; Gentili, Claudia Rosana
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
PTHrP is a factor from the tumor and its microenvironment that has been associated with the aggressiveness of different types of cancer. Colorectal cancer (CRC) is a heterogeneous disease where microenvironment and tumor factors act together leading to the progression of the tumor towards advanced stages. Previously, we observed that in the cell line HCT116 derived from human CRC, the treatment with exogenous PTHrP stimulates its transcription and also modulates the expression of markers associated with aggressive behavior such as E-Cadherin, FAK, Met and CD44. In addition PTHrP activates mitogenic pathways in HMEC-1 endothelial cells. To understand the PTHrP role in tumor progression through its influence on microenvironment cells, in this work we first evaluated in HMEC cells the effects of PTHrP in the regulation of cytokines expression and we observed by Western blot analysis that the treatment with the hormone for 1 and 16 h increases protein levels of TGF-β, which is a protumoral factor known to be closely linked to PTHrP. Then we evaluate the effect of the conditioned medium (CM) from the HMEC-1 cells treated with PTHrP on the intestinal tumor cells HCT116. According with our previous results regarding to PTHrP direct action on HCT116 cells, the CM also induces an increase in the protein expression of FAK and CD44 in a time-dependent manner, and decreases the protein level of E-cadherin but at shorter times respect to the direct action of PTHrP. Previously we found that PTHrP increases the protein levels of Met in HCT116 cells and herein we observed by CM treatment the same effect but at longer times of exposure. The results of this work allow us to postulate a mechanism based in the action of PTHrP on tumor niche cells leading to the subsequent release of factors to the environment that could contribute to its protumoral effect.
Fil: Novoa Díaz, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Carriere, Pedro Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Calvo, Natalia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Gentili, Claudia Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Reunión anual de sociedades de biociencia 2019: LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); LI Reunión Anual de la Asociación Argentina de Farmacología Experimental (SAFE); XXI Reunión Anual de la Sociedad Argentina de Biología (SAB); XXXI Reunión Anual de la Sociedad Argentina de Protozoología (SAP); IX Reunión Anual de la Asociación Argentina de Nanomedicinas (NANOMED-ar); VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (AACyTAL)
Mar del Plata
Argentina
Sociedad argentina de investigación clínica
Asociación argentina de farmacología experimental
Sociedad argentina de biología
Sociedad argentina de protozoología
Asociación argentina de nanomedicinas
Asociación argentina de ciencia y tecnología de animales de laboratorio
Materia
PTHrP
COLORECTAL CANCER
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/200339
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Involvement of microenvironment factors in the PTHrP effect on the aggressive behavior of colorectal cancer cellsNovoa Díaz, María BelénCarriere, Pedro MatiasCalvo, Natalia GracielaGentili, Claudia RosanaPTHrPCOLORECTAL CANCERhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1PTHrP is a factor from the tumor and its microenvironment that has been associated with the aggressiveness of different types of cancer. Colorectal cancer (CRC) is a heterogeneous disease where microenvironment and tumor factors act together leading to the progression of the tumor towards advanced stages. Previously, we observed that in the cell line HCT116 derived from human CRC, the treatment with exogenous PTHrP stimulates its transcription and also modulates the expression of markers associated with aggressive behavior such as E-Cadherin, FAK, Met and CD44. In addition PTHrP activates mitogenic pathways in HMEC-1 endothelial cells. To understand the PTHrP role in tumor progression through its influence on microenvironment cells, in this work we first evaluated in HMEC cells the effects of PTHrP in the regulation of cytokines expression and we observed by Western blot analysis that the treatment with the hormone for 1 and 16 h increases protein levels of TGF-β, which is a protumoral factor known to be closely linked to PTHrP. Then we evaluate the effect of the conditioned medium (CM) from the HMEC-1 cells treated with PTHrP on the intestinal tumor cells HCT116. According with our previous results regarding to PTHrP direct action on HCT116 cells, the CM also induces an increase in the protein expression of FAK and CD44 in a time-dependent manner, and decreases the protein level of E-cadherin but at shorter times respect to the direct action of PTHrP. Previously we found that PTHrP increases the protein levels of Met in HCT116 cells and herein we observed by CM treatment the same effect but at longer times of exposure. The results of this work allow us to postulate a mechanism based in the action of PTHrP on tumor niche cells leading to the subsequent release of factors to the environment that could contribute to its protumoral effect.Fil: Novoa Díaz, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Carriere, Pedro Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Calvo, Natalia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Gentili, Claudia Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaReunión anual de sociedades de biociencia 2019: LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); LI Reunión Anual de la Asociación Argentina de Farmacología Experimental (SAFE); XXI Reunión Anual de la Sociedad Argentina de Biología (SAB); XXXI Reunión Anual de la Sociedad Argentina de Protozoología (SAP); IX Reunión Anual de la Asociación Argentina de Nanomedicinas (NANOMED-ar); VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (AACyTAL)Mar del PlataArgentinaSociedad argentina de investigación clínicaAsociación argentina de farmacología experimentalSociedad argentina de biologíaSociedad argentina de protozoologíaAsociación argentina de nanomedicinasAsociación argentina de ciencia y tecnología de animales de laboratorioMedicina (Buenos Aires)Costas, Mónica2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/200339Involvement of microenvironment factors in the PTHrP effect on the aggressive behavior of colorectal cancer cells; Reunión anual de sociedades de biociencia 2019: LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); LI Reunión Anual de la Asociación Argentina de Farmacología Experimental (SAFE); XXI Reunión Anual de la Sociedad Argentina de Biología (SAB); XXXI Reunión Anual de la Sociedad Argentina de Protozoología (SAP); IX Reunión Anual de la Asociación Argentina de Nanomedicinas (NANOMED-ar); VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (AACyTAL); Mar del Plata; Argentina; 2019; 131-1310025-7680CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol79-19/s4/vol79_s4.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:45:35Zoai:ri.conicet.gov.ar:11336/200339instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:45:35.323CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Involvement of microenvironment factors in the PTHrP effect on the aggressive behavior of colorectal cancer cells
title Involvement of microenvironment factors in the PTHrP effect on the aggressive behavior of colorectal cancer cells
spellingShingle Involvement of microenvironment factors in the PTHrP effect on the aggressive behavior of colorectal cancer cells
Novoa Díaz, María Belén
PTHrP
COLORECTAL CANCER
title_short Involvement of microenvironment factors in the PTHrP effect on the aggressive behavior of colorectal cancer cells
title_full Involvement of microenvironment factors in the PTHrP effect on the aggressive behavior of colorectal cancer cells
title_fullStr Involvement of microenvironment factors in the PTHrP effect on the aggressive behavior of colorectal cancer cells
title_full_unstemmed Involvement of microenvironment factors in the PTHrP effect on the aggressive behavior of colorectal cancer cells
title_sort Involvement of microenvironment factors in the PTHrP effect on the aggressive behavior of colorectal cancer cells
dc.creator.none.fl_str_mv Novoa Díaz, María Belén
Carriere, Pedro Matias
Calvo, Natalia Graciela
Gentili, Claudia Rosana
author Novoa Díaz, María Belén
author_facet Novoa Díaz, María Belén
Carriere, Pedro Matias
Calvo, Natalia Graciela
Gentili, Claudia Rosana
author_role author
author2 Carriere, Pedro Matias
Calvo, Natalia Graciela
Gentili, Claudia Rosana
author2_role author
author
author
dc.contributor.none.fl_str_mv Costas, Mónica
dc.subject.none.fl_str_mv PTHrP
COLORECTAL CANCER
topic PTHrP
COLORECTAL CANCER
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv PTHrP is a factor from the tumor and its microenvironment that has been associated with the aggressiveness of different types of cancer. Colorectal cancer (CRC) is a heterogeneous disease where microenvironment and tumor factors act together leading to the progression of the tumor towards advanced stages. Previously, we observed that in the cell line HCT116 derived from human CRC, the treatment with exogenous PTHrP stimulates its transcription and also modulates the expression of markers associated with aggressive behavior such as E-Cadherin, FAK, Met and CD44. In addition PTHrP activates mitogenic pathways in HMEC-1 endothelial cells. To understand the PTHrP role in tumor progression through its influence on microenvironment cells, in this work we first evaluated in HMEC cells the effects of PTHrP in the regulation of cytokines expression and we observed by Western blot analysis that the treatment with the hormone for 1 and 16 h increases protein levels of TGF-β, which is a protumoral factor known to be closely linked to PTHrP. Then we evaluate the effect of the conditioned medium (CM) from the HMEC-1 cells treated with PTHrP on the intestinal tumor cells HCT116. According with our previous results regarding to PTHrP direct action on HCT116 cells, the CM also induces an increase in the protein expression of FAK and CD44 in a time-dependent manner, and decreases the protein level of E-cadherin but at shorter times respect to the direct action of PTHrP. Previously we found that PTHrP increases the protein levels of Met in HCT116 cells and herein we observed by CM treatment the same effect but at longer times of exposure. The results of this work allow us to postulate a mechanism based in the action of PTHrP on tumor niche cells leading to the subsequent release of factors to the environment that could contribute to its protumoral effect.
Fil: Novoa Díaz, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Carriere, Pedro Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Calvo, Natalia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Gentili, Claudia Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Reunión anual de sociedades de biociencia 2019: LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); LI Reunión Anual de la Asociación Argentina de Farmacología Experimental (SAFE); XXI Reunión Anual de la Sociedad Argentina de Biología (SAB); XXXI Reunión Anual de la Sociedad Argentina de Protozoología (SAP); IX Reunión Anual de la Asociación Argentina de Nanomedicinas (NANOMED-ar); VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (AACyTAL)
Mar del Plata
Argentina
Sociedad argentina de investigación clínica
Asociación argentina de farmacología experimental
Sociedad argentina de biología
Sociedad argentina de protozoología
Asociación argentina de nanomedicinas
Asociación argentina de ciencia y tecnología de animales de laboratorio
description PTHrP is a factor from the tumor and its microenvironment that has been associated with the aggressiveness of different types of cancer. Colorectal cancer (CRC) is a heterogeneous disease where microenvironment and tumor factors act together leading to the progression of the tumor towards advanced stages. Previously, we observed that in the cell line HCT116 derived from human CRC, the treatment with exogenous PTHrP stimulates its transcription and also modulates the expression of markers associated with aggressive behavior such as E-Cadherin, FAK, Met and CD44. In addition PTHrP activates mitogenic pathways in HMEC-1 endothelial cells. To understand the PTHrP role in tumor progression through its influence on microenvironment cells, in this work we first evaluated in HMEC cells the effects of PTHrP in the regulation of cytokines expression and we observed by Western blot analysis that the treatment with the hormone for 1 and 16 h increases protein levels of TGF-β, which is a protumoral factor known to be closely linked to PTHrP. Then we evaluate the effect of the conditioned medium (CM) from the HMEC-1 cells treated with PTHrP on the intestinal tumor cells HCT116. According with our previous results regarding to PTHrP direct action on HCT116 cells, the CM also induces an increase in the protein expression of FAK and CD44 in a time-dependent manner, and decreases the protein level of E-cadherin but at shorter times respect to the direct action of PTHrP. Previously we found that PTHrP increases the protein levels of Met in HCT116 cells and herein we observed by CM treatment the same effect but at longer times of exposure. The results of this work allow us to postulate a mechanism based in the action of PTHrP on tumor niche cells leading to the subsequent release of factors to the environment that could contribute to its protumoral effect.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
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info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/200339
Involvement of microenvironment factors in the PTHrP effect on the aggressive behavior of colorectal cancer cells; Reunión anual de sociedades de biociencia 2019: LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); LI Reunión Anual de la Asociación Argentina de Farmacología Experimental (SAFE); XXI Reunión Anual de la Sociedad Argentina de Biología (SAB); XXXI Reunión Anual de la Sociedad Argentina de Protozoología (SAP); IX Reunión Anual de la Asociación Argentina de Nanomedicinas (NANOMED-ar); VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (AACyTAL); Mar del Plata; Argentina; 2019; 131-131
0025-7680
CONICET Digital
CONICET
url http://hdl.handle.net/11336/200339
identifier_str_mv Involvement of microenvironment factors in the PTHrP effect on the aggressive behavior of colorectal cancer cells; Reunión anual de sociedades de biociencia 2019: LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); LI Reunión Anual de la Asociación Argentina de Farmacología Experimental (SAFE); XXI Reunión Anual de la Sociedad Argentina de Biología (SAB); XXXI Reunión Anual de la Sociedad Argentina de Protozoología (SAP); IX Reunión Anual de la Asociación Argentina de Nanomedicinas (NANOMED-ar); VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (AACyTAL); Mar del Plata; Argentina; 2019; 131-131
0025-7680
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Medicina (Buenos Aires)
publisher.none.fl_str_mv Medicina (Buenos Aires)
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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