Evaluation of Anti-Inflammatory Activity of Pseudananas macrodontes (Morr.) Harms (Bromeliaceae) Fruit Extract in Rats

Autores
Errasti, María Eugenia; Caffini, Nestor Oscar; Pelzer, Lilian Eugenia; Rotelli, Alejandra Ester
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Several species of the family Bromeliaceae are characterized by the production of proteases in unusual amounts, especially in fruits. Bromelain, an extract rich in cysteine endopeptidases obtained from Ananas comosus L., and a few other proteases have been used as anti-infl ammatory agents for some years, but bromelain is still mainly being used as alternative and/or complementary therapy to the treatment with glucocorticoids, nonsteroidal antirheumatics, and immunomodulators. In this study, the anti-infl ammatory action of a partially purifi ed extract from Pseudananas macrodontes (Morr.) Harms fruits (PPEPm) is presented, whose main components are cysteine endopeptidases. The effect of PPEPm was assessed in carrageenan-induced and serotonin-induced rat paw edema, as well as in the cotton pellet granuloma model. Doses with equal proteolytic activity of PPEPm and bromelain produced signifi cantly similar anti-infl ammatory responses in the acute infl ammatory models assayed, supporting the hypothesis that proteolytic activity could be responsible for the anti-infl ammatory action. On the contrary, comparable anti-infl ammatory effects of PPEPm and bromelain in the chronic infl ammatory assay required a much lower proteolytic activity content of PPEPm, which could be due to a differential affi nity for the protein target involved in this process.
Fil: Errasti, María Eugenia. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biologicas. Laboratorio de Investigacion de Proteinas Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Caffini, Nestor Oscar. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biologicas. Laboratorio de Investigacion de Proteinas Vegetales; Argentina
Fil: Pelzer, Lilian Eugenia. Universidad Nacional de San Luis; Argentina
Fil: Rotelli, Alejandra Ester. Universidad Nacional de San Luis; Argentina
Materia
Anti-Infl Ammatory
Pseudananas Macrodontes
Plant Proteases
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24639
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Evaluation of Anti-Inflammatory Activity of Pseudananas macrodontes (Morr.) Harms (Bromeliaceae) Fruit Extract in RatsErrasti, María EugeniaCaffini, Nestor OscarPelzer, Lilian EugeniaRotelli, Alejandra EsterAnti-Infl AmmatoryPseudananas MacrodontesPlant Proteaseshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Several species of the family Bromeliaceae are characterized by the production of proteases in unusual amounts, especially in fruits. Bromelain, an extract rich in cysteine endopeptidases obtained from Ananas comosus L., and a few other proteases have been used as anti-infl ammatory agents for some years, but bromelain is still mainly being used as alternative and/or complementary therapy to the treatment with glucocorticoids, nonsteroidal antirheumatics, and immunomodulators. In this study, the anti-infl ammatory action of a partially purifi ed extract from Pseudananas macrodontes (Morr.) Harms fruits (PPEPm) is presented, whose main components are cysteine endopeptidases. The effect of PPEPm was assessed in carrageenan-induced and serotonin-induced rat paw edema, as well as in the cotton pellet granuloma model. Doses with equal proteolytic activity of PPEPm and bromelain produced signifi cantly similar anti-infl ammatory responses in the acute infl ammatory models assayed, supporting the hypothesis that proteolytic activity could be responsible for the anti-infl ammatory action. On the contrary, comparable anti-infl ammatory effects of PPEPm and bromelain in the chronic infl ammatory assay required a much lower proteolytic activity content of PPEPm, which could be due to a differential affi nity for the protein target involved in this process.Fil: Errasti, María Eugenia. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biologicas. Laboratorio de Investigacion de Proteinas Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Caffini, Nestor Oscar. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biologicas. Laboratorio de Investigacion de Proteinas Vegetales; ArgentinaFil: Pelzer, Lilian Eugenia. Universidad Nacional de San Luis; ArgentinaFil: Rotelli, Alejandra Ester. Universidad Nacional de San Luis; ArgentinaVerlag Z Naturforsch2014-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24639Errasti, María Eugenia; Caffini, Nestor Oscar; Pelzer, Lilian Eugenia; Rotelli, Alejandra Ester; Evaluation of Anti-Inflammatory Activity of Pseudananas macrodontes (Morr.) Harms (Bromeliaceae) Fruit Extract in Rats; Verlag Z Naturforsch; Zeitschrift Fur Naturforschung C-a Journal Of Biosciences; 68; 11-12; 1-2014; 445-4520939-5075CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.znaturforsch.com/ac/v68c/c68c.htminfo:eu-repo/semantics/altIdentifier/url/http://www.znaturforsch.com/s68c/s68c0445.pdfinfo:eu-repo/semantics/altIdentifier/url/http://www.znaturforsch.com/ac/v68c/68c0445.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:43:44Zoai:ri.conicet.gov.ar:11336/24639instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:43:44.583CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of Anti-Inflammatory Activity of Pseudananas macrodontes (Morr.) Harms (Bromeliaceae) Fruit Extract in Rats
title Evaluation of Anti-Inflammatory Activity of Pseudananas macrodontes (Morr.) Harms (Bromeliaceae) Fruit Extract in Rats
spellingShingle Evaluation of Anti-Inflammatory Activity of Pseudananas macrodontes (Morr.) Harms (Bromeliaceae) Fruit Extract in Rats
Errasti, María Eugenia
Anti-Infl Ammatory
Pseudananas Macrodontes
Plant Proteases
title_short Evaluation of Anti-Inflammatory Activity of Pseudananas macrodontes (Morr.) Harms (Bromeliaceae) Fruit Extract in Rats
title_full Evaluation of Anti-Inflammatory Activity of Pseudananas macrodontes (Morr.) Harms (Bromeliaceae) Fruit Extract in Rats
title_fullStr Evaluation of Anti-Inflammatory Activity of Pseudananas macrodontes (Morr.) Harms (Bromeliaceae) Fruit Extract in Rats
title_full_unstemmed Evaluation of Anti-Inflammatory Activity of Pseudananas macrodontes (Morr.) Harms (Bromeliaceae) Fruit Extract in Rats
title_sort Evaluation of Anti-Inflammatory Activity of Pseudananas macrodontes (Morr.) Harms (Bromeliaceae) Fruit Extract in Rats
dc.creator.none.fl_str_mv Errasti, María Eugenia
Caffini, Nestor Oscar
Pelzer, Lilian Eugenia
Rotelli, Alejandra Ester
author Errasti, María Eugenia
author_facet Errasti, María Eugenia
Caffini, Nestor Oscar
Pelzer, Lilian Eugenia
Rotelli, Alejandra Ester
author_role author
author2 Caffini, Nestor Oscar
Pelzer, Lilian Eugenia
Rotelli, Alejandra Ester
author2_role author
author
author
dc.subject.none.fl_str_mv Anti-Infl Ammatory
Pseudananas Macrodontes
Plant Proteases
topic Anti-Infl Ammatory
Pseudananas Macrodontes
Plant Proteases
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Several species of the family Bromeliaceae are characterized by the production of proteases in unusual amounts, especially in fruits. Bromelain, an extract rich in cysteine endopeptidases obtained from Ananas comosus L., and a few other proteases have been used as anti-infl ammatory agents for some years, but bromelain is still mainly being used as alternative and/or complementary therapy to the treatment with glucocorticoids, nonsteroidal antirheumatics, and immunomodulators. In this study, the anti-infl ammatory action of a partially purifi ed extract from Pseudananas macrodontes (Morr.) Harms fruits (PPEPm) is presented, whose main components are cysteine endopeptidases. The effect of PPEPm was assessed in carrageenan-induced and serotonin-induced rat paw edema, as well as in the cotton pellet granuloma model. Doses with equal proteolytic activity of PPEPm and bromelain produced signifi cantly similar anti-infl ammatory responses in the acute infl ammatory models assayed, supporting the hypothesis that proteolytic activity could be responsible for the anti-infl ammatory action. On the contrary, comparable anti-infl ammatory effects of PPEPm and bromelain in the chronic infl ammatory assay required a much lower proteolytic activity content of PPEPm, which could be due to a differential affi nity for the protein target involved in this process.
Fil: Errasti, María Eugenia. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biologicas. Laboratorio de Investigacion de Proteinas Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Caffini, Nestor Oscar. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biologicas. Laboratorio de Investigacion de Proteinas Vegetales; Argentina
Fil: Pelzer, Lilian Eugenia. Universidad Nacional de San Luis; Argentina
Fil: Rotelli, Alejandra Ester. Universidad Nacional de San Luis; Argentina
description Several species of the family Bromeliaceae are characterized by the production of proteases in unusual amounts, especially in fruits. Bromelain, an extract rich in cysteine endopeptidases obtained from Ananas comosus L., and a few other proteases have been used as anti-infl ammatory agents for some years, but bromelain is still mainly being used as alternative and/or complementary therapy to the treatment with glucocorticoids, nonsteroidal antirheumatics, and immunomodulators. In this study, the anti-infl ammatory action of a partially purifi ed extract from Pseudananas macrodontes (Morr.) Harms fruits (PPEPm) is presented, whose main components are cysteine endopeptidases. The effect of PPEPm was assessed in carrageenan-induced and serotonin-induced rat paw edema, as well as in the cotton pellet granuloma model. Doses with equal proteolytic activity of PPEPm and bromelain produced signifi cantly similar anti-infl ammatory responses in the acute infl ammatory models assayed, supporting the hypothesis that proteolytic activity could be responsible for the anti-infl ammatory action. On the contrary, comparable anti-infl ammatory effects of PPEPm and bromelain in the chronic infl ammatory assay required a much lower proteolytic activity content of PPEPm, which could be due to a differential affi nity for the protein target involved in this process.
publishDate 2014
dc.date.none.fl_str_mv 2014-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24639
Errasti, María Eugenia; Caffini, Nestor Oscar; Pelzer, Lilian Eugenia; Rotelli, Alejandra Ester; Evaluation of Anti-Inflammatory Activity of Pseudananas macrodontes (Morr.) Harms (Bromeliaceae) Fruit Extract in Rats; Verlag Z Naturforsch; Zeitschrift Fur Naturforschung C-a Journal Of Biosciences; 68; 11-12; 1-2014; 445-452
0939-5075
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24639
identifier_str_mv Errasti, María Eugenia; Caffini, Nestor Oscar; Pelzer, Lilian Eugenia; Rotelli, Alejandra Ester; Evaluation of Anti-Inflammatory Activity of Pseudananas macrodontes (Morr.) Harms (Bromeliaceae) Fruit Extract in Rats; Verlag Z Naturforsch; Zeitschrift Fur Naturforschung C-a Journal Of Biosciences; 68; 11-12; 1-2014; 445-452
0939-5075
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.znaturforsch.com/ac/v68c/c68c.htm
info:eu-repo/semantics/altIdentifier/url/http://www.znaturforsch.com/s68c/s68c0445.pdf
info:eu-repo/semantics/altIdentifier/url/http://www.znaturforsch.com/ac/v68c/68c0445.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Verlag Z Naturforsch
publisher.none.fl_str_mv Verlag Z Naturforsch
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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