TRPC3 channels critically regulate hippocampal excitability and contextual fear memory

Autores
Neuner, Sarah M.; Wilmott, Lynda A.; Hope, Kevin A.; Hoffmann, Brian; Chong, Jayhong A.; Abramowitz, Joel; Birnbaumer, Lutz; O'Connell, Kristen M.; Tryba, Andrew K.; Greene, Andrew S.; Savio Chan, C.; Kaczorowski, Catherine C.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Memory formation requires de novo protein synthesis, and memory disorders may result from misregulated synthesis of critical proteins that remain largely unidentified. Plasma membrane ion channels and receptors are likely candidates given their role in regulating neuron excitability, a candidate memory mechanism. Here we conduct targeted molecular monitoring and quantitation of hippocampal plasma membrane proteins from mice with intact or impaired contextual fear memory to identify putative candidates. Here we report contextual fear memory deficits correspond to increased Trpc3 gene and protein expression, and demonstrate TRPC3 regulates hippocampal neuron excitability associated with memory function. These data provide a mechanistic explanation for enhanced contextual fear memory reported herein following knockdown of TRPC3 in hippocampus. Collectively, TRPC3 modulates memory and may be a feasible target to enhance memory and treat memory disorders.
Fil: Neuner, Sarah M.. The University of Tennessee Health Science Center. Department of Anatomy and Neurobiology; Estados Unidos
Fil: Wilmott, Lynda A.. The University of Tennessee Health Science Center. Department of Anatomy and Neurobiology; Estados Unidos
Fil: Hope, Kevin A.. The University of Tennessee Health Science Center. Department of Anatomy and Neurobiology; Estados Unidos
Fil: Hoffmann, Brian. Medical College of Wisconsin. Department of Biotechnology and Bioengineering; Estados Unidos
Fil: Chong, Jayhong A.. MA. Hydra Biosciences, Cambridge; Estados Unidos
Fil: Abramowitz, Joel. Research Triangle Park. Laboratory of Neurobiology. National Institute of Environmental Health Sciences; Estados Unidos
Fil: Birnbaumer, Lutz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Research Triangle Park. Laboratory of Neurobiology. National Institute of Environmental Health Sciences; Estados Unidos
Fil: O'Connell, Kristen M.. University of Tennessee Health Science Center. Department of Physiology; Estados Unidos
Fil: Savio, Chan C.. Northwestern Fienberg School of Medicine. Department of Physiology; Estados Unidos
Fil: Kaczorowski, Catherine C.. The University of Tennessee Health Science Center. Department of Anatomy and Neurobiology; Estados Unidos
Materia
AFTERHYPERPOLOARIZATION
AGING
HIPPOCAMPUS
MEMORY
PROTEOMICS
TRANSIENT RECEPTOR POTENTIAL CATION CHANNEL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/94231
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/94231
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling TRPC3 channels critically regulate hippocampal excitability and contextual fear memoryNeuner, Sarah M.Wilmott, Lynda A.Hope, Kevin A.Hoffmann, BrianChong, Jayhong A.Abramowitz, JoelBirnbaumer, LutzO'Connell, Kristen M.Tryba, Andrew K.Greene, Andrew S.Savio Chan, C.Kaczorowski, Catherine C.AFTERHYPERPOLOARIZATIONAGINGHIPPOCAMPUSMEMORYPROTEOMICSTRANSIENT RECEPTOR POTENTIAL CATION CHANNELhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Memory formation requires de novo protein synthesis, and memory disorders may result from misregulated synthesis of critical proteins that remain largely unidentified. Plasma membrane ion channels and receptors are likely candidates given their role in regulating neuron excitability, a candidate memory mechanism. Here we conduct targeted molecular monitoring and quantitation of hippocampal plasma membrane proteins from mice with intact or impaired contextual fear memory to identify putative candidates. Here we report contextual fear memory deficits correspond to increased Trpc3 gene and protein expression, and demonstrate TRPC3 regulates hippocampal neuron excitability associated with memory function. These data provide a mechanistic explanation for enhanced contextual fear memory reported herein following knockdown of TRPC3 in hippocampus. Collectively, TRPC3 modulates memory and may be a feasible target to enhance memory and treat memory disorders.Fil: Neuner, Sarah M.. The University of Tennessee Health Science Center. Department of Anatomy and Neurobiology; Estados UnidosFil: Wilmott, Lynda A.. The University of Tennessee Health Science Center. Department of Anatomy and Neurobiology; Estados UnidosFil: Hope, Kevin A.. The University of Tennessee Health Science Center. Department of Anatomy and Neurobiology; Estados UnidosFil: Hoffmann, Brian. Medical College of Wisconsin. Department of Biotechnology and Bioengineering; Estados UnidosFil: Chong, Jayhong A.. MA. Hydra Biosciences, Cambridge; Estados UnidosFil: Abramowitz, Joel. Research Triangle Park. Laboratory of Neurobiology. National Institute of Environmental Health Sciences; Estados UnidosFil: Birnbaumer, Lutz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Research Triangle Park. Laboratory of Neurobiology. National Institute of Environmental Health Sciences; Estados UnidosFil: O'Connell, Kristen M.. University of Tennessee Health Science Center. Department of Physiology; Estados UnidosFil: Savio, Chan C.. Northwestern Fienberg School of Medicine. Department of Physiology; Estados UnidosFil: Kaczorowski, Catherine C.. The University of Tennessee Health Science Center. Department of Anatomy and Neurobiology; Estados UnidosElsevier Science2015-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/94231Neuner, Sarah M.; Wilmott, Lynda A.; Hope, Kevin A.; Hoffmann, Brian; Chong, Jayhong A.; et al.; TRPC3 channels critically regulate hippocampal excitability and contextual fear memory; Elsevier Science; Behavioural Brain Research; 281; 3-2015; 69-770166-4328CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0166432814008122info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbr.2014.12.018info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:02:43Zoai:ri.conicet.gov.ar:11336/94231instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:02:43.539CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv TRPC3 channels critically regulate hippocampal excitability and contextual fear memory
title TRPC3 channels critically regulate hippocampal excitability and contextual fear memory
spellingShingle TRPC3 channels critically regulate hippocampal excitability and contextual fear memory
Neuner, Sarah M.
AFTERHYPERPOLOARIZATION
AGING
HIPPOCAMPUS
MEMORY
PROTEOMICS
TRANSIENT RECEPTOR POTENTIAL CATION CHANNEL
title_short TRPC3 channels critically regulate hippocampal excitability and contextual fear memory
title_full TRPC3 channels critically regulate hippocampal excitability and contextual fear memory
title_fullStr TRPC3 channels critically regulate hippocampal excitability and contextual fear memory
title_full_unstemmed TRPC3 channels critically regulate hippocampal excitability and contextual fear memory
title_sort TRPC3 channels critically regulate hippocampal excitability and contextual fear memory
dc.creator.none.fl_str_mv Neuner, Sarah M.
Wilmott, Lynda A.
Hope, Kevin A.
Hoffmann, Brian
Chong, Jayhong A.
Abramowitz, Joel
Birnbaumer, Lutz
O'Connell, Kristen M.
Tryba, Andrew K.
Greene, Andrew S.
Savio Chan, C.
Kaczorowski, Catherine C.
author Neuner, Sarah M.
author_facet Neuner, Sarah M.
Wilmott, Lynda A.
Hope, Kevin A.
Hoffmann, Brian
Chong, Jayhong A.
Abramowitz, Joel
Birnbaumer, Lutz
O'Connell, Kristen M.
Tryba, Andrew K.
Greene, Andrew S.
Savio Chan, C.
Kaczorowski, Catherine C.
author_role author
author2 Wilmott, Lynda A.
Hope, Kevin A.
Hoffmann, Brian
Chong, Jayhong A.
Abramowitz, Joel
Birnbaumer, Lutz
O'Connell, Kristen M.
Tryba, Andrew K.
Greene, Andrew S.
Savio Chan, C.
Kaczorowski, Catherine C.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv AFTERHYPERPOLOARIZATION
AGING
HIPPOCAMPUS
MEMORY
PROTEOMICS
TRANSIENT RECEPTOR POTENTIAL CATION CHANNEL
topic AFTERHYPERPOLOARIZATION
AGING
HIPPOCAMPUS
MEMORY
PROTEOMICS
TRANSIENT RECEPTOR POTENTIAL CATION CHANNEL
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Memory formation requires de novo protein synthesis, and memory disorders may result from misregulated synthesis of critical proteins that remain largely unidentified. Plasma membrane ion channels and receptors are likely candidates given their role in regulating neuron excitability, a candidate memory mechanism. Here we conduct targeted molecular monitoring and quantitation of hippocampal plasma membrane proteins from mice with intact or impaired contextual fear memory to identify putative candidates. Here we report contextual fear memory deficits correspond to increased Trpc3 gene and protein expression, and demonstrate TRPC3 regulates hippocampal neuron excitability associated with memory function. These data provide a mechanistic explanation for enhanced contextual fear memory reported herein following knockdown of TRPC3 in hippocampus. Collectively, TRPC3 modulates memory and may be a feasible target to enhance memory and treat memory disorders.
Fil: Neuner, Sarah M.. The University of Tennessee Health Science Center. Department of Anatomy and Neurobiology; Estados Unidos
Fil: Wilmott, Lynda A.. The University of Tennessee Health Science Center. Department of Anatomy and Neurobiology; Estados Unidos
Fil: Hope, Kevin A.. The University of Tennessee Health Science Center. Department of Anatomy and Neurobiology; Estados Unidos
Fil: Hoffmann, Brian. Medical College of Wisconsin. Department of Biotechnology and Bioengineering; Estados Unidos
Fil: Chong, Jayhong A.. MA. Hydra Biosciences, Cambridge; Estados Unidos
Fil: Abramowitz, Joel. Research Triangle Park. Laboratory of Neurobiology. National Institute of Environmental Health Sciences; Estados Unidos
Fil: Birnbaumer, Lutz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Research Triangle Park. Laboratory of Neurobiology. National Institute of Environmental Health Sciences; Estados Unidos
Fil: O'Connell, Kristen M.. University of Tennessee Health Science Center. Department of Physiology; Estados Unidos
Fil: Savio, Chan C.. Northwestern Fienberg School of Medicine. Department of Physiology; Estados Unidos
Fil: Kaczorowski, Catherine C.. The University of Tennessee Health Science Center. Department of Anatomy and Neurobiology; Estados Unidos
description Memory formation requires de novo protein synthesis, and memory disorders may result from misregulated synthesis of critical proteins that remain largely unidentified. Plasma membrane ion channels and receptors are likely candidates given their role in regulating neuron excitability, a candidate memory mechanism. Here we conduct targeted molecular monitoring and quantitation of hippocampal plasma membrane proteins from mice with intact or impaired contextual fear memory to identify putative candidates. Here we report contextual fear memory deficits correspond to increased Trpc3 gene and protein expression, and demonstrate TRPC3 regulates hippocampal neuron excitability associated with memory function. These data provide a mechanistic explanation for enhanced contextual fear memory reported herein following knockdown of TRPC3 in hippocampus. Collectively, TRPC3 modulates memory and may be a feasible target to enhance memory and treat memory disorders.
publishDate 2015
dc.date.none.fl_str_mv 2015-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/94231
Neuner, Sarah M.; Wilmott, Lynda A.; Hope, Kevin A.; Hoffmann, Brian; Chong, Jayhong A.; et al.; TRPC3 channels critically regulate hippocampal excitability and contextual fear memory; Elsevier Science; Behavioural Brain Research; 281; 3-2015; 69-77
0166-4328
CONICET Digital
CONICET
url http://hdl.handle.net/11336/94231
identifier_str_mv Neuner, Sarah M.; Wilmott, Lynda A.; Hope, Kevin A.; Hoffmann, Brian; Chong, Jayhong A.; et al.; TRPC3 channels critically regulate hippocampal excitability and contextual fear memory; Elsevier Science; Behavioural Brain Research; 281; 3-2015; 69-77
0166-4328
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0166432814008122
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbr.2014.12.018
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842980035350233088
score 12.993085

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