Sex differences in the pituitary transforming growth factor-beta1 (TGF-beta1) system: studies in a model of resistant prolactinomas

Autores
Recouvreux, Maria Victoria; Lapyckyj, Lara; Camilletti, María Andrea; Guida, María Clara; Ornstein, Ana Maria; Rifkin, Daniel B.; Becu, Damasia; Diaz, Graciela Susana
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Dopamine and estradiol interact in the regulation of lactotroph cell proliferation and prolactin secretion. Ablation of the dopamine D2 receptor gene (Drd2 / ) in mice leads to a sexually dimorphic phenotype of hyperprolactinemia and pituitary hyperplasia, which is stronger in females. TGF- 1 is a known inhibitor of lactotroph proliferation. TGF- 1 is regulated by dopamine and estradiol, and it is usually down-regulated in prolactinoma experimental models. To understand the role of TGF- 1 in the gender-specific development of prolactinomas in Drd2 / mice, we compared the expression of different components of the pituitary TGF- 1 system, including active cytokine content, latent TGF- –binding protein isoforms, and possible local TGF- 1 activators, in males and females in this model. Furthermore, we evaluated the effects of dopamine and estradiol administration to elucidate their role in TGF- 1 system regulation. The expression of active TGF- 1, latent TGF- –binding protein isoforms, and several putative TGF- 1 activators evaluated was higher in male than in female mouse pituitary glands. However, Drd2 / female mice were more sensitive to the decrease in active TGF- 1 content, as reflected by the down-regulation of TGF- 1 target genes. Estrogen and dopamine caused differential regulation of several components of the TGF- 1 system. In particular, we found sex- and genotype- dependent regulation of active TGF- 1 content and a similar expression pattern for 2 of the putative TGF- 1 activators, thrombospondin-1 and kallikrein-1, suggesting that these proteins could mediate TGF- 1 activation elicited by dopamine and estradiol. Our results indicate that (1) the loss of dopaminergic tone affects the pituitary TGF- 1 system more strongly in females than in males, (2) males express higher levels of pituitary TGF- 1 system components including active cytokine, and (3) estradiol negatively controls most of the components of the system. Because TGF- 1 inhibits lactotroph proliferation, we propose that the higher levels of the TGF- 1 system in males could protect or delay the development of prolactinomas in Drd2 / male mice.
Fil: Recouvreux, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Lapyckyj, Lara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Camilletti, María Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Guida, María Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Ornstein, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Rifkin, Daniel B.. New York University Medical Center. Department of Cell Biology; Estados Unidos
Fil: Becu, Damasia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Diaz, Graciela Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Materia
Prolactin
Tgf Beta
Pituitary
Sex Differences
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/2212
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/2212
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Sex differences in the pituitary transforming growth factor-beta1 (TGF-beta1) system: studies in a model of resistant prolactinomasRecouvreux, Maria VictoriaLapyckyj, LaraCamilletti, María AndreaGuida, María ClaraOrnstein, Ana MariaRifkin, Daniel B.Becu, DamasiaDiaz, Graciela SusanaProlactinTgf BetaPituitarySex Differenceshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Dopamine and estradiol interact in the regulation of lactotroph cell proliferation and prolactin secretion. Ablation of the dopamine D2 receptor gene (Drd2 / ) in mice leads to a sexually dimorphic phenotype of hyperprolactinemia and pituitary hyperplasia, which is stronger in females. TGF- 1 is a known inhibitor of lactotroph proliferation. TGF- 1 is regulated by dopamine and estradiol, and it is usually down-regulated in prolactinoma experimental models. To understand the role of TGF- 1 in the gender-specific development of prolactinomas in Drd2 / mice, we compared the expression of different components of the pituitary TGF- 1 system, including active cytokine content, latent TGF- –binding protein isoforms, and possible local TGF- 1 activators, in males and females in this model. Furthermore, we evaluated the effects of dopamine and estradiol administration to elucidate their role in TGF- 1 system regulation. The expression of active TGF- 1, latent TGF- –binding protein isoforms, and several putative TGF- 1 activators evaluated was higher in male than in female mouse pituitary glands. However, Drd2 / female mice were more sensitive to the decrease in active TGF- 1 content, as reflected by the down-regulation of TGF- 1 target genes. Estrogen and dopamine caused differential regulation of several components of the TGF- 1 system. In particular, we found sex- and genotype- dependent regulation of active TGF- 1 content and a similar expression pattern for 2 of the putative TGF- 1 activators, thrombospondin-1 and kallikrein-1, suggesting that these proteins could mediate TGF- 1 activation elicited by dopamine and estradiol. Our results indicate that (1) the loss of dopaminergic tone affects the pituitary TGF- 1 system more strongly in females than in males, (2) males express higher levels of pituitary TGF- 1 system components including active cytokine, and (3) estradiol negatively controls most of the components of the system. Because TGF- 1 inhibits lactotroph proliferation, we propose that the higher levels of the TGF- 1 system in males could protect or delay the development of prolactinomas in Drd2 / male mice.Fil: Recouvreux, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Lapyckyj, Lara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Camilletti, María Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Guida, María Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Ornstein, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Rifkin, Daniel B.. New York University Medical Center. Department of Cell Biology; Estados UnidosFil: Becu, Damasia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Diaz, Graciela Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaEndocrine Soc2013-09-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/2212Recouvreux, Maria Victoria; Lapyckyj, Lara; Camilletti, María Andrea; Guida, María Clara; Ornstein, Ana Maria; et al.; Sex differences in the pituitary transforming growth factor-beta1 (TGF-beta1) system: studies in a model of resistant prolactinomas; Endocrine Soc; Endocrinology; 154; 11; 5-9-2013; 4192-42050013-72271945-7170enginfo:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3800752/info:eu-repo/semantics/altIdentifier/url/http://press.endocrine.org/doi/pdf/10.1210/en.2013-1433info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:10:30Zoai:ri.conicet.gov.ar:11336/2212instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:10:30.981CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Sex differences in the pituitary transforming growth factor-beta1 (TGF-beta1) system: studies in a model of resistant prolactinomas
title Sex differences in the pituitary transforming growth factor-beta1 (TGF-beta1) system: studies in a model of resistant prolactinomas
spellingShingle Sex differences in the pituitary transforming growth factor-beta1 (TGF-beta1) system: studies in a model of resistant prolactinomas
Recouvreux, Maria Victoria
Prolactin
Tgf Beta
Pituitary
Sex Differences
title_short Sex differences in the pituitary transforming growth factor-beta1 (TGF-beta1) system: studies in a model of resistant prolactinomas
title_full Sex differences in the pituitary transforming growth factor-beta1 (TGF-beta1) system: studies in a model of resistant prolactinomas
title_fullStr Sex differences in the pituitary transforming growth factor-beta1 (TGF-beta1) system: studies in a model of resistant prolactinomas
title_full_unstemmed Sex differences in the pituitary transforming growth factor-beta1 (TGF-beta1) system: studies in a model of resistant prolactinomas
title_sort Sex differences in the pituitary transforming growth factor-beta1 (TGF-beta1) system: studies in a model of resistant prolactinomas
dc.creator.none.fl_str_mv Recouvreux, Maria Victoria
Lapyckyj, Lara
Camilletti, María Andrea
Guida, María Clara
Ornstein, Ana Maria
Rifkin, Daniel B.
Becu, Damasia
Diaz, Graciela Susana
author Recouvreux, Maria Victoria
author_facet Recouvreux, Maria Victoria
Lapyckyj, Lara
Camilletti, María Andrea
Guida, María Clara
Ornstein, Ana Maria
Rifkin, Daniel B.
Becu, Damasia
Diaz, Graciela Susana
author_role author
author2 Lapyckyj, Lara
Camilletti, María Andrea
Guida, María Clara
Ornstein, Ana Maria
Rifkin, Daniel B.
Becu, Damasia
Diaz, Graciela Susana
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Prolactin
Tgf Beta
Pituitary
Sex Differences
topic Prolactin
Tgf Beta
Pituitary
Sex Differences
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Dopamine and estradiol interact in the regulation of lactotroph cell proliferation and prolactin secretion. Ablation of the dopamine D2 receptor gene (Drd2 / ) in mice leads to a sexually dimorphic phenotype of hyperprolactinemia and pituitary hyperplasia, which is stronger in females. TGF- 1 is a known inhibitor of lactotroph proliferation. TGF- 1 is regulated by dopamine and estradiol, and it is usually down-regulated in prolactinoma experimental models. To understand the role of TGF- 1 in the gender-specific development of prolactinomas in Drd2 / mice, we compared the expression of different components of the pituitary TGF- 1 system, including active cytokine content, latent TGF- –binding protein isoforms, and possible local TGF- 1 activators, in males and females in this model. Furthermore, we evaluated the effects of dopamine and estradiol administration to elucidate their role in TGF- 1 system regulation. The expression of active TGF- 1, latent TGF- –binding protein isoforms, and several putative TGF- 1 activators evaluated was higher in male than in female mouse pituitary glands. However, Drd2 / female mice were more sensitive to the decrease in active TGF- 1 content, as reflected by the down-regulation of TGF- 1 target genes. Estrogen and dopamine caused differential regulation of several components of the TGF- 1 system. In particular, we found sex- and genotype- dependent regulation of active TGF- 1 content and a similar expression pattern for 2 of the putative TGF- 1 activators, thrombospondin-1 and kallikrein-1, suggesting that these proteins could mediate TGF- 1 activation elicited by dopamine and estradiol. Our results indicate that (1) the loss of dopaminergic tone affects the pituitary TGF- 1 system more strongly in females than in males, (2) males express higher levels of pituitary TGF- 1 system components including active cytokine, and (3) estradiol negatively controls most of the components of the system. Because TGF- 1 inhibits lactotroph proliferation, we propose that the higher levels of the TGF- 1 system in males could protect or delay the development of prolactinomas in Drd2 / male mice.
Fil: Recouvreux, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Lapyckyj, Lara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Camilletti, María Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Guida, María Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Ornstein, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Rifkin, Daniel B.. New York University Medical Center. Department of Cell Biology; Estados Unidos
Fil: Becu, Damasia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Diaz, Graciela Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
description Dopamine and estradiol interact in the regulation of lactotroph cell proliferation and prolactin secretion. Ablation of the dopamine D2 receptor gene (Drd2 / ) in mice leads to a sexually dimorphic phenotype of hyperprolactinemia and pituitary hyperplasia, which is stronger in females. TGF- 1 is a known inhibitor of lactotroph proliferation. TGF- 1 is regulated by dopamine and estradiol, and it is usually down-regulated in prolactinoma experimental models. To understand the role of TGF- 1 in the gender-specific development of prolactinomas in Drd2 / mice, we compared the expression of different components of the pituitary TGF- 1 system, including active cytokine content, latent TGF- –binding protein isoforms, and possible local TGF- 1 activators, in males and females in this model. Furthermore, we evaluated the effects of dopamine and estradiol administration to elucidate their role in TGF- 1 system regulation. The expression of active TGF- 1, latent TGF- –binding protein isoforms, and several putative TGF- 1 activators evaluated was higher in male than in female mouse pituitary glands. However, Drd2 / female mice were more sensitive to the decrease in active TGF- 1 content, as reflected by the down-regulation of TGF- 1 target genes. Estrogen and dopamine caused differential regulation of several components of the TGF- 1 system. In particular, we found sex- and genotype- dependent regulation of active TGF- 1 content and a similar expression pattern for 2 of the putative TGF- 1 activators, thrombospondin-1 and kallikrein-1, suggesting that these proteins could mediate TGF- 1 activation elicited by dopamine and estradiol. Our results indicate that (1) the loss of dopaminergic tone affects the pituitary TGF- 1 system more strongly in females than in males, (2) males express higher levels of pituitary TGF- 1 system components including active cytokine, and (3) estradiol negatively controls most of the components of the system. Because TGF- 1 inhibits lactotroph proliferation, we propose that the higher levels of the TGF- 1 system in males could protect or delay the development of prolactinomas in Drd2 / male mice.
publishDate 2013
dc.date.none.fl_str_mv 2013-09-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/2212
Recouvreux, Maria Victoria; Lapyckyj, Lara; Camilletti, María Andrea; Guida, María Clara; Ornstein, Ana Maria; et al.; Sex differences in the pituitary transforming growth factor-beta1 (TGF-beta1) system: studies in a model of resistant prolactinomas; Endocrine Soc; Endocrinology; 154; 11; 5-9-2013; 4192-4205
0013-7227
1945-7170
url http://hdl.handle.net/11336/2212
identifier_str_mv Recouvreux, Maria Victoria; Lapyckyj, Lara; Camilletti, María Andrea; Guida, María Clara; Ornstein, Ana Maria; et al.; Sex differences in the pituitary transforming growth factor-beta1 (TGF-beta1) system: studies in a model of resistant prolactinomas; Endocrine Soc; Endocrinology; 154; 11; 5-9-2013; 4192-4205
0013-7227
1945-7170
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3800752/
info:eu-repo/semantics/altIdentifier/url/http://press.endocrine.org/doi/pdf/10.1210/en.2013-1433
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Endocrine Soc
publisher.none.fl_str_mv Endocrine Soc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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